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Identification And Characterization Of Trypanosoma Brucei Ppr Proteins Putative Mitochondrial Rna Metabolism Proteins
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Book Synopsis Identification and Characterization of Trypanosoma Brucei PPR Proteins, Putative Mitochondrial RNA Metabolism Proteins by : Melissa Kay Mingler
Download or read book Identification and Characterization of Trypanosoma Brucei PPR Proteins, Putative Mitochondrial RNA Metabolism Proteins written by Melissa Kay Mingler and published by . This book was released on 2005 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification and Characterization of a Guide RNA-binding Protein from Trypanosoma Brucei Mitochondria by : Mark L. Hayman
Download or read book Identification and Characterization of a Guide RNA-binding Protein from Trypanosoma Brucei Mitochondria written by Mark L. Hayman and published by . This book was released on 2000 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of Pentatricopeptide Repeat (PPR) Proteins in Trypanosoma Brucei by :
Download or read book Characterization of Pentatricopeptide Repeat (PPR) Proteins in Trypanosoma Brucei written by and published by . This book was released on 2007 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pentatricopeptide repeat proteins (PPRs) are one of the largest newly identified protein families. PPR is characterized by the presence of a tandemly repeated 35 amino acids motif. It is extremely abundant in plants, and found in many organisms including mammals. PPRs are said to play essential roles in mitochondria, probably via binding to organelle transcripts. A number of recent reports characterizing PPR proteins indicate that PPR are important regulatory proteins that play essential roles in polycistronic RNA processing, RNA stability, and translation of special transcripts [2, 64]. To date, there are 23 PPRs identified in T.brucei by Mingler et al. [2] and 28 distinct PPRs detected by Pusnik et al. [1] by using different bioinformatics approaches. T.brucei encodes far more PPRs than the other non-plant organisms such as mammals. Trypanosomes undergoes a complex life cycle and thus it need to adapt to the changes of environment where it resides. T.brucei undergoes different morphological forms, metabolic changes, and alterations in gene expression during the changes of environment between insect and mammalian hosts. Thus, T.brucei serves as a good model to study the function of PPRs. We utilized tetracycline (tet)-regulated RNA interference (RNAi) to study the function of two T.brucei PPR proteins, TbPPR6 and TbPPR7. Northern blot analysis shows that TbPPR6 (Tb11.01.7930) was down-regulated by 49% while TbPPR7 (Tb927.3.4550) showed a decrease level of 23% in tetracycline induction RNAi cells. Knock-down of TbPPR6 caused a slow growth phenotype, while TbPPR7 knock-down caused only a minor slow growth phenotype, suggesting that TbPPR6 and TbPPR7 are essential for optimal growth of T.brucei . Poisoned primer extension assays demonstrated that depletion of TbPPR6 and TbPPR7 to the levels described above did not affect the mRNAs analyzed. However, there may be a modest effect on COI stability, particularly after depletion of TbPPR7. To characterize the RNA binding properties of TbPPR4 (Tb10.70.5780), recombinant TbPPR4 full length (PTbPR4-FL), and two of its truncated versions (TbPPR4-1, TbPPR4-0) were expressed and purified. Homopolymer binding assays show that TbPPR4-FL preferentially binds poly-(G) agarose beads over the other homopolymers. We further characterized the TbPPR4-RNA interaction by changing the salt concentration (NaCl) of the binding buffer. Increasing salt concentration in the binding buffer decreases the binding ability of TbPPR4-FL to poly-(G) agarose beads, indicating that electrostatic interactions play a prominent role in the binding of TbPPR4-FL. Interactions such as hydrogen bonding and hydrophobic interactions may also involve in the interactions of TbPPR4-FL with poly-(G). Titration experiments of TbPPR4-FL, TbPPR4-1, and TbPPR4-0 show that TbPPR4-FL binds significantly better than TbPPR4-1 and TbPPR4-0 while TbPPR4-1 binds better than TbPR4-0 to poly-(G). Thus, PPRs with increasing numbers of PPR motifs have higher affinity to RNA. Our studies add to the growing literature that defines PPR motifs as RNA binding motifs, and suggest that TbPPR6 and TbPPR7 maybe play a role in mitochondrial RNA processing and/or stability.
Download or read book Mitochondrial Gene Expression written by and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plant Mitochondria by : Olivier Van Aken
Download or read book Plant Mitochondria written by Olivier Van Aken and published by Humana. This book was released on 2021-10-15 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come.
Book Synopsis Molecular Biology and Biotechnology of Plant Organelles by : Henry Daniell, Ph.D.
Download or read book Molecular Biology and Biotechnology of Plant Organelles written by Henry Daniell, Ph.D. and published by Springer Science & Business Media. This book was released on 2007-11-04 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have taught plant molecular biology and biotechnology at the undergraduate and graduate level for over 20 years. In the past few decades, the field of plant organelle molecular biology and biotechnology has made immense strides. From the green revolution to golden rice, plant organelles have revolutionized agriculture. Given the exponential growth in research, the problem of finding appropriate textbooks for courses in plant biotechnology and molecular biology has become a major challenge. After years of handing out photocopies of various journal articles and reviews scattered through out the print and electronic media, a serendipitous meeting occurred at the 2002 IATPC World Congress held in Orlando, Florida. After my talk and evaluating several posters presented by investigators from my laboratory, Dr. Jacco Flipsen, Publishing Manager of Kluwer Publishers asked me whether I would consider editing a book on Plant Organelles. I accepted this challenge, after months of deliberations, primarily because I was unsuccessful in finding a text book in this area for many years. I signed the contract with Kluwer in March 2003 with a promise to deliver a camera-ready textbook on July 1, 2004. Given the short deadline and the complexity of the task, I quickly realized this task would need a co-editor. Dr. Christine Chase was the first scientist who came to my mind because of her expertise in plant mitochondria, and she readily agreed to work with me on this book.
Book Synopsis Genetics and Genomics of the Brassicaceae by : Renate Schmidt
Download or read book Genetics and Genomics of the Brassicaceae written by Renate Schmidt and published by Springer Science & Business Media. This book was released on 2010-12-03 with total page 675 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Genetics and Genomics of the Brassicaceae provides a review of this important family (commonly termed the mustard family, or Cruciferae). The family contains several cultivated species, including radish, rocket, watercress, wasabi and horseradish, in addition to the vegetable and oil crops of the Brassica genus. There are numerous further species with great potential for exploitation in 21st century agriculture, particularly as sources of bioactive chemicals. These opportunities are reviewed, in the context of the Brassicaceae in agriculture. More detailed descriptions are provided of the genetics of the cultivated Brassica crops, including both the species producing most of the brassica vegetable crops (B. rapa and B. oleracea) and the principal species producing oilseed crops (B. napus and B. juncea). The Brassicaceae also include important “model” plant species. Most prominent is Arabidopsis thaliana, the first plant species to have its genome sequenced. Natural genetic variation is reviewed for A. thaliana, as are the genetics of the closely related A. lyrata and of the genus Capsella. Self incompatibility is widespread in the Brassicaceae, and this subject is reviewed. Interest arising from both the commercial value of crop species of the Brassicaceae and the importance of Arabidopsis thaliana as a model species, has led to the development of numerous resources to support research. These are reviewed, including germplasm and genomic library resources, and resources for reverse genetics, metabolomics, bioinformatics and transformation. Molecular studies of the genomes of species of the Brassicaceae revealed extensive genome duplication, indicative of multiple polyploidy events during evolution. In some species, such as Brassica napus, there is evidence of multiple rounds of polyploidy during its relatively recent evolution, thus the Brassicaceae represent an excellent model system for the study of the impacts of polyploidy and the subsequent process of diploidisation, whereby the genome stabilises. Sequence-level characterization of the genomes of Arabidopsis thaliana and Brassica rapa are presented, along with summaries of comparative studies conducted at both linkage map and sequence level, and analysis of the structural and functional evolution of resynthesised polyploids, along with a description of the phylogeny and karyotype evolution of the Brassicaceae. Finally, some perspectives of the editors are presented. These focus upon the Brassicaceae species as models for studying genome evolution following polyploidy, the impact of advances in genome sequencing technology, prospects for future transcriptome analysis and upcoming model systems.
Book Synopsis Euglena: Biochemistry, Cell and Molecular Biology by : Steven D. Schwartzbach
Download or read book Euglena: Biochemistry, Cell and Molecular Biology written by Steven D. Schwartzbach and published by Springer. This book was released on 2017-04-19 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This much-needed book is the first definitive volume on Euglena in twenty-fire years, offering information on its atypical biochemistry, cell and molecular biology, and potential biotechnology applications. This volume gathers together contributions from well-known experts, who in many cases played major roles in elucidating the phenomenon discussed. Presented in three parts, the first section of this comprehensive book describes novel biochemical pathways which in some instances have an atypical subcellular localization. The second section details atypical cellular mechanisms of organelle protein import, organelle nuclear genome interdependence, gene regulation and expression that provides insights into the evolutionary origins of eukaryotic cells. The final section discusses how biotechnologists have capitalized on the novel cellular and biochemical features of Euglena to produce value added products. Euglena: Biochemistry, Cell and Molecular Biology will provide essential reading for cell and molecular biologists with interests in evolution, novel biochemical pathways, organelle biogenesis and algal biotechnology. Readers will come away from this volume with a full understanding of the complexities of the Euglena as well as new realizations regarding the diversity of cellular processes yet to be discovered.
Book Synopsis Somatic Genome Manipulation by : Xiu-Qing Li
Download or read book Somatic Genome Manipulation written by Xiu-Qing Li and published by Springer. This book was released on 2015-05-15 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Somatic genome manipulation is required when a sexual crossing approach cannot be used in breeding or genetic treatment of an individual organism. Examples can include gene- or cell-therapy of a person to correct disease, genetic improvement of vegetatively propagated plants, and genetic replacement of cytoplasm without significantly modifying the nuclear genome. The advantage of somatic genome manipulation is maintenance of the general genotype while correcting one or more traits. Somatic genome manipulation is also an option for genetic improvement of sexually propagated plants in polyploidy breeding or in overcoming issues of sexual incompatibility. Recent novel technologies in somatic genome manipulation are developing quickly but much of this literature is fragmented and difficult or inconvenient to access. This book represents the first attempt to assemble updated reviews, detailed protocols, and their applications in all fields in which somatic genome manipulation has thrived. This is a truely one-of-a-kind work that brings together the most important and relevant advances in somatic genome manipulation in plants, algae, microorganisms, humans and animals, and demonstrates where the science interacts and where it diverges. The chapters are written by experts on the topic with ready-to-use protocols that were originally developed or adapted from the literature in their laboratories. We expect that this book will be useful for students, researchers, and teachers in both plant and animal research as a resource for the latest information on somatic genome manipulation and for its useful laboratory methods.
Book Synopsis Protein Phosphatases and Stress Management in Plants by : Girdhar K. Pandey
Download or read book Protein Phosphatases and Stress Management in Plants written by Girdhar K. Pandey and published by Springer Nature. This book was released on 2020-09-26 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: The regulation of the phosphorylation/dephosphorylation process, resulting in “cellular switches” that monitor normal plant physiology, growth and development, has immense potential in crop systems. With much of the information in the nascent stages, coming largely from Arabidopsis and rice particularly, the use of cell biology, genetic screens, biochemical approaches aided by an omics approach should help unravel the detail functional information available about signaling pathways in plants. The regulation could be exploited to develop crop varieties better equipped to handle changing environments and enhance agricultural productivity. In the post-genomic era, one of the major challenges is investigation and understanding of multiple genes and gene families regulating a particular physiological and developmental aspect of plant life cycle. One of the important physiological processes is regulation of stress response, which leads to adaptation or adjustment in response to adverse stimuli. With the holistic understanding of the signaling pathways involving phosphatases, one gene family or multiple genes or gene families, plant biologist can lay a foundation for designing and generating future crops, which can withstand the higher degree of environmental stresses. Especially abiotic stresses, which are the major cause of crop loss throughout the world without losing crop yield and productivity. This book incorporates the contributions from leading plant biologists in the field of stress-mediated dephosphorylation by phosphatases as an important task to elucidate the aspects of stress signaling by functional genomic approaches.
Book Synopsis RNA Infrastructure and Networks by : Lesley J. Collins
Download or read book RNA Infrastructure and Networks written by Lesley J. Collins and published by Springer Science & Business Media. This book was released on 2011-09-15 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNAs form complexes with proteins and other RNAs. The RNA‐infrastructure represents the spatiotemporal interaction of these proteins and RNAs in a cell‐wide network. RNA Infrastructure and Networks brings together these ideas to illustrate the scope of RNA‐based biology, and how connecting RNA mechanisms is a powerful tool to investigate regulatory pathways. This book is but a taste of the wide range of RNA‐based mechanisms that connect in the RNA infrastructure.
Book Synopsis Biochemistry of Signal Transduction and Regulation by : Gerhard Krauss
Download or read book Biochemistry of Signal Transduction and Regulation written by Gerhard Krauss and published by John Wiley & Sons. This book was released on 2014-02-14 with total page 1024 pages. Available in PDF, EPUB and Kindle. Book excerpt: Originally based on a graduate course taught by the author, this true classic has once again been extensively updated to incorporate key new findings in biological signaling. With over half of the content re-written, plus 70 brand new and 50 revised figures, this is the most up-to-date textbook on signaling available anywhere. Thanks to its clear structure, hundreds of illustrative drawings, as well as chapter introductions and newly added study questions, this text excels as a companion for a course on biological signaling, and equally as an introductory reference to the field for students and researchers. Generations of students and junior researchers have relied on "the Krauss" to find their way through the bewildering complexity of biological signaling pathways.
Book Synopsis Evolutionary Biology—A Transdisciplinary Approach by : Pierre Pontarotti
Download or read book Evolutionary Biology—A Transdisciplinary Approach written by Pierre Pontarotti and published by Springer Nature. This book was released on 2020-10-29 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book includes 16 selected contributions presented at the 23rd Evolutionary Biology Meeting, which took place in Marseille in September 2019. The annual Evolutionary Biology Meetings in Marseille serve to gather leading evolutionary biologists and other scientists using evolutionary biology concepts, e.g. for medical research. The aim of these meetings is to promote the exchange of ideas to encourage interdisciplinary collaborations. Offering an up-to-date overview of recent findings in the field of evolutionary biology, this book is an invaluable source of information for scientists, teachers and advanced students.
Book Synopsis Translation in Mitochondria and Other Organelles by : Anne-Marie Duchêne
Download or read book Translation in Mitochondria and Other Organelles written by Anne-Marie Duchêne and published by Springer Science & Business Media. This book was released on 2013-09-13 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book gives an overview on the similarities and differences of the various translation systems. Moreover, it highlights the mechanisms and control of translation in mitochondria and other organelles such as chloroplasts, plastids and apicoplasts in different organisms. Lastly, it offers an outlook on future developments and applications that might be made possible by a better understanding of translation in mitochondria and other organelles.
Download or read book RNA Editing written by Ernesto Picardi and published by Humana. This book was released on 2021-08-13 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Genomics of Chloroplasts and Mitochondria by : Ralph Bock
Download or read book Genomics of Chloroplasts and Mitochondria written by Ralph Bock and published by Springer Science & Business Media. This book was released on 2012-06-05 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has witnessed an explosion of our knowledge on the structure, coding capacity and evolution of the genomes of the two DNA-containing cell organelles in plants: chloroplasts (plastids) and mitochondria. Comparative genomics analyses have provided new insights into the origin of organelles by endosymbioses and uncovered an enormous evolutionary dynamics of organellar genomes. In addition, they have greatly helped to clarify phylogenetic relationships, especially in algae and early land plants with limited morphological and anatomical diversity. This book, written by leading experts, summarizes our current knowledge about plastid and mitochondrial genomes in all major groups of algae and land plants. It also includes chapters on endosymbioses, plastid and mitochondrial mutants, gene expression profiling and methods for organelle transformation. The book is designed for students and researchers in plant molecular biology, taxonomy, biotechnology and evolutionary biology.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
