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Viral Applications Of Green Fluorescent Protein
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Book Synopsis Viral Applications of Green Fluorescent Protein by : Barry W. Hicks
Download or read book Viral Applications of Green Fluorescent Protein written by Barry W. Hicks and published by Humana Press. This book was released on 2009-04-01 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Book Synopsis Green Fluorescent Protein by : Martin Chalfie
Download or read book Green Fluorescent Protein written by Martin Chalfie and published by Wiley-Liss. This book was released on 1998-09-07 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system. This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize expression and enhance detection in a variety of organisms and cell types, generate variants with altered properties, explore new ways to use GFP, and more. Topics covered include: * The discovery of GFP * Bioluminescence and biofluorescence in nature * Biochemical and physical properties of GFP * The three-dimensional structure of GFP and its implications for function and design * Molecular biology and modification of GFP * Uses of GFP in various organisms, including prokaryotes, yeast, C. elegans, drosophila, plants, transgenic vertebrates, and mammalian cells * Detailed protocols for the expression of GFP, specimen preparation, visualization and recording of GFP fluorescence, and GFP websites
Book Synopsis Viral Applications of Green Fluorescent Protein by : Barry W. Hicks
Download or read book Viral Applications of Green Fluorescent Protein written by Barry W. Hicks and published by Humana Press. This book was released on 2009-04-01 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Book Synopsis Green Fluorescent Protein by : Martin Chalfie
Download or read book Green Fluorescent Protein written by Martin Chalfie and published by John Wiley & Sons. This book was released on 2005-11-18 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Book Synopsis Green Fluorescent Protein by : Barry W. Hicks
Download or read book Green Fluorescent Protein written by Barry W. Hicks and published by Humana Press. This book was released on 2002-06-20 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Could there be a better time to be a life scientist? In the past two decades, a host of new techniques have been added to the tool chests of biochemists and molecular biologists. A wonderful benefit of the basic scientific research that fueled the advances in these fields is the wide variety of direct applications in agriculture and medicine. Even with all of these advances, and with the accompanying explosion in computer and information technology, it is clear that the depth of our ignorance vastly exceeds the breadth of our knowledge about complex organisms at the molecular level. Any new techniques or materials that allow us to extend our research-based knowledge should be w- comed and utilized to their fullest potentials. With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented. Although the text has chapters that emphasize different areas of s- cialization, it is not meant to send molecular biologists to one section, botanists to another, and clinicians to still another. Perhaps the most valuable exchange for people in any discipline will come from seeing how others have been able to apply GFP in fields outside of their immediate areas of expertise. GFP from Aequorea victoria is a fluorescent marker protein, and there are certainly other useful fluorophore markers.
Book Synopsis Illuminating Disease by : Marc Zimmer
Download or read book Illuminating Disease written by Marc Zimmer and published by Oxford University Press. This book was released on 2015-01-05 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Green fluorescent proteins have been floating in the ocean for more than 160 million years, but it took a curious scientist, fascinated by pinpricks of green light, to begin unlocking their potential. Now these jellyfish proteins have become one of the most important tools available to researchers in modern medicine and biology. By using them to illuminate other proteins that were previously invisible even under a microscope, scientists are now able to observe facets of disease that would have otherwise gone undetected. Green fluorescent proteins are used in over three million experiments a year and have proved invaluable for tasks such as tracking HIV, breeding bird flu-resistant chickens, and confirming the existence of cancerous stem cells. In Illuminating Disease, Marc Zimmer introduces us to these revolutionary proteins, acquainting readers both with the researchers responsible for the proteins' discovery as well as their wide utility. The book details the history of genetically modified fluorescent parasites and viruses, which provide scientists with lifesaving information about the spread of diseases. Green fluorescent proteins allow scientists and doctors to understand diseases better by quite literally illuminating various microscopic interactions occurring in living cells that otherwise would have gone unseen. The book is richly illustrated, showing the visually striking uses of green fluorescent proteins, and many of these scans have won awards in biological imaging competitions. An ideal introduction for students and advanced researchers alike, Illuminating Disease is an accessible yet deeply probing investigation into one of the most important developments in medical research of the last several decades.
Book Synopsis Fluorescent Proteins II by : Gregor Jung
Download or read book Fluorescent Proteins II written by Gregor Jung and published by Springer Science & Business Media. This book was released on 2012-01-05 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
Download or read book Plant Virology written by Adrian Gibbs and published by Hodder Education. This book was released on 1980 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: The history and scope of plant virology; Some plant viruses and their names; Effects of viruses on plants; Experimental transmission; The composition and structure of the particles of plant viruses; The purification of virus particles, and some properties of purifield preparations; Infectivity assay; Serological methods; Physical and chemical methods of assay and analysis; The effects of inactivators on virus particles; Behaviour of viruses in plants; Variation, strains and classification; Transmission by vectors and in other natural ways; Virus ecology; Ways of preventing crop losses; Viruses of organisms other than higher plants; Origins of viruses; Plant pathogens confused with viruses.
Book Synopsis Cell Biology by the Numbers by : Ron Milo
Download or read book Cell Biology by the Numbers written by Ron Milo and published by Garland Science. This book was released on 2015-12-07 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Book Synopsis Aglow in the Dark by : Vincent Pieribone
Download or read book Aglow in the Dark written by Vincent Pieribone and published by Harvard University Press. This book was released on 2005 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of green fluorescent protein revolutionized molecular biology, transforming our study of everything from the AIDS virus to the workings of the brain.
Book Synopsis Gene Transfer and Expression in Mammalian Cells by : S.C. Makrides
Download or read book Gene Transfer and Expression in Mammalian Cells written by S.C. Makrides and published by Gulf Professional Publishing. This book was released on 2003-10-24 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Glowing Genes written by Marc Zimmer and published by Prometheus Books. This book was released on 2010-04-06 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Marc Zimmer has written the first popular science book on an amazing new area of biotechnology that will help fight cancer, create new products, improve agriculture, and combat terrorism. For more than one hundred and sixty million years, green fluorescent protein has existed in one species of jellyfish. In 1994 it was cloned, giving rise to a host of useful and potentially revolutionary applications in biotechnology. Today researchers are using this ancient glowing protein to pursue exciting new discoveries, from tracking the process of bacterial infection to detecting chemical and biological agents planted by terrorists.A recognized expert in this field, Zimmer begins with an overview of the many uses of these glowing genes to kill and image cancer cells, monitor bacterial infections, and light up in the presence of pollution. He then discusses the biological reasons that glowing proteins first evolved in jellyfish and fireflies, and looks at the history of bioluminescence and the dedicated scientists who devoted their careers to explaining this phenomenon. The story of how "glowing genes" were located, cloned, and then mass-produced is in itself a fascinating tale.Zimmer next turns to the serious, and not-so-serious, uses of fluorescent proteins. In agriculture it may soon be possible to produce crops that signal dryness by glowing. In industry a red fluorescent protein originally found in corals may find a use in sheep as a substitute for environmentally harmful wool dyes.Furthermore, the glowing gene revolution has led to significantly more humane treatment of laboratory animals. No longer must animal lives be sacrificed to understand disease processes; now researchers can observe the spread of cancer and infections by treating animals with green fluorescent genes and similar proteins.In the fight against terrorism a glowing gene has been created that lights up in the presence of anthrax spores, chemical warfare agents, and landmines. And in a completely different arena, we have already seen the emergence of "transgenic art" in Alba, the fluorescent bunny rabbit.Glowing Genes is a highly informative, fascinating, and entertaining read about a burgeoning area of biotechnology that promises soon to revolutionize our world.
Book Synopsis Skeletal Muscle Development by : James G. Ryall
Download or read book Skeletal Muscle Development written by James G. Ryall and published by Humana Press. This book was released on 2017-08-25 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>
Book Synopsis Methods in Plant Cell Biology by : David W. Galbraith
Download or read book Methods in Plant Cell Biology written by David W. Galbraith and published by Academic Press. This book was released on 1995 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.
Book Synopsis Molecular Farming of Plants and Animals for Human and Veterinary Medicine by : L. Erickson
Download or read book Molecular Farming of Plants and Animals for Human and Veterinary Medicine written by L. Erickson and published by Springer Science & Business Media. This book was released on 2013-04-18 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular farming has been hailed as the "third wave" of genetically-modified organisms produced through biotechnology for the bio-based economy of the future. Unlike products of the first wave, such as herbicide resistant crop plants, which were perceived to benefit only the farmers who used them and the agrochemical companies who developed them, products of molecular farming are designed specifically for the benefit of the consumer. Such products could be purified from food or non-food organisms for a range of applications in industry, as well as animal and human health. Alternatively, the products of this technology could be consumed more directly in some edible format, such as milk, eggs, fruits or vegetables. There is a rapidly-growing interest Qn the part of the public as well as in the medical community in the role food plays in health, especially in the immunophysiological impact of food over and above the role of basic nutrition.
Book Synopsis Baculovirus Expression Vectors by : David R. O'Reilly
Download or read book Baculovirus Expression Vectors written by David R. O'Reilly and published by . This book was released on 1994 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.
