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Interaction Of Escherichia Coli Sigma Factors With Core Rna Polymerase
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Book Synopsis Interaction of Escherichia Coli Sigma Factors with Core RNA Polymerase by : Larry C. Anthony
Download or read book Interaction of Escherichia Coli Sigma Factors with Core RNA Polymerase written by Larry C. Anthony and published by . This book was released on 2003 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studying the Interaction of the E. Coli Sigma Factors with Core RNA Polymerase Using LRET by : Bryan T. Glaser
Download or read book Studying the Interaction of the E. Coli Sigma Factors with Core RNA Polymerase Using LRET written by Bryan T. Glaser and published by . This book was released on 2008 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies of Interaction Between the Heat Shock Sigma Factor, O23, and Core RNA Polymerase in Escherichia Coli by : Daniel M. Joo
Download or read book Studies of Interaction Between the Heat Shock Sigma Factor, O23, and Core RNA Polymerase in Escherichia Coli written by Daniel M. Joo and published by . This book was released on 1997 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Genetic Study of the Interactions Between the Major Sigma Factor ([sigma]70) and the Heat-shock Specific Sigma Factor ([sigma]32) of RNA Polymerase in Escherichia Coli by : Yan-Ning Zhou
Download or read book A Genetic Study of the Interactions Between the Major Sigma Factor ([sigma]70) and the Heat-shock Specific Sigma Factor ([sigma]32) of RNA Polymerase in Escherichia Coli written by Yan-Ning Zhou and published by . This book was released on 1991 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis An Analysis of the [sigma]-core Interface in E. Coli by : Meghan M. Sharp
Download or read book An Analysis of the [sigma]-core Interface in E. Coli written by Meghan M. Sharp and published by . This book was released on 2001 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Interactions Between the Transcription Factor Crl and Sigma S RNA Polymerase Holoenzyme in Escherichia Coli by :
Download or read book Molecular Interactions Between the Transcription Factor Crl and Sigma S RNA Polymerase Holoenzyme in Escherichia Coli written by and published by . This book was released on 2013 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria must change their cellular composition in response to changing environmental conditions and stress. They implement these changes by altering gene expression and/or protein activity. Central to changes in gene expression during stress responses is regulation of transcription. In bacteria, transcription is directed by a suite of RNA polymerase (RNAP) holoenzymes, comprised of a core enzyme, possessing the catalytic activity for RNA synthesis, and one of a collection of transcription initiation factors, known as Ï3 factors, which recognizes promoter DNA sequences upstream of genes. Increasing the formation of a particular type of RNAP holoenzyme by switching the Ï3 factor will lead to expression of its cognate regulon. My thesis research focuses on deciphering the complex molecular interactions between the transcription factor Crl and the general stress response sigma factor, Sigma S, in order to understand Escherichia coli's response to stress at the molecular level. Crl is a small (133 amino acids in E. coli) protein that stimulates transcription mediated by Sigma S. At the outset of my research, little was known about how Crl interacts with the transcription machinery to stimulate Sigma S-mediated transcription. I used a combination of biochemical, genetic, and molecular biological techniques in vitro and in vivo to identify key interactions and gain insight into the mechanism of transcriptional regulation by Crl. I identify a key binding determinant (the DPE motif) in Sigma S conserved domain 2 underlying its specific recognition by Crl. I demonstrate directly that Crl requires this determinant for positive regulation of Sigma S-mediated transcription and to enhance Sigma S holoenzyme formation. I made the primary Sigma factor recognizable by Crl by substitution of the DPE motif along with deletion of a large non-conserved region (NCR). I also localized the area of Crl required for the interaction with Sigma S to Crl's conserved central cleft. Finally, I identified an interaction between the Beta prime subunit of core RNAP and Crl, which occurs only in the context of the holoenzyme, that has begun to clarify the mechanism by which Crl functions as a Sigma S-specific RNAP holoenzyme assembly factor.
Book Synopsis Diagnostic Molecular Biology by : Chang-Hui Shen
Download or read book Diagnostic Molecular Biology written by Chang-Hui Shen and published by Elsevier. This book was released on 2023-06-29 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)
Book Synopsis Localization and Characterization of a Sigma Subunit-binding Site on the Echerichia Coli RNA Polymerase Core Enzyme by : Michael Arthur
Download or read book Localization and Characterization of a Sigma Subunit-binding Site on the Echerichia Coli RNA Polymerase Core Enzyme written by Michael Arthur and published by . This book was released on 2000 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure of Bacterial RNA Polymerase Sigma Factors by : Michael Ray Gribskov
Download or read book Structure of Bacterial RNA Polymerase Sigma Factors written by Michael Ray Gribskov and published by . This book was released on 1985 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Interaction of Escherichia Coli RNA Polymerase Holoenzyme Containing Sigma 32 with Promoters for Heat Shock Genes by : Deborah Walker Cowing
Download or read book Interaction of Escherichia Coli RNA Polymerase Holoenzyme Containing Sigma 32 with Promoters for Heat Shock Genes written by Deborah Walker Cowing and published by . This book was released on 1988 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification of Two Regions of the [sigma]70 Subunit of Escherichia Coli RNA Polymerase that are Involved in Promoter Recognition by : Deborah Anne Siegele
Download or read book Identification of Two Regions of the [sigma]70 Subunit of Escherichia Coli RNA Polymerase that are Involved in Promoter Recognition written by Deborah Anne Siegele and published by . This book was released on 1989 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Sigma-F RNA Polymerase of Escherichia Coli by : David Neil Arnosti
Download or read book The Sigma-F RNA Polymerase of Escherichia Coli written by David Neil Arnosti and published by . This book was released on 1989 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA Polymerase and Associated Factors, Part C by : Sankar Adhya
Download or read book RNA Polymerase and Associated Factors, Part C written by Sankar Adhya and published by Elsevier. This book was released on 2003-12-18 with total page 833 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
Book Synopsis Nuclear Magnetic Resonance of Biological Macromolecules, Part C by :
Download or read book Nuclear Magnetic Resonance of Biological Macromolecules, Part C written by and published by Academic Press. This book was released on 2005-04-20 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Book Synopsis Studies of Two Alternate Sigma Factors from Escherichia Coli that Regulate Gene Expression at High Temperature by : James William Erickson
Download or read book Studies of Two Alternate Sigma Factors from Escherichia Coli that Regulate Gene Expression at High Temperature written by James William Erickson and published by . This book was released on 1989 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies on the Function and Regulation of Two Sigma Subunits of Escherichia Coli RNA Polymerase by : Alan Davis Grossman
Download or read book Studies on the Function and Regulation of Two Sigma Subunits of Escherichia Coli RNA Polymerase written by Alan Davis Grossman and published by . This book was released on 1984 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Functional Characterizations of ƠS, an Alternative Sigma Tanscription Factor of Escherichia Coli RNA Polymerase by : Lam Hong Nguyen
Download or read book Functional Characterizations of ƠS, an Alternative Sigma Tanscription Factor of Escherichia Coli RNA Polymerase written by Lam Hong Nguyen and published by . This book was released on 1996 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: