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Author : Douglas A. Hanggi
Publisher :
ISBN 13 :
Total Pages : 660 pages
Book Rating : 4.:/5 (319 download)
Download or read book High-performance Dye-ligand Affinity Chromatography Using Immobilized Triazine Dyes written by Douglas A. Hanggi and published by . This book was released on 1987 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : That T. Ngo
Publisher : Springer Science & Business Media
ISBN 13 : 1489918728
Total Pages : 560 pages
Book Rating : 4.4/5 (899 download)
Download or read book Molecular Interactions in Bioseparations written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Author : Elias Klein
Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 176 pages
Book Rating : 4.3/5 (91 download)
Download or read book Affinity Membranes written by Elias Klein and published by Wiley-Interscience. This book was released on 1991-02-14 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores the latest findings for both selective and efficient separation devices in the field of kidney research. It is divided into three major sections. Part one deals with the ``biochemistry'' part of the problem, including how to identify ligands of interest, how to link them to synthetic membranes, and some kinetic limitations of frontal elution chromatography. The second part comprehensively discusses the various substrata used in affinity separations and the formation processes of semi-permeable membranes. The final section explores the filtration processes using membranes and the kinetics of separations based on affinity membranes.
Author : Catherine Charcosset
Publisher : Elsevier
ISBN 13 : 0444563377
Total Pages : 349 pages
Book Rating : 4.4/5 (445 download)
Download or read book Membrane Processes in Biotechnology and Pharmaceutics written by Catherine Charcosset and published by Elsevier. This book was released on 2012-01-25 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane processes are increasingly used in pharmaceutical and biochemical engineering and biotechnology for concentration and purification, synthesis of molecules and drug delivery systems, and support for biochemical reactions. This book provides a state-of-the art overview of the classical membrane processes used in pharmaceutical and biochemical engineering and biotechnology, such as ultrafiltration, microfiltration, virus filtration, membrane chromatography, membrane emulsification, liquid membranes and membrane bioreactors. It describes the general rules (principles, choice of configurations, membranes, parameters, etc.), recent developments (fouling control, increase permeate flux and selectivity, etc.), applications, and theoretical descriptions. Further, it presents emerging processes such as solvent resistant nanofiltration and membrane crystallization. - Presents classical membrane processes such as ultrafiltration, microfiltration, virus filtration, membrane chromatography, membrane emulsification, liquid membranes and membrane bioreactors - Presents emerging processes such as solvent resistant nanofiltration and membrane crystallization - Gives a complete description of each technique (principles, membrane materials and devices, fouling control, and theoretical description) - Contains numerous examples of applications - Includes a uniform notation throughout the book enhancing the presentation and understanding of the content - Includes extensive list of references
Author :
Publisher :
ISBN 13 :
Total Pages : 1044 pages
Book Rating : 4.:/5 (31 download)
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1982 with total page 1044 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Elsevier
ISBN 13 : 0080858457
Total Pages : 395 pages
Book Rating : 4.0/5 (88 download)
Download or read book Principles, Materials and Techniques written by and published by Elsevier. This book was released on 1988-04-01 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles, Materials and Techniques
Author : Salvatore Fanali
Publisher : Elsevier
ISBN 13 : 0128093447
Total Pages : 840 pages
Book Rating : 4.1/5 (28 download)
Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-23 with total page 840 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Author : Jonathan L Sessler
Publisher : Royal Society of Chemistry
ISBN 13 : 1847552471
Total Pages : 431 pages
Book Rating : 4.8/5 (475 download)
Download or read book Anion Receptor Chemistry written by Jonathan L Sessler and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: Anion recognition plays a critical role in a range of biological processes, and a variety of receptors and carriers can be found throughout the natural world. Chemists working in the area of supramolecular chemistry have created a range of anion receptors, drawing inspiration from nature as well as their own inventive processes. This book traces the origins of anion recognition chemistry as a unique sub-field in supramolecular chemistry while illustrating the basic approaches currently being used to effect receptor design. The combination of biological overview and summary of current synthetic approaches provides a coverage that is both comprehensive and comprehensible. First, the authors detail the key design motifs that have been used to generate synthetic receptors and which are likely to provide the basis for further developments. They also highlight briefly some of the features that are present in naturally occurring anion recognition and transport systems and summarise the applications of anion recognition chemistry. Providing as it does a detailed review for practitioners in the field and a concise introduction to the topic for newcomers, Anion Receptor Chemistry reflects the current state of the art. Fully referenced and illustrated in colour, it is a welcome addition to the literature.
Author : Bengi Uslu
Publisher : Elsevier
ISBN 13 : 0128232404
Total Pages : 478 pages
Book Rating : 4.1/5 (282 download)
Download or read book Electroanalytical Applications of Quantum Dot-Based Biosensors written by Bengi Uslu and published by Elsevier. This book was released on 2021-05-19 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantum dots (QDs) are hybrid organic/inorganic nanoparticles with novel physical properties. QDs have two components: an inorganic core and an optically active coated shell. Moreover, surface coatings can be applied to QDs to modify the particle as needed for experiments. Hydrophilic coatings prevent leaking of metal cargo from the core, enhancing the solubility in biological contexts and bind molecules, such as receptor–ligands, antibodies, therapeutic, and diagnostic macromolecules for enhanced effects. Their high surface-to-volume ratio allows multiple functional groups to attach onto the surface of the particles at constant surface volume. Silicon-, gallium-, indium-, or germanium-based; cadmium-based; and carbon-based QDs have already been used in many applications, such as imaging probes for the engineering of multifunctional nanodevices. Superior properties of QDs make them an excellent system in technology and biotechnology.This book describes electroanalytical applications of QD-based nanobiosensors, including brief information about the synthesis and characterization of QDs and basics of electroanalytical methods, followed by QDs in electrochemical biomimetic sensors, QDs in microchips, inorganic materials doped QDs, QD-based electrochemical DNA biosensors, electroluminescence for biomarker analysis using aptamer-based QDs, QD-based photoelectrochemical techniques, enzyme-based nanobiosensors using QDs, QD-based electrochemical immunosensors, and QD-modified nanosensors in drug analysis. - Outlines QD-based applications for drug, food, clinical, and environmental science - Shows how the properties of QDs make them effective ingredients in biosensing applications - Assesses the major challenges in integrating QDs in biosensing systems
Author : Mahesh Uttamchandani
Publisher : Methods in Molecular Biology
ISBN 13 : 9781493982424
Total Pages : 274 pages
Book Rating : 4.9/5 (824 download)
Download or read book Small Molecule Microarrays: Methods and Protocols written by Mahesh Uttamchandani and published by Methods in Molecular Biology. This book was released on 2018-12-05 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ashok Pandey
Publisher : Elsevier
ISBN 13 : 0444636730
Total Pages : 888 pages
Book Rating : 4.4/5 (446 download)
Download or read book Current Developments in Biotechnology and Bioengineering written by Ashok Pandey and published by Elsevier. This book was released on 2016-09-17 with total page 888 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for isolating and purifying them from a production medium. During the last few years, the tools of molecular biology and genetic and metabolic engineering have rendered tremendous improvements in the production of industrial products by fermentation. Structured by industrial product classifications, this book provides an overview of the current practice, status, and future potential for the production of these agents, along with reviews of the industrial scenario relating to their production. - Provides information on industrial bioprocesses for the production of microbial products by fermentation - Includes separation and purification processes of fermentation products - Presents economic and feasibility assessments of the various processes and their scaling up - Links biotechnology and bioengineering for industrial process development
Author : Richard R Burgess
Publisher : Academic Press
ISBN 13 : 0080923178
Total Pages : 915 pages
Book Rating : 4.0/5 (89 download)
Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Author : Jan-Christer Janson
Publisher : John Wiley & Sons
ISBN 13 : 0471746614
Total Pages : 556 pages
Book Rating : 4.4/5 (717 download)
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 2011-03-22 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography
Author : Hongzhang Chen
Publisher : Academic Press
ISBN 13 : 0128025948
Total Pages : 292 pages
Book Rating : 4.1/5 (28 download)
Download or read book Technologies for Biochemical Conversion of Biomass written by Hongzhang Chen and published by Academic Press. This book was released on 2016-12-14 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technologies for Biochemical Conversion of Biomass introduces biomass biochemical conversion technology, including the pretreatment platform, enzyme platform, cell refining platform, sugar platform, fermentation platform, and post-treatment platform. Readers will find a systematic treatment, not only of the basics of biomass biochemical conversion and the introduction of each strategy, but also of the current advances of research in this area. Researchers will find the key problems in each technology platform for biomass biochemical conversion identified and solutions offered. This valuable reference book features new scientific research and the related industrial application of biomass biochemical conversion technology as the main content, and then systematically introduces the basic principles and applications of biomass biochemical conversion technology. - Combines descriptions of these technologies to provide strategies and a platform for biochemical conversion in terms of basic knowledge, research advances, and key problems - Summarizes models of biomass biochemical conversion for multiple products - Presents products of biomass biochemical conversion from C1 to C10
Author : Robert A. Goodnow, Jr.
Publisher : John Wiley & Sons
ISBN 13 : 1118487680
Total Pages : 495 pages
Book Rating : 4.1/5 (184 download)
Download or read book A Handbook for DNA-Encoded Chemistry written by Robert A. Goodnow, Jr. and published by John Wiley & Sons. This book was released on 2014-04-28 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively describes the development and practice of DNA-encoded library synthesis technology. Together, the chapters detail an approach to drug discovery that offers an attractive addition to the portfolio of existing hit generation technologies such as high-throughput screening, structure-based drug discovery and fragment-based screening. The book: Provides a valuable guide for understanding and applying DNA-encoded combinatorial chemistry Helps chemists generate and screen novel chemical libraries of large size and quality Bridges interdisciplinary areas of DNA-encoded combinatorial chemistry – synthetic and analytical chemistry, molecular biology, informatics, and biochemistry Shows medicinal and pharmaceutical chemists how to efficiently broaden available "chemical space" for drug discovery Provides expert and up-to-date summary of reported literature for DNA-encoded and DNA-directed chemistry technology and methods
Author : Paul Cutler
Publisher : Springer Science & Business Media
ISBN 13 : 159259655X
Total Pages : 474 pages
Book Rating : 4.5/5 (925 download)
Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Author : Thomas E. Crowley
Publisher : Academic Press
ISBN 13 : 0124095933
Total Pages : 267 pages
Book Rating : 4.1/5 (24 download)
Download or read book Experiments in the Purification and Characterization of Enzymes written by Thomas E. Crowley and published by Academic Press. This book was released on 2014-01-11 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Includes advanced analytical techniques - Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH - Includes access to a website with additional resources for instructors
