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The Elisa Guidebook
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Book Synopsis The ELISA Guidebook by : John R. Crowther
Download or read book The ELISA Guidebook written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Book Synopsis The ELISA Guidebook by : John R. Crowther
Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Book Synopsis The Elisa Guidebook by : John R. Crowther
Download or read book The Elisa Guidebook written by John R. Crowther and published by . This book was released on 2007-12-01 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA.
Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Book Synopsis Methods in Molecular Biology: The ELISA guidebook by : John M. Walker
Download or read book Methods in Molecular Biology: The ELISA guidebook written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis What Are You Doing? by : Elisa Amado
Download or read book What Are You Doing? written by Elisa Amado and published by Groundwood Books Ltd. This book was released on 2017-03-01 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt: A picture book that captures a child’s discovery of the power of reading. Before he leaves for his first day of school, Chepito runs outside to play. He comes across all kinds of people in his neighborhood who are reading. “Why, why, why?” he sings, and they each have a different answer for him, whether it’s a man reading a newspaper, a young girl enjoying a comic, a couple of tourists consulting a guidebook or an archeologist studying hieroglyphics. Later that day, Chepito discovers the fun of reading for himself, and he even brings home a book to share with his little sister. Elisa Amado has written a perfect introduction to the pleasures and uses of the written word. Set in a delightfully retro world by illustrator Manuel Monroy, this book is a true celebration of reading. Correlates to the Common Core State Standards in English Language Arts: CCSS.ELA-LITERACY.RL.K.4 Ask and answer questions about unknown words in a text. CCSS.ELA-LITERACY.RL.1.2 Retell stories, including key details, and demonstrate understanding of their central message or lesson. CCSS.ELA-LITERACY.RL.2.4 Describe how words and phrases (e.g., regular beats, alliteration, rhymes, repeated lines) supply rhythm and meaning in a story, poem, or song.
Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Book Synopsis Molecular Biomethods Handbook by : John M. Walker
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Book Synopsis The Enzyme Linked Immunosorbent Assay (Elisa) by : A. Voller
Download or read book The Enzyme Linked Immunosorbent Assay (Elisa) written by A. Voller and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Book Synopsis Enzyme-linked Immunosorbent Assay (ELISA) by : Samira Hosseini
Download or read book Enzyme-linked Immunosorbent Assay (ELISA) written by Samira Hosseini and published by Springer. This book was released on 2018-01-22 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
Book Synopsis The ELISA Guidebook by : John R. Crowther
Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2000-08-17 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Book Synopsis Kirk & Bistner's Handbook of Veterinary Procedures and Emergency Treatment - E-Book by : Richard B. Ford
Download or read book Kirk & Bistner's Handbook of Veterinary Procedures and Emergency Treatment - E-Book written by Richard B. Ford and published by Elsevier Health Sciences. This book was released on 2011-08-22 with total page 784 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provide expert care for cats and dogs! Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment, 9th Edition covers not only the management of emergency conditions, but also strategies for dealing with hundreds of routine diagnostic and treatment challenges in small animals. Its user-friendly format provides instant access to vital information -- making it an ideal resource in emergency situations -- and it is conveniently organized by both body systems and presenting signs to help you easily reach a diagnosis and determine a treatment plan for all clinical situations. Written by veterinary experts Richard Ford and Elisa Mazzaferro, Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment provides current guidelines for small animal emergency care and the diagnostic procedures most commonly performed in a busy, team-oriented practice. Step-by-step instructions and illustrations are provided for all major emergency and non-emergency clinical procedures. A logical, easy-to-use format lists all emergency conditions in alphabetical order, and includes quick reference boxes calling out key information such as clinical tips and cautions. Clear, concise guidelines help you evaluate clinical signs and laboratory test data. Clinical algorithms make it easier to identify and treat abnormalities. Guidelines for assessment and treatment include practical advice and solutions, how to examine the small animal patient using a body systems and problem list approach, and a review of basic diagnostic procedures used in daily practice. Coverage of toxicological emergencies describes how to manage exposures and poisonings. A quick reference guide to the management of the emergency patient is conveniently located on the inside cover. A comprehensive drug formulary makes lookup easy, and includes proprietary names, actions/use of each drug, formulations, recommended dosages, and special precautions, with emergency medications highlighted for fast reference This all-in-one reference includes practical coverage of emergency procedures, physical assessment in sickness and health, routine and advanced testing procedures, diagnostic tests sampling, preparation, procedures, and interpretation. Quick Reference boxes include potential causes of each clinical abnormality and associated signs, step-by-step diagnostic plans, and clinical algorithms. The latest vaccination guidelines include protocols for dogs and cats at low, medium, and high risk of exposure to infectious diseases. Updated coverage keeps you current with the latest on pain assessment, prevention, and treatment.
Book Synopsis Fanfares and Finesse by : Elisa Koehler
Download or read book Fanfares and Finesse written by Elisa Koehler and published by Indiana University Press. This book was released on 2014-02-27 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: “A concise guide linking the history of trumpet to performance . . . includes information on band music, bugle calls, orchestral repertoire, and jazz.” —American Reference Books Annual Unlike the violin, which has flourished largely unchanged for close to four centuries, the trumpet has endured numerous changes in design and social status from the battlefield to the bandstand and ultimately to the concert hall. This colorful past is reflected in the arsenal of instruments a classical trumpeter employs during a performance, sometimes using no fewer than five in different keys and configurations to accurately reproduce music from the past. With the rise in historically inspired performances comes the necessity for trumpeters to know more about their instrument’s heritage, its repertoire, and different performance practices for old music on new and period-specific instruments. More than just a history of the trumpet, this essential reference book is a comprehensive guide for musicians who bring that musical history to life. “A compendium of trumpet history with short, fact-filled chapters. It will serve both amateur and professional musicians alike, and few could read this text without learning something. Fanfares and Finesse is a thorough sampling of trumpet topics, including something of interest for every trumpet player, brass enthusiast, or curious reader.” —Pan Pipes “Trumpet players in a wide variety of situations and at many levels will find a great deal of useful information, presented in a clear, engaging, reader-friendly way yet backed by solid research. While some topics are covered in more depth than others, Koehler’s breadth of vision and thoroughness are commendable . . . For all trumpeters and anyone who teaches them.” —Choice
Download or read book Elisa's Diary written by Elisa Velasquez and published by Createspace Independent Publishing Platform. This book was released on 2017-02-11 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Excerpts from Elisa's personal diary of her conversations with God. I'm always writing, God is constantly talking and I'm always writing. This book is uniquely wonderful and penned with my heart. I love sharing what I write and the words that God whispers in my ear. It's important to build people up and inspire and even correct. This diary does just that. Elisa spraks to your spirit heart and delivers God's Messages purely and honest. Listen to God's word. Elisa's Diary are excerpts from her daily personal God diary. Keeping a diary is most rewarding and memorable. Your thoughts are probably the best legacies to leave behind on earth.
Book Synopsis Winter Wishes (Disney Princess) by : Apple Jordan
Download or read book Winter Wishes (Disney Princess) written by Apple Jordan and published by RH/Disney. This book was released on 2013-11-27 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt: Share the wintry wishes and dreams of Cinderella, Ariel, and all the Disney Princesses in this sweet step 2, Step into Reading. For the first time, the Princesses come together in a seasonal story that is bound to have young readers learning to read while making their very own winter wish!
Book Synopsis Do You Draw Pictures? by : Becki C. Lee
Download or read book Do You Draw Pictures? written by Becki C. Lee and published by Scarlet Oak Press. This book was released on 2020-10-01 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intellectual property affects everyone. Even from an early age, children consume books, movies, and music, and start to recognize logos. Kids are not likely to learn about trademarks, patents, and copyrights in grade school, so they go by what they hear in pop culture. That information is often flawed. As children grow into young adults, they become songwriters, authors, artists, and entrepreneurs who could use intellectual property to support their dreams. But there is a barrier to entry in terms of truly understanding their rights, because only lawyers are formally trained to navigate the issues. Our children deserve to be empowered by learning the basics about copyrights, trademarks, and patents as early as they can. Do You Draw Pictures? is a small step in that direction, using easy-to-understand language and fun illustrations to help kids understand intellectual property and how those rights apply to their own lives. Pick up a copy today so you can help the future generation of artists, inventors, and entrepreneurs learn to protect their creations.
