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The ELISA Guidebook

Author : John R. Crowther
Publisher : Humana
ISBN 13 : 9781617378843
Total Pages : 0 pages
Book Rating : 4.3/5 (788 download)

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Book Synopsis The ELISA Guidebook by : John R. Crowther

Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.

The Immunoassay Handbook

Author : David Wild
Publisher : Newnes
ISBN 13 : 0080970389
Total Pages : 1037 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis The Immunoassay Handbook by : David Wild

Download or read book The Immunoassay Handbook written by David Wild and published by Newnes. This book was released on 2013-01-21 with total page 1037 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com

Enzyme-linked Immunosorbent Assay (ELISA)

Author : Samira Hosseini
Publisher : Springer
ISBN 13 : 981106766X
Total Pages : 124 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Enzyme-linked Immunosorbent Assay (ELISA) by : Samira Hosseini

Download or read book Enzyme-linked Immunosorbent Assay (ELISA) written by Samira Hosseini and published by Springer. This book was released on 2017-12-30 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

ELISA

Author : Robert Hnasko
Publisher : Humana
ISBN 13 : 9781493927418
Total Pages : 0 pages
Book Rating : 4.9/5 (274 download)

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Book Synopsis ELISA by : Robert Hnasko

Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.

The ELISA Guidebook

Author : John R. Crowther
Publisher : Springer Science & Business Media
ISBN 13 : 1592590497
Total Pages : 426 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis The ELISA Guidebook by : John R. Crowther

Download or read book The ELISA Guidebook written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

ELISA

Author : John R. Crowther
Publisher : Springer Science & Business Media
ISBN 13 : 1592595294
Total Pages : 231 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis ELISA by : John R. Crowther

Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

ELISA

Author : Robert S. Matson
Publisher : Springer Nature
ISBN 13 : 1071629034
Total Pages : 245 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis ELISA by : Robert S. Matson

Download or read book ELISA written by Robert S. Matson and published by Springer Nature. This book was released on 2023-02-16 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.

The Italian Prisoner

Author : Elisa M. Speranza
Publisher : Burgundy Bend Press
ISBN 13 : 1662924143
Total Pages : 246 pages
Book Rating : 4.6/5 (629 download)

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Book Synopsis The Italian Prisoner by : Elisa M. Speranza

Download or read book The Italian Prisoner written by Elisa M. Speranza and published by Burgundy Bend Press. This book was released on 2022-04-11 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1943. New Orleans. Rose Marino lives with her Sicilian immigrant parents and helps in the family grocery store. Her older brother and sister both joined the Army, and Rose prays for their safety as World War II rages overseas. Her parents expect Rose to marry a local boy and start a family. But she secretly dreams of being more like her fiercely independent widowed godmother. Behind her parents’ back, Rose lands a job at the shipyard, where she feels free and important for the first time in her life. When the parish priest organizes a goodwill mission to visit Italian prisoners of war at a nearby military base, Rose and her vivacious best friend, Marie, join the group. There, Rose falls for Sal, a handsome and intelligent POW. Italy has switched sides in the war, so the POWs are allowed out to socialize, giving Rose and Sal a chance to grow closer. When Rose gets a promotion at work, she must make an agonizing choice: follow a traditional path like Marie or keep working after the war and live on her own terms. Inspired by little-known historical events and set to a swing-era soundtrack, The Italian Prisoner is an engrossing story of wartime love, family secrets, and a young woman’s struggle to chart her own course at an inflection point in American history. Book Review 1: “The repercussions of WWII are lovingly rendered through one woman’s story, with an endearing cast of characters who all feel like family by the end.”—LALITA TADEMY, New York Times best-selling author of Oprah’s Book Club pick Cane River, Red River, and Citizens Creek Book Review 2: "… an essential contribution to the treasure trove of Italian American fiction and a transporting page-turner. I want everyone in my family to meet Rose, our inspiring heroine, whose extraordinary story will stay with me for a long time." –CHRISTOPHER CASTELLANI, author of Leading Men Book Review 3: “… intimate historical fiction at its page-turning best.”—PAMELA ROTNER SAKAMOTO, author of Midnight in Broad Daylight: A Japanese American Family Caught Between Two Worlds Book Review 4: “… a compelling mix of freshness and familiarity, using a female rites-of-passage narrative to transport readers to 1940s New Orleans and to bring alive the timeless challenges of living in a nation at war.”—ANN HAGEDORN, award-winning author of Beyond the River, Savage Peace, Sleeper Agent and more Book Review 5: “… a refreshing addition to the historical women’s fiction bookshelf.”—KAIA ALDERSON, author of Sisters in Arms Book Review 6: “A dazzling World War II love story set in New Orleans that will pull you in from the very first paragraph.”—JENNIFER SMITH TURNER, award-winning author of Child Bride, named the Best eBook of 2020 by the Black Caucus of The American Library Association Book Review 7: “… a beautiful book that will touch anyone who ever dared want more out of life.” —CHARLES FORREST JONES, author of The Illusion of Simple Book Review 8: "The author's keen eye for the history of those years are evident as delightful details of the city at war abound.”—BRIAN ALTOBELLO, author of Whiskey, Women, and War: How the Great War Shaped Jim Crow New Orleans Book Review 9: “With true-to-life family dynamics and the drama of first love, the author invites us to take a passeggiata alongside her heroine Rose.”—SHAUNNA J. EDWARDS, co-author of The Thread Collectors Book Review 10: “… compelling, atmospheric, and refined. A truly magnificent read.” —DIANNE C. BRALEY, author of The Silence in the Sound

How This Night Is Different

Author : Elisa Albert
Publisher : Simon and Schuster
ISBN 13 : 074329128X
Total Pages : 229 pages
Book Rating : 4.7/5 (432 download)

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Book Synopsis How This Night Is Different by : Elisa Albert

Download or read book How This Night Is Different written by Elisa Albert and published by Simon and Schuster. This book was released on 2008-02-12 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Titled to reflect the customary question asked at Passover, these ten stories by debut writer Albert explore traditional Jewish rituals with youthful, irreverent exuberance as her characters transition into marriage and child-rearing."--"Publishers Weekly."

Basic Science Methods for Clinical Researchers

Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 383 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

What Are You Doing?

Author : Elisa Amado
Publisher : Groundwood Books Ltd
ISBN 13 : 155498288X
Total Pages : 28 pages
Book Rating : 4.5/5 (549 download)

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Book Synopsis What Are You Doing? by : Elisa Amado

Download or read book What Are You Doing? written by Elisa Amado and published by Groundwood Books Ltd. This book was released on 2017-03-01 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt: A picture book that captures a child’s discovery of the power of reading. Before he leaves for his first day of school, Chepito runs outside to play. He comes across all kinds of people in his neighborhood who are reading. “Why, why, why?” he sings, and they each have a different answer for him, whether it’s a man reading a newspaper, a young girl enjoying a comic, a couple of tourists consulting a guidebook or an archeologist studying hieroglyphics. Later that day, Chepito discovers the fun of reading for himself, and he even brings home a book to share with his little sister. Elisa Amado has written a perfect introduction to the pleasures and uses of the written word. Set in a delightfully retro world by illustrator Manuel Monroy, this book is a true celebration of reading. Correlates to the Common Core State Standards in English Language Arts: CCSS.ELA-LITERACY.RL.K.4 Ask and answer questions about unknown words in a text. CCSS.ELA-LITERACY.RL.1.2 Retell stories, including key details, and demonstrate understanding of their central message or lesson. CCSS.ELA-LITERACY.RL.2.4 Describe how words and phrases (e.g., regular beats, alliteration, rhymes, repeated lines) supply rhythm and meaning in a story, poem, or song.

The Company of Death

Author : Elisa Hansen
Publisher :
ISBN 13 : 9781946926814
Total Pages : 324 pages
Book Rating : 4.9/5 (268 download)

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Book Synopsis The Company of Death by : Elisa Hansen

Download or read book The Company of Death written by Elisa Hansen and published by . This book was released on 2019-01-03 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: The zombie apocalypse was just the beginning of Emily's problems. Now the vampire hunter has to ride cross-country with Death himself in this thrilling apocalyptic adventure series! Two years after the zombie uprising devours 99% of the world's population, it's Emily Campbell's job to destroy vampire communes and rescue their human herds. When a mission goes wrong, and she's faced with certain undeath, she would rather kill herself than ever let them transform her. The problem is that when she tries, the Grim Reaper fails to take her life. And he somehow loses all his powers in the process. Now neither alive nor dead, Emily finds herself stranded in the California desert with Death himself. If she can get him to help her, there's a chance she can cross the vampire- and zombie-swarmed continent to New York, where the remnants of civilization are developing a cure that could make her human again. But without his powers, Death is mired in a crisis of his own. And he is not used to having company.

The Elisa: Enzyme-Linked Immunosorbent Assay in Veterinary Research and Diagnosis

Author : R.C. Wardley
Publisher : Springer Science & Business Media
ISBN 13 : 9789024727698
Total Pages : 352 pages
Book Rating : 4.7/5 (276 download)

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Book Synopsis The Elisa: Enzyme-Linked Immunosorbent Assay in Veterinary Research and Diagnosis by : R.C. Wardley

Download or read book The Elisa: Enzyme-Linked Immunosorbent Assay in Veterinary Research and Diagnosis written by R.C. Wardley and published by Springer Science & Business Media. This book was released on 1982-11-30 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of a Meeting held at the University of Surrey, Guildford, U.K., September 15-17, 1981

After Birth

Author : Elisa Albert
Publisher : Houghton Mifflin Harcourt
ISBN 13 : 0544273737
Total Pages : 211 pages
Book Rating : 4.5/5 (442 download)

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Book Synopsis After Birth by : Elisa Albert

Download or read book After Birth written by Elisa Albert and published by Houghton Mifflin Harcourt. This book was released on 2015 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: A widely acclaimed young writer's fierce new novel, in which childbirth and new motherhood are as high-stakes a crucible as any combat zone.

The Book of Dahlia

Author : Elisa Albert
Publisher : Simon and Schuster
ISBN 13 : 0743291301
Total Pages : 292 pages
Book Rating : 4.7/5 (432 download)

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Book Synopsis The Book of Dahlia by : Elisa Albert

Download or read book The Book of Dahlia written by Elisa Albert and published by Simon and Schuster. This book was released on 2009-03-10 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: A young Jewish-American woman learns that she has brain cancer and through a series of flashbacks examines her wasted life.

Wake Me When You Leave

Author : Elisa Donovan
Publisher : Llewellyn Worldwide
ISBN 13 : 0738768383
Total Pages : 156 pages
Book Rating : 4.7/5 (387 download)

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Book Synopsis Wake Me When You Leave by : Elisa Donovan

Download or read book Wake Me When You Leave written by Elisa Donovan and published by Llewellyn Worldwide. This book was released on 2021-06-08 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Love, Grief, and Healing in Hollywood and Beyond After prominent roles in Clueless; Sabrina, the Teenage Witch; and Beverly Hills 90210; Elisa's career was on the fast track. Until her show is unexpectedly cancelled, her relationship ends, and her father is diagnosed with terminal cancer. This book chronicles Elisa's journey out of despair and heartbreak, with awe-inspiring visitations, dreams, and inexplicable synchronicities that could only be her father letting her know that he's watching over her from the afterlife. Sometimes the universe sends us on a journey that we didn't know we needed. By sharing the lessons and challenges that the universe sent to her, Elisa inspires those who are learning to let go after a loss so they can live again with authenticity, humor, and hope.

Human Blues

Author : Elisa Albert
Publisher : Simon and Schuster
ISBN 13 : 1982167874
Total Pages : 432 pages
Book Rating : 4.9/5 (821 download)

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Book Synopsis Human Blues by : Elisa Albert

Download or read book Human Blues written by Elisa Albert and published by Simon and Schuster. This book was released on 2023-07-11 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Crackling and bighearted...A powerhouse [that] echoes with the truth that we find harmony when we listen first to ourselves.” —Oprah Daily * “Takes off with magnificent speed and never lets up.” —The New York Times * “Revolutionary.” —NPR’s Morning Edition * A Los Angeles Times and Publishers Weekly Best Book of the Year A provocative and “darkly funny” (Cosmopolitan) novel about a woman who desperately wants a child but struggles to accept the use of assisted reproductive technology—a “riotous, visceral” (Vanity Fair) send-up of feminism, fame, art, commerce, and autonomy. On the eve of her fourth album, singer-songwriter Aviva Rosner is plagued by infertility. The twist: as much as Aviva wants a child, she is wary of technological conception, and has poured her ambivalence into her music. As the album makes its way in the world, the shock of the response from fans and critics is at first exciting—and then invasive and strange. Aviva never wanted to be famous, or did she? Meanwhile, her evolving obsession with another iconic musician, gone too soon, might just help her make sense of things. Told over the course of nine menstrual cycles, this utterly original novel is a “fast, fiery, and often funny” (The Boston Globe) interrogation of our cultural obsession with childbearing. It’s also the story of one fearless woman at the crossroads, ruthlessly questioning what she wants and what she’s willing—or not willing—to do to get it.