Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
On The Mechanism Of Activation Of Rna Polymerase Ii Transcription In Saccharomyces Cerevisiae
Download On The Mechanism Of Activation Of Rna Polymerase Ii Transcription In Saccharomyces Cerevisiae full books in PDF, epub, and Kindle. Read online On The Mechanism Of Activation Of Rna Polymerase Ii Transcription In Saccharomyces Cerevisiae ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis On the Mechanism of Activation of RNA Polymerase II Transcription in Saccharomyces Cerevisiae by : Raymond Joseph Kelleher
Download or read book On the Mechanism of Activation of RNA Polymerase II Transcription in Saccharomyces Cerevisiae written by Raymond Joseph Kelleher and published by . This book was released on 1994 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mechanism and Regulation of Yeast RNA Polymerase II Transcription Initiation and Termination by : Jason Nicholas Kuehner
Download or read book Mechanism and Regulation of Yeast RNA Polymerase II Transcription Initiation and Termination written by Jason Nicholas Kuehner and published by . This book was released on 2008 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of RNA Polymerase II Transcription Initiation in the Yeast Saccharomyces Cerevisiae by : Rachel Imoberdorf
Download or read book Analysis of RNA Polymerase II Transcription Initiation in the Yeast Saccharomyces Cerevisiae written by Rachel Imoberdorf and published by . This book was released on 2004 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription occurs in a repressive chromatin context. Chromatin modifications are therefore often required to allow transcriptional activation. Here, we addressed the question whether global histone acetylation is involved in the regulation of transcriptional activation of class II promoters. We show that global histone acetylation has a role in activation of RNA polymerase II dependent transcription by modulating the inherent inhibitory effects of chromatin on the formation of the pre-initiation complex. In a second project we have developed a chromatin immunoprecipitation based assay that allows the study of transcriptional reinitiation "in vivo". Our results suggest the formation of a reinitiation scaffold on the GAL1, but not the ACT1 promoter. In a third project assessed whether TFIIB reaches promoters in the form of the holoenzyme "in vivo". Our data indicate that TFIIB does not assemble in a holoenzyme but associates with another, yet unidentified, factor(s) prior to promoter binding.
Book Synopsis Mechanism and Regulation of the Stress Response in Saccharomyces Cerevisiae by : Eric Edward Powers
Download or read book Mechanism and Regulation of the Stress Response in Saccharomyces Cerevisiae written by Eric Edward Powers and published by . This book was released on 1998 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of Elements Regulating Transcription of the Enolase Genes of Saccharomyces Cerevisiae and the Mechanism of GCR1 Control by : Catherine Elizabeth Willett
Download or read book Characterization of Elements Regulating Transcription of the Enolase Genes of Saccharomyces Cerevisiae and the Mechanism of GCR1 Control written by Catherine Elizabeth Willett and published by . This book was released on 1992 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mechanisms of Yeast RNA Polymerase II Transcription and the Role of Transcription Factor IIF by :
Download or read book Mechanisms of Yeast RNA Polymerase II Transcription and the Role of Transcription Factor IIF written by and published by . This book was released on 2006 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription of protein-coding genes by eukaryotic RNA polymerase II (RNAPII) is a multistep process that involves the concerted action of RNAPII and accessory proteins including the general transcription factors (GTFs); TFIID, TFIIB, TFIIF, TFIIE and TFIIH. The GTFs are being intensely studied to determine their function during the different stages of the transcription cycle. Numerous investigations have reported functions for the general transcription factor IIF (TFIIF) of higher eukaryotes in multiple stages of the transcription cycle, although few studies have examined the TFIIF homolog in the yeast Saccharomyces cerevisiae. The objective of this dissertation is to better understand the mechanism of action of S. cerevisiae TFIIF during the different stages of the RNAPII transcription cycle. Although the basal transcription factors are highly homologous in eukaryotes, the mechanism of transcription start site utilization on TATA-dependent promoters in S. cerevisiae is fundamentally different from that in higher eukaryotes, where the PIC assembles on the promoter and transcription initiates at a discrete site 25-30 base pairs downstream of the TATA element with the architecture of the PIC determining the initiation site. In contrast, the S. cerevisiae RNAPII machinery typically initiates at multiple sites in a window from 45 to 120 base pairs downstream of the TATA element. Results in this thesis support a transcription initiation mechanism that involves transcription-independent translocation of the yeast RNAPII to the far downstream start sites. Previous work in our laboratory identified mutations in the yeast TFIIF subunits Tfg1 and Tfg2 that confer upstream shifts in start site utilization. In vivo and in vitro studies demonstrate that TFIIF modulates the utilization of transcription start site sequences through its interaction with RNAPII. In the second and third part of this dissertation, genetic and biochemical approaches were utilized to better define TFIIF functions at post-initiation steps in the S. cerevisiae transcription cycle, and support a role for TFIIF in both promoter escape and early elongation. Combined results of this work support a model for TFIIF function where the TFIIF, through its interaction with RNAPII, affects the DNA recognition properties of the polymerase during start site utilization, promoter escape, and early elongation.
Book Synopsis Regulation of RNA Polymerase I-dependent Transcription by the Ribosomal DNA Enhancer in Saccharomyces Cerevisiae by : John Jun Kang
Download or read book Regulation of RNA Polymerase I-dependent Transcription by the Ribosomal DNA Enhancer in Saccharomyces Cerevisiae written by John Jun Kang and published by . This book was released on 1992 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biochemical and Genetic Characterization of the Transcription Elongation Factor TFIIS from the Yeast Saccharomyces Cerevisiae by : Karen Renee Christie
Download or read book Biochemical and Genetic Characterization of the Transcription Elongation Factor TFIIS from the Yeast Saccharomyces Cerevisiae written by Karen Renee Christie and published by . This book was released on 1995 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of the process of transcriptional elongation is an important control mechanism in the expression of some genes. To fully understand this form of regulation will require better understanding of the functions of transcription elongation factors. The goal of this work was to characterize the transcription elongation factor TFIIS from Saccharomyces cerevisiae, originally called P37. I demonstrated that, like the mammalian TFIIS proteins, the yeast protein stimulates RNA polymerase II to cleave the nascent RNA transcript and to read-through an intrinsic block to elongation. Investigation of the protein-protein contacts between TFIIS and RNA polymerase II indicated that the carboxyl-terminal domain of the largest subunit, subunit four, and subunit seven of the polymerase are not required for TFIIS to promote cleavage and read-through by the polymerase. In addition the carboxyl-terminal half of the yeast TFIIS protein is sufficient for both of these in vitro activities. This result is consistent with the previous results demonstrating the carboxyl-terminus of mouse TFIIS was sufficient to activate RNA polymerase in vitro.
Book Synopsis RNA Binding to RNA Polymerase II Purified from the Yeast Saccharomyces Cerevisiae by : Tracy Lanise Johnson
Download or read book RNA Binding to RNA Polymerase II Purified from the Yeast Saccharomyces Cerevisiae written by Tracy Lanise Johnson and published by . This book was released on 1996 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Activation of RNA Polymerase II Mediated Transcription by : Andrew Emili
Download or read book Activation of RNA Polymerase II Mediated Transcription written by Andrew Emili and published by . This book was released on 1997 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: I have developed a sensitive and highly selective in vitro crosslinking strategy to characterize the protein-protein interactions mediated by a sequence-specific activator of transcription with components of the RNA polymerase II transcriptional machinery. The basis of this approach involved the selective modification of the chimeric transactivator LexA-E2F-1 with the photoreactive crosslinking reagent maleimide-4-benzophenone at a single cysteine residue located within its activation domain. Using this approach, I have demonstrated that LexA-E2F-1 can interact in a direct and binding-site-dependent manner with the TATA-binding protein TBP. I provided evidence that this interaction is biologically relevant by showing that mutations within the E2F-1 activation domain which impair activation by LexA-E2F-1 also reduce crosslinking of LexA-E2F-1 to TBP. I have refined my original crosslinking methodology in order to identify additional protein targets of LexA-E2F-1 in an in vitro transcription system derived from a yeast cell extract. Using this approach, I have shown that the activation domain of LexA-E2F-1 interacts in a promoter-dependent manner with a novel component of the yeast RNA polymerase II transcriptional machinery, XTC1. The XTC1 gene product also interacts directly with the activation domains of the herpes virion protein VP16 and the yeast activator GAL4, suggesting it is a common target of activators. Yeast strains deleted for the XTC1 gene exhibit growth defects and altered responses of the RNA polymerase II transcriptional machinery to activators in vivo consistent with XTC1 being a physiologically relevant target of activators in yeast. Finally, I have performed affinity chromatography experiments aimed at identifying human proteins which interact with the evolutionarily conserved carboxy-terminal domain (CTD) of the largest polypeptide subunit of the RNA polymerase II. I have purified and identified two such CTD-binding proteins as the essential splicing factor PSF and the putative splicing factor p54$\rm\sp{nrb}.$ Since splicing of messenger RNA is intimately coupled to the process of transcriptional elongation in vivo, this observation suggests that the CTD may be directly involved in the processing of nascent RNA transcripts in addition to its role in regulating transcription by Pol II.
Book Synopsis Combinatorial Regulation of HO Transcription in Saccharomyces Cerevisiae by : Ramon Kenneth Tabtiang
Download or read book Combinatorial Regulation of HO Transcription in Saccharomyces Cerevisiae written by Ramon Kenneth Tabtiang and published by . This book was released on 1998 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation and Variation of Subunits of RNA Polymerase II in Saccharomyces Cerevisiae by : David Jansma
Download or read book Regulation and Variation of Subunits of RNA Polymerase II in Saccharomyces Cerevisiae written by David Jansma and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase (RNAP) uses ribonucleoside triphosphates as a substrate to form RNA chains. The RNA is a faithful copy of one strand of a double-stranded DNA template along which RNAP moves while making the RNA. This process (called transcription) is highly regulated such that DNA-encoded genes are transcribed at a large variety of rates and at different times in response to cues within and outside the cell. The goal of my thesis work was to investigate the mechanisms that regulate the synthesis of RNAP in yeast cells. I focused on the form of RNAP (RNAPII) of the yeast, 'Saccharomyces cerevisiae', that is responsible primarily for transcribing genes that encode proteins. I have shown that a 10-fold reduction in the level of the largest subunit of RNAPII, and likely the level of RNAPII itself, causes slow growth, temperature-sensitivity, and the inability to grow on medium lacking inositol. Hence, the level of RNAPII must be carefully maintained for normal cell growth. I next examined elements that control the synthesis of RNAPII. I have demonstrated that the DNA sequences which are upstream of the genes encoding the two largest subunits of RNAPII, namely 'RPO21' and 'RPO22', contain binding sites for two abundant transcription factors called Abf1p and Reb1p, and thymidine-rich sequences downstream of these binding sites. Both the binding sites and the T-rich regions are important for the expression of these genes. An examination of the upstream sequences of other RNAPII subunit genes revealed binding sites for Abf1p and Reb1p as well as nearby thymidine-rich sequences. This may indicate that there is a mechanism for the coordinate synthesis of RNAPII subunit genes. I sought evidence for a feedback regulatory mechanism that may control the synthesis of RNAPII. Either the underproduction of Rpo21p, or the depletion of Fcp1p, an RNAPII phosphatase that has a critical role in transcription, leads to a 5-fold increase in the expression of a reporter gene that is controlled by 'RPO21' regulatory sequences. The increase is not observed with other subunits. I discuss the implications of these results and future directions.
Book Synopsis Methods in Yeast Genetics by : David C. Amberg
Download or read book Methods in Yeast Genetics written by David C. Amberg and published by CSHL Press. This book was released on 2005 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Book Synopsis Molecular Mechanism for Degradation of Transcriptionally Stalled RNA Polymerase II in the Yeast Saccharomyces Cerevisiae by : Eleni Karakasili
Download or read book Molecular Mechanism for Degradation of Transcriptionally Stalled RNA Polymerase II in the Yeast Saccharomyces Cerevisiae written by Eleni Karakasili and published by . This book was released on 2010 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book 科学衛星計画シンポジウム written by and published by . This book was released on 1965 with total page 87 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of RNA Polymerase II Transcription by the SPT Proteins in Yeast Saccharomyces Cerevisiae by : Lei Zhang
Download or read book Regulation of RNA Polymerase II Transcription by the SPT Proteins in Yeast Saccharomyces Cerevisiae written by Lei Zhang and published by . This book was released on 2005 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of RNA Polymerase II Transcription by Spt4 in Saccharomyces Cerevisiae by : Ulku Uzun
Download or read book Regulation of RNA Polymerase II Transcription by Spt4 in Saccharomyces Cerevisiae written by Ulku Uzun and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
