Microscopy, Optical Spectroscopy, and Macroscopic Techniques

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Publisher : Springer Science & Business Media
ISBN 13 : 159259509X
Total Pages : 260 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Microscopy, Optical Spectroscopy, and Macroscopic Techniques by : Christopher Jones

Download or read book Microscopy, Optical Spectroscopy, and Macroscopic Techniques written by Christopher Jones and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.

Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications

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Author :
Publisher : Frontiers Media SA
ISBN 13 : 288971523X
Total Pages : 221 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications by : Vicente Micó

Download or read book Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and published by Frontiers Media SA. This book was released on 2021-10-22 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:

New Techniques of Optical Microscopy and Microspectroscopy

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Publisher :
ISBN 13 : 9780333491089
Total Pages : 279 pages
Book Rating : 4.4/5 (91 download)

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Book Synopsis New Techniques of Optical Microscopy and Microspectroscopy by : Richard J. Cherry

Download or read book New Techniques of Optical Microscopy and Microspectroscopy written by Richard J. Cherry and published by . This book was released on 1991 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of various techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutionizing the field of optical microscopy. This book intends to bring together the various advances in order to provide an account of what can be achieved with modern optical microscopic methods.

Optical Spectroscopic and Microscopic Techniques

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Publisher : Springer Nature
ISBN 13 : 9811645507
Total Pages : 260 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis Optical Spectroscopic and Microscopic Techniques by : Harekrushna Sahoo

Download or read book Optical Spectroscopic and Microscopic Techniques written by Harekrushna Sahoo and published by Springer Nature. This book was released on 2022-02-25 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.

A Practical Guide to Optical Microscopy

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Publisher : CRC Press
ISBN 13 : 1351630350
Total Pages : 247 pages
Book Rating : 4.3/5 (516 download)

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Book Synopsis A Practical Guide to Optical Microscopy by : John Girkin

Download or read book A Practical Guide to Optical Microscopy written by John Girkin and published by CRC Press. This book was released on 2019-06-14 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Microscopic and Spectroscopic Imaging of the Chemical State

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Publisher : CRC Press
ISBN 13 : 1482277530
Total Pages : 504 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Microscopic and Spectroscopic Imaging of the Chemical State by : Michael D. Morris

Download or read book Microscopic and Spectroscopic Imaging of the Chemical State written by Michael D. Morris and published by CRC Press. This book was released on 1993-07-26 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents chemical state imaging methods useful on distance scales ranging from individual atoms to millimeters. This work is intended for chemists familiar with modern spectroscopies, but includes tutorial material on basic imaging processes for those with little background in the field.

Methods in Plant Electron Microscopy and Cytochemistry

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Publisher : Springer Science & Business Media
ISBN 13 : 1592592325
Total Pages : 297 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Methods in Plant Electron Microscopy and Cytochemistry by : William V. Dashek

Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

Microscopic Methods in Metals

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Publisher : Springer Science & Business Media
ISBN 13 : 3642465714
Total Pages : 470 pages
Book Rating : 4.6/5 (424 download)

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Book Synopsis Microscopic Methods in Metals by : Ulrich Gonser

Download or read book Microscopic Methods in Metals written by Ulrich Gonser and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods of scientific investigation can be divided into two categories: they are either macroscopic or microscopic in nature. The former are generally older, classical methods where the sample as a whole is studied and various local prop erties are deduced by differentiation. The microscopic methods, on the other hand, have been discovered and developed more recently, and they operate for the most part on an atomistic scale. Glancing through the shelves of books on the various scientific fields, and, in particular, on the field of physical metallurgy, we are surprised at how lit tle consideration has been given to the microscopic methods. How these tools provide new insight and information is a question which so far has not at tracted much attention. Similar observations can be made at scientific confer ences, where the presentation of papers involving microscopic methods is often pushed into a far corner. This has led users of such methods to organize their own special conferences. The aim of this book is to bridge the present gap and encourage more interaction between the various fields of study and selected microscopic meth ods, with special emphasis on their suitability for investigating metals. In each case the principles of the method are reviewed, the advantages and successes pointed out, but also the shortcomings and limitations indicated.

Optical Techniques in Biological Research

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Publisher :
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Optical Techniques in Biological Research by : Denis L. Rousseau

Download or read book Optical Techniques in Biological Research written by Denis L. Rousseau and published by . This book was released on 1984 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Light scattering / Bennie R. Ware -- Raman scattering / Denis L. Rousseau and Mark R. Ondrias -- Fourier transform infrared spectroscopy / James O. Alben and Frank G. Fiamingo -- Nanosecond fluorescence spectroscopy / Juan Yguerabide and Evangelina E. Yguerabide -- Circular dichroism / Marcos F. Maestre -- Electron microscopy / S.W. Hui.

Protein Targeting Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592595723
Total Pages : 331 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Protein Targeting Protocols by : Roger A. Clegg

Download or read book Protein Targeting Protocols written by Roger A. Clegg and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.

Superresolution Optical Microscopy

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Publisher : Springer
ISBN 13 : 9783030216931
Total Pages : 0 pages
Book Rating : 4.2/5 (169 download)

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Book Synopsis Superresolution Optical Microscopy by : Barry R. Masters

Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer. This book was released on 2021-08-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Microscopic Techniques for the Non-Expert

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Publisher : Springer Nature
ISBN 13 : 3030995429
Total Pages : 251 pages
Book Rating : 4.0/5 (39 download)

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Book Synopsis Microscopic Techniques for the Non-Expert by : Sathish-Kumar Kamaraj

Download or read book Microscopic Techniques for the Non-Expert written by Sathish-Kumar Kamaraj and published by Springer Nature. This book was released on 2022-06-27 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers fundamental microscopic techniques for Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), and other microscopic tools. It provides step-by-step instructions and explanations of the basic fundamental concepts and mechanisms and guides the reader on resolving queries related to taking and analyzing microscopy images. The latest advancements and developments in microscopic equipment are described. Theoretical background on microscopy is also provided to enhance the reader’s understanding of microscopy techniques and tools. Microscopic Techniques for the Non-Expert is an ideal book for undergraduate and postgraduate students, as well as researchers with a background in environmental science, materials science, biomedicine, engineering, or bio-nanotechnology.

Plant Cell Biology

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Publisher : CRC Press
ISBN 13 : 1482294494
Total Pages : 507 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Plant Cell Biology by : William V Dashek

Download or read book Plant Cell Biology written by William V Dashek and published by CRC Press. This book was released on 2010-03-09 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: While there are a few plant cell biology books that are currently available, these are expensive, methods-oriented monographs. The present volume is a textbook for upper undergraduate and beginning graduate students. This textbook stresses concepts and is inquiry-oriented. To this end, there is extensive use of original research literature. As we live in an era of literature explosion, one must be selective. These judgements will naturally vary with each investigator. Input was sought from colleagues in deciding the literature to include. In addition to provision of select research literature, this volume presents citations and summaries of certain laboratory methods. In this connection, the textbook stresses quantitative data to enhance the student?s analytical abilities. Thus the volume contains computer-spread sheets and references to statistical packages, e.g. Harvard Graphics and Statistica.

Methods in Protein Structure and Stability Analysis: Vibrational spectroscopy

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Publisher : Nova Publishers
ISBN 13 : 9781600217036
Total Pages : 326 pages
Book Rating : 4.2/5 (17 download)

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Book Synopsis Methods in Protein Structure and Stability Analysis: Vibrational spectroscopy by : Vladimir N. Uversky

Download or read book Methods in Protein Structure and Stability Analysis: Vibrational spectroscopy written by Vladimir N. Uversky and published by Nova Publishers. This book was released on 2007 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.

Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics

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Publisher : Elsevier
ISBN 13 : 0323993664
Total Pages : 560 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics by : Prakash Saudagar

Download or read book Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics written by Prakash Saudagar and published by Elsevier. This book was released on 2022-09-28 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics presents the latest emerging technologies in spectroscopy and advances in established spectroscopic methods. The book presents a guide to research methods in biomolecular spectroscopy, providing comprehensive coverage of developments in the spectroscopic techniques used to study protein structure and dynamics. Seventeen chapters from leading researchers cover key aspects of spectroscopic methods, with each chapter covering structure, folding, and dynamics. This title will help researchers keep up-to-date on the latest novel methods and advances in established methods. Presents current, emerging, and evolving advances and applications of spectroscopic techniques in the study of biomolecules, including proteins and nucleic acids Discusses contemporary spectroscopic techniques used to study biomolecular structure, interaction, and dynamics

Progress in Medicinal Chemistry

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Publisher : Elsevier
ISBN 13 : 9780080862866
Total Pages : 393 pages
Book Rating : 4.8/5 (628 download)

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Book Synopsis Progress in Medicinal Chemistry by :

Download or read book Progress in Medicinal Chemistry written by and published by Elsevier. This book was released on 2001-03-01 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in Medicinal Chemistry

Selected Papers on Optical Microscopy

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Publisher : SPIE-International Society for Optical Engineering
ISBN 13 :
Total Pages : 632 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Selected Papers on Optical Microscopy by : Marion B. Rhodes

Download or read book Selected Papers on Optical Microscopy written by Marion B. Rhodes and published by SPIE-International Society for Optical Engineering. This book was released on 2000 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: SPIE Milestones are collections of seminal papers from the world literature covering important discoveries and developments in optics and photonics.