Tucannon River Spring Chinook Salmon Captive Brood Program, FY 2000 Annual Report

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Total Pages : 34 pages
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Book Synopsis Tucannon River Spring Chinook Salmon Captive Brood Program, FY 2000 Annual Report by :

Download or read book Tucannon River Spring Chinook Salmon Captive Brood Program, FY 2000 Annual Report written by and published by . This book was released on 2001 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes the objectives, tasks, and accomplishments of the Tucannon River spring chinook captive brood program from program inception (1997) through April 2001. The WDFW initiated a captive broodstock program in 1997. The overall goal of the Tucannon River captive broodstock program is for the short-term, and eventually long-term, rebuilding of the Tucannon River spring chinook salmon run, with the hope that natural production will eventually sustain itself. The project goal is to rear captive salmon to adults, spawn them, rear their progeny, and release approximately 150,000 smolts annually into the Tucannon River between 2003-2007. These smolt releases, in combination with the current hatchery supplementation program (132,000 smolts), and wild production, is expected to produce 600-700 returning adult spring chinook to the Tucannon River each year from 2005-2010. The Master Plan, Environmental Assessment, and most facility modifications at LFH were completed for the Tucannon River spring chinook captive broodstock program during FY2000 and FY2001. DNA samples collected since 1997 have been sent to the WDFW genetics lab in Olympia for baseline DNA analysis. Results from the genetic analysis are not available at this time. The captive broodstock program is planned to collect fish from five (1997-2001) brood years (BY). The captive broodstock program was initiated with 1997 BY juveniles, and the 2000 BY fish have been selected. As of April 30, 2001, WDFW has 172 BY 1997, 262 BY 1998, 407 BY 1999, and approximately 1,190 BY 2000 fish on hand at LFH. Twelve of 13 mature 97 BY females were spawned in 2000. Total eggtake was 14,813. Mean fecundity was 1,298 eggs/female based on 11 fully spawned females. Egg survival to eye-up was 47.3%. This low survival was expected for three year old captive broodstock females. As of April 30, 2001, WDFW has 4,211 captive broodstock progeny on hand. These fish will be tagged with blank wire tag without fin clips and released as smolts from Curl Lake Acclimation Pond into the Tucannon River during April 2002.

Tucannon River Spring Chinook Salmon Captive Broodstock Program, Annual Report 2001

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Total Pages : 42 pages
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Book Synopsis Tucannon River Spring Chinook Salmon Captive Broodstock Program, Annual Report 2001 by :

Download or read book Tucannon River Spring Chinook Salmon Captive Broodstock Program, Annual Report 2001 written by and published by . This book was released on 2002 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes the objectives, tasks, and accomplishments of the Tucannon River spring chinook captive brood during 2001. The WDFW initiated a captive broodstock program in 1997. The overall goal of the Tucannon River captive broodstock program is for the short-term, and eventually long-term, rebuilding of the Tucannon River spring chinook salmon run, with the hope that natural production will sustain itself. The project goal is to rear captive salmon selected from the supplementation program to adults, spawn them, rear their progeny, and release approximately 150,000 smolts annually into the Tucannon River between 2003-2007. These smolt releases, in combination with the current hatchery supplementation program (132,000 smolts) and wild production, are expected to produce 600-700 returning adult spring chinook to the Tucannon River each year from 2005-2010. The captive broodstock program will collect fish from five (1997-2001) brood years (BY). The captive broodstock program was initiated with 1997 BY juveniles, and the 2001 BY fish have been selected. As of Jan 1, 2002, WDFW has 17 BY 1997, 159 BY 1998, 316 BY 1999, 448 BY 2000, and approximately 1,200 BY 2001 fish on hand at LFH. The 2001 eggtake from the 1997 brood year (Age 4) was 233,894 eggs from 125 ripe females. Egg survival was 69%. Mean fecundity based on the 105 fully spawned females was 1,990 eggs/female. The 2001 eggtake from the 1998 brood year (Age 3) was 47,409 eggs from 41 ripe females. Egg survival was 81%. Mean fecundity based on the 39 fully spawned females was 1,160 eggs/female. The total 2001 eggtake from the captive brood program was 281,303 eggs. As of May 1, 2002 we have 171,495 BY 2001 captive brood progeny on hand. A total of 20,592 excess fish were marked as parr (AD/CWT) and will be released during early May, 2002 into the Tucannon River (rkm 40-45). This will allow us to stay within our maximum allowed number (150,000) of smolts released. During April 2002, WDFW volitionally released 3,055 BY 2000 captive broodstock progeny from Curl Lake Acclimation Pond into the Tucannon River. These fish were marked with agency-only wire tags and no fin clips in order to differentiate them from the supplementation fish (CWT/Right Red VI/No Finclip). Monitoring their survival and future releases to adult returns, along with future natural production levels, will determine the success or failure of this captive broodstock program.

Tucannon River Spring Chinook Salmon Hatchery Evaluation Program ... Annual Report

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Total Pages : 62 pages
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Download or read book Tucannon River Spring Chinook Salmon Hatchery Evaluation Program ... Annual Report written by and published by . This book was released on 2001 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tucannon River Spring Chinook Salmon Captive Broodstock Program, Annual Report 2002

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Total Pages : 53 pages
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Book Synopsis Tucannon River Spring Chinook Salmon Captive Broodstock Program, Annual Report 2002 by :

Download or read book Tucannon River Spring Chinook Salmon Captive Broodstock Program, Annual Report 2002 written by and published by . This book was released on 2003 with total page 53 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes the objectives, tasks, and accomplishments of the Tucannon River Spring Chinook Captive Broodstock Program during 2002. The WDFW initiated a captive broodstock program in 1997. The overall goal of the Tucannon River captive broodstock program is for the short-term, and eventually long-term, rebuilding of the Tucannon River spring chinook salmon run, with the hope that natural production will sustain itself. The project goal is to rear captive salmon selected from the supplementation program to adults, spawn them, rear their progeny, and release approximately 150,000 smolts annually into the Tucannon River between 2003-2007. These smolt releases, in combination with the current hatchery supplementation program (132,000 smolts) and wild production, are expected to produce 600-700 returning adult spring chinook to the Tucannon River each year from 2005-2010. The captive broodstock program collected fish from five (1997-2001) brood years (BY). As of January 1, 2003, WDFW has approximately 11 BY 1998, 194 BY 1999, 314 BY 2000, 447 BY 2001, and 300 BY 2002 (for extra males) fish on hand at LFH. The 2002 eggtake from the 1997 brood year (Age 5) was 13,176 eggs from 10 ripe females. Egg survival was 22%. Mean fecundity based on the 5 fully spawned females was 1,803 eggs/female. The 2002 eggtake from the 1998 brood year (Age 4) was 143,709 eggs from 93 ripe females. Egg survival was 29%. Mean fecundity based on the 81 fully spawned females was 1,650 eggs/female. The 2002 eggtake from the 1999 brood year (Age 3) was 19,659 eggs from 18 ripe females. Egg survival was 55%. Mean fecundity based on the 18 fully spawned fish was 1,092 eggs/female. The total 2002 eggtake from the captive brood program was 176,544 eggs. A total of 120,833 dead eggs (68%) were removed with 55,711 live eggs remaining for the program. As of May 1, 2003 we had 46,417 BY 2002 captive brood progeny on hand A total of 20,592 excess BY 01 fish were marked as parr (AD/CWT) and released during May 2002 into the Tucannon River (rkm 40-45). This allowed us to stay within our maximum allowed number (150,000) of smolts released. On August 20, 97 (21 1998 BY and 76 1999 BY) adult captive broodstock were determined to be in excess of eggtake goals and were outplanted into the Tucannon River at Panjab Bridge (rkm 74.5). Released fish were tagged with Monel jaw tags and radio transmitters were inserted into ten females for tracking and monitoring. Due to the low frequency of natural spawning by released fish, high mortality due to predation and illegal harvest, and high egg mortality in the hatchery during 2002, priority will be to release excess progeny as parr to stay within smolt release goals rather than release excess captive broodstock as adults. During April 2003, WDFW volitionally released 140,396 BY 2001 captive broodstock progeny smolts from Curl Lake Acclimation Pond into the Tucannon River. These fish were marked with agency-only wire tags and no fin clips in order to differentiate them from the supplementation fish (CWT/Right Red VIE/No Finclip). A total of 1,007 captive brood progeny smolts were PIT tagged to compare their outmigration with smolts from the supplementation program (1,010 tagged). Monitoring their survival and future releases to adult returns, along with future natural production levels, will determine the success or failure of this captive broodstock program.

Tucannon River Spring Chinook Salmon Hatchery Evaluation Program ... Annual Report

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Total Pages : 126 pages
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Download or read book Tucannon River Spring Chinook Salmon Hatchery Evaluation Program ... Annual Report written by and published by . This book was released on 2006 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tucannon River Spring Chinook Salmon Captive Broodstock Program

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ISBN 13 :
Total Pages : 58 pages
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Book Synopsis Tucannon River Spring Chinook Salmon Captive Broodstock Program by :

Download or read book Tucannon River Spring Chinook Salmon Captive Broodstock Program written by and published by . This book was released on 2009 with total page 58 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Program, 2000 Annual Report

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Total Pages : 61 pages
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Book Synopsis Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Program, 2000 Annual Report by : Richard W. Carmichael

Download or read book Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Program, 2000 Annual Report written by Richard W. Carmichael and published by . This book was released on 2003 with total page 61 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endangered Species Permit Number 1011 (formerly Permit No. 973) authorizes ODFW to take listed spring chinook salmon juveniles from Catherine Creek (CC), Lostine River (LR) and Grande Ronde River (GR) for research and enhancement purposes. Modification 2 of this permit authorizes ODFW to take adults for spawning and the production and release of smolts for the Captive and Conventional broodstock programs. This report satisfies the requirement that an annual report be submitted. Herein we report on activities conducted and provide cursory data analyses for the Grande Ronde spring chinook salmon Captive and Conventional broodstock projects from 1 January-31 December 2000.

Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Programs, 2003 Annual Report

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Total Pages : 22 pages
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Book Synopsis Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Programs, 2003 Annual Report by : Don Hair

Download or read book Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Programs, 2003 Annual Report written by Don Hair and published by . This book was released on 2004 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: BPA Fish and Wildlife Program Project Number 1998-01-001 provides funding for the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program. This report satisfies the requirement that an annual report be submitted for FY 2003. The Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Project is designed to rapidly increase numbers of salmon in stocks that are in imminent danger of extirpation. Parr are captured in Catherine Creek, upper Grande Ronde River and Lostine River and reared to adulthood in captivity. Upon maturation, these fish are spawned (within stocks) and their progeny reared to smoltification before being released into the natal stream of their parents. This program is co-managed by ODFW, National Marine Fisheries Service, Nez Perce Tribe and Confederated Tribes of the Umatilla Indian Reservation. This report covers activities conducted and provides data analyses for the Grande Ronde Spring Chinook Salmon Captive broodstock Program from 1 January--31 December 2003. Since the fiscal year ends in the middle of the spawning period, an annual report based on calendar year is more logical. This document is the FY 2003 annual report. Detailed information on historic and present population status, project background, goals and objectives, significance to regional programs and relationships to other programs, methods and previous results are available in the 1995-2002 Project Status Report (Hoffnagle et al 2003).

Tucannon River Spring Chinook Salmon Captive Brood Program

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Total Pages : 25 pages
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Book Synopsis Tucannon River Spring Chinook Salmon Captive Brood Program by : Joseph D. Bumgarner

Download or read book Tucannon River Spring Chinook Salmon Captive Brood Program written by Joseph D. Bumgarner and published by . This book was released on 2001 with total page 25 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tucannon River Spring Chinook Salmon Captive Broodstock Program

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ISBN 13 :
Total Pages : 33 pages
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Book Synopsis Tucannon River Spring Chinook Salmon Captive Broodstock Program by : Michael P. Gallinat

Download or read book Tucannon River Spring Chinook Salmon Captive Broodstock Program written by Michael P. Gallinat and published by . This book was released on 2002 with total page 33 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Snake River Spring/Summer Chinook Captive Broodstock Rearing and Research, 2003 Annual Report

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ISBN 13 :
Total Pages : 29 pages
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Book Synopsis Snake River Spring/Summer Chinook Captive Broodstock Rearing and Research, 2003 Annual Report by : W. Carlin McAuley

Download or read book Snake River Spring/Summer Chinook Captive Broodstock Rearing and Research, 2003 Annual Report written by W. Carlin McAuley and published by . This book was released on 2004 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1995, the National Marine Fisheries Service (NMFS), in cooperation with the Idaho Department of Fish and Game (IDFG), the Oregon Department of Fish and Wildlife (ODFW), and the Bonneville Power Administration (BPA) established captive broodstock programs to aid in the recovery of Snake River spring/summer chinook salmon (Oncorhynchus tshawytscha) listed as endangered under the U.S. Endangered Species Act (ESA). These programs are intended to provide safety nets for Salmon and Grande Ronde River Basins spring/summer chinook salmon stocks. They also provide a basis of examining the efficacy of captive rearing and captive breeding programs as tools for recovering listed salmonid populations. In years when no or few naturally produced fish return from the sea, captive fish and their progeny can be used to maintain populations in these two Snake River Basin tributaries. The NMFS facility at Manchester, WA provides the crucial seawater environment needed to culture anadromous salmonids during the marine phase of their life cycle. At the Manchester Research Station, the fish are cultured in 6.1m diameter circular tanks housed in a fully enclosed and secure building. The tanks are supplied with seawater that has been processed to eliminate most marine pathogens. The fish are fed a commercially prepared diet and held at densities and loading rates intended to maximize fish quality. When fish begin to mature, they are transferred to ODFW or IDFG freshwater facilities in Oregon and Idaho for final maturation. The states then release the mature fish (Idaho) or their progeny (Oregon) back into their native Snake River tributary waters in restoration efforts. In FY 2003, NMFS cultured 1998, 1999, 2000, and 2001 broodyear fish at its Manchester Facility. This report addresses program activities from September 1, 2002 to August 31, 2003.

Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Programs, 2002 Annual Report

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Total Pages : 40 pages
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Book Synopsis Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Programs, 2002 Annual Report by : Richard W. Carmichael

Download or read book Grande Ronde Basin Chinook Salmon Captive Brood and Conventional Supplementation Programs, 2002 Annual Report written by Richard W. Carmichael and published by . This book was released on 2003 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endangered Species Permit Number 1011 (formerly Permit No. 973) authorizes ODFW to take listed spring chinook salmon juveniles from Catherine Creek (CC), Lostine River (LR) and Grande Ronde River (GR) for research and enhancement purposes. Modification 2 of this permit authorizes ODFW to take adults for spawning and the production and release of smolts for the Captive and Conventional broodstock programs. This report satisfies the requirement that an annual report be submitted. Herein we report on activities conducted and provide cursory data analyses for the Grande Ronde spring chinook salmon Captive and Conventional broodstock projects from 1 January-31 December 2002. The Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Project is designed to rapidly increase numbers of salmon in stocks that are in imminent danger of extirpation. Parr are captured in Catherine Creek, upper Grande Ronde River and Lostine River and reared to adulthood in captivity. Upon maturation, they are spawned (within stocks) and their progeny reared to smoltification before being released into the natal stream of their parents. This program is co-managed by ODFW, National Marine Fisheries Service, the Nez Perce Tribe and Confederated Tribes of the Umatilla Indian Reservation.

Manchester Spring Chinook Broodstock Project

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Total Pages : 10 pages
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Book Synopsis Manchester Spring Chinook Broodstock Project by :

Download or read book Manchester Spring Chinook Broodstock Project written by and published by . This book was released on 2000 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: In spring 1995 the Idaho Department of Fish and Game (IDFG) and the Oregon Department of Fish and Wildlife (ODFW) initiated captive broodstocks as part of conservation efforts for ESA-listed stocks of Snake River spring/summer chinook salmon (Oncorhynchus tshawytscha). The need for this captive broodstock strategy was identified as critical in the National Marine Fisheries Service (NMFS) Proposed Recovery Plan for Snake River Salmon. These captive broodstock programs are being coordinated by the Bonneville Power Administration (BPA) through the Chinook Salmon Captive Propagation Technical Oversight Committee (CSCPTOC). Oregon's Snake River spring/summer chinook salmon captive broodstock program currently focuses on three stocks captured as juveniles from the Grande Ronde River Basin: the upper Grande Ronde River, Catherine Creek, and the Lostine River. Idaho's Snake River program includes three stocks captured as eggs and juveniles from the Salmon River Basin: the Lemhi River, East Fork Salmon River, and West Fork Yankee Fork. The majority of captive fish from each stock of the Grande Ronde Basin will be grown to maturity in freshwater at the ODFW Bonneville Hatchery. A minority of the Salmon River Basin stocks will be grown to maturity in freshwater at the IDFG Eagle Hatchery. However, the IDFG and ODFW requested that a portion of each group also be reared in protective culture in seawater. In August 1996, NMFS began a BPA funded project (Project 96-067-00) to rear Snake River spring/summer chinook salmon captive broodstocks in seawater at the NMFS Manchester Research Station. During 1997-1999, facilities modifications were undertaken at Manchester to provide secure facilities for rearing of these ESA-listed fish. This included construction of a building housing a total of twenty 6.1-m diameter fiberglass rearing tanks, upgrade of the Manchester salt water pumping and filtration/sterilization systems to a total capacity of 5,670 L/min (1,500 gpm), and installation of an ozone depuration system. Initial activities related to Project 96-067-00 are described in Flagg et al. (1997, 1998); related activities during the period 1998-1999 are described in McAuley et al. (2000). The current report summarizes NMFS activities on the Snake River spring/summer chinook salmon captive broodstock program at Manchester during FY 2000. In addition to husbandry activities, NMFS efforts also included participation in the CSCPTOC.

Summary Report for the 1997 Brood Year Methow Basin Spring Chinook Salmon Hatchery Program

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Total Pages : 64 pages
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Book Synopsis Summary Report for the 1997 Brood Year Methow Basin Spring Chinook Salmon Hatchery Program by : Heather R. Bartlett

Download or read book Summary Report for the 1997 Brood Year Methow Basin Spring Chinook Salmon Hatchery Program written by Heather R. Bartlett and published by . This book was released on 2001 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tucannon River Spring Chinook Captive Broodstock Program

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Total Pages : pages
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Book Synopsis Tucannon River Spring Chinook Captive Broodstock Program by :

Download or read book Tucannon River Spring Chinook Captive Broodstock Program written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Research on Captive Broodstock Programs for Pacific Salmon, 1999-2000 Annual Report

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ISBN 13 :
Total Pages : 124 pages
Book Rating : 4.:/5 (684 download)

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Book Synopsis Research on Captive Broodstock Programs for Pacific Salmon, 1999-2000 Annual Report by :

Download or read book Research on Captive Broodstock Programs for Pacific Salmon, 1999-2000 Annual Report written by and published by . This book was released on 2000 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program, 2008 Annual Report

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ISBN 13 :
Total Pages : 30 pages
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Book Synopsis Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program, 2008 Annual Report by :

Download or read book Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program, 2008 Annual Report written by and published by . This book was released on 2009 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program is designed to rapidly increase numbers of Chinook salmon in stocks that are in imminent danger of extirpation in Catherine Creek (CC), Lostine River (LR) and upper Grande Ronde River (GR). Natural parr are captured and reared to adulthood in captivity, spawned (within stocks) and their progeny reared to smoltification before being released into the natal stream of their parents. This program is co-managed by ODFW, National Marine Fisheries Service, Nez Perce Tribe and Confederated Tribes of the Umatilla Indian Reservation. Presmolt rearing was initially conducted at Lookingglass Fish Hatchery (LFH) but parr collected in 2003 and later were reared at Wallowa Fish Hatchery (WFH). Post-smolt rearing is conducted at Bonneville Fish Hatchery (BOH - freshwater) and at Manchester Research Station (MRS - saltwater). The CC and LR programs are being terminated, as these populations have achieved the goal of a consistent return of 150 naturally spawning adults, so the 2005 brood year was the last brood year collected for theses populations. The Grande Ronde River program continued with 300 fish collected each year. Currently, we are attempting to collect 150 natural parr and incorporate 150 parr collected as eggs from females with low ELISA levels from the upper Grande Ronde River Conventional Hatchery Program. This is part of a comparison of two methods of obtaining fish for a captive broodstock program: natural fish vs. those spawned in captivity. In August 2007, we collected 152 parr (BY 2006) from the upper Grande Ronde River and also have 155 Grande Ronde River parr (BY 2006) that were hatched from eyed eggs at LFH. During 2008, we were unable to collect natural parr from the upper Grande Ronde River. Therefore, we obtained 300 fish from low ELISA females from the upper Grande Ronde River Conventional Program. In October 2008 we obtained 170 eyed eggs from the upper Grande Ronde river Conventional Hatchery Program. We will attempt to collect natural parr in August 2009. This year 752 fish were removed from the captive population: 629 fish survived to gamete production and 123 fish died from various causes prior to spawning. Growth of the Captive Broodstock fish was similar to previous years. The saltwater fish have grown more slowly than those reared in freshwater. A total of 720 fish were sorted as maturing and 629 (87.4%) of them survived to spawn. We collected gametes from 273 females and 350 males from the 2002-2006 brood years in 2008, using 111 spawning matrices and collected 474,187 green eggs (1,737 eggs/female). All ripe males were spawned and no semen was collected for cryo-preservation. Of the 474,187 eggs collected for the BY 2008 F1 generation, 448,373 (94.6%) survived to the eyed stage. 68,612 (15.3%) were culled from females with high ELISA OD values for BKD prevention. For BY 2007, we collected a total of 477,048 eggs from all three populations and 407,369 (85.4%) reached the eyed stage, while 95,024 eyed eggs (23.3%) were culled for BKD prevention. Eyed eggs were hatched at Lookingglass Fish Hatchery, producing 267,131 fry. As parr, 153,371 fish were coded-wire tagged (CWT). For the 2006 F1 brood year, we collected 177,890 eggs and 149,073 (83.8%) reached the eyed stage. 83,826 eyed eggs (56.2%) were culled at the eyed stage for BKD prevention. 61,044 fry were produced (93.6%), 53,688 (88 %) survived to smolt. There were 54 bacterial kidney disease (BKD) mortalities at BOH and MRS, combined in this reporting period. Overall, there were fewer BKD mortalities in 2008 due to a reduced number of fish coming into the Captive Broodstock Program and a shift away from collecting wild parr to using eyed eggs from low ELISA females from the Conventional Hatchery Program. Unknown causes of death accounted for 32 deaths at MRS and BOH, combined in 2008. We continually examine and modify the operations of the Captive Broodstock Program to make improvements wherever possible. We continue to have difficulty with prevention and treatment of BKD outbreaks and continue to use erythromycin and azithromycin to treat this disease. We are also continuing to investigate other possible treatments and prophylactic measures. To reduce the incidence of BKD in offspring of the Captive Broodstock Program, we continue to allow culling of eyed eggs from females with high BKD ELISA values (generally>0.800 OD units but the cull level varies annually, depending on the distribution of ELISA values, number of eggs collected, and management considerations). We are also using ultrasound to determine maturity and sex of fish early in the maturation process and are now able to determine maturity and sex of most maturing fish in early April. This allows us to transfer maturing fish from saltwater to freshwater at a more natural time, which should improve fecundity and egg quality of saltwater-reared fish.