Manipulating the Mouse Embryo

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Author :
Publisher : Cold Spring Harbor Laboratory Press
ISBN 13 :
Total Pages : 352 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Manipulating the Mouse Embryo by : Brigid Hogan

Download or read book Manipulating the Mouse Embryo written by Brigid Hogan and published by Cold Spring Harbor Laboratory Press. This book was released on 1986 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.

Manipulating the Mouse Embryo

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Author :
Publisher :
ISBN 13 : 9781936113019
Total Pages : 0 pages
Book Rating : 4.1/5 (13 download)

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Book Synopsis Manipulating the Mouse Embryo by : Richard Behringer

Download or read book Manipulating the Mouse Embryo written by Richard Behringer and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.

Manipulating the Mouse Embryo

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Author :
Publisher : Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press
ISBN 13 :
Total Pages : 784 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Manipulating the Mouse Embryo by : Andras Nagy

Download or read book Manipulating the Mouse Embryo written by Andras Nagy and published by Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press. This book was released on 2003 with total page 784 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.

Manipulating the Mouse Embryo

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Publisher :
ISBN 13 : 9780879693923
Total Pages : 500 pages
Book Rating : 4.6/5 (939 download)

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Book Synopsis Manipulating the Mouse Embryo by : Brigid Hogan

Download or read book Manipulating the Mouse Embryo written by Brigid Hogan and published by . This book was released on 1994-01-01 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:

小鼠胚胎操作实验指南

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Publisher :
ISBN 13 : 9787030124388
Total Pages : 764 pages
Book Rating : 4.1/5 (243 download)

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Book Synopsis 小鼠胚胎操作实验指南 by : A.·纳吉 (加)

Download or read book 小鼠胚胎操作实验指南 written by A.·纳吉 (加) and published by . This book was released on 2003 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: 本书其余责任者还有:(加)M. 格斯滕斯坦、(德)K. 文特斯滕、(美)R. 贝林杰。

Mouse Development

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Publisher : Gulf Professional Publishing
ISBN 13 : 9780125979511
Total Pages : 754 pages
Book Rating : 4.9/5 (795 download)

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Book Synopsis Mouse Development by : Janet Rossant

Download or read book Mouse Development written by Janet Rossant and published by Gulf Professional Publishing. This book was released on 2002-03 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book represents a classic compilation of current knowledge about mouse development and its correlates to research in cell biology, molecular biology, genetics, and neuroscience. Emphasis is placed on the research strategy, experimental design, and critical analysis of the data, disguishing this from other books that only focus on protocols for mouse developmental research. Selected chapters are indexed to electronic databases such as GeneBank, GenBank, Electronic Mouse Atlas, and Transgenic/Knockout, further increasing the utility of this book as a reference. *Broad-based overview of mouse development from fundamental to specialist levels *Extensive coverage of a wide range of developmental mutations of the mouse *Excellent benchmark illustrations of brain, craniofacial, gut and heart development *In-depth experiment-based assessment of concepts in mammalian development *Focus on models of specific relevance to human development *Comprehensive reference to key literature and electronic databases related to mouse development *High-quality full-color production

Stem Cells and Tissue Repair

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Publisher : Humana
ISBN 13 : 9781071606575
Total Pages : 214 pages
Book Rating : 4.6/5 (65 download)

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Book Synopsis Stem Cells and Tissue Repair by : Chrissa Kioussi

Download or read book Stem Cells and Tissue Repair written by Chrissa Kioussi and published by Humana. This book was released on 2021-06-14 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Anatomical Basis of Mouse Development

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Publisher : Academic Press
ISBN 13 : 0080573371
Total Pages : 306 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis The Anatomical Basis of Mouse Development by : Matthew H. Kaufman

Download or read book The Anatomical Basis of Mouse Development written by Matthew H. Kaufman and published by Academic Press. This book was released on 1999-03-10 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an essential anatomical resource for developmental biologists who need to know about any aspect of mouse developmental anatomy, as well as for geneticists using the mouse embryo as a model. The book is a companion to Kaufman's The Atlas of Mouse Development, and details the developmental anatomy of the early embryo, the transitional tissues, and all the major organ systems. It also provides extensive comparisons with human developmental anatomy, both normal and abnormal. The book has extensive reference indexes detailing developmental stage criteria. The Anatomical Basis of Mouse Development will be a key reference work for anyone who needs to understand developmental anatomy in normal and mutant mice. Complements Kaufman's The Atlas of Mouse Development Gives anatomical descriptions from oogenesis to birth, at a level of detail that goes beyond that found in most literature Provides detailed explanations for geneticists and molecular biologists with limited anatomical background to help them understand the emergence of all the major structures in the mouse embryo Contains comprehensive indexes detailing the appearance of over 1000 organs, tissues, and their components at different stages of mouse embryogenesis Includes comparisons with normal and abnormal human development Contains over 100 clear line diagrams showing mouse developmental anatomy as well as lineage relationships for the major organ systems

A Laboratory Guide to the Mammalian Embryo

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Author :
Publisher : Oxford University Press
ISBN 13 : 9780195142266
Total Pages : 434 pages
Book Rating : 4.1/5 (422 download)

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Book Synopsis A Laboratory Guide to the Mammalian Embryo by : David K. Gardner

Download or read book A Laboratory Guide to the Mammalian Embryo written by David K. Gardner and published by Oxford University Press. This book was released on 2004-01-08 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research

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Author :
Publisher : National Academies Press
ISBN 13 : 030916785X
Total Pages : 223 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research by : National Research Council

Download or read book Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research written by National Research Council and published by National Academies Press. This book was released on 2003-08-22 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of these animals, and guidance on adapting these guidelines to various situations without hindering the research process. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research offers a more in-depth treatment of concerns specific to these disciplines than any previous guide on animal care and use. It treats on such important subjects as: The important role that the researcher and veterinarian play in developing animal protocols. Methods for assessing and ensuring an animal's well-being. General animal-care elements as they apply to neuroscience and behavioral research, and common animal welfare challenges this research can pose. The use of professional judgment and careful interpretation of regulations and guidelines to develop performance standards ensuring animal well-being and high-quality research. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research treats the development and evaluation of animal-use protocols as a decision-making process, not just a decision. To this end, it presents the most current, in-depth information about the best practices for animal care and use, as they pertain to the intricacies of neuroscience and behavioral research.

Mouse Phenotypes

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Publisher : CSHL Press
ISBN 13 : 9780879696405
Total Pages : 260 pages
Book Rating : 4.6/5 (964 download)

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Book Synopsis Mouse Phenotypes by : Virginia E. Papaioannou

Download or read book Mouse Phenotypes written by Virginia E. Papaioannou and published by CSHL Press. This book was released on 2005 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides step–by–step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects.

New Insights into Theriogenology

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Author :
Publisher : BoD – Books on Demand
ISBN 13 : 1789847729
Total Pages : 162 pages
Book Rating : 4.7/5 (898 download)

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Book Synopsis New Insights into Theriogenology by :

Download or read book New Insights into Theriogenology written by and published by BoD – Books on Demand. This book was released on 2018-12-05 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers a variety of topics on animal reproduction and reproductive medicine. With evolving technology and a continual increase in knowledge, regarding domestic pets or agricultural animals, new information is available on diverse topics in this broad field. The book contents reflect the individual experience of authors, who developed a number of themes identified as attracting interest in the field. As it is, new opportunities were opened for productive collaboration. We have tried to provide you with current, specialised information that may be useful to students, clinicians and researchers. We hope this book inspires you to embrace these themes, foster the debate on particular topics and may be used as a start-up source for exploring the theriogenology field.

Mouse Molecular Embryology

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Publisher : Humana
ISBN 13 : 9781489977731
Total Pages : 0 pages
Book Rating : 4.9/5 (777 download)

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Book Synopsis Mouse Molecular Embryology by : Mark Lewandoski

Download or read book Mouse Molecular Embryology written by Mark Lewandoski and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

Scientific Frontiers in Developmental Toxicology and Risk Assessment

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Author :
Publisher : National Academies Press
ISBN 13 : 0309070864
Total Pages : 348 pages
Book Rating : 4.3/5 (9 download)

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Book Synopsis Scientific Frontiers in Developmental Toxicology and Risk Assessment by : National Research Council

Download or read book Scientific Frontiers in Developmental Toxicology and Risk Assessment written by National Research Council and published by National Academies Press. This book was released on 2000-12-21 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.

The Anatomical Basis of Mouse Development

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Author :
Publisher : Gulf Professional Publishing
ISBN 13 : 0124020607
Total Pages : 306 pages
Book Rating : 4.1/5 (24 download)

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Book Synopsis The Anatomical Basis of Mouse Development by : Matthew H. Kaufman

Download or read book The Anatomical Basis of Mouse Development written by Matthew H. Kaufman and published by Gulf Professional Publishing. This book was released on 1999-03-03 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Anatomical Basis of Mouse Development by Kaufman and Bard is an essential anatomical resource for developmental biologists needing to know about any aspect of mouse developmental anatomy, as well as for geneticists using the mouse embryo as a model. This book is a companion to Kaufman's The Atlas of Mouse Development and details the developmental anatomy of the early embryo, the transitional tissues, and all the major organ systems. It also includes extensive reference indexes detailing developmental stage criteria, when tissues first appear, and the constituent tissues of embryos at each of the 26 Theiler stages, as well as tissue and author indexes and a glossary. Key features: * Gives anatomical descriptions from oogenesis to birth at a level of detail that often goes beyond that found in the literature. * Provides detailed explanations for geneticists and molecular biologists with limited anatomical background to help them understand the emergence of all the major structures in the mouse embryo. * Contains comprehensive indexes detailing the appearance of over 1000 organs, tissues and their components at different stages of mouse embryogenesis, together with the Theiler developmental stages (1-26) at which each first appears. * Includes comparisons with normal and abnormal human development * Has over 100 clear line diagrams showing mouse developmental anatomy as well as lineage relationships for the major organ systems. This book will be a key reference work for anyone who needs to understand developmental anatomy in normal and mutant mice.

Culture Media, Solutions, and Systems in Human ART

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Publisher : Cambridge University Press
ISBN 13 : 1139917358
Total Pages : 307 pages
Book Rating : 4.1/5 (399 download)

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Book Synopsis Culture Media, Solutions, and Systems in Human ART by : Patrick Quinn

Download or read book Culture Media, Solutions, and Systems in Human ART written by Patrick Quinn and published by Cambridge University Press. This book was released on 2014-03-27 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.

Guidelines for Human Embryonic Stem Cell Research

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Author :
Publisher : National Academies Press
ISBN 13 : 0309096537
Total Pages : 224 pages
Book Rating : 4.3/5 (9 download)

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Book Synopsis Guidelines for Human Embryonic Stem Cell Research by : National Research Council

Download or read book Guidelines for Human Embryonic Stem Cell Research written by National Research Council and published by National Academies Press. This book was released on 2005-09-15 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since 1998, the volume of research being conducted using human embryonic stem (hES) cells has expanded primarily using private funds because of restrictions on the use of federal funds for such research. Given limited federal involvement, privately funded hES cell research has thus far been carried out under a patchwork of existing regulations, many of which were not designed with this research specifically in mind. In addition, hES cell research touches on many ethical, legal, scientific, and policy issues that are of concern to the public. This report provides guidelines for the conduct of hES cell research to address both ethical and scientific concerns. The guidelines are intended to enhance the integrity of privately funded hES cell research by encouraging responsible practices in the conduct of that research.