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The Discovery Validation And Fine Mapping Of Stripe Rust Resistance Qtl In A Hexaploid Wheat Mapping Population
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Book Synopsis The Discovery, Validation, and Fine Mapping of Stripe Rust Resistance QTL in a Hexaploid Wheat Mapping Population by : Iago Zebediah Lowe
Download or read book The Discovery, Validation, and Fine Mapping of Stripe Rust Resistance QTL in a Hexaploid Wheat Mapping Population written by Iago Zebediah Lowe and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The bane of wheat-based agricultural societies for millennia, the rust diseases continue to pose the single largest biotic threat to global wheat production despite more than a century of systematic scientific work on this important pathosystem. Recent breaches of numerous widely deployed sources of resistance have brought the wheat rusts once more to the center of global agricultural concerns and urge us to consider how, aided by new tools in molecular genetics and genomics, we may one day develop truly durable strategies of resistance to these sophisticated and evolving pathogens. Chapter 1 provides an historical overview of wheat rust resistance and argues that a new conceptual framework, one prioritizing the functional classification of quantitative resistance genes, is needed if the objective of developing deployable strategies of durable rust resistance is to be met. Chapter 2 details the identification and validation of stripe rust resistance QTL segregating in a hexaploid wheat mapping population. By focusing on sources of partial resistance, phenotyping under pressure from a broadly-virulent pathogen population in the field, characterizing the mapping population over multiple seasons, and validating the discovered QTL in independent populations, this study models the approach to rust resistance QTL identification and validation outlined in the first chapter. Chapter 3 presents the reasons for prioritizing continued investment in Yr48, a non-major resistance gene identified in the described mapping population. This discussion is followed by further phenotypic characterization of Yr48 and dissection of its validated QTL region, work which sets the stage for the construction of a physical map of the Yr48 region and the positional cloning of this novel source of resistance. The final section of this dissertation outlines the likely future research activities motivated by the results presented in the previous chapters. While it may be several years before Yr48 is cloned, this dissertation provides a solid justification and foundation for that work. And through its eventual identification and functional characterization, Yr48 promises to advance our understanding of the mechanisms of quantitative resistance to wheat stripe rust and thereby facilitate the long-term goal of engineering strategies of durable resistance to this historic agricultural pathogen.
Book Synopsis Discovery and Fine Mapping of a Locus for Adult Plant Stripe Rust Resistance in Hexaploid Wheat by : Nicolas Cobo
Download or read book Discovery and Fine Mapping of a Locus for Adult Plant Stripe Rust Resistance in Hexaploid Wheat written by Nicolas Cobo and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Cereal rusts are among the most destructive plant diseases, and have affected human food supply for millennia. Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is currently the most important of the three wheat rusts in California. Since the year 2000, the appearance of newer, more virulent and aggressive Pst races with tolerance to high temperatures has been associated with devastating epidemics worldwide and prompted the search for new sources of Pst resistance. In Chapter 1, four Pst resistance QTL effective against the new Pst races were identified using recombinant inbred lines (RILs) developed from a cross between the Argentinean varieties Klein Proteo and Klein Chajá. These QTL showed additive effects and they confer adequate levels of field resistance when combined. However, only QYr.ucw-1BL showed effective adult-plant resistance (APR) during the six years it was tested (P
Book Synopsis Molecular Mapping of Rust Resistant Qtls in a Synthetic Hexaploid Wheat Population by : Jeffrey David Kovach
Download or read book Molecular Mapping of Rust Resistant Qtls in a Synthetic Hexaploid Wheat Population written by Jeffrey David Kovach and published by . This book was released on 2017 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stripe Rust written by Xianming Chen and published by Springer. This book was released on 2017-07-11 with total page 723 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively introduces stripe rust disease, its development and its integral control. Covering the biology, genetics, genome, and functional genomics of the pathogen, it also discusses host and non-host resistance, their interactions and the epidemiology of the disease. It is intended for scientists, postgraduates and undergraduate studying stripe rust, plant pathology, crop breeding, crop protection and agricultural science, but is also a valuable reference book for consultants and administrators in agricultural businesses and education.
Book Synopsis Precise Mapping of Hessian Fly and Stripe Rust Resistance Loci in Pacific Northwest Wheat Germplasm (Triticum Aestivum L.) by : Esra Alwan
Download or read book Precise Mapping of Hessian Fly and Stripe Rust Resistance Loci in Pacific Northwest Wheat Germplasm (Triticum Aestivum L.) written by Esra Alwan and published by . This book was released on 2019 with total page 183 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hessian fly and stripe rust are major pests of spring wheat in the Pacific Northwest and cultivar resistance is a primary breeding goal. Hessian fly (HF) infestations continue to cause significant yield losses in spring wheat in the Pacific Northwest. In wheat, resistance to Hessian fly is usually controlled in a gene for gene manner, similar to other pathosystems. Hessian fly resistance genes in wheat have been failing as a result to the rapid evolutionary pace of the insect. Stripe rust (denoted by Yr) is a destructive foliar disease of wheat casing damage on an annual basis. Most of the Yr named resistance genes have been overcome as a result of the continuing emergence of new virulence races. Genetic resistance remains the most effective and economical approach to minimize yield losses and respond to pathogen evolution. A doubled haploid (DH) mapping population was generated from a cross between two elite spring wheat lines; 'WA8076', and 'HT080158LU'. The DH population was genotyped with 90K SNP markers using the Illumina Infinium platform. Phenotypic assessment was carried out on the DH population for both Hessian fly and stripe rust response. The genotyping efforts resulted in a total 15,236 polymorphic SNP markers used to establish a high-density genetic map. A single Hessian fly resistance gene (HFR) derived from 'WA8076' was detected on the distal region of chromosome 6BS, flanked by two SNP markers IWB71431 and IWB61175 at a distance of 2.6 cM. In addition, a total of 13 genes/QTL were found to be associated with stripe rust resistance. The adapted superior lines with Yr resistance will be directly incorporated in subsequent breeding efforts as a durable source of resistance. The closely linked SNP markers to the resistance loci, identified in this study, will provide an effective tool to accurately identify, select, and integrate the HF and Yr resistance genes into existing wheat cultivars. The highly dense SNP-based genetic map provides useful information for fine mapping and finding candidate genes underlying QTLs as well as marker-assisted breeding. Furthermore, a genome-wide association study (GWAS) we performed for both seedling and adult plant response in a diverse population of winter wheat germplasm. The population was genotyped with the 90K iSelect wheat single nucleotide polymorphism (SNP) array. Our GWAS study identified a total of 26 loci, comprising a range of existing and novel loci associated with stripe rust resistance. Resistance loci in the winter wheat germplasm can be exploited in the development of broadly-effective disease-resistant commercial wheat cultivars.
Book Synopsis QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population by : Carmen Johanne Barkley
Download or read book QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population written by Carmen Johanne Barkley and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat (Triticum spp.) is a crop of major economic and dietary importance. In the Pacific Northwest region of the United States, soft white wheat is the primary class of wheat grown and is an important export product for the region. Soft white wheat flour is especially important in producing products such as cookies, crackers, pancakes, noodles and pastry products. The goal of this project was to map quantitative trait loci (QTL) for end-use quality and stripe rust (Puccinia striiformis f. sp. tritici) resistance in a double haploid mapping population derived from a cross between a soft white winter club wheat, ‘Cara’, and a soft white winter lax wheat, ‘Xerpha’. The mapping population of 126 individuals was grown across 12 environments from 2013 to 2017 and rated for stripe rust resistance. In a separate experiment, it was grown in 16 different locations from 2013 to 2016 and phenotyped for 16 end-use quality traits. A linkage map of 23 linkage groups was constructed for the population using 1282 genotyping-by-sequencing (GBS) and KASP markers. QTL analysis revealed 35 significant QTL for stripe rust resistance. Two of the most interesting markers in the stripe rust resistance analysis were IWB38687 and A2927, which were each associated with three separate QTL. Analysis of the end-use quality trait data identified 22 significant QTL. Some of the most significant QTL identified for end-use quality traits had LOD scores well over five and explained over 15% of the variance in the trait they were associated with. Some of the markers identified in this study could be good targets for marker assisted selection in PNW wheat breeding programs, especially because the parents of our mapping population are commonly used in breeding programs in the PNW.
Book Synopsis QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) by : Emily Frances Klarquist
Download or read book QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) written by Emily Frances Klarquist and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fine Mapping of a Stripe Rust Resistance Gene Transferred from Triticum Monococcum L. Into Hexaploid Wheat Triticum Aestivum L. by : Simarjit Kaur
Download or read book Fine Mapping of a Stripe Rust Resistance Gene Transferred from Triticum Monococcum L. Into Hexaploid Wheat Triticum Aestivum L. written by Simarjit Kaur and published by . This book was released on 2008 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fine-mapping of the Major Resistance QTL Lr34 in Hexaploid Wheat and Physical Characterization of Its Target Region in Ae. Tauschii by : Liselotte Selter
Download or read book Fine-mapping of the Major Resistance QTL Lr34 in Hexaploid Wheat and Physical Characterization of Its Target Region in Ae. Tauschii written by Liselotte Selter and published by . This book was released on 2007 with total page 79 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis QTL Mapping of Stem Rust Resistance Loci in Durum Wheat Populations by : Shitaye Homma Megerssa
Download or read book QTL Mapping of Stem Rust Resistance Loci in Durum Wheat Populations written by Shitaye Homma Megerssa and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem rust caused by Puccinia graminis f. sp. tritici Eriks. & Henn is the most destructive disease of durum and common wheat. The main focus of this study is to identify loci associated with stem rust resistance in durum wheat using association mapping and linkage mapping. A panel of 283 lines and 224 recombinant inbred lines (RILs) from a cross between 'Reichenbachii' and 'DAKIYE' developed by the durum wheat breeding program of the International Maize and Wheat Improvement Center (CIMMYT) were used for the study. The panel was evaluated against races TTKSK, TKTTF, JRCQC and TTRTF at the seedling stage and TKTTF and JRCQC in the field in Ethiopia from 2018 to 2019 for two seasons. The same panel was evaluated against bulk of multiple stem rust races prevalent in Ethiopia and Kenya from 2018 to 2019 in five environments. Genome-wide association study (GWAS) was conducted using 26,439 single nucleotide polymorphism (SNP) markers for seedling response (280 lines) and field response (283 lines) to stem rust. The RILs along with the two parents were evaluated for response to bulk of multiple stem rust races in Ethiopia and Kenya for two seasons from 2019 to 2020. Linkage analyses were conducted using 843 SNP markers for 175 lines. For GWAS of seedling response, a mixed linear model (MLM) identified 17 quantitative trait loci (QTL) of which eight were putatively novel while FarmCPU identified 20 QTL and 12 were likely novel. For field resistance to races TKTTF and JRCQC, MLM detected 19 QTL of which 12 were likely novel while FarmCPU detected 16 QTL and seven were putatively novel. For resistance to multiple Pgt races in East Africa, 160 significant marker-trait associations (MTAs) grouped into 42 QTL were identified using MLM and FarmCPU and 21 QTL were likely novel. From previously reported Sr genes, the regions of Sr7a, Sr8a, Sr8155B1, Sr11, Sr12, alleles of Sr13, Sr17, Sr22/Sr25, and Sr49 were identified. For the biparental population, composite interval mapping (CIM) identified three QTL on chromosomes 3B (QSr.cnl-3B), 4B (QSr.cnl-4B) and 7B (QSr.cnl-7B). These three QTL contributed by the resistant parent explained 4.7% to 15.3% of the phenotypic variation and all match previously reported loci. Lines with multiple-race stem rust resistance can be used as parents in durum wheat resistance breeding to stem rust and markers identified in the GWAS can be used in marker-assisted selection (MAS) once validated in a different population. Further study on the validation of allele specific markers and allelism tests in the Sr13 region of chromosome 6A is needed. Future evaluation of large numbers of durum wheat lines and searching for durable adult plant resistance gene is crucial in resistance breeding of durum wheat.
Book Synopsis Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest by : Lu Liu
Download or read book Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest written by Lu Liu and published by . This book was released on 2018 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), is one of the most devastating and economically important diseases across major wheat growing regions in the world. Growing resistant cultivars is the most effective and environmentally sound method for controlling the disease. This study was conducted to identify resistance genes/QTL in three winter wheat cultivars Madsen, Skiles and Eltan and develop molecular markers for marker-assisted selection. Madsen has shown high-level and durable resistance since its release in 1988 and was identified to have both all-stage resistance (ASR) and high-temperature adult-plant (HTAP) resistance. Two major effect ASR QTL on chromosome arms 1AS and 2AS, one minor ASR QTL on 1BS and two HTAP resistance QTL on 3BS and 6BS were identified in Madsen. Skiles is susceptible to all tested Pst races at seedling stage but shows strong resistance at adult-plant stage with higher resistance level expressed at high temperature, indicating that Skiles has only HTAP resistance against the tested Pst races. Six HTAP resistance QTL were mapped in Skiles, with two major QTL mapped on 3BS and 4BL and four minor QTL on 1BL, 5AL, 6B and 7DL. Markers linked to the QTL were used to select highly resistant breeding lines derived from crosses with Skiles. Eltan has shown variable reactions from moderate susceptibility to resistance in different years since its release in 1990. Five QTL were detected in Eltan, with two major ASR QTL on 2BS and 4BL, one minor ASR on 5BS and two minor HTAP resistance QTL on 6AS and 7BL. The two major ASR QTL became less effective due to the fact that the Pst population has changed to virulent, leading to the resistance reduction in Eltan. By studying these three wheat cultivars with different genes for different types of resistance, it is concluded that the best strategy to achieve high-level and durable resistance is to combine several genes for effective ASR and adequate HTAP resistance. These studies provide the information of useful genes and molecular markers for improving resistance to stripe rust in wheat cultivars.
Book Synopsis Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population by : Wardah K. Mustahsan
Download or read book Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population written by Wardah K. Mustahsan and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat is a vital cereal crop, providing 20 % of the daily nutritional requirements for consumers worldwide. Although there has been a substantial increase in production and yield gains, food demands will still be on the rise in future decades, and wheat yields must continue to increase rapidly to meet these demands. Grain yield can be inhibited by biotic factors (pathogens), which require the development of resistant varieties against pathogens. Stripe (yellow) rust produces massive yield losses in wheat production, and ample resistance against stripe rust is achieved by pyramiding multiple resistant genes together. Yield also can be improved by characterizing the underlying mechanisms that define yield and yield-related traits. We used breeding technology tools to dissect the genetic architecture of yield-related traits, yield, and stripe rust resistance to identify genomic regions that can improve these traits in wheat germplasm and broaden the genetic resources available for breeders to develop robust breeding germplasm that can improve the profitability and resilience of wheat production. Stripe (yellow) rust, caused by Puccinia striiformis (Pst), is a devastating disease of wheat worldwide. In commercial production, stripe rust reduces forage yield, grain yield and grain quality. Yield losses caused by stripe rust range from 10% to 70%. This study was conducted using two different wheat reference genomes (IWGSC v2.1('Chinese Spring') and 'Jagger') to identify quantitative trait loci (QTL) associated with adult plant field resistance to stripe rust in the hard winter wheat RIL population, 'Overley' x 'Overland' to provide breeders with identified genomic regions associated with quantitative field resistance. Our QTL analysis identified genomic regions on 2AS (2N[superscript v]S translocation), 2BS, 2BL, and 2DL using two different mapping methods (Inclusive Composite Interval Mapping and Multi-Environmental Trial Analysis) that were associated with stripe rust resistance for both infection type and severity using genetic maps from both genomes. There were no meaningful differences in interpreting the data from the two maps. Overley contributed the resistance alleles at the 2AS and 2BL QTLs. Overland contributed the resistance alleles at the 2BS and 2DL QTLs. The 2DL QTL (QYr.hwwg-2D) will be further refined due its environmental stability for resistance. We designed PCR-based SNP marker assays to efficiently identify these genomic regions in breeding populations. Yield improvement can be facilitated by a deeper understanding of the underlying infrastructure of components that determine grain yield. We performed a QTL analysis on the population Overley x Overland, to identify genomic regions in our population can explain the variation in yield and its components in field trials using the two different reference maps. We identified major QTLs in both reference maps for yield (3BL), single kernel weight (4AL), plant height (6AS), grains per spike (4AL), thousand grain weight (4AL), and physiological maturity (2BS). The Jagger map identified more QTLs (40 QTLs) than the IWGSCv2.1 map (30 QTLs). The future direction of these studies will be marker development to utilize these QTLs in yield-improvement breeding programs.
Book Synopsis Molecular Mapping of Stripe Rust Resistance and Freezing Tolerance in Winter Wheat (Triticum Aestivum L.) by : Austin Joel Case
Download or read book Molecular Mapping of Stripe Rust Resistance and Freezing Tolerance in Winter Wheat (Triticum Aestivum L.) written by Austin Joel Case and published by . This book was released on 2012 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis QTL Mapping of Pre-harvest Sprouting and Stripe Rust Resistance in Wheat Cultivars Danby and Tiger by : Mingqin Shao
Download or read book QTL Mapping of Pre-harvest Sprouting and Stripe Rust Resistance in Wheat Cultivars Danby and Tiger written by Mingqin Shao and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat yield and quality is influenced by many abiotic and biotic environmental factors. Pre-harvest sprouting (PHS) occurs when physiologically matured spikes are exposed to wet field conditions before harvest, which results in seed germination and causes significant losses in yield and end-use quality. Wheat stripe rust is one of the most important biotic factors reducing grain yield and quality. To investigate the genetic basis of the resistance to PHS and stripe rust in hard white winter wheat cultivars Danby and Tiger and develop molecular markers for marker- assisted breeding, a double haploid (DH) population, derived from those two cultivars, was genotyped with simple sequence repeats (SSR) markers and simple nucleotide polymorphism (SNP) markers. This DH population was assessed for resistance to PHS and stripe rust in both greenhouse and field experiments. For PHS, one major resistant quantitative trait locus (QTL) was consistently detected on the short arm of chromosome 3A in all three experiments conducted and explained 21.6% to 41.0% of the phenotypic variation (PVE). This QTL is corresponding to a previously cloned gene, TaPHS1. A SNP in the promoter of TaPHS1 co- segregated with PHS resistance in this mapping population. Meanwhile, two other QTLs, Qphs.hwwg-3B.1 and Qphs.hwwg-5A.1, were consistently detected on the chromosome arms 3BS and 5AL in two experiments. These two QTLs showed significant additive effects with TaPHS1 in improving PHS resistance. For stripe rust, three major QTLs were consistently detected in four out of six environments for infection type (IT) or disease severity (DS). Two of them, QYr.hwwg-2AS1 and QYr.hwwg-4BL1, contributed by the Danby allele explained up to 28.4% of PVE for IT and 60.5% of PVE for DS. The third QTL, QYr.hwwg-3BS1, contributed by the Tiger allele, had PVE values up to 14.7% for IT and 22.9% for DS. QYr.hwwg-2AS1 and QYr.hwwg- 4BL1 are likely the same resistance genes reported previously on chromosome arms 2AS and 4BL. However, QYr.hwwg-3BS1 might be different from the reported gene cluster near the distal end of 3BS where Yr57, Yr4, Yr30 and Sr2 were located. Significant additive effects on reducing IT and DS were observed among these three major QTLs. In order to pyramid multiple QTLs in breeding, user-friendly Kompetitive allele specific PCR (KASP) markers were successfully developed for several QTLs identified in this study. The QTLs and their interactions found in this study together with those novel flanking KASP markers developed will be useful not only for understanding genetic mechanisms of PHS and stripe rust resistance but also for marker- assisted breeding to improve wheat resistance to PHS and stripe rust by gene pyramiding.
Book Synopsis Development of Fine-mapping Resources for Interrogation of a Yield Impacting Qtl on the 2d Chromosome in a Bread Wheat (triticum Aestivum) and Aegilops Tauschii Nested Association Mapping Population by : Jonathan Dubau Turkus
Download or read book Development of Fine-mapping Resources for Interrogation of a Yield Impacting Qtl on the 2d Chromosome in a Bread Wheat (triticum Aestivum) and Aegilops Tauschii Nested Association Mapping Population written by Jonathan Dubau Turkus and published by . This book was released on 2021 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bread wheat (Triticum aestivum) is one of the dominant grain crops across the world. As a mainstay of diets across the world, there is a constant pressure by the breeders and geneticists to identify yield promoting loci. In previous work, significant SNP associations were found on the 2D chromosome (approximately at 23.5 Mb and 25.2 Mb) for yield variation in a genome-wide association study (GWAS) of the D-genome Nested Association Mapping population (DNAM), an advanced-backcross nested association mapping population of five Ae. tauschii lines into an elite bread wheat background. To identify the signal source more precisely, fine-mapping tools were created in this work. Seven Kompetitive Allele Specific PCR (KASP) primer sets were designed for SNP markers between 22 Mb and 30 Mb, six of which displayed codominant behavior and one dominant. The KASP primer sets were identified the U6718 BC1F1 subpopulation within the DNAM as segregating for bread wheat and Ae. tauschii alleles between 22 Mb and 30 Mb on 2D chromosome. Linkage maps were created for the seven D-genome chromosomes for the U6718 subpopulation. QTL analysis defined the QTL as being between 21.3 cM to 28.3 cM on the 2D linkage map and 23.3 Mb to 30.3 Mb on the physical map. Using the defined 2DS QTL and the KASP primer sets, a fine-mapping population was created consisting of five heterozygous inbred families fixed 16 unique recombination events across the 2DS QTL. These developed tools will prove instrumental tools towards refining the understanding of causal loci of this yield QTL.
Book Synopsis Plant Breeding Reviews, Volume 37 by : Jules Janick
Download or read book Plant Breeding Reviews, Volume 37 written by Jules Janick and published by John Wiley & Sons. This book was released on 2013-01-29 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Breeding Reviews presents state-of-the-art reviews on plant genetics and the breeding of all types of crops by both traditional means and molecular methods. Many of the crops widely grown today stem from a very narrow genetic base; understanding and preserving crop genetic resources is vital to the security of food systems worldwide. The emphasis of the series is on methodology, a fundamental understanding of crop genetics, and applications to major crops. It is a serial title that appears in the form of one or two volumes per year.
Book Synopsis Wheat varieties of South America: Names, parentage, pedigrees, and origins by : M. M. Kohli
Download or read book Wheat varieties of South America: Names, parentage, pedigrees, and origins written by M. M. Kohli and published by CIMMYT. This book was released on 1997 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
