Antibody Engineering Methods And Protocols

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Antibody Engineering

Author : Damien Nevoltris
Publisher : Humana Press
ISBN 13 : 9781493986477
Total Pages : 543 pages
Book Rating : 4.9/5 (864 download)

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Book Synopsis Antibody Engineering by : Damien Nevoltris

Download or read book Antibody Engineering written by Damien Nevoltris and published by Humana Press. This book was released on 2018-09-24 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Antibody Engineering

Author : Benny K. C. Lo
Publisher : Springer Science & Business Media
ISBN 13 : 1592596665
Total Pages : 555 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Antibody Engineering by : Benny K. C. Lo

Download or read book Antibody Engineering written by Benny K. C. Lo and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Köhler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious—high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host’s immune s- tem for a therapeutic response. The initial excitement in finding Paul Ehrlich’s “magic bullet,” however, was met with widespread disappointment when it was demonstrated that murine antibodies frequently elicit the human anti-murine an- body (HAMA) response, thus rendering them ineffective and potentially unsafe in humans. Despite this setback, advances in recombinant DNA techniques over the last 15–20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA. The ability to p- duce bulk quantities of recombinant proteins from bacterial fermentation also fueled the design of numerous creative antibody constructs. To date, the United States Food and Drug Administration has approved more than 10 recombinant antibodies for human use, and hundreds more are in the development pipeline. The recent explosion in genomic and proteomic information appears ready to deliver many more disease targets amenable to antibody-based therapy.

Antibody Engineering

Author : Damien Nevoltris
Publisher : Humana
ISBN 13 : 9781493993536
Total Pages : 543 pages
Book Rating : 4.9/5 (935 download)

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Book Synopsis Antibody Engineering by : Damien Nevoltris

Download or read book Antibody Engineering written by Damien Nevoltris and published by Humana. This book was released on 2019-12-10 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Antibody Engineering

Author : Patrick Chames
Publisher : Humana Press
ISBN 13 : 9781493959440
Total Pages : 734 pages
Book Rating : 4.9/5 (594 download)

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Book Synopsis Antibody Engineering by : Patrick Chames

Download or read book Antibody Engineering written by Patrick Chames and published by Humana Press. This book was released on 2016-08-23 with total page 734 pages. Available in PDF, EPUB and Kindle. Book excerpt: More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an average rate of 50 per year and that is predicted to continue well into the future. Notwithstanding the many achievements already made in the field, there is still a lot of room for improvements for these molecules in terms of activity, and a plethora of approaches have been attempted to optimize these molecules. Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Antibody Engineering: Methods and Protocols, Second Edition serves as an invaluable resource for both experts and those new to the field, and most of all as a source of inspiration for the creation of the antibodies of tomorrow.

Antibody Engineering: Methods And Protocols

Author : Benny
Publisher :
ISBN 13 : 9788181288745
Total Pages : 576 pages
Book Rating : 4.2/5 (887 download)

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Book Synopsis Antibody Engineering: Methods And Protocols by : Benny

Download or read book Antibody Engineering: Methods And Protocols written by Benny and published by . This book was released on 2007-12-01 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Antibody Methods and Protocols

Author : Gabriele Proetzel
Publisher : Humana Press
ISBN 13 : 9781617799303
Total Pages : 325 pages
Book Rating : 4.7/5 (993 download)

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Book Synopsis Antibody Methods and Protocols by : Gabriele Proetzel

Download or read book Antibody Methods and Protocols written by Gabriele Proetzel and published by Humana Press. This book was released on 2012-06-24 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments. The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.

Antibody Engineering Protocols

Author : Sudhir Paul
Publisher : Springer Science & Business Media
ISBN 13 : 1592595383
Total Pages : 445 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Antibody Engineering Protocols by : Sudhir Paul

Download or read book Antibody Engineering Protocols written by Sudhir Paul and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols.

Single Domain Antibodies

Author : Dirk Saerens
Publisher : Humana Press
ISBN 13 : 9781617799679
Total Pages : 580 pages
Book Rating : 4.7/5 (996 download)

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Book Synopsis Single Domain Antibodies by : Dirk Saerens

Download or read book Single Domain Antibodies written by Dirk Saerens and published by Humana Press. This book was released on 2012-08-10 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Monoclonal Antibodies

Author : Maher Albitar
Publisher : Springer Science & Business Media
ISBN 13 : 1597453234
Total Pages : 472 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Monoclonal Antibodies by : Maher Albitar

Download or read book Monoclonal Antibodies written by Maher Albitar and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.

Introduction to Antibody Engineering

Author : Florian Rüker
Publisher : Springer Nature
ISBN 13 : 3030546306
Total Pages : 388 pages
Book Rating : 4.0/5 (35 download)

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Book Synopsis Introduction to Antibody Engineering by : Florian Rüker

Download or read book Introduction to Antibody Engineering written by Florian Rüker and published by Springer Nature. This book was released on 2021-01-04 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly readable textbook serves as a concise and engaging primer to the emerging field of antibody engineering and its various applications. It introduces readers to the basic science and molecular structure of antibodies, and explores how to characterize and engineer them. Readers will find an overview of the latest methods in antibody identification, improvement and biochemical engineering. Furthermore, alternative antibody formats and bispecific antibodies are discussed. The book’s content is based on lectures for the specializations “Protein Engineering” and “Medical Biotechnology” within the Master’s curriculum in “Biotechnology.” The lectures have been held at the University of Natural Resources and Life Sciences, Vienna, in cooperation with the Medical University of Vienna, since 2012 and are continuously adapted to reflect the latest developments in the field. The book addresses Master’s and PhD students in biotechnology, molecular biology and immunology, and all those who are interested in antibody engineering.

Antibody Methods and Protocols

Author : Gabriele Proetzel
Publisher : Humana Press
ISBN 13 : 9781493959303
Total Pages : 340 pages
Book Rating : 4.9/5 (593 download)

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Book Synopsis Antibody Methods and Protocols by : Gabriele Proetzel

Download or read book Antibody Methods and Protocols written by Gabriele Proetzel and published by Humana Press. This book was released on 2016-05-01 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology volume covers in vitro and in vivo generation of antibodies, as well as techniques for screening, analysis and modification of antibodies and antibody fragments. Offers materials lists, protocols and troubleshooting tips."

Methods in Molecular Biology: Antibody engineering protocols

Author : John M. Walker
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (84 download)

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Book Synopsis Methods in Molecular Biology: Antibody engineering protocols by : John M. Walker

Download or read book Methods in Molecular Biology: Antibody engineering protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein Engineering Protocols

Author : Kristian Müller
Publisher : Springer Science & Business Media
ISBN 13 : 1597451878
Total Pages : 318 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Protein Engineering Protocols by : Kristian Müller

Download or read book Protein Engineering Protocols written by Kristian Müller and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Synthetic Antibodies

Author : Thomas Tiller
Publisher : Humana
ISBN 13 : 9781493983193
Total Pages : 420 pages
Book Rating : 4.9/5 (831 download)

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Book Synopsis Synthetic Antibodies by : Thomas Tiller

Download or read book Synthetic Antibodies written by Thomas Tiller and published by Humana. This book was released on 2018-06-20 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.

Therapeutic Antibodies

Author : Antony S. Dimitrov
Publisher : Humana Press
ISBN 13 : 9781627038690
Total Pages : 0 pages
Book Rating : 4.0/5 (386 download)

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Book Synopsis Therapeutic Antibodies by : Antony S. Dimitrov

Download or read book Therapeutic Antibodies written by Antony S. Dimitrov and published by Humana Press. This book was released on 2014-11-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over 2000 years ago in China, antibodies elicited by early forms of vaccination likely played a major role in the protection of the population from infectious agents. Vac- nation has been further developed in Europe and described by Edward Jenner in the late-eighteenth century, then successfully implemented worldwide. The idea to use theactiveingredientinthebloodofvaccinated(orimmunized)animalsorhumansfor the treatment of diseases came a century later. It was made possible by a series of discoveries,suchastherealizationthattheserumfromanimalsimmunizedwithtoxins, for example, diphtheria toxin or viruses, is an effective therapeutic against the disease causedbythesameagentinhumans. Inthe1880s,vonBehringdevelopedanantitoxin (anti-body) that did not kill the bacteria but neutralized the bacterial toxin. The first Nobel Prize in Medicine (1901) was given to him for the discovery of the serum therapy. Acenturylater,22monoclonalantibodies(mAbs)areapprovedbytheUnited States Food and Drug Administration (FDA) for clinical use, and hundreds are in clinicaltrialsforthetreatmentofvariousdiseasesincludingcancers,immunedisorders, and infections. The revenues from the top-five therapeutic antibodies reached $11. 7 billion in 2006, and major pharmaceutical companies raced to acquire antibody biotech companies with a recent example of MedImmune, Inc. , which was acquired for $15. 6 billion by AstraZeneca in 2007. This explosion of research and development in the field of therapeutic antibodies prompted the publication of the MiMB volume Therapeutic Antibodies: Methods and Protocols. The book’s major goal is to present a set of protocols useful for researchers discoveringanddevelopingtherapeuticantibodies. Currentadvancesandfuturetrends in the antibody therapeutics are analyzed in the lead-in review article.

Antibody Engineering

Author : John McCafferty
Publisher : Oxford University Press on Demand
ISBN 13 : 9780199635924
Total Pages : 325 pages
Book Rating : 4.6/5 (359 download)

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Book Synopsis Antibody Engineering by : John McCafferty

Download or read book Antibody Engineering written by John McCafferty and published by Oxford University Press on Demand. This book was released on 1996 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researcher to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use. Topics in this volume include: generation and analysis of antibodies; antibody repertoires; antibody screening and selection; measuring antibody affinities; sequence analysis; antibody engineering and production; conversion of rodent antibodies to human antibodies by CDR grafting or guided selection; choosing and optimizing effector functions; preparation and use of antibody-based molecules in eukaryotic and prokaryotic systems; scaling up manufacture; and generation of high-affinity high-specificity human antibodies in appropriate formats. Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnostics, and therapeutics. Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success.

Recombinant Antibodies for Cancer Therapy

Author : Martin Welschof
Publisher : Springer Science & Business Media
ISBN 13 : 1592593348
Total Pages : 476 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Recombinant Antibodies for Cancer Therapy by : Martin Welschof

Download or read book Recombinant Antibodies for Cancer Therapy written by Martin Welschof and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the advent of hybridoma technology more than two decades ago, numerous antibodies have entered the clinical setting as potent therapeutic agents. Their repeated application in humans, however, is limited by the development of human antimouse antibodies (HAMA) in the recipient, leading to allergic re- tions against the foreign murine protein and rapid neutralization. To circumvent these limitations many new antibodies have recently been tailored through recombinant antibody technology. The initial clinical data show encouraging results, thus demonstrating the potential of these new therapeutic agents. The purpose of Recombinant Antibodies for Cancer Therapy is to present a collection of detailed protocols in recombinant antibody technology. It is pri- rily addressed to scientists working on recombinant antibodies as well as cli- cians involved with antibody-based therapies. As with other volumes of this series, we placed the main focus on providing detailed protocols describing procedures step-by-step. Moreover, each protocol supplies a troubleshooting guide containing detailed information on possible problems and hints for pot- tial solutions. Antibody technology is a subject of constant and rapid change. This volume, therefore, does not attempt to cover all possible current experimental approaches in the field. Rather, we present carefully selected protocols, written by competent authors who have successfully verified the particular method described. Given our own professional backgrounds and interest in oncology, we chose to conc- trate chiefly on therapeutic agents for cancer patients.