Super Resolution Microscopy

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Superresolution Optical Microscopy

Author : Barry R. Masters
Publisher : Springer
ISBN 13 : 9783030216931
Total Pages : 0 pages
Book Rating : 4.2/5 (169 download)

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Book Synopsis Superresolution Optical Microscopy by : Barry R. Masters

Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer. This book was released on 2021-08-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Label-Free Super-Resolution Microscopy

Author : Vasily Astratov
Publisher : Springer Nature
ISBN 13 : 3030217221
Total Pages : 487 pages
Book Rating : 4.0/5 (32 download)

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Book Synopsis Label-Free Super-Resolution Microscopy by : Vasily Astratov

Download or read book Label-Free Super-Resolution Microscopy written by Vasily Astratov and published by Springer Nature. This book was released on 2019-08-31 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Super-Resolution Microscopy

Author : Udo J. Birk
Publisher : John Wiley & Sons
ISBN 13 : 3527341331
Total Pages : 405 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Super-Resolution Microscopy by : Udo J. Birk

Download or read book Super-Resolution Microscopy written by Udo J. Birk and published by John Wiley & Sons. This book was released on 2017-12-04 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Super-Resolution Microscopy

Author : Udo J. Birk
Publisher : John Wiley & Sons
ISBN 13 : 3527802061
Total Pages : 260 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Super-Resolution Microscopy by : Udo J. Birk

Download or read book Super-Resolution Microscopy written by Udo J. Birk and published by John Wiley & Sons. This book was released on 2017-07-21 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Fluorescence Microscopy

Author : Anda Cornea
Publisher : Elsevier
ISBN 13 : 0124167136
Total Pages : 260 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Fluorescence Microscopy by : Anda Cornea

Download or read book Fluorescence Microscopy written by Anda Cornea and published by Elsevier. This book was released on 2014-02-24 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Super-Resolution Imaging in Biomedicine

Author : Alberto Diaspro
Publisher : CRC Press
ISBN 13 : 1315355566
Total Pages : 525 pages
Book Rating : 4.3/5 (153 download)

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Book Synopsis Super-Resolution Imaging in Biomedicine by : Alberto Diaspro

Download or read book Super-Resolution Imaging in Biomedicine written by Alberto Diaspro and published by CRC Press. This book was released on 2016-11-03 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Recent Advances in Fluorescent Probes for Super-Resolution Microscopy

Author : Chayan Kanti Nandi
Publisher : Frontiers Media SA
ISBN 13 : 2889710718
Total Pages : 105 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Recent Advances in Fluorescent Probes for Super-Resolution Microscopy by : Chayan Kanti Nandi

Download or read book Recent Advances in Fluorescent Probes for Super-Resolution Microscopy written by Chayan Kanti Nandi and published by Frontiers Media SA. This book was released on 2021-07-21 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

High Resolution Imaging in Microscopy and Ophthalmology

Author : Josef F. Bille
Publisher : Springer
ISBN 13 : 3030166384
Total Pages : 407 pages
Book Rating : 4.0/5 (31 download)

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Book Synopsis High Resolution Imaging in Microscopy and Ophthalmology by : Josef F. Bille

Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Super-Resolution Imaging in Biomedicine

Author : Alberto Diaspro
Publisher : CRC Press
ISBN 13 : 1482244357
Total Pages : 426 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Super-Resolution Imaging in Biomedicine by : Alberto Diaspro

Download or read book Super-Resolution Imaging in Biomedicine written by Alberto Diaspro and published by CRC Press. This book was released on 2016-11-03 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Advanced Microscopy in Mycology

Author : Tanya E. S. Dahms
Publisher : Springer
ISBN 13 : 3319224379
Total Pages : 164 pages
Book Rating : 4.3/5 (192 download)

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Book Synopsis Advanced Microscopy in Mycology by : Tanya E. S. Dahms

Download or read book Advanced Microscopy in Mycology written by Tanya E. S. Dahms and published by Springer. This book was released on 2015-10-30 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this volume is to describe the latest advances in microscopic methods, including integrated techniques, as applied to mycology. Each chapter will provide a brief overview of a particular microscopic method with associated advantages and limitations, the research questions that can be appropriately addressed using these microscopic methods, how it has been successfully applied to address mycological research questions, including supporting and complimentary techniques, and which future questions can be addressed.

Super-Resolution Microscopy

Author : Holger Erfle
Publisher :
ISBN 13 : 9781493984350
Total Pages : pages
Book Rating : 4.9/5 (843 download)

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Book Synopsis Super-Resolution Microscopy by : Holger Erfle

Download or read book Super-Resolution Microscopy written by Holger Erfle and published by . This book was released on 2018-08-13 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Far-Field Optical Nanoscopy

Author : Philip Tinnefeld
Publisher : Springer
ISBN 13 : 3662455471
Total Pages : 335 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Far-Field Optical Nanoscopy by : Philip Tinnefeld

Download or read book Far-Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Plasmonics and Super-Resolution Imaging

Author : Zhaowei Liu
Publisher : CRC Press
ISBN 13 : 1351797328
Total Pages : 482 pages
Book Rating : 4.3/5 (517 download)

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Book Synopsis Plasmonics and Super-Resolution Imaging by : Zhaowei Liu

Download or read book Plasmonics and Super-Resolution Imaging written by Zhaowei Liu and published by CRC Press. This book was released on 2017-06-26 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmonics is an emerging field mainly developed within the past two decades. Due to its unique capabilities to manipulate light at deep subwavelength scales, plasmonics has been commonly treated as the most important part of nanophotonics. Plasmonic-assisted optical microscopy techniques, especially super-resolution microscopy, have shown tremendous potential and attracted much attention. This book aims to collect cutting-edge studies in various optical imaging technologies with advanced performances that are enabled or enhanced by plasmonics. The basic working principles, development details, and potential future direction and perspectives are discussed. Edited by Zhaowei Liu, a prominent researcher in the field of super-resolution microscopy, this book will be an excellent reference for anyone in the field of nanophotonics, plasmonics, and optical microscopy.

Superresolution Optical Microscopy

Author : Barry R. Masters
Publisher : Springer Nature
ISBN 13 : 3030216918
Total Pages : 415 pages
Book Rating : 4.0/5 (32 download)

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Book Synopsis Superresolution Optical Microscopy by : Barry R. Masters

Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

High-Resolution Electron Microscopy

Author : John C. H. Spence
Publisher : Oxford University Press
ISBN 13 : 0199552754
Total Pages : 425 pages
Book Rating : 4.1/5 (995 download)

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Book Synopsis High-Resolution Electron Microscopy by : John C. H. Spence

Download or read book High-Resolution Electron Microscopy written by John C. H. Spence and published by Oxford University Press. This book was released on 2009 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes how to see atoms using electron microscopes. This new edition includes updated sections on applications and new uses of atomic-resolution transmission electron microscopy. Several new chapters and sources of software for image interpretation and electron-optical design have also been added.

Confocal Microscopy and Multiphoton Excitation Microscopy

Author : Barry R. Masters
Publisher : SPIE Press
ISBN 13 : 9780819461186
Total Pages : 236 pages
Book Rating : 4.4/5 (611 download)

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Book Synopsis Confocal Microscopy and Multiphoton Excitation Microscopy by : Barry R. Masters

Download or read book Confocal Microscopy and Multiphoton Excitation Microscopy written by Barry R. Masters and published by SPIE Press. This book was released on 2006 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.

Expansion Microscopy for Cell Biology

Author :
Publisher : Academic Press
ISBN 13 : 0128208082
Total Pages : 358 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Expansion Microscopy for Cell Biology by :

Download or read book Expansion Microscopy for Cell Biology written by and published by Academic Press. This book was released on 2021-01-19 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more. Provides the authority and expertise of leading contributors from an international board of authors Represents the latest release in the Methods in Cell Biology series Includes the latest information on Expansion Microscopy for Cell Biology