Laser Desorption Mass Spectrometry For High Throughput Dna Analysis And Its Applications

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Laser Desorption Mass Spectrometry for High Throughput DNA Analysis and Its Applications

Author :
Publisher :
ISBN 13 :
Total Pages : 8 pages
Book Rating : 4.:/5 (683 download)

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Book Synopsis Laser Desorption Mass Spectrometry for High Throughput DNA Analysis and Its Applications by :

Download or read book Laser Desorption Mass Spectrometry for High Throughput DNA Analysis and Its Applications written by and published by . This book was released on 1999 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser desorption mass spectrometry (LDMS) has been developed for DNA sequencing, disease diagnosis, and DNA Fingerprinting for forensic applications. With LDMS, the speed of DNA analysis can be much faster than conventional gel electrophoresis. No dye or radioactive tagging to DNA segments for detection is needed. LDMS is emerging as a new alternative technology for DNA analysis.

Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing

Author :
Publisher :
ISBN 13 :
Total Pages : 11 pages
Book Rating : 4.:/5 (683 download)

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Book Synopsis Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing by :

Download or read book Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing written by and published by . This book was released on 1995 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser desorption mass spectrometry has been considered as a potential new method for fast DNA sequencing. Our approach is to use matrix-assisted laser desorption to produce parent ions of DNA segments and a time-of-flight mass spectrometer to identify the sizes of DNA segments. Thus, the approach is similar to gel electrophoresis sequencing using Sanger's enzymatic method. However, gel, radioactive tagging, and dye labeling are not required. In addition, the sequencing process can possibly be finished within a few hundred microseconds instead of hours and days. In order to use mass spectrometry for fast DNA sequencing, the following three criteria need to be satisfied. They are (1) detection of large DNA segments, (2) sensitivity reaching the femtomole region, and (3) mass resolution good enough to separate DNA segments of a single nucleotide difference. It has been very difficult to detect large DNA segments by mass spectrometry before due to the fragile chemical properties of DNA and low detection sensitivity of DNA ions. We discovered several new matrices to increase the production of DNA ions. By innovative design of a mass spectrometer, we can increase the ion energy up to 45 KeV to enhance the detection sensitivity. Recently, we succeeded in detecting a DNA segment with 500 nucleotides. The sensitivity was 100 femtomole. Thus, we have fulfilled two key criteria for using mass spectrometry for fast DNA sequencing. The major effort in the near future is to improve the resolution. Different approaches are being pursued. When high resolution of mass spectrometry can be achieved and automation of sample preparation is developed, the sequencing speed to reach 500 megabases per year can be feasible.

High-Throughput Mass Spectrometry in Drug Discovery

Author : Chang Liu
Publisher : John Wiley & Sons
ISBN 13 : 1119678463
Total Pages : 516 pages
Book Rating : 4.1/5 (196 download)

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Book Synopsis High-Throughput Mass Spectrometry in Drug Discovery by : Chang Liu

Download or read book High-Throughput Mass Spectrometry in Drug Discovery written by Chang Liu and published by John Wiley & Sons. This book was released on 2023-06-29 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Throughput Mass Spectrometry in Drug Discovery Apply mass spectrometry to every phase of new drug discovery with this cutting-edge guide Mass spectrometry is a technique that identifies and characterizes compounds based on their mass — the fundamental molecular characteristic. It has become an invaluable analytical tool in various disciplines, industries, and research fields. It has become particularly central to new drug discovery and development, which broadly deploys mass spectrometry at every phase. The pharmaceutical industry has become one of the main drivers of technological development in mass spectrometry. High-Throughput Mass Spectrometry in Drug Discovery offers a comprehensive introduction to mass spectrometry and its applications in pharmaceutical discovery. It covers the foundational principles and science of mass spectrometry before moving to specific experimental methods and their applications at various stages of drug discovery. Its thorough treatment and detailed guidance make it an invaluable tool for pharmaceutical research and development. High-Throughput Mass Spectrometry in Drug Discovery readers will also find: Detailed analysis of techniques, including label-free screening, synthetic reaction optimization, and more An authorial team with extensive combined experience in research and industrial applications Technical strategies with the potential to accelerate quantitative bioanalysis in drug discovery High-Throughput Mass Spectrometry in Drug Discovery is essential for analytical, bioanalytical, and medicinal chemists working in the pharmaceutical industry and for any researchers and graduate students interested in drug discovery and development.

Laser Desorption Mass Spectrometry for Fast DNA Analysis

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Publisher :
ISBN 13 :
Total Pages : 9 pages
Book Rating : 4.:/5 (683 download)

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Book Synopsis Laser Desorption Mass Spectrometry for Fast DNA Analysis by :

Download or read book Laser Desorption Mass Spectrometry for Fast DNA Analysis written by and published by . This book was released on 1995 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past few years, major effort has been directed toward developing mass spectrometry to measure biopolymers because of the great potential benefit to biomedical research. Hellenkamp and his co-workers were the first to report that large polypeptide molecules can be ionized and detected without significant fragmentation when a greater number of nicotinic acid molecules are used as a matrix. This method is now well known as matrix-assisted laser desorption/ionization (MALDI). Since then, various groups have reported measurements of very large proteins by MALDI. Reliable protein analysis by MALDI is more or less well established. However, the application of MALDI to nucleic acids analysis has been found to be much more difficult. Most research on the measurement of nucleic acid by MALDI were stimulated by the Human Genome Project. Up to now, the only method for reliable routine analysis of nucleic acid is gel electrophoresis. Different sizes of nucleic acids can be separated in gel medium when a high electric field is applied to the gel. However, the time needed to separate different sizes of DNA segments usually takes from several minutes to several hours. If MALDI can be successfully used for nucleic acids analysis, the analysis time can be reduced to less than I millisecond. In addition, no tagging with radioactive materials or chemical dyes is needed. In this work, we will review recent progress related to MALDI for DNA analysis.

MALDI MS

Author : Franz Hillenkamp
Publisher : John Wiley & Sons
ISBN 13 : 3527673741
Total Pages : 447 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis MALDI MS by : Franz Hillenkamp

Download or read book MALDI MS written by Franz Hillenkamp and published by John Wiley & Sons. This book was released on 2013-10-08 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself. Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with its possibilities and limitations. The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomics), genomics, glycomics and lipidomics. The option of MALDI-MS for the analysis of polymers and small molecules are also covered in separate chapters, while new to this edition is a section devoted to the interplay of MALDI MS and bioinformatics. A much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics.

Chemical Diagnostics

Author : Nelson L.S. Tang
Publisher : Springer
ISBN 13 : 3642399428
Total Pages : 204 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis Chemical Diagnostics by : Nelson L.S. Tang

Download or read book Chemical Diagnostics written by Nelson L.S. Tang and published by Springer. This book was released on 2013-12-06 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next Generation Sequencing: Chemistry, Technology and Applications, by P. Hui Application of Next Generation Sequencing to Molecular Diagnosis of Inherited Diseases, by W. Zhang, H. Cui, L.-J.C. Wong Clinical Applications of the Latest Molecular Diagnostics in Noninvasive Prenatal Diagnosis, by K.C.A. Chan The Role of Protein Structural Analysis in the Next Generation Sequencing Era, by W.W. Yue, D.S. Froese, P.E. Brennan Emerging Applications of Single-Cell Diagnostics, by M. Shirai, T. Taniguchi, H. Kambara Mass Spectrometry in High-Throughput Clinical Biomarker Assays: Multiple Reaction Monitoring, by C.E. Parker, D. Domanski, A.J. Percy, A.G. Chambers, A.G. Camenzind, D.S. Smith, C.H. Borchers Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications, by E.W.Y. Ng, M.Y.M. Wong, T.C.W. Poon Application of Mass Spectrometry in Newborn Screening: About Both Small Molecular Diseases and Lysosomal Storage Diseases, by W.-L. Hwu, Y.-H. Chien, N.-C. Lee, S.-F. Wang, S.-C. Chiang, L.-W. Hsu

Quantitative Analysis of Genetic Variations Using Molecular Affinity and MALDI-TOF Mass Spectrometry

Author : Aana Moon Kim
Publisher :
ISBN 13 :
Total Pages : 39 pages
Book Rating : 4.:/5 (213 download)

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Book Synopsis Quantitative Analysis of Genetic Variations Using Molecular Affinity and MALDI-TOF Mass Spectrometry by : Aana Moon Kim

Download or read book Quantitative Analysis of Genetic Variations Using Molecular Affinity and MALDI-TOF Mass Spectrometry written by Aana Moon Kim and published by . This book was released on 2007 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: A system for DNA quantification is demonstrated utilizing matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a molecular affinity system. MALDI-TOF MS can detect nucleic acids in milliseconds with high resolution, which allows for a high throughput sample analysis. The resulting mass spectra can produce more accurate results than conventional methods based on gel electrophoresis especially when secondary structures exist in the sample DNA molecules. Secondary DNA structures can lead to incomplete separation in the gel and reduce the accuracy in DNA sequence determination. MALDI-TOF MS measures the intrinsic property of DNA mass, and thus allows unambiguous detection of DNA fragments. MALDI TOF MS can be further facilitated by molecular affinity systems such as biotin-streptavidin as they increase the accuracy and throughput of MS. Our approach is based on SPC-SBE, a previously developed method for high throughput genotyping employing molecular affinity, and involves optimization in both sample preparation and MALDI-TOF MS spectral analysis by signal processing. Creating small sample spots with a stainless steel needle to reduce sample crystal heterogeneity improved the DNA quantification system. To further reduce the impact of salt adduct peaks, signal-processing techniques were used for better signal separation. A basic signal processing technique involved ensemble average and ensemble standard deviations of spectra. Ensemble averaging is a simple and powerful signal processing technique to reduce noise. Additionally, the microbead pipette tip device was tested as a new method to rapidly isolate biotinylated DNA fragments and increase throughput of the molecular affinity system. We demonstrate our method provides high quantification accuracy, and therefore can be efficiently used for the application of rapid high-throughput gene expression monitoring.

Laser Mass Spectrometry for DNA Fingerprinting for Forensic Applications

Author :
Publisher :
ISBN 13 :
Total Pages : 10 pages
Book Rating : 4.:/5 (683 download)

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Book Synopsis Laser Mass Spectrometry for DNA Fingerprinting for Forensic Applications by :

Download or read book Laser Mass Spectrometry for DNA Fingerprinting for Forensic Applications written by and published by . This book was released on 1994 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of DNA fingerprinting has become very broad in forensic analysis, patient identification, diagnostic medicine, and wildlife poaching, since every individual's DNA structure is identical within all tissues of their body. DNA fingerprinting was initiated by the use of restriction fragment length polymorphisms (RFLP). In 1987, Nakamura et al. found that a variable number of tandem repeats (VNTR) often occurred in the alleles. The probability of different individuals having the same number of tandem repeats in several different alleles is very low. Thus, the identification of VNTR from genomic DNA became a very reliable method for identification of individuals. DNA fingerprinting is a reliable tool for forensic analysis. In DNA fingerprinting, knowledge of the sequence of tandem repeats and restriction endonuclease sites can provide the basis for identification. The major steps for conventional DNA fingerprinting include (1) specimen processing (2) amplification of selected DNA segments by PCR, and (3) gel electrophoresis to do the final DNA analysis. In this work we propose to use laser desorption mass spectrometry for fast DNA fingerprinting. The process and advantages are discussed.

Advances in Mass Spectrometry

Author : Gareth A.G. Brenton
Publisher : Elsevier
ISBN 13 : 0444515283
Total Pages : 397 pages
Book Rating : 4.4/5 (445 download)

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Book Synopsis Advances in Mass Spectrometry by : Gareth A.G. Brenton

Download or read book Advances in Mass Spectrometry written by Gareth A.G. Brenton and published by Elsevier. This book was released on 2004-06-04 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains contributions based on many of the Invited Lectures given at the 16th triennial International Mass Spectrometry Conference (IMSC) in Edinburgh (September 2003) while the accompanying CD contains the Abstracts of all the accepted papers-Oral and Posters. Traditionally the IMSC has a strong emphasis on the fundamentals and new instrumentation of mass spectrometry and on instrumental developments, and this tradition has continued. There was, however, an equally strong emphasis in Edinburgh on key application areas in mass spectrometry like genomics, proteomics, metabonomics; drug metabolism and bioanalysis, discovery pharma, analysis in pharmaceutical development processes; environmental chemistry, food and nutrition, forensics, and engineering materials. The Invited Lecturers are all international experts. Their articles not only summarise the current state of mass spectrometry in their own areas of expertise but also highlight the current hot topics in mass spectrometry. A must-have for all libraries as an up-to-date summary. - Presents a current summary of mass spectrometry. - Covers both main mass spectrometric applications and developments. - International group of contributors.

Pharmacogenomics

Author : Werner Kalow
Publisher : CRC Press
ISBN 13 : 9781420002461
Total Pages : 434 pages
Book Rating : 4.0/5 (24 download)

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Book Synopsis Pharmacogenomics by : Werner Kalow

Download or read book Pharmacogenomics written by Werner Kalow and published by CRC Press. This book was released on 2001-05-08 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Summarizes the history of, and available applications, techniques, and approaches to, pharmacogenomics--improving efficacy and tolerance to pharmaceutical compounds, evaluating pharmacogenomics on clinical trial design, and increasing the chances of successful clinical trials and patient outcomes. Compares pharmacogenomics to pharmacogenetics, focusing on the essential components that incorporate individual genetic variations to drug regimens resulting in different responses to therapeutics."

Fundamental Studies of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS) and Its Applications to the Analysis of Biological Materials

Download Fundamental Studies of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS) and Its Applications to the Analysis of Biological Materials PDF Online Free

Author : Yongdong Zhu
Publisher :
ISBN 13 :
Total Pages : 364 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Fundamental Studies of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS) and Its Applications to the Analysis of Biological Materials by : Yongdong Zhu

Download or read book Fundamental Studies of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS) and Its Applications to the Analysis of Biological Materials written by Yongdong Zhu and published by . This book was released on 1999 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Analytical Techniques In DNA Sequencing

Author : Brian K. Nunnally
Publisher : CRC Press
ISBN 13 : 9780824753429
Total Pages : 256 pages
Book Rating : 4.7/5 (534 download)

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Book Synopsis Analytical Techniques In DNA Sequencing by : Brian K. Nunnally

Download or read book Analytical Techniques In DNA Sequencing written by Brian K. Nunnally and published by CRC Press. This book was released on 2005-06-23 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: While one marvels at the DNA generated by the Human Genome Project, perhaps just as significant and fascinating is the development of the amazing analytical technology that has permitted us entry into the genomic era and a whole new level of scientific understanding. Analytical Techniques in DNA Sequencing takes a look at the various DNA sequencing techniques that were developed and put to use during and after the Human Genome Project. Nunnally makes a special effort to trace the development of this branch of research since the early days of radioisotope sequencing. Special attention is paid to the breakthrough Sanger Method (1977), as well as to several other techniques that improved the accuracy, improved the detection limits, and in a variety of ways dramatically reduced the time needed to generate a DNA sequence. This comprehensive volume describes the improvements in DNA separation based on advanced capillary electrophoresis and microchip sequencing. It also looks at the use of mass spectrometry and single molecule detection, and takes an in-depth look at the use of various fluorescence methods for the detection of sequencing fragments. One chapter is dedicated to chip-based microfabricated sequencing systems, which could well be the future of DNA sequencing. The book’s ten chapters, authored by a variety of leading experts in the field, include many of the far-reaching applications of DNA sequencing. Entire chapters are dedicated to DNA typing for forensics, a reevaluation of ancient DNA, and genome analysis.

APPLICATIONS OF MASS SPECTROMETRY TO BACTERIAL DIAGNOSTICS: AFFINITY CAPTURE MATRIX ASSISTED LASER DESORPTION

Author : Erin Kaleta
Publisher :
ISBN 13 :
Total Pages : 756 pages
Book Rating : 4.:/5 (752 download)

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Book Synopsis APPLICATIONS OF MASS SPECTROMETRY TO BACTERIAL DIAGNOSTICS: AFFINITY CAPTURE MATRIX ASSISTED LASER DESORPTION by : Erin Kaleta

Download or read book APPLICATIONS OF MASS SPECTROMETRY TO BACTERIAL DIAGNOSTICS: AFFINITY CAPTURE MATRIX ASSISTED LASER DESORPTION written by Erin Kaleta and published by . This book was released on 2011 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation presents the application of mass spectrometry to the detection and characterization of microorganisms based on biomarker identification and DNA analysis. Two major topics are covered: affinity capture mass spectrometry using immunoassay methods and methods involving insertion of membrane receptors into polymerized planar supported lipid bilayers; and the application of mass spectrometry for use in clinical microbiology for the identification of microorganisms causing bloodstream infections. Affinity capture mass spectrometry on immunoassay-based platforms studied the capture of Protein A from Staphylococcus aureus, demonstrating capture that is both selective and sensitive. Experiments illustrated successful capture from a purified source and cell lysates. Affinity capture using receptors inserted into polymerized lipid bilayers was also performed using GM1 and cholera toxin subunit B, demonstrating the enhanced stability offered by polymerizing the lipid bilayers such that direct ionization could be performed. Detection of protein binding was achieved with mass spectrometry at low molar ratios of receptor, and enzymatic digestion experiments on the protein retained at the surface illustrated the ability to characterize the protein ligand bound, lending support to using this technique for reverse pharmacological applications. Lastly, experiments demonstrated that affinity capture of surface-bound proteins can also be used to extract cells from complex mixture prior to the polymerase chain reaction, illustrating utility as a pre-treatment for detecting microorganisms in blood samples. Mass spectrometry was applied to detection of microorganisms from blood culture bottles collected from patients with bloodstream infections. Polymerase chain reaction electrospray ionization and whole cell matrix-assisted laser desorption/ionization mass spectrometry were used to characterize hematopathogens. High diagnostic accuracy was demonstrated with respect to culture-based testing and these two platforms were compared considering accuracy in identification, time to result, and cost benefit analysis. The experiments presented here cover a broad range of detection strategies for identifying proteins and microorganisms. The affinity capture techniques describe the first application of peptide capture and polymerized bilayers for mass spectrometric analysis, and the clinical mass spectrometry work demonstrates validation of two emerging techniques and the first comparative study on both platforms simultaneously. All research presented here demonstrates promise for application of mass spectrometry in diagnostic biology.

High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001

Download High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 5 pages
Book Rating : 4.:/5 (684 download)

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Book Synopsis High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 by :

Download or read book High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 written by and published by . This book was released on 2001 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.

Studies on Surface-Assisted Laser Desorption/Ionization and Its Analytical Application in Imaging Mass Spectrometry

Author : Ho-Wai Tang
Publisher :
ISBN 13 : 9781361294376
Total Pages : pages
Book Rating : 4.2/5 (943 download)

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Book Synopsis Studies on Surface-Assisted Laser Desorption/Ionization and Its Analytical Application in Imaging Mass Spectrometry by : Ho-Wai Tang

Download or read book Studies on Surface-Assisted Laser Desorption/Ionization and Its Analytical Application in Imaging Mass Spectrometry written by Ho-Wai Tang and published by . This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Studies on surface-assisted laser desorption/ionization and its analytical application in imaging mass spectrometry" by Ho-wai, Tang, 鄧浩維, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) is an analytical technique enabling direct chemical analysis of solid samples. Analytes could be desorbed/ionized upon nitrogen laser irradiation from a SALDI substrate-coated sample, then analyzed by MS. The substrate is involved in the transfer of laser energy to the analytes, and eventually assists the desorption/ionization of analytes. The analytical performance of SALDI-MS, such as detection sensitivity, is dependent on different parameters of the substrate, such as size, morphology and form. In this thesis, the effects of several substrate parameters on the SALDI process were investigated. SALDI-MS based Imaging Mass Spectrometry (IMS) method was also developed using efficient SALDI substrate identified in the fundamental studies. IMS is a chemical-specific mapping technique which allows parallel mapping of multiple analytes in solid samples. The desorption mechanism of SALDI is investigated using two groups of substrate, the carbon allotropes and the noble metal nanoparticles. Ion desorption efficiency and internal energy transfer were probed and correlated in carbon-based SALDI. It was found that the ion desorption efficiency and internal energy transfer was in opposite order. Substrate that transferred more internal energy to ions did not show higher ion desorption efficiency. This result could not be explained by the Thermal Desorption model which was a generally believed mechanism of the SALDI desorption process. A non-thermal model, the Phase Transition model is proposed to account for the SALDI desorption process. The Phase Transition model suggests that the substrate is melted/ restructured upon laser irradiation, and this will assist ion desorption. The Phase Transition model is supported by the morphological change of carbon substrates after SALDI and high initial velocity of ions desorbed by carbon-based SALDI (> 1,000 ms-1). SALDI-MS is useful for small molecule analysis due to the relatively clean background in the low mass region. SALDI-IMS is developed and applied to the imaging of spatial distribution of small molecules in forensic and biological samples. Gold nanoparticles (AuNPs) was selected as the substrate from several other noble metal NPs. A solvent-free method, argon ion sputtering, was employed for coating AuNPs on sample surface prior to SALDI-IMS analysis. Fine details of the samples, such as the fine pattern of latent fingerprints and handwriting on questioned documents can be preserved and imaged reliably by avoiding the use of solvent. Fatty acids, drugs and ink components can be imaged in forensic samples including latent fingerprints, banknotes and checks. The solvent-free SALDI-IMS method was also applied to image the distribution of metabolites in intact animal tissues. Spatial distributions of neurotransmitters, nucleobases and fatty acids can be imaged from mouse brain and tumor tissue sections. DOI: 10.5353/th_b4714555 Subjects: Mass spectrometry Imaging systems

Time-of-flight Mass Spectrometry

Author : Robert J. Cotter
Publisher :
ISBN 13 :
Total Pages : 360 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Time-of-flight Mass Spectrometry by : Robert J. Cotter

Download or read book Time-of-flight Mass Spectrometry written by Robert J. Cotter and published by . This book was released on 1997 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the basic principles of time-of-flight (TOF) mass spectrometry with a strong emphasis on applications in biological research. It describes many innovative techniques, including orthogonal extraction, post source decay, and delayed extraction, and surveys ionization techniques used on TOF mass spectrometers such as electron impact, plasma desorption, SIMS, FAB, laser desorption, MALDI, and electrospray. The book features chapters on applications to peptides/proteins, oligonucleotides, and other biological macromolecules, and examines how techniques might be used in sequencing the human genome.

Automation in Proteomics and Genomics

Author : Gil Alterovitz
Publisher : John Wiley & Sons
ISBN 13 : 9780470741177
Total Pages : 340 pages
Book Rating : 4.7/5 (411 download)

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Book Synopsis Automation in Proteomics and Genomics by : Gil Alterovitz

Download or read book Automation in Proteomics and Genomics written by Gil Alterovitz and published by John Wiley & Sons. This book was released on 2009-03-16 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade DNA sequencing costs have decreased over a magnitude, largely because of increasing throughput by incremental advances in tools, technologies and process improvements. Further cost reductions in this and in related proteomics technologies are expected as a result of the development of new high-throughput techniques and the computational machinery needed to analyze data generated. Automation in Proteomics & Genomics: An Engineering Case-Based Approach describes the automation technology currently in the areas of analysis, design, and integration, as well as providing basic biology concepts behind proteomics and genomics. The book also discusses the current technological limitations that can be viewed as an emerging market rather than a research bottleneck. Topics covered include: molecular biology fundamentals: from ‘blueprint’ (DNA) to ‘task list’ (RNA) to ‘molecular machine’ (protein); proteomics methods and technologies; modelling protein networks and interactions analysis via automation: DNA sequencing; microarrays and other parallelization technologies; protein characterization and identification; protein interaction and gene regulatory networks design via automation: DNA synthesis; RNA by design; building protein libraries; synthetic networks integration: multiple modalities; computational and experimental methods; trends in automation for genomics and proteomics new enabling technologies and future applications Automation in Proteomics & Genomics: An Engineering Case-Based Approach is an essential guide to the current capabilities and challenges of high-throughput analysis of genes and proteins for bioinformaticians, engineers, chemists, and biologists interested in developing a cross-discipline problem-solving based approach to systems biology.