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Microscopy Optical Spectroscopy And Macroscopic Techniques
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Book Synopsis Microscopy, Optical Spectroscopy, and Macroscopic Techniques by : Christopher Jones
Download or read book Microscopy, Optical Spectroscopy, and Macroscopic Techniques written by Christopher Jones and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.
Book Synopsis Optical Spectroscopic and Microscopic Techniques by : Harekrushna Sahoo
Download or read book Optical Spectroscopic and Microscopic Techniques written by Harekrushna Sahoo and published by Springer Nature. This book was released on 2022-02-25 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
Book Synopsis Microscopy Techniques by : Jens Rietdorf
Download or read book Microscopy Techniques written by Jens Rietdorf and published by Springer Science & Business Media. This book was released on 2005-06-23 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts
Book Synopsis Calorimetry by : Stefan Mathias Sarge
Download or read book Calorimetry written by Stefan Mathias Sarge and published by John Wiley & Sons. This book was released on 2014-02-25 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clearly divided into three parts, this practical book begins by dealing with all fundamental aspects of calorimetry. The second part looks at the equipment used and new developments. The third and final section provides measurement guidelines in order to obtain the best results. The result is optimized knowledge for users of this technique, supplemented with practical tips and tricks.
Book Synopsis Microscopic and Spectroscopic Imaging of the Chemical State by : Michael D. Morris
Download or read book Microscopic and Spectroscopic Imaging of the Chemical State written by Michael D. Morris and published by CRC Press. This book was released on 1993-07-26 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents chemical state imaging methods useful on distance scales ranging from individual atoms to millimeters. This work is intended for chemists familiar with modern spectroscopies, but includes tutorial material on basic imaging processes for those with little background in the field.
Book Synopsis Oxygen Transport to Tissue XXVII by : Giuseppe Cicco
Download or read book Oxygen Transport to Tissue XXVII written by Giuseppe Cicco and published by Springer Science & Business Media. This book was released on 2006-11-22 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the proceedings of the 32nd scientific meeting of the International Society on Oxygen Transport to Tissue (ISOTT) in Bari, Italy, August 21-26, 2004. It covers all aspects of oxygen delivery to tissue, including blood flow and its regulation as well as oxygen metabolism. Special emphasis is placed on methods of oxygen measurement in living tissue and application of these technologies to understanding physiological and biochemical basis for pathology related to tissue oxygenation. The event hosted was a multidisciplinary meeting designed to bring together experts and students from a range of research fields.
Book Synopsis Structure and Mechanism in Protein Science by : Alan Fersht
Download or read book Structure and Mechanism in Protein Science written by Alan Fersht and published by Macmillan. This book was released on 1999 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes four new chapters which focus on the repercussions of protein engineering for protein folding and catalysis, this new edition is a more general guide to mechanism in protein science.
Book Synopsis DNA-protein Interactions by : Andrew Arthur Travers
Download or read book DNA-protein Interactions written by Andrew Arthur Travers and published by Oxford University Press, USA. This book was released on 2000 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.
Book Synopsis Biocalorimetry 2 by : John E. Ladbury
Download or read book Biocalorimetry 2 written by John E. Ladbury and published by John Wiley & Sons. This book was released on 2005-07-15 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, high-sensitivity calorimetry has developed from a specialist method used mainly by dedicated experts to a major, commercially available tool in the arsenal directed at understanding molecular interactions and stability. Calorimeters have now become commonplace in bioscience laboratories. As a result, the number of those proficient in experimentation in this field has risen dramatically, as has the range of experiments to which these methods have been applied. Applications extend from studies in small molecule and solvent biophysics, through drug screening to whole cell assays. The technology has developed to include higher levels of sensitivity (and hence smaller sample size requirements) and a drive towards high-throughput technology, creating a very large user base in both academia and the pharmaceutical industry. This book is a fully revised and updated edition of the successful Biocalorimetry: Applications of Calorimetry in the Biological Sciences, published in 1998. Since then, there have been many advances in the instrumentation as well as in its applications and methodology. There are general chapters highlighting the usage of the isothermal titration calorimeter and the differential scanning calorimeter, more advanced chapters on specific applications and tutorials that cover the idiosyncrasies of experimental methods and data analysis. The book draws these together to create the definitive biological calorimetric text book. This book both explains the background to the method and describes novel, high-impact applications. It features works of interest to the experienced calorimetrist and the enthusiastic dilettante. The book should be of interest to all working in the field of biocalorimetry, from graduate students to researchers in academia and in industry.
Book Synopsis Immunocytochemical Methods and Protocols by : Lorette C. Javois
Download or read book Immunocytochemical Methods and Protocols written by Lorette C. Javois and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth ods has spread to a wide array of biological disciplines. Early applica tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic development. For a select few disci plines, volumes have been published focusing on the specific applica tion of immunocytochemical techniques to that discipline. What distinguishes the present book, Immunocytochemical Meth ods and Protocols, from earlier books is its broad appeal to researchers in all disciplines, including those in both research and clinical settings. The methods and protocols presented here are designed to be general in their application and the accompanying "Notes" provide invaluable assistance in adapting or troubleshooting the protocols. Interspersed throughout the book are chapters providing overviews of select topics related to immunocytochemistry.
Book Synopsis Computer Analysis of Sequence Data, Part I by : Annette M. Griffin
Download or read book Computer Analysis of Sequence Data, Part I written by Annette M. Griffin and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gen- ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as the human genome project, where vast tracts of DNA of unknown function are sequenced. This sheer volume of available data makes advanced computer methods ess- tial to analysis, and a familiarity with computers and sequence ana- sis software a vital requirement for the researcher involved with DNA sequencing. Even for nonsequencers, a familiarity with sequence analysis software can be important. For instance, gene sequences already present in the databases can be extremely useful in the design of cloning and genetic manipulation experiments. This two-part work on Analysis of Data is designed to be a practical aid to the researcher who uses computers for the acquisition, storage, or analysis of nucleic acid (and/or p- tein) sequences. Each chapter is written such that a competent sci- tist with basic computer literacy can carry out the procedure successfully at the first attempt by simply following the detailed pr- tical instructions that have been described by the author. A Notes section, which is included at the end of each chapter, provides advice on overcoming the common problems and pitfalls sometimes enco- tered by users of the sequence analysis software. Software packages for both the mainframe and personal computers are described.
Book Synopsis Protein Targeting Protocols by : Roger A. Clegg
Download or read book Protein Targeting Protocols written by Roger A. Clegg and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.
Book Synopsis Chromosome Analysis Protocols by : John R. Gosden
Download or read book Chromosome Analysis Protocols written by John R. Gosden and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports—as a result of pressure on space from editors, and the response to that pressure by authors—contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.
Book Synopsis Advanced Environmental Analysis by : Chaudhery Mustansar Hussain
Download or read book Advanced Environmental Analysis written by Chaudhery Mustansar Hussain and published by Royal Society of Chemistry. This book was released on 2016-11-14 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Environmental analysis techniques have advanced due to the use of nanotechnologies in improving the detection sensitivity and miniaturization of the devices in analytical procedures. These allow for developments such as increases in analyte concentration, the removal of interfering species and improvements in the detection limits. Bridging a gap in the literature, this book uniquely brings together state-of-the-art research in the applications of novel nanomaterials to each of the classical components of environmental analysis, namely sample preparation and extraction, separation and identification by spectroscopic techniques. Special attention is paid to those approaches that are considered greener and reduce the cost of the analysis process both in terms of chemicals and time consumption. Advanced undergraduates, graduates and researchers at the forefront of environmental science and engineering will find this book a good source of information. It will also help regulators, decision makers, surveillance agencies and the organizations assessing the impact of pollutants on the environment.
Book Synopsis Signal Transduction Protocols by : David A. Kendall
Download or read book Signal Transduction Protocols written by David A. Kendall and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: As our understanding of the biological sciences expands, the bou- aries between traditional disciplines tend to blur at the edges. Physio- gists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysi- ogy and have instead concentrated on more biochemical approaches.
Book Synopsis DNA'Protein Interactions by : G. Geoff Kneale
Download or read book DNA'Protein Interactions written by G. Geoff Kneale and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of protein-nucleic acid interactions is currently one of the most rapidly growing areas of molecular biology. DNA binding proteins are at the very heart of the regulation and control of gene expression, replication, and recombination: Enzymes that recognize and either modify or cleave specific DNA sequences are equally important to the cell. Some of the techniques reported in this volume can be used to identify previously unknown DNA binding proteins from crude cell extracts. Virtually all are capable of giving direct information on the molecular basis of the interaction—the location of the DNA binding site; the strength and specificity of binding; the identities of individual groups on specific bases involved in binding; the specific amino acid residues of the protein that interact with the DNA; or the effects of protein binding on gross conformation and local structure of DNA. The recognition of DNA sequences by proteins is a complex phenomenon, involving specific hydrogen bonding contacts to the DNA bases ("direct readout") and/or interactions with the sugar-phos phate backbone ("indirect readout"). The latter interactions can also be highly specific because of sequence-dependent conformational changes in the DNA. In addition, intercalation of planar aromatic amino acid side-chains between the DNA bases can occur, most notably with single-stranded DNA binding proteins. Furthermore, when bound, many DNA binding proteins induce drastic structural changes in the DNA as an integral part of their function.
Book Synopsis Cryopreservation and Freeze-Drying Protocols by : John G. Day
Download or read book Cryopreservation and Freeze-Drying Protocols written by John G. Day and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: The storage of biological material for regular or future use is a fundamental requirement in many biological and medical sciences. Cryopreservation and freeze-drying are the preferred techniques for achieving long-term storage, and have been applied to a diverse range of biological materials. Though the basis for many methodologies is common, laboratories frequently lack expertise with the correct storage procedures, so that many apply outdated or inappropriate protocols for storing their samples or cultures. Cryopreservation and Freeze-Drying Protocols is a compilation of the many and varied methodologies that have been developed in expert laboratories. The protocols are reproducible, robust, and in most instances have been transferred quite successfully to other laboratories. Our intended readers are those proposing to establish or improve biostorage systems in their own laboratories or units, whether concerned with culture collections, animal husbandry, aquaculture, or human fertilization programs. Because the emphasis of Cryopreservation and Freeze-Drying Protocols is on methodology, it is our intention to provide readers with the tools to make practical progress without reference to other sources. Each chapter deals with an organelle, cell, or tissue type: a short int- duction on the status of its biostorage development is followed by a detailed description of the materials required and a methodological p- tocol to be followed, with explanatory notes. This is very much a first edition; we hope and trust that future editions will contain cryopreservation and freeze-drying protocols for ceils, tissues, and organs that are at present still recalcitrant to succe- ful preservation.
