Protocol for Somatic Embryogenesis in Woody Plants

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1402029853
Total Pages : 580 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Protocol for Somatic Embryogenesis in Woody Plants by : Shri Mohan Jain

Download or read book Protocol for Somatic Embryogenesis in Woody Plants written by Shri Mohan Jain and published by Springer Science & Business Media. This book was released on 2005-08-26 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

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Author :
Publisher : Springer
ISBN 13 : 3319894838
Total Pages : 323 pages
Book Rating : 4.3/5 (198 download)

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Book Synopsis Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants by : Shri Mohan Jain

Download or read book Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants written by Shri Mohan Jain and published by Springer. This book was released on 2018-06-11 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

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Author :
Publisher : Springer
ISBN 13 : 3319790870
Total Pages : 392 pages
Book Rating : 4.3/5 (197 download)

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Book Synopsis Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants by : Shri Mohan Jain

Download or read book Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants written by Shri Mohan Jain and published by Springer. This book was released on 2018-05-30 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Somatic Embryogenesis

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Author :
Publisher : Springer Nature
ISBN 13 : 1071624857
Total Pages : 272 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Somatic Embryogenesis by : Marco A. Ramírez-Mosqueda

Download or read book Somatic Embryogenesis written by Marco A. Ramírez-Mosqueda and published by Springer Nature. This book was released on 2022-08-11 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of methods for plant species, mainly species with high economic value, to achieve in vitro propagation through somatic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species to this morphogenetic route. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Embryogenesis: Methods and Protocols aims to guide future researcher to achieve success in obtaining and regenerating somatic embryos. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Somatic Embryogenesis in Woody Plants

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9401730326
Total Pages : 549 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S.M. Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S.M. Jain and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid progress made on somatic embryogenesis and its prospects for potential applications in improving woody plants prompted us to edit this book initially in three volumes, and now to add two more volumes. The editors were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This Volume 4 has been divided into three sections and contains 23 chapters. Section A contains eleven chapters covering studies of embryo development and cell biology of white spruce, proliferative somatic embryogenesis in woody species, somatic embryo germination and desiccation tolerance in conifers, performance of conifer somatic seedlings, apoptosis during early somatic embryogenesis, water relation parameters in conifer embryos, image analysis of somatic embryos, somatic embryogenesis in woody legumes, cold storage and cryopreservation, and commercialization of plant somatic embryogenesis. Section B contains six chapters dealing with angiosperm woody plants, such as somatic embryogenesis in myrtaceous plants, Laurus nobilis, Simarouba glauca, Magnolia spp., Juglans cinera, and somatic embryogenesis and evaluation of variability in somatic seedlings of Quercus serrata by RAPD markers. The chapters contained in Section C are focused on somatic embryogenesis in gymnosperms, including Pinus patula, Encephalartos, Picea wilsonii, Pinus banksiana, hybrid firs, and Taxus. All the chapters have been peer-reviewed and revised accordingly to improve their quality.

Somatic Embryogenesis in Woody Plants

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Publisher : Springer Science & Business Media
ISBN 13 : 9401109605
Total Pages : 385 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S. Mohan Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S. Mohan Jain and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: These books provide an update to progress on somatic embryogenesis in woody plants including both angiosperm and gymnosperm trees. In the past, most of the information on this subject was scattered in proceedings volumes, journals, biotechnology books, etc. It has been difficult for the researchers and students to obtain comprehensive information on this rapidly growing subject from a single source. These books enable readers to get a clear view of this subject on historical, anatomical, physiological, biochemical and molecular aspects, and applications including protoplasts, cryopreservation, manufactured seed (artificial seed), genetic transformation, bioreactors, mutations, and future uses in forest plantations. Each selected woody plant mentioned in the book is briefly introduced first, covering botany and genetics, importance and geographical distribution, breeding problems, and in vitro propagation and problems of each selected woody plant and then is followed by the description on the initiation and maintenance of embryogenic cultures, embryo development and germination, and field trials (if any) of these plants. These books are meant for graduate students and researchers in forestry and horticulture as well as biotechnologists.

Somatic Embryogenesis in Woody Plants

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9401147744
Total Pages : 338 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S.M. Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S.M. Jain and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environmental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding could become a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Generally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus and mango (Mangifera indica), offer a ray of hope for inexpensive clonal propagation for large-scale production of plants or 'emblings' or somatic seedlings; protoplast work; cryopreservation; genetic transformation; and synthetic or artificial or manufactured seed production.

Somatic Embryogenesis in Woody Plants

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9780792364191
Total Pages : 768 pages
Book Rating : 4.3/5 (641 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S. Mohan Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S. Mohan Jain and published by Springer Science & Business Media. This book was released on 2000-09-30 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid progress on somatic embryogenesis and its prospects for potential application to improving woody plants prompted us to edit this book initially in three volumes, and now an additional three more volumes. We were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This volume 6 is dedicated to Prof. Harry Waris, Helsinki, Finland, who did pioneer work on somatic embryogenesis during the time when Prof. Steward and others were actively engaged in this area. His former student Prof. Liisa Simols, University of Helsinki, Finland, has written a dedication `Harry Waris, a pioneer in somatic embryogenesis' to her teacher Prof. Waris. This volume is divided into three sections and contains a total of 26 chapters. Section A comprises seven chapters covering topics such as: Historical insights into some contemporary problems in somatic embryogenesis (SE); Thin cell layer for somatic embryogenesis induction in woody trees; SE in tropical fruit and forest trees; SE in fruit and forest arid trees; Status of SE in Indian forest trees; SE research in fruit trees in India; Applications of SE for the improvement of tropical fruit trees. Section B comprises 15 chapters, dealing with: SE in oil palm, hazelnut (Corylus avellana L.), pistachio (Pistacia vera L.), Araucaria angustifolia, Quercus suber, Aspidosperma polyneuron, Acacia senegal, Simmondsia chiensis, Cupressus sempervirens, pecan (Carya illinoinensis), rattan (Calamus spp.), tamarillo (Cyphomandra betacea, longan (Dimocarpus longan Lor.), Aegle marmelos, and Euonymus europaeus. Section C comprises three chapters related to cryo-storage of citrus, conifers and rubber. All the chapters have been peer-reviewed and revised accordingly to improve the quality of the chapters. We are thankful to all: (a) contributory authors for their co-operation in submitting manuscripts in time, and (b) reviewers for spending their valuable time in reviewing the manuscripts.

Somatic Embryogenesis in Woody Plants

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Publisher : Springer Science & Business Media
ISBN 13 : 9401104913
Total Pages : 512 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S. Mohan Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S. Mohan Jain and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com mercial companies. Generally, tissue culture of woody plants has been recal citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalurn album), Citrus, Mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propa gation for large-scale production of plants or "emblings" or "somatic embryo plants", b) protoplast work, c) cryopreservation, d) genetic transformation, and e) artificial or manufactured seed production.

Protocols for Micropropagation of Woody Trees and Fruits

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1402063520
Total Pages : 559 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Protocols for Micropropagation of Woody Trees and Fruits by : S.Mohan Jain

Download or read book Protocols for Micropropagation of Woody Trees and Fruits written by S.Mohan Jain and published by Springer Science & Business Media. This book was released on 2007-09-18 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

Somatic Embryogenesis in Woody Plants

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 940173030X
Total Pages : 743 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S.M. Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S.M. Jain and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 743 pages. Available in PDF, EPUB and Kindle. Book excerpt: The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.

Somatic Embryogenesis in Woody Plants

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Publisher : Springer
ISBN 13 :
Total Pages : 416 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Somatic Embryogenesis in Woody Plants by : S. Mohan Jain

Download or read book Somatic Embryogenesis in Woody Plants written by S. Mohan Jain and published by Springer. This book was released on 1995-02-28 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously un exploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com- mercial companies. Generally, tissue culture of woody plants has been recal- citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus, Mango (Mangifera indica), etc. offer a ray of hope of: (a) inexpensive clonal propa- gation for large-scale production of plants or "emblings" or "somatic embryo plants"; (b) protoplast work; (c) cryopreservation; (d) genetic transforma- tion; and (e) artificial or manufactured seed production.

Protocol for Somatic Embryogenesis in Woody Plants

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9781402029844
Total Pages : 604 pages
Book Rating : 4.0/5 (298 download)

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Book Synopsis Protocol for Somatic Embryogenesis in Woody Plants by : Shri Mohan Jain

Download or read book Protocol for Somatic Embryogenesis in Woody Plants written by Shri Mohan Jain and published by Springer Science & Business Media. This book was released on 2005-05-23 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Somatic Embryogenesis: Fundamental Aspects and Applications

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Author :
Publisher : Springer
ISBN 13 : 331933705X
Total Pages : 506 pages
Book Rating : 4.3/5 (193 download)

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Book Synopsis Somatic Embryogenesis: Fundamental Aspects and Applications by : Víctor M Loyola-Vargas

Download or read book Somatic Embryogenesis: Fundamental Aspects and Applications written by Víctor M Loyola-Vargas and published by Springer. This book was released on 2016-06-06 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses basic and applied aspects of somatic embryogenesis, one of the most powerful tools in plant biotechnology. It is divided into three parts; Part I includes topics such as the history of this research field, how differentiated plant cells can (re)acquire totipotency, molecular features, as well as the epigenetics and proteomics of somatic embryogenesis. Part II covers the somatic embryogenesis of different crops, such as Agave spp. maize, Cocos nucifera, Bixa orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and Arabidopsis thaliana. Various applications, like scale-up propagation and genetic engineering are discussed in detail in Part III. The book will appeal to plant scientists, plant breeders and experts working in industry.

Somatic Embryogenesis in Woody Plants: without special title

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (94 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants: without special title by : S. Mohan Jain

Download or read book Somatic Embryogenesis in Woody Plants: without special title written by S. Mohan Jain and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Somatic Embryogenesis in Woody Plants: Gymnosperms

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (94 download)

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Book Synopsis Somatic Embryogenesis in Woody Plants: Gymnosperms by : S. Mohan Jain

Download or read book Somatic Embryogenesis in Woody Plants: Gymnosperms written by S. Mohan Jain and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

In Vitro Embryogenesis in Higher Plants

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Author :
Publisher : Humana Press
ISBN 13 : 9781493979820
Total Pages : 558 pages
Book Rating : 4.9/5 (798 download)

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Book Synopsis In Vitro Embryogenesis in Higher Plants by : Maria Antonietta Germana

Download or read book In Vitro Embryogenesis in Higher Plants written by Maria Antonietta Germana and published by Humana Press. This book was released on 2019-03-29 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.