The Microbial Community Associated with the Florida Red Tide Dinoflagellate Karenia Brevis

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ISBN 13 :
Total Pages : 296 pages
Book Rating : 4.:/5 (711 download)

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Book Synopsis The Microbial Community Associated with the Florida Red Tide Dinoflagellate Karenia Brevis by : Patricia Blair Roth

Download or read book The Microbial Community Associated with the Florida Red Tide Dinoflagellate Karenia Brevis written by Patricia Blair Roth and published by . This book was released on 2005 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Investigating Algicidal Amines as Agents for Chemical Control of Toxic Dinoflagellate Karenia Brevis

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ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.3/5 (83 download)

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Book Synopsis Investigating Algicidal Amines as Agents for Chemical Control of Toxic Dinoflagellate Karenia Brevis by : Gretchen Johnson

Download or read book Investigating Algicidal Amines as Agents for Chemical Control of Toxic Dinoflagellate Karenia Brevis written by Gretchen Johnson and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The dinoflagellate Karenia brevis causes harmful algal blooms, called red tides, that increasingly disrupt ecosystems and economic activity in the western coast of Florida. Strategizing for a future of imminent red tide demands interventive control options for severe blooms. Bio-inspired chemical control attempts to synergistically merge the concepts of chemical control and biotic control, incorporating the logistical advantages of the former and the environmental consciousness of the latter. Amine compounds identified in a dinoflagellate-specific bacterial algicide were assessed for their potential to control the abundance of K. brevis. First, dose-response of K. brevis to four selected compounds was measured. Three of those, ammonia, putrescine, and spermidine, controlled K. brevis and had lesser effects on a nontarget algal species. To improve dose efficiency, combinations of these compounds were screened for synergistic interaction. Three combined amine doses potentiated by synergisms proceeded to testing on natural microbial communities collected from a field site subject to red tide. The impact of these doses on nontarget microbes was compared. 0.9 ppm ammonium with 7.5 ppm putrescine, hereafter "NP", emerged as preferable with respect to effects on photosynthetic biomass and photophysiological stress. Next, NP was applied to a naturally-occurring red tide where it controlled K. brevis and restructured microbial communities. Dynamics in microbial communities and water chemistry parameters were then monitored over 7 days in NP-treated. NP demonstrated control of non-K. brevis dinoflagellates in non-red tide communities, with specificity over diatoms and other phytoplankton groups. Effects of NP on pH, turbidity, and dissolved oxygen of mesocosms revealed pertinent considerations for future field application. Putrescine had short residence times whereas ammonia accumulated to potentially toxic levels. Ultimately, NP, formulated from commercially-available and relatively inexpensive amine compounds produced by naturally-occurring algicidal bacteria, exhibited potent, taxonomically-specific control of K. brevis in complex natural microbial communities. Thus, efforts to replicate this result and further engineer NP for eventual field deployment are supported.

Ecology of Harmful Algae

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Publisher : Springer Science & Business Media
ISBN 13 : 9783540740094
Total Pages : 448 pages
Book Rating : 4.7/5 (4 download)

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Book Synopsis Ecology of Harmful Algae by : E. Granéli

Download or read book Ecology of Harmful Algae written by E. Granéli and published by Springer Science & Business Media. This book was released on 2007-10-04 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harmful algal can cause a variety of deleterious effects, including the poisoning of fish and shellfish, habitat disruptions for many organisms, water discoloration, beach fouling, and even toxic effects for humans. In this volume, international experts provide an in-depth analysis of harmful algae topics and offer a comprehensive synthesis of the latest research in the field.

Zooplankton Population Dynamics in Relation to the Red Tide Dinoflagellate Karenia Brevis on the West Florida Shelf of the Gulf of Mexico

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (833 download)

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Book Synopsis Zooplankton Population Dynamics in Relation to the Red Tide Dinoflagellate Karenia Brevis on the West Florida Shelf of the Gulf of Mexico by : Brianne M. Walsh

Download or read book Zooplankton Population Dynamics in Relation to the Red Tide Dinoflagellate Karenia Brevis on the West Florida Shelf of the Gulf of Mexico written by Brianne M. Walsh and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Characterization and Regulation of Gene Expression Networks in Response to Acute Environmental Stress in the Florida Red Tide Dinoflagellate, Karenia Brevis

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ISBN 13 :
Total Pages : 498 pages
Book Rating : 4.:/5 (228 download)

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Book Synopsis Characterization and Regulation of Gene Expression Networks in Response to Acute Environmental Stress in the Florida Red Tide Dinoflagellate, Karenia Brevis by : Kristy Blair Lidie

Download or read book Characterization and Regulation of Gene Expression Networks in Response to Acute Environmental Stress in the Florida Red Tide Dinoflagellate, Karenia Brevis written by Kristy Blair Lidie and published by . This book was released on 2007 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Characterization of Polyketide Synthases in the Florida Red Tide Dinoflagellate Karenia Brevis

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ISBN 13 :
Total Pages : 410 pages
Book Rating : 4.:/5 (456 download)

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Book Synopsis Characterization of Polyketide Synthases in the Florida Red Tide Dinoflagellate Karenia Brevis by : Emily A. Monroe

Download or read book Characterization of Polyketide Synthases in the Florida Red Tide Dinoflagellate Karenia Brevis written by Emily A. Monroe and published by . This book was released on 2008 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biochemical Analysis of Diel Vertical Migration in the Red Tide Dinoflagellate Karenia Brevis

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (656 download)

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Book Synopsis Biochemical Analysis of Diel Vertical Migration in the Red Tide Dinoflagellate Karenia Brevis by :

Download or read book Biochemical Analysis of Diel Vertical Migration in the Red Tide Dinoflagellate Karenia Brevis written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Many laboratories have solely used the Wilson isolate to characterize the red tide dinoflagellate species Karenia brevis. Recent work has provided new isolates from different geographical locations. Prior to detailed biochemical investigations, laboratory studies were conducted on ten different isolates representing geographical locations around the coast of Florida. The investigation of the physiological parameters for the ten different isolates provided a foundation for the proper selection of a single isolate that would closely represent the species in the field. Each isolate was capable of a range of responses with primary dependence on cell counts within the cultures. Evidence suggests that either carbon limitation or bacterial negative feedback caused changes in the physiological parameters as cell counts increased. The Apalachicola isolate was selected for further biochemical investigations because it was representative of the group response. A nutrient-replete intermediate-light mesocosm experiment investigated the taxis patterns, and the biochemical composition of vertically migrating K. brevis populations. This experiment confirmed the internal biochemical status of the cell controlled migratory behavior, growth and reproduction. Young cells fixed carbon at the surface while older cells limited vertical migration toward the middle depth in anticipation of cellular division. The cells were capable of performing complex lipid control during vertical migration. Quick turnover of these lipids indicated K. brevis was more than capable of taking advantage of changing physical conditions. Finally, nutrient replete and deplete mesocosms investigated how K. brevis responded to field-simulated high light conditions. Results from high light and nitrogen-limited exposure suggested K. brevis may utilize the benthos by minimizing exposure to oxidative stress and as a nitrogen source supplied from benthic pore-water flow in permeable sediments. High light also caused K.

Lipidomics

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Publisher : Springer Nature
ISBN 13 : 1071629662
Total Pages : 375 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Lipidomics by : Sanjoy K. Bhattacharya

Download or read book Lipidomics written by Sanjoy K. Bhattacharya and published by Springer Nature. This book was released on 2023-01-18 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.

Cell Cycle Regulation in the Florida Red Tide Dinoflagellate, Karenia Brevis

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ISBN 13 :
Total Pages : 446 pages
Book Rating : 4.:/5 (829 download)

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Book Synopsis Cell Cycle Regulation in the Florida Red Tide Dinoflagellate, Karenia Brevis by : Stephanie Alexandra Brunelle

Download or read book Cell Cycle Regulation in the Florida Red Tide Dinoflagellate, Karenia Brevis written by Stephanie Alexandra Brunelle and published by . This book was released on 2011 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Effects of the Red Tide Producing Dinoflagellate, Karenia Brevis, and Associated Brevetoxins on Viability and Sublethal Stress Responses in Scleractinian Coral

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (16 download)

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Book Synopsis The Effects of the Red Tide Producing Dinoflagellate, Karenia Brevis, and Associated Brevetoxins on Viability and Sublethal Stress Responses in Scleractinian Coral by : David Anthony Reynolds

Download or read book The Effects of the Red Tide Producing Dinoflagellate, Karenia Brevis, and Associated Brevetoxins on Viability and Sublethal Stress Responses in Scleractinian Coral written by David Anthony Reynolds and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Coral cover is in decline on a global scale, with increased mortality events being attributed to a number of global and regional stressors. While the impacts of global stressors (e.g. sea surface temperature rise, ocean acidification) are well documented, there is growing interest in identifying and understanding the impacts of regional stressors. The reason for this change in focus is that regional stressors can often work in combination, sometimes synergistically, with global stressors and that stressors on a regional scale tend to be more easily mitigated by management practices. One regional stressor that impacts a myriad of marine organisms in the southeastern United States is the annual red tide blooms produced by the dinoflagellate, Karenia brevis. Their impacts, along with the lipid soluble polyether neurotoxins they produce, termed brevetoxins, are well studied in economically important organisms, such as bivalves. However, little is known of their impacts on organisms that possess ecological importance, such as species of scleractinian coral. To address this gap in knowledge, a multifaceted study is discussed herein, which evaluated the effects of ecologically relevant concentrations of K. brevis and associated brevetoxins on different coral life history stages and coral species. The second chapter addresses the impacts of red tide on larval behavior, settlement and survival of the coral species Porites astreoides, as well as impacts of photochemical efficiency and oxidative stress within different coral species (P. astreoides larvae, P. astreoides adults, Acropora cervicornis, Cladocora arbuscula, and Phyllangia americana). The third chapter confers the use of broad-scale proteomic analysis to identify the cellular response of the non-model coral species, P. astreoides, following exposure to red tide. Coral larvae actively avoided both medium and high bloom conditions of K. brevis and brevetoxins, while percent larval settlement and survival were impacted following exposure to high bloom concentrations of K. brevis. Photochemical efficiency of in hospite Symbiodinium was reduced following exposure to both K. brevis and brevetoxin in P. astreoides larvae, as well as exposure to K. brevis in P. astreoides adults, while being unimpacted in A. cervicornis. Compared to controls, high bloom conditions resulted in an increase in biomarkers of lipid peroxidation in C. arbuscula. This was also seen in P. astreoides larvae at 24 hours; however, this difference was indistinguishable following 48 hours. Surprisingly, no other biomarker of oxidative stress analyzed were impacted. Broad-scale proteomic analysis of P. astreoides following exposure to red tide conditions revealed variable changes in proteome expression depending on if the corals were exposed to K. brevis or brevetoxins. Exposure to brevetoxins resulted in differential expression of proteins related to DNA organization, chromatin formation and transcription expression; while exposure to K. brevis resulted in differential expression of proteins related to redox homeostasis, protein folding, energy metabolism, and production of reactive oxygen species. The results of this study demonstrate the potential for annual red tide blooms to act as a regional stressor on coral species. They highlight the ability of red tide conditions to negatively impact coral at multiple life history stages and that the extent of these effects may be species specific. They also provide further incite of coral's response to red tide exposure at the cellular level.

Grazing on Synnechococcis Spp. by the Red-tide Dinoflagellate Karenia Brevis

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ISBN 13 :
Total Pages : 296 pages
Book Rating : 4.:/5 (857 download)

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Book Synopsis Grazing on Synnechococcis Spp. by the Red-tide Dinoflagellate Karenia Brevis by : Leo A. Procise

Download or read book Grazing on Synnechococcis Spp. by the Red-tide Dinoflagellate Karenia Brevis written by Leo A. Procise and published by . This book was released on 2012 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Karenia Brevis Hot Spots in the West Florida Shelf and Their Associated Socio-economic Implications

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (12 download)

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Book Synopsis Karenia Brevis Hot Spots in the West Florida Shelf and Their Associated Socio-economic Implications by : Diana A Moanga

Download or read book Karenia Brevis Hot Spots in the West Florida Shelf and Their Associated Socio-economic Implications written by Diana A Moanga and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Harmful algal blooms are almost an annual occurrence on the West Florida Shelf. They are caused by the toxic dinoflagellate Karenia brevis (K. brevis). Intense bloom events result in significant environmental, economic, and human-health impacts associated with the release of potent natural toxins, known as brevotoxins. If inhaled or ingested they can produce substantial adverse health effects. In order to mitigate and minimize the vast array of impacts associated with K. brevis blooms, an overarching interdisciplinary framework is needed. A critical factor in understanding K. brevis dynamics requires mapping and monitoring bloom development and transport, and identifying areas of localized bloom maxima, also referred to as bloom hot spots. To date, no studies have identified the spatial location and extent of clusters of statistically significant hot spots in Florida coastal waters. Few existing studies provide any confidence level when identifying areas characterized as hot spots. The goals of this research was to accurately identify K. brevis hot spot areas during different bloom periods, and explore potential correlations between school absenteeism rates and the distance from toxic bloom hot spots. Additionally, a coastal vulnerability index was developed in an attempt to assess the likelihood that some regions will experience greater health impacts from aerosolized brevotoxin exposure, compared to others. This was done through the use of Geographic Information System (GIS). The GIS Hot Spot Analysis function identified areas of significant clusters of K. brevis, while spatial interpolation methods illustrated a visual display of the extent and intensity of recurring coastal blooms.

In Vitro Search for Quorum Sensing Activity in Batch Cultures of Harmful Dinoflagellates and Algicidal Bacteria and Improved Bioassay Methods for Applications in Marine Matrices

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ISBN 13 :
Total Pages : 147 pages
Book Rating : 4.:/5 (869 download)

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Book Synopsis In Vitro Search for Quorum Sensing Activity in Batch Cultures of Harmful Dinoflagellates and Algicidal Bacteria and Improved Bioassay Methods for Applications in Marine Matrices by : Walter Marshall Blair

Download or read book In Vitro Search for Quorum Sensing Activity in Batch Cultures of Harmful Dinoflagellates and Algicidal Bacteria and Improved Bioassay Methods for Applications in Marine Matrices written by Walter Marshall Blair and published by . This book was released on 2013 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of harmful algal blooms (HABs) that impact coastal zones worldwide is regulated by a wide range of biophysical and chemical factors. How the complex microbial community associated with HABs coordinates density-dependent activities may be related to bloom dynamics and toxin production, and is a rapidly growing research area. Quorum sensing (QS) systems allow some bacteria and eukaryotes to synchronize density-dependent behaviors such as biofilm formation, virulence factor expression, and bioluminescence. The Autoinducer-1 (AI-1) and Autoinducer-2 (AI-2) QS systems in particular are shared by a wide variety of bacterial taxa and can be influenced by a growing number of eukaryotes. Involvement of the Florida red tide dinoflagellates, Karenia spp., and their associated bacteria (including algicidal taxa) in QS systems would introduce a new paradigm for understanding biotic regulation of bloom dynamics. The potential roles of AI-1 and AI-2 systems in cultures of dinoflagellates and algicidal bacteria were investigated using bioassays based on Chromobacterium violaceum and Vibrio harveyi, respectively. The present study demonstrated a lack AI-1 production with a detection limit of 500 nM for oxo-C10-HSL and a lack of AI-2 production that was confirmed by LC-MS/MS analysis with a detection limit of 47 nM. The LC-MS/MS analysis did confirm low nanomolar baseline levels of AI-2 in samples and medium blanks that were likely due to carry over from the natural seawater-based culture media. Nonetheless, the present study improved upon the standard bioassay protocol for screening QS production, eliminating potential false positives and also identifying severe limitations in the AI-1 and AI-2 inhibition format. The negative experimental results as well as the methodological improvements and cautions reported here will be valuable in guiding future HAB-related QS research.

Biochemical Analysis of Diel Vertical Migration in the Red Tide Dinoflagellate Karenia Brevis

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ISBN 13 : 9780549077756
Total Pages : 188 pages
Book Rating : 4.0/5 (777 download)

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Book Synopsis Biochemical Analysis of Diel Vertical Migration in the Red Tide Dinoflagellate Karenia Brevis by : Blake Allen Schaeffer

Download or read book Biochemical Analysis of Diel Vertical Migration in the Red Tide Dinoflagellate Karenia Brevis written by Blake Allen Schaeffer and published by . This book was released on 2006 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: A nutrient-replete intermediate-light mesocosm experiment investigated the taxis patterns, and the biochemical composition of vertically migrating K. brevis populations. This experiment confirmed the internal biochemical status of the cell controlled migratory behavior, growth and reproduction. Young cells fixed carbon at the surface while older cells limited vertical migration toward the middle depth in anticipation of cellular division. The cells were capable of performing complex lipid control during vertical migration. Quick turnover of these lipids indicated K. brevis was more than capable of taking advantage of changing physical conditions.

Influence of Water Column Stratification and Nutrient Gradients On The Migratory Behavior of The Red Tide Dinoflagellate, Karenia Brevis

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (953 download)

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Book Synopsis Influence of Water Column Stratification and Nutrient Gradients On The Migratory Behavior of The Red Tide Dinoflagellate, Karenia Brevis by : Matthew Garrett

Download or read book Influence of Water Column Stratification and Nutrient Gradients On The Migratory Behavior of The Red Tide Dinoflagellate, Karenia Brevis written by Matthew Garrett and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Diel vertical migration (DVM) can offer motile phytoplankton a competitive advantage over other phytoplankton species. DVM has been well documented in the harmful dinoflagellate, Karenia brevis; however, the extent to which changes in nutrient regimes and density stratification modify migration patterns has not been thoroughly investigated. A 2 m experimental column was constructed with sensors and sampling ports at four depths to obtain fluorescence and temperature measurements and discrete water samples. The environmental parameters of the experimental column represented temperature and nutrient conditions found on the West Florida Shelf (WFS), where Karenia blooms frequently occur. Results demonstrated a clear DVM pattern for K. brevis where surface aggregations occurred during light periods, and bottom aggregations occurred in dark periods. However, the rate and intensity of migration varied between experimental conditions. Injections of nutrient replete water at the surface and bottom did not appear to modify migratory behavior, in that a portion of the population resided at depth regardless of light or dark periods. Weak density stratification caused a delay in downward migration in dark periods, whereas strong density stratification caused a complete cessation of downward migration. In a weakly stratified environment with a bottom injected replete water mass, DVM patterns most closely replicate the pattern under weakly stratified conditions alone. Modifications in the natural DVM pattern of K. brevis with respect to varying environmental and nutrient conditions that occur on the WFS can have major implications current forecast model predictions, and for monitoring and mitigation strategies.

Linkages Between Biochemical Fluxes and Migration Behavior in Populations of the Red Tide Dinoflatgellate [sic], Karenia Brevis

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ISBN 13 :
Total Pages : 294 pages
Book Rating : 4.:/5 (646 download)

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Book Synopsis Linkages Between Biochemical Fluxes and Migration Behavior in Populations of the Red Tide Dinoflatgellate [sic], Karenia Brevis by : Xuemei Bai

Download or read book Linkages Between Biochemical Fluxes and Migration Behavior in Populations of the Red Tide Dinoflatgellate [sic], Karenia Brevis written by Xuemei Bai and published by . This book was released on 2004 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

A Study of the Interactions Between Algicidal Bacteria and the Toxic Dinoflagellate Karenia Brevis Using Molecular Techniques

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ISBN 13 :
Total Pages : 208 pages
Book Rating : 4.:/5 (485 download)

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Book Synopsis A Study of the Interactions Between Algicidal Bacteria and the Toxic Dinoflagellate Karenia Brevis Using Molecular Techniques by : Xavier Mayali

Download or read book A Study of the Interactions Between Algicidal Bacteria and the Toxic Dinoflagellate Karenia Brevis Using Molecular Techniques written by Xavier Mayali and published by . This book was released on 2001 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: