Label-Free Super-Resolution Microscopy

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Author :
Publisher : Springer Nature
ISBN 13 : 3030217221
Total Pages : 498 pages
Book Rating : 4.0/5 (32 download)

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Book Synopsis Label-Free Super-Resolution Microscopy by : Vasily Astratov

Download or read book Label-Free Super-Resolution Microscopy written by Vasily Astratov and published by Springer Nature. This book was released on 2019-08-31 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Far-Field Optical Nanoscopy

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Author :
Publisher : Springer
ISBN 13 : 3662455471
Total Pages : 340 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Far-Field Optical Nanoscopy by : Philip Tinnefeld

Download or read book Far-Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Correlative Light and Electron MIcroscopy

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Publisher : Academic Press
ISBN 13 : 0124160263
Total Pages : 466 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Correlative Light and Electron MIcroscopy by : Thomas Muller-Reichert

Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

Stimulated Raman Scattering Microscopy

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Publisher : Elsevier
ISBN 13 : 0323903371
Total Pages : 612 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Stimulated Raman Scattering Microscopy by : Ji-Xin Cheng

Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. - Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy - Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics - Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics

Single Molecule Spectroscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 3642565441
Total Pages : 375 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Single Molecule Spectroscopy by : R. Rigler

Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Super-Resolution Microscopy

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527341331
Total Pages : 405 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Super-Resolution Microscopy by : Udo J. Birk

Download or read book Super-Resolution Microscopy written by Udo J. Birk and published by John Wiley & Sons. This book was released on 2017-12-04 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Fourier Ptychographic Imaging

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Publisher : Morgan & Claypool Publishers
ISBN 13 : 168174273X
Total Pages : 94 pages
Book Rating : 4.6/5 (817 download)

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Book Synopsis Fourier Ptychographic Imaging by : Guoan Zheng

Download or read book Fourier Ptychographic Imaging written by Guoan Zheng and published by Morgan & Claypool Publishers. This book was released on 2016-06-30 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book demonstrates the concept of Fourier ptychography, a new imaging technique that bypasses the resolution limit of the employed optics. In particular, it transforms the general challenge of high-throughput, high-resolution imaging from one that is coupled to the physical limitations of the optics to one that is solvable through computation. Demonstrated in a tutorial form and providing many MATLAB® simulation examples for the reader, it also discusses the experimental implementation and recent developments of Fourier ptychography. This book will be of interest to researchers and engineers learning simulation techniques for Fourier optics and the Fourier ptychography concept.

Microscopy of the Heart

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Author :
Publisher : Springer
ISBN 13 : 3319953044
Total Pages : 135 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis Microscopy of the Heart by : Lars Kaestner

Download or read book Microscopy of the Heart written by Lars Kaestner and published by Springer. This book was released on 2018-12-07 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides in depths information on different microscopy approaches and supplies the reader with methods how to untangle highly complex processes involved in physiological and pathophysiological cardiac signaling. Microscopy approaches have established themselves as the quasi gold standard that enables us to appreciate the underlying mechanisms of physiological and pathophysiological cardiac signaling. This book presents the most important microscopy techniques from the level of individual molecule e.g. Förster-Resonance Energy Transfer (FRET), up to cellular and tissue imaging, e.g. electron microscopy (TEM) or light sheet microscopy. The book is intended for graduate students and postdocs in cardiovascular research, imaging and cell biology, pre-clinical and clinical researchers in cardiovascular sciences as well as decision makers of the pharmaceutical industry.

Nanoscale Photonic Imaging

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Publisher : Springer Nature
ISBN 13 : 3030344134
Total Pages : 634 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Nanoscale Photonic Imaging by : Tim Salditt

Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.

Optical Imaging and Sensing

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Publisher : John Wiley & Sons
ISBN 13 : 3527835199
Total Pages : 293 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Optical Imaging and Sensing by : Jiang Wu

Download or read book Optical Imaging and Sensing written by Jiang Wu and published by John Wiley & Sons. This book was released on 2023-08-03 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical Imaging and Sensing Understand the future of optical imaging with this cutting-edge guide Optoelectronic devices for imaging and sensing are among the backbones of modern technology. Facilitating the mutual conversion of optical and electrical signals, they have applications from telecommunications to molecular spectroscopy, and their incorporation into photon-involved technologies is only growing. The rapid development of this field makes the need for a fully up-to-date introduction all the more critical. Optical Imaging and Sensing meets this need with a comprehensive guide to the novel materials and devices employed in optical imaging and sensing. Given the current revolution in new imaging materials, an introduction that fully incorporates the latest research is an indispensable tool for scientists and engineers in a huge range of fields. The technologies surveyed here promise to transform public security, 5G and next-generation wireless communication, clinical imaging, and many more. Optical Imaging and Sensing Readers will also find: Detailed discussion of materials including semimetallic graphene, semiconducting black phosphorous, and many more Discussion of devices from infrared photodetectors to nonlinear interferometers A thorough look forward to the future of the field Optical Imaging and Sensing is a useful reference for materials scientists, spectroscopists, semiconductor physicists, and engineers working in any field or industry involving optical imaging or sensing technology.

Engineering Optics 2.0

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Publisher : Springer
ISBN 13 : 9811357552
Total Pages : 701 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Engineering Optics 2.0 by : Xiangang Luo

Download or read book Engineering Optics 2.0 written by Xiangang Luo and published by Springer. This book was released on 2019-02-28 with total page 701 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides comprehensive information on the history and status quo of a new research field, which we refer to as Engineering Optics 2.0. The content covers both the theoretical basis and the engineering aspects in connection with various applications. The field of Engineering Optics employs optical theories to practical applications in a broad range of areas. However, the foundation of traditional Engineering Optics was formed several hundred years ago, and the field has developed only very gradually. With technological innovations in both the fabrication and characterization of microstructures, the past few decades have witnessed many groundbreaking changes to the bases of optics, including the generalizing of refraction, reflection, diffraction, radiation and absorption theories. These new theories enable us to break through the barriers in traditional optical technologies, yielding revolutionary advances in traditional optical systems such as microscopes, telescopes and lithography systems.

Optical Measurements for Scientists and Engineers

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Publisher : Cambridge University Press
ISBN 13 : 1107173019
Total Pages : 321 pages
Book Rating : 4.1/5 (71 download)

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Book Synopsis Optical Measurements for Scientists and Engineers by : Arthur McClelland

Download or read book Optical Measurements for Scientists and Engineers written by Arthur McClelland and published by Cambridge University Press. This book was released on 2018-04-19 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible, introductory text explaining how to select, set up and use optical spectroscopy and optical microscopy techniques.

Super Resolution Optical Imaging and Microscopy

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Publisher : John Wiley & Sons
ISBN 13 : 3527349863
Total Pages : 261 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Super Resolution Optical Imaging and Microscopy by : Junle Qu

Download or read book Super Resolution Optical Imaging and Microscopy written by Junle Qu and published by John Wiley & Sons. This book was released on 2023-12-11 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.

Label-Free Monitoring of Cells in vitro

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Author :
Publisher : Springer Nature
ISBN 13 : 3030324338
Total Pages : 286 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Label-Free Monitoring of Cells in vitro by : Joachim Wegener

Download or read book Label-Free Monitoring of Cells in vitro written by Joachim Wegener and published by Springer Nature. This book was released on 2019-11-18 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.

The Photonic Hook

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Publisher : Springer Nature
ISBN 13 : 3030669459
Total Pages : 104 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis The Photonic Hook by : Oleg V. Minin

Download or read book The Photonic Hook written by Oleg V. Minin and published by Springer Nature. This book was released on 2021-03-31 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the recently-discovered artificially curved light beam known as the photonic hook. Self-bending of light, a long-time goal of optical scientists, was realized in 2007 with the Airy beam, followed by the first demonstration of the photonic hook by the authors of this book and their collaborators in 2015 and experimentally in 2019. The photonic hook has curvature less than the wavelength, along with other unique features described in this book that are not shared by Airy-like beams, and so deepens our understanding of light propagation. This book discusses the general principles of artificial near-field structured curved light and the full-wave simulations of the photonic hook along with their experimental confirmation. The book goes on to show how the photonic hook has implications for acoustic and surface plasmon waves and as well as applications in nanoparticle manipulation.

Coherent Raman Scattering Microscopy

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Author :
Publisher : CRC Press
ISBN 13 : 1439867666
Total Pages : 603 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Coherent Raman Scattering Microscopy by : Ji-Xin Cheng

Download or read book Coherent Raman Scattering Microscopy written by Ji-Xin Cheng and published by CRC Press. This book was released on 2016-04-19 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc

MALDI Mass Spectrometry Imaging

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Publisher : Royal Society of Chemistry
ISBN 13 : 1839162414
Total Pages : 541 pages
Book Rating : 4.8/5 (391 download)

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Book Synopsis MALDI Mass Spectrometry Imaging by : Tiffany Siegel Porta

Download or read book MALDI Mass Spectrometry Imaging written by Tiffany Siegel Porta and published by Royal Society of Chemistry. This book was released on 2021-12-03 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.