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Structural Studies Of Membranes And Membrane Proteins With Atomic Force Microscopy
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Author :Daniel Mark Czajkowsky Publisher :Ann Arbor, Mich. : University Microfilms International ISBN 13 : Total Pages :470 pages Book Rating :4.:/5 (458 download)
Book Synopsis Structural Studies of Membranes and Membrane Proteins with Atomic Force Microscopy by : Daniel Mark Czajkowsky
Download or read book Structural Studies of Membranes and Membrane Proteins with Atomic Force Microscopy written by Daniel Mark Czajkowsky and published by Ann Arbor, Mich. : University Microfilms International. This book was released on 2000 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structural Analysis of Biological Membranes and Proteins by Atomic Force Microscopy by : Oliver Enders
Download or read book Structural Analysis of Biological Membranes and Proteins by Atomic Force Microscopy written by Oliver Enders and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Integrated Atomic Force Microscopy Techniques for Analysis of Biomaterials by : Laura S. Connelly
Download or read book Integrated Atomic Force Microscopy Techniques for Analysis of Biomaterials written by Laura S. Connelly and published by . This book was released on 2014 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic Force Microscopy (AFM) is the prominent techniques for structural studies of biological materials in physiological relevant fluidic environments. AFM has been used to resolve the three-dimensional (3D) surface structure of cells, membranes, and proteins structures. Ion channels, formed by membrane proteins, are the key structures that control the activity of all living systems. This dissertation focuses on the structural evaluation of membrane proteins through atomic force microscopy. In Part I, AFM is utilized to study one of the most prominent medical issues facing our society, Alzheimer's Disease (AD). AD is a misfolded protein disease characterized by the accumulation of [Beta]-amyloid (A[Beta]) peptide as senile plaques, progressive neurodegeneration, and memory loss. Recent evidence suggests that AD pathology is linked to the destabilization of cellular ionic homeostasis mediated by toxic channel structures composed of A[Beta] peptides. Selectively engineered sequences of A[Beta] were examined by AFM to elucidate the substructures and thus activity A[Beta] channels. Key residues were evaluated with the intent better understand the exact nature by which these pores conduct electrical and molecular signals, which could aid in identifying potential therapeutic targets for the prevention/treatment of AD. Additionally, AFM was used to analyze brain derived A[Beta] and newly developed pharmacological agents to study membranes and A[Beta]. Part II, presents a novel technology that incorporates electrophysiology into the AFM interface, enabling simultaneous imaging and complementary conductance measurements. The activity of ion channels is studied by various techniques, including patch clamp, free standing lipid bilayers, droplet interface bilayers, and supported lipid bilayers. However, direct correlation with channel structures has remained a challenge. The integrated atomic force microscopy system presented offers a solution to this challenge. The functionality of the system is demonstrated with an Sf9 membrane plaque containing Cx26.
Book Synopsis Biophysical Analysis of Membrane Proteins by : Eva Pebay-Peyroula
Download or read book Biophysical Analysis of Membrane Proteins written by Eva Pebay-Peyroula and published by John Wiley & Sons. This book was released on 2008-06-25 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.
Book Synopsis Imaging Membrane Proteins Using Atomic Force Microscopy Techniques by : Joan M. Lau
Download or read book Imaging Membrane Proteins Using Atomic Force Microscopy Techniques written by Joan M. Lau and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Thousands of different membrane proteins exist in the human body and reside in the membranes of cells. Structural information regarding these proteins can provide insight into the function of these proteins. Acquiring membrane protein structural information is difficult, especially when preparation techniques that are traditionally used for soluble proteins such as crystallizing and isolating proteins are not easily adapted for membrane proteins. The atomic force microscope (AFM) provides an alternative for imaging membrane proteins. The preparation required does not require crystallizing or isolating proteins, and can imagine membrane proteins in their native environment. Techniques are explored that can utilize the AFM for imaging proteins, including examining isolated cell membrane patches and membrane surfaces. One technique utilizes plasma membrane from Xenopus laevis oocytes as a model system to study membrane. A novel technique is developed to isolate oocyte membranes by bursting the oocyte and depositing its membrane on a flat mica substrate. The flat surface membrane preparation allows high-resolution AFM images to be obtained, revealing a novel structure of densely packed particles. These particles exhibit a regular, repeating pattern of a lattice-like array with orderly packing, and are thus termed 3lattice-like array particles4 (LAPs). The LAPs are orderly yet imperfectly packed, are located in depressed pools, occur with a low frequency on the oocyte membrane surface, and have not previously been seen using other isolation and imaging methods. Histogram analysis of the center-to-center distance between LAPs suggest their size to be about 44 nm in diameter, considerably larger than other reported size estimates of IMPs. These results indicate that LAPs represent a novel membrane particle organization, which merits further study. Future developments using this method or further studies to develop alternative membrane preparations may help elucidate membrane protein structure.
Book Synopsis Membrane Biophysics by : Hongda Wang
Download or read book Membrane Biophysics written by Hongda Wang and published by Springer. This book was released on 2017-11-21 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights recent advances in and diverse techniques for exploring the plasma membrane’s structure and function. It starts with two chapters reviewing the history of membrane research and listing recent advances regarding membrane structure, such as the semi-mosaic model for red blood cell membranes and the protein layer-lipid-protein island model for nucleated tissue cell membranes. It subsequently focuses on the localization and interactions of membrane components, dynamic processes of membrane transport and transmembrane signal transduction. Classic and cutting-edge techniques (e.g. high-resolution atomic force microscopy and super-resolution fluorescence microscopy) used in biophysics and chemistry are presented in a very comprehensive manner, making them useful and accessible to both researchers in the field and novices studying cell membranes. This book provides readers a deeper understanding of the plasma membrane’s organization at the single molecule level and opens a new way to reveal the relationship between the membrane’s structure and functions, making it essential reading for researchers in various fields.
Book Synopsis New Techniques for Studying Biomembranes by : Qiu-Xing Jiang
Download or read book New Techniques for Studying Biomembranes written by Qiu-Xing Jiang and published by CRC Press. This book was released on 2020-03-18 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Techniques for Studying Biomembranes describes some of the latest methods used to investigate the dynamic distribution of specific lipids in membranes and their effects on other membrane components. The contributors present important discoveries with respect to lipid analysis and lipid interactions with membrane proteins. Various methods, which have been used to study lipid bilayer structure and lipid organization in membranes, include both in vitro and in vivo membrane systems, and study membrane proteins in various membrane systems. Key Features: Reviews both in vivo and in vitro analytical technologies and methods for studying membrane structure and function Explores how lipid bilayers and membrane proteins interact Includes contributions from an international team of researchers actively studying membrane structure and function Identifies various diseases whose causes are related to membrane proteins Related Titles: Christopher R. Jacobs, Hayden Huang, and Ronald Y. Kwon. Introduction to Cell Mechanics and Mechanobiology (ISBN 978-0-8153-4425-4) Wendell Lim and Bruce Mayer. Cell Signaling: Principles and Mechanisms (ISBN 978-0-8153-4244-1) Stephen Rothman. Proteins Crossing Membranes: A Scientist’s Memoir (978-0-3670-7449-4)
Book Synopsis Membrane Proteins Production for Structural Analysis by : Isabelle Mus-Veteau
Download or read book Membrane Proteins Production for Structural Analysis written by Isabelle Mus-Veteau and published by Springer. This book was released on 2014-06-20 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomembranomics written by Hongda Wang and published by CRC Press. This book was released on 2023-11-23 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: The membrane is an intricately structured entity that performs numerous vital biological functions, including materials transport, signal transduction, energy transfer, and enzymatic reactions. Abnormal expression and distribution of certain membrane proteins are even associated with genetic disorders, neurodegenerative diseases, and malignant tumors. Despite the widely acknowledged significance of membranes, comprehensive and systematic research on membranes akin to genomics, proteomics, and metabolomics is still lacking. Furthermore, the broad concept of biomembranes is not confined to the plasma membrane alone; it also includes organelle membranes and membranes of endocytosis or exocytosis vesicles, which are also derived from the biomembrane system. This book introduces the concept of "omics" to membranes and proposes the term ‘biomembranomics.’ It compiles the latest advancements in structural analysis techniques for biomembranes, including single-molecule manipulation techniques, single-molecule fluorescence techniques, super-resolution fluorescence imaging, cryo-electron microscopy, mass spectrometry, molecular dynamics simulation, and hyphenated instrumental techniques. The book presents both classic and cutting-edge protocols in text and illustrative forms, serving as a valuable and applicable reference material. It provides a profound understanding of biomembrane organization at single-molecule level, paving new avenues for unveiling the relationship between membrane structure and function. Therefore, this book is essential reading for researchers across all related fields.
Book Synopsis Life at the Nanoscale by : Yves Dufrene
Download or read book Life at the Nanoscale written by Yves Dufrene and published by CRC Press. This book was released on 2019-03-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.
Book Synopsis Characterization of Biological Membranes by : Mu-Ping Nieh
Download or read book Characterization of Biological Membranes written by Mu-Ping Nieh and published by Walter de Gruyter GmbH & Co KG. This book was released on 2019-07-22 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of membranes has become of high importance in the fields of biology, pharmaceutical chemistry and medicine, since much of what happens in a cell or in a virus involves biological membranes. The current book is an excellent introduction to the area, which explains how modern analytical methods can be applied to study biological membranes and membrane proteins and the bioprocesses they are involved to.
Book Synopsis Structural Biology of Membrane Proteins by : Reinhard Grisshammer
Download or read book Structural Biology of Membrane Proteins written by Reinhard Grisshammer and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few years there have been many exciting and innovative developments in the field of membrane protein structure and this trend is set to continue. Structural Biology of Membrane Proteins is a new monograph covering a wide range of topics with contributions from leading experts in the field. The book is split into three sections: the first discusses topics such as expression, purification and crystallisation; the second covers characterisation techniques and the final section looks at new protein structures. The book will hence have wide appeal to researchers working in and around the field and provide an up-to-date reference source. Introductory sections to each topic are accompanied by more detailed discussions for the more experienced biochemist. Detailed descriptions of experimental methods are included to demonstrate practical approaches to membrane protein structure projects. The book also offers an up-to-date reference source in addition to descriptions of new and emerging developments, including state-of-the-art techniques for solving membrane protein structures. Structural Biology of Membrane Proteins encompasses both basic introductions and detailed descriptions of themes and should appeal to a wide range of biochemical scientists, both experienced and beginner.
Book Synopsis Biological Membranes by : Kenneth M. Merz
Download or read book Biological Membranes written by Kenneth M. Merz and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interface between a living cell and the surrounding world plays a critical role in numerous complex biological processes. Sperm/egg fusion, virus/cell fusion, exocytosis, endocytosis, and ion permeation are a few examples of processes involving membranes. In recent years, powerful tools such as X-ray crystal lography, electron microscopy, nuclear magnetic resonance, and infra-red and Raman spectroscopy have been developed to characterize the structure and dy namics of biomembranes. Despite this progress, many of the factors responsible for the function of biomembranes are still not well understood. The membrane is a very complicated supramolecular liquid-crystalline structure that is largely composed of lipids, forming a bilayer, to which proteins and other biomolecules are anchored. Often, the lipid bilayer environment is pictured as a hydropho bic structureless slab providing a thermodynamic driving force to partition the amino acids of a membrane protein according to their solubility. However, much of the molecular complexity of the phospholipid bilayer environment is ignored in such a simplified view. It is likely that the atomic details of the polar head group region and the transition from the bulk water to the hydrophobic core of the membrane are important. An understanding of the factors responsible for the function of biomembranes thus requires a better characterization at the molec ular level of how proteins interact with lipid molecules, of how lipids affect protein structure and of how lipid molecules might regulate protein function.
Book Synopsis Atomic Force Microscopy Applied to Biological Systems by : Francesco Orsini
Download or read book Atomic Force Microscopy Applied to Biological Systems written by Francesco Orsini and published by LAP Lambert Academic Publishing. This book was released on 2012-04 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the application of Atomic Force Microscopy (AFM) to the study of the Xenopus laevis oocyte plasma membrane. In particular, different sample preparation protocols developed and optimized to perform AFM investigation of both external and intracellular sides of the oocyte native plasma membrane are presented and discussed in details. AFM imaging allowed visualization and dimensional characterization of protein complexes observed on both sides of the oocyte plasma membrane. In addition, a new methodological approach based on the purification of oocyte plasma membrane by ultracentrifugation on a discontinuous sucrose gradient allowed to image oocyte membrane in a physiological-like environment as well as to identify a membrane protein, the human aquaporin 4, expressed in the oocytes thus opening interesting perspectives in the AFM investigation of heterologous proteins of relevant biomedical interest. Taken together, these results confirm the potential of AFM as a useful tool for the structural characterization of proteins in native eukaryotic membranes as well as its relevance for describing the organization of protein complexes in native biological membranes.
Book Synopsis Studies of Protein-protein Interactions at Membrane Interfaces Using Correlated Atomic Force and Fluorescence Microscopy by : James Eric Shaw
Download or read book Studies of Protein-protein Interactions at Membrane Interfaces Using Correlated Atomic Force and Fluorescence Microscopy written by James Eric Shaw and published by . This book was released on 2007 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Processes occurring at membrane interfaces are important for cellular and biological functions. The inability to readily crystallize membrane proteins for X-ray diffraction, or in solution NMR to study proteins with large molecular weight and to retain all intramolecular structures of the membrane proteins during their solubilisation process by detergents are the key reasons for a scarcity of atomic-resolution membrane protein structures. This has hindered our understanding of the structure-function relationship of membrane proteins. New strategies for studying membrane proteins will provide more insights. In this thesis, we have coupled atomic force microscopy and two fluorescence microscopy techniques---total internal reflectance fluorescence microscopy and confocal laser scanning microscopy, to visualize protein-membrane interactions and dynamics in model membrane systems. To validate our correlated approach, we investigated the partitioning of various lipid fluorescent probes in phase-separated supported planar bilayers. We applied the same methodology in two case studies of peptide-membrane interactions in supported planar bilayers: (1) indolicidin, a cationic 13 amino-acid peptide isolated from bovine neutrophils, as a model of antimicrobial peptide-membrane interactions, and (2) NAP-22 peptide, a cationic myristoylated peptide crucial for neuronal growth and plasticity, as a model of protein-induced lipid recruitment. Using this combined AFM-fluorescence approach with supported planar bilayers, we showed that: (1) the attachment of a fluorescent dye molecule can alter the physical properties of the host lipid and inhibit the lipid-dye conjugate from associating with raft domains, (2) indolicidin preferentially associates with the more disordered fluid domain and induces gel domain lowering in a concentration- and lipid-dependent manner, and (3) NAP-22 peptide induces coalescence of raft domains at low peptide concentration and phase-immersion at high peptide concentration resulting the co-localization of cholesterol and phosphatidylinositol biphosphates. This work demonstrates the versatility of a combined AFM-fluorescence approach as well as its limitations of the current configurations.
Book Synopsis Structural Studies of Proteins and Protein Complexes by Mass Spectrometry and Atomic Force Microscopy by : Stéphanie Boussert
Download or read book Structural Studies of Proteins and Protein Complexes by Mass Spectrometry and Atomic Force Microscopy written by Stéphanie Boussert and published by . This book was released on 2008 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
