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Ultraviolet Spectroscopy Of Proteins
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Author :Alexander P. Demchenko Publisher :Springer Science & Business Media ISBN 13 :3642708471 Total Pages :323 pages Book Rating :4.6/5 (427 download)
Book Synopsis Ultraviolet Spectroscopy of Proteins by : Alexander P. Demchenko
Download or read book Ultraviolet Spectroscopy of Proteins written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.
Author :Donald M. Kirschenbaum Publisher :Springer Science & Business Media ISBN 13 :1468460870 Total Pages :298 pages Book Rating :4.4/5 (684 download)
Book Synopsis Atlas of Protein Spectra in the Ultraviolet and Visible Regions by : Donald M. Kirschenbaum
Download or read book Atlas of Protein Spectra in the Ultraviolet and Visible Regions written by Donald M. Kirschenbaum and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Once you have seen the spectrum of one protein you have seen the spectra of all pro teins. Or so it would seem. While the general characteristics of the absorption curve may appear to be similar for all proteins (i. e. , in acid and neutral solution there is a minimum at 250 nm, a maximum at 278-282 nm, and no absorption above 310 nm; in alkaline solution the maximum and minimum shift to longer wavelengths), there are subtle differences which can be seen when the spectra of many proteins are compared. It is these differences which reflect changes in amino acid content and in the milieu in which the protein has been dissolved. The spectra in this book provide samples of these subtle spectral differences and permit comparisons to be made. This book was prepared to have its index read and its contents referred to. For the reader who desires to know what a protein spectrum looks like in acid and alkaline media, after X-ray or UV irradiation, or after photo-oxidation or B-bromosuccinimide treatment, spectral representations of all these experimental situations and many others are available. The indicies were prepared to provide the maximum information with the minimum effort. In addition to an alphabetical listing, all spectra are referred to by species, tissues, and the organs from which they were taken. There are also "environmental" indicies related to the treatment the proteins received prior to having their spectra taken. Technical information concerning instrumentation is lacking.
Book Synopsis Atlas of Protein Spectra in the Ultraviolet and Visible Regions by : Donald M. Kirschenbaum
Download or read book Atlas of Protein Spectra in the Ultraviolet and Visible Regions written by Donald M. Kirschenbaum and published by Springer. This book was released on 1972-01-02 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Once you have seen the spectrum of one protein you have seen the spectra of all pro teins. Or so it would seem. While the general characteristics of the absorption curve may appear to be similar for all proteins (i. e. , in acid and neutral solution there is a minimum at 250 nm, a maximum at 278-282 nm, and no absorption above 310 nm; in alkaline solution the maximum and minimum shift to longer wavelengths), there are subtle differences which can be seen when the spectra of many proteins are compared. It is these differences which reflect changes in amino acid content and in the milieu in which the protein has been dissolved. The spectra in this book provide samples of these subtle spectral differences and permit comparisons to be made. This book was prepared to have its index read and its contents referred to. For the reader who desires to know what a protein spectrum looks like in acid and alkaline media, after X-ray or UV irradiation, or after photo-oxidation or B-bromosuccinimide treatment, spectral representations of all these experimental situations and many others are available. The indicies were prepared to provide the maximum information with the minimum effort. In addition to an alphabetical listing, all spectra are referred to by species, tissues, and the organs from which they were taken. There are also "environmental" indicies related to the treatment the proteins received prior to having their spectra taken. Technical information concerning instrumentation is lacking.
Book Synopsis Bibliographic Atlas of Protein Spectra in the Ultraviolet and Visible Regions by : Donald Kirschenbaum
Download or read book Bibliographic Atlas of Protein Spectra in the Ultraviolet and Visible Regions written by Donald Kirschenbaum and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz
Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2007-12-05 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
Book Synopsis Vibrational Spectroscopy in Protein Research by : Yukihiro Ozaki
Download or read book Vibrational Spectroscopy in Protein Research written by Yukihiro Ozaki and published by Academic Press. This book was released on 2020-05-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.
Book Synopsis Spectroscopy for the Biological Sciences by : Gordon G. Hammes
Download or read book Spectroscopy for the Biological Sciences written by Gordon G. Hammes and published by John Wiley & Sons. This book was released on 2005-08-19 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the physical principles of spectroscopy and their applications to the biological sciences Advances in such fields as proteomics and genomics place new demands on students and professionals to be able to apply quantitative concepts to the biological phenomena that they are studying. Spectroscopy for the Biological Sciences provides students and professionals with a working knowledge of the physical chemical aspects of spectroscopy, along with their applications to important biological problems. Designed as a companion to Professor Hammes's Thermodynamics and Kinetics for the Biological Sciences, this approachable yet thorough text covers the basic principles of spectroscopy, including: * Fundamentals of spectroscopy * Electronic spectra * Circular dichroism and optical rotary dispersion * Vibration in macromolecules (IR, Raman, etc.) * Magnetic resonance * X-ray crystallography * Mass spectrometry With a minimum of mathematics and a strong focus on applications to biology, this book will prepare current and future professionals to better understand the quantitative interpretation of biological phenomena and to utilize these tools in their work.
Book Synopsis Protein Fluorescence by : Joseph R. Lacowicz
Download or read book Protein Fluorescence written by Joseph R. Lacowicz and published by Springer. This book was released on 2013-11-20 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with each other by resonance energy transfer (RET) decreasing the tyrosine emission. In this sense a protein is similar to a three-particle or mul- particle problem in quantum mechanics where the interaction between particles precludes an exact description of the system. In comparison, it has been easier to interpret the fluorescence data from labeled proteins because the fluorophore density and locations could be controlled so the probes did not interact with each other. From the origins of biochemical fluorescence in the 1950s with Prof- sor G. Weber until the mid-1980s, intrinsic protein fluorescence was more qualitative than quantitative. An early report in 1976 by A. Grindvald and I. Z. Steinberg described protein intensity decays to be multi-exponential. Attempts to resolve these decays into the contributions of individual tryp- phan residues were mostly unsuccessful due to the difficulties in resolving closely spaced lifetimes.
Book Synopsis Circular Dichroism and Linear Dichroism by : Alison Rodger
Download or read book Circular Dichroism and Linear Dichroism written by Alison Rodger and published by Oxford University Press, USA. This book was released on 1997 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.
Book Synopsis Atlas of Protein Spectra in the Ultraviolet and Visible Regions by :
Download or read book Atlas of Protein Spectra in the Ultraviolet and Visible Regions written by and published by . This book was released on 1972 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Vibrational Spectroscopy in Protein Research by : Yukihiro Ozaki
Download or read book Vibrational Spectroscopy in Protein Research written by Yukihiro Ozaki and published by Academic Press. This book was released on 2020-05-19 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies. Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the field Emphasizes in vivo, in situ and non-invasive analysis of proteins in biomedical and life science research more broadly Contains chapters that address vibrational spectroscopy for the study of simple purified proteins and protein aggregates, fibrous proteins, membrane proteins and protein assemblies
Book Synopsis Methods for Structural Analysis of Protein Pharmaceuticals by : Wim Jiskoot
Download or read book Methods for Structural Analysis of Protein Pharmaceuticals written by Wim Jiskoot and published by Springer Science & Business Media. This book was released on 2005-12-05 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein pharmaceuticals form a fast-growing category in the arsenal of drugs. This book explores the nature of different analytical techniques and the way in which they are related to pharmaceutical proteins. In addition to serving the analytical chemist, this book is needed by the formulation scientist who is responsible for design and formulation of a pharmaceutical protein that can be monitored during production and over time.
Book Synopsis Protein'Ligand Interactions by : G. Ulrich Nienhaus
Download or read book Protein'Ligand Interactions written by G. Ulrich Nienhaus and published by Humana Press. This book was released on 2005-03-21 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Book Synopsis Therapeutic Peptides and Proteins by : Ajay K. Banga
Download or read book Therapeutic Peptides and Proteins written by Ajay K. Banga and published by CRC Press. This book was released on 2005-09-14 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: Upon publication of the first edition of Therapeutic Peptides and Proteins ten years ago there were only 19 biotechology medicines on the market. Currently there are more than 100, with at least 400 more in various stages of development. That alone would be grounds for a new edition. Add to that the fact that it is still difficult to find up
Book Synopsis Circular Dichroism and the Conformational Analysis of Biomolecules by : G.D. Fasman
Download or read book Circular Dichroism and the Conformational Analysis of Biomolecules written by G.D. Fasman and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 739 pages. Available in PDF, EPUB and Kindle. Book excerpt: ''Excellent and very timely....It will undoubtedly become a standard reference for the application of circular dichroism (CD) to biomolecules.'' --- Quarterly Review of Biology, March 1997 ''[T]estament to the book's utility is the fact that during the course of my review I had to 'rescue' it from the desks of graduate students on an almost daily basis. In summary, this is a great book.'' --- American Scientist ''Well documented chapters provide a very good insight into the problems surrounding the conformation of biomacromolecules...An indispensible source of information.'' --- Nahrung, 42(2), 1998 Renowned experts present the first state-of-the-art description of circular dichroism spectroscopy (CD). Chapters present in-depth discussions of the history of the field, the theory of CD for application to globular proteins, membrane proteins, peptides, nucleic acids and their interactions, carbohydrates, and instrumentation. Discussions also feature new techniques using synchrotron radiation, vibrational Raman optical activity, and vibrational CD. More than 250 illustrations supplement the text.
Book Synopsis Therapeutic Peptides by : Andrew E. Nixon
Download or read book Therapeutic Peptides written by Andrew E. Nixon and published by . This book was released on 2014 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.
Book Synopsis Analysis of Aggregates and Particles in Protein Pharmaceuticals by : Hanns-Christian Mahler
Download or read book Analysis of Aggregates and Particles in Protein Pharmaceuticals written by Hanns-Christian Mahler and published by John Wiley & Sons. This book was released on 2011-12-20 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes how to address the analysis of aggregates and particles in protein pharmaceuticals, provides a comprehensive overview of current methods and integrated approaches used to quantify and characterize aggregates and particles, and discusses regulatory requirements. Analytical methods covered in the book include separation, light scattering, microscopy, and spectroscopy.
