Safety In Cell And Tissue Culture

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Safety in Cell and Tissue Culture

Author : G. Stacey
Publisher : Springer Science & Business Media
ISBN 13 : 940114916X
Total Pages : 257 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Safety in Cell and Tissue Culture by : G. Stacey

Download or read book Safety in Cell and Tissue Culture written by G. Stacey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cells and the detection of adven titious agents. However, it is clear that there is no room for complacency in this field and the recent expansion in the use of animal cells in the manufacture of medical products and the development of new biological assays for diagnostic and pharmaco-toxicological screening, underlines the need for vigilance regarding the correct and safe use of animal cells as substrates. This book is therefore very timely and should prove to be a highly valuable text, finding a wider audience beyond those with respon sibility for laboratory safety. The book guides the reader from fundamental cell biology issues and the establishment of new in vitro methods, through testing and validation of cell lines and on to issues in the use of animal cells in manufacturing processes.

Tissue Culture Techniques

Author : Bernice M. Martin
Publisher : Springer Science & Business Media
ISBN 13 : 1461202477
Total Pages : 251 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Tissue Culture Techniques by : Bernice M. Martin

Download or read book Tissue Culture Techniques written by Bernice M. Martin and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

General Techniques of Cell Culture

Author : Maureen A. Harrison
Publisher : Cambridge University Press
ISBN 13 : 9780521574969
Total Pages : 178 pages
Book Rating : 4.5/5 (749 download)

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Book Synopsis General Techniques of Cell Culture by : Maureen A. Harrison

Download or read book General Techniques of Cell Culture written by Maureen A. Harrison and published by Cambridge University Press. This book was released on 1997-10-13 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concise introduction to a major technique of cell biology laboratories for those new to the field.

Cell Culture Methods for In Vitro Toxicology

Author : G. Stacey
Publisher : Springer Science & Business Media
ISBN 13 : 9401709963
Total Pages : 153 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Cell Culture Methods for In Vitro Toxicology by : G. Stacey

Download or read book Cell Culture Methods for In Vitro Toxicology written by G. Stacey and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Culture Methods for in vitro Toxicology introduces the reader to a range of techniques involved in the use of in vitro cell culture in toxicological studies. It deals with major cell types studied in the field of toxicology and will be useful for anyone wishing to start work with animal cell cultures or to refresh their knowledge relating to in vitro cell models. Fundamental chapters deal with the general biology of cytotoxicity and cell immortalisation these are key issues for in vitro systems addressing the `3Rs' principle. Up-to-date overviews deal with the use of cells from liver, brain and intestine. In addition, biochemical analysis of cell responses, biotransformation pathways in cells and recombinant approaches to the early detection of cell stress are also covered in detail. Prominent features of in vitro technologies also include regulation, biosafety and standardisation. Dedicated chapters deal with these issues in a practical way in order to lead the reader to the right source of information. This book provides an up-to-date, informative and practical review of cell culture methods for in vitro toxicology. It will be of equal benefit to students and experienced toxicologists with little experience of in vitro cell culture.

Freshney's Culture of Animal Cells

Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 1119513030
Total Pages : 832 pages
Book Rating : 4.1/5 (195 download)

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Book Synopsis Freshney's Culture of Animal Cells by : R. Ian Freshney

Download or read book Freshney's Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2021-02-17 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Introduction to Cell and Tissue Culture

Author : Jennie P. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 0585275718
Total Pages : 247 pages
Book Rating : 4.5/5 (852 download)

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Book Synopsis Introduction to Cell and Tissue Culture by : Jennie P. Mather

Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Cell & Tissue Culture

Author : D. G. Newell
Publisher :
ISBN 13 :
Total Pages : 1760 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Cell & Tissue Culture by : D. G. Newell

Download or read book Cell & Tissue Culture written by D. G. Newell and published by . This book was released on 1993 with total page 1760 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and Tissue Culture provides a comprehensive collection of cell and tissue culture techniques and procedures to assist scientists with the labour intensive and expensive in vitro techniques. Specific techniques are included together with standard techniques with an Editorial Advisory Board ensuring that all procedures are written by research workers eminent in their discipline. Being a looseleaf format, the contents are easily updated, allowing for rapid dissemination of valuable revised and new techniques. In this way, information remains up-to-date, and will also expand to ensure that every topic of interest is covered. The manual should be considered an essential adjunct to any laboratory or library.

Animal Tissue Culture

Author : A Wilson Aruni
Publisher : MJP Publisher
ISBN 13 :
Total Pages : 219 pages
Book Rating : 4./5 ( download)

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Book Synopsis Animal Tissue Culture by : A Wilson Aruni

Download or read book Animal Tissue Culture written by A Wilson Aruni and published by MJP Publisher. This book was released on 2019-06-10 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and tissues culture has been one of the first and foremost techniques paving for recent cutting-edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. It has played a substantial role in the developments of health care and prophylactics industries, thus serving the mankind. It has made the dream of producing cost-effective prophylactics, diagnostics and therapeutics come true and affordable. In the recent past, with the explosion of knowledge in the field of biotechnology, intensive research in being carried out, where undergraduate and post-graduate courses are being offered in this field. Even through more emphasis is being given to theory, a dearth of practical knowledge is lacking due to paucity of established tissue culture facilities.

Basic Science Methods for Clinical Researchers

Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 382 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Tissue Culture

Author : Paul F. Jr. Kruse
Publisher : Elsevier
ISBN 13 : 0323142079
Total Pages : 896 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Tissue Culture by : Paul F. Jr. Kruse

Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Cell Culture

Author :
Publisher : Academic Press
ISBN 13 : 9780121817763
Total Pages : 668 pages
Book Rating : 4.8/5 (177 download)

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Book Synopsis Cell Culture by :

Download or read book Cell Culture written by and published by Academic Press. This book was released on 1988-06-28 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Animal Cell Culture

Author : John M. Davis
Publisher : John Wiley & Sons
ISBN 13 : 0470975636
Total Pages : 389 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Animal Cell Culture by : John M. Davis

Download or read book Animal Cell Culture written by John M. Davis and published by John Wiley & Sons. This book was released on 2011-03-16 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Culture of Animal Cells

Author : R. Ian Freshney
Publisher : Wiley-Liss
ISBN 13 : 9780471589662
Total Pages : 510 pages
Book Rating : 4.5/5 (896 download)

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Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 1993-12-29 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Basic Cell Culture Protocols

Author : Cheryl D. Helgason
Publisher : Humana Press
ISBN 13 : 9781493956524
Total Pages : 550 pages
Book Rating : 4.9/5 (565 download)

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Book Synopsis Basic Cell Culture Protocols by : Cheryl D. Helgason

Download or read book Basic Cell Culture Protocols written by Cheryl D. Helgason and published by Humana Press. This book was released on 2016-08-23 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Basic Cell Culture Protocols

Author : Jeffrey W. Pollard
Publisher : Springer Science & Business Media
ISBN 13 : 0896034410
Total Pages : 477 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard

Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Cell and Tissue Culture

Author : John Paul
Publisher :
ISBN 13 :
Total Pages : 408 pages
Book Rating : 4.:/5 (42 download)

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Book Synopsis Cell and Tissue Culture by : John Paul

Download or read book Cell and Tissue Culture written by John Paul and published by . This book was released on 1965 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant Cell and Tissue Culture for the Production of Food Ingredients

Author : Tong-Jen Fu
Publisher : Springer Science & Business Media
ISBN 13 : 1461547539
Total Pages : 286 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Plant Cell and Tissue Culture for the Production of Food Ingredients by : Tong-Jen Fu

Download or read book Plant Cell and Tissue Culture for the Production of Food Ingredients written by Tong-Jen Fu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Commercial development of cultured-derived food ingredients has attracted interna tional interest. As consumers have become more health conscious in recent years, the de mand for natural food ingredients and disease-preventative phytochemicals has increased tremendously. Plant Cell and Tissue Culture provides an alternative method for controlled production of these products. A wide range of food ingredients has been shown to be pro duced in culture. Much progress has been made in advancing this technology to the point that large-scale production has become possible. This book is developed from the Symposium "Plant Cell and Tissue Culture for Food Ingredient Production" which was held on April 13-17, 1997 at the American Chemical So ciety National Meeting in San Francisco, CA. In this book, international experts in acade mia, government, and industry discuss current advances in the field of plant cell and tissue culture with special emphasis on its application for food ingredient production. Topics re lated to various aspects of plant cell and tissue culture technology are discussed, including overviews of recent advances in plant metabolic pathway studies, process development for improving yields, and bioreactor design and operation for large-scale production. Economic considerations and issues related to the commercial development of culture-derived food in gredients are discussed. Also included are the safety assessment schemes and regulatory frameworks set up by regulatory agencies around the world.