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Improved Analysis Of Dna Short Tandem Repeats With Time Of Flight Mass Spectrometry
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Book Synopsis Improved Analysis of DNA Short Tandem Repeats with Time-of-flight Mass Spectrometry by : John Marshall Butler
Download or read book Improved Analysis of DNA Short Tandem Repeats with Time-of-flight Mass Spectrometry written by John Marshall Butler and published by . This book was released on 2001 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Improved Analysis of DNA Short Tandem Repeats with Time-Of-Flight Mass Spectrometry by : John M. Butler
Download or read book Improved Analysis of DNA Short Tandem Repeats with Time-Of-Flight Mass Spectrometry written by John M. Butler and published by Createspace Independent Pub. This book was released on 2012-07-18 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of DNA typing and its use for human identity testing has revolutionized law enforcement investigations in recent years by allowing forensic laboratories to match suspects with minuscule amounts of biological evidence from a crime scene. Equally important is the use of DNA to exclude suspects who were not involved in a crime or to identify human remains in an accident. The past decade has seen numerous advances in the DNA testing procedures, most notably among them the development of PCR (polymerase chain reaction)-based DNA typing methods. Technologies for measuring DNA variations, both length and sequence polymorphisms, have also advanced rapidly in the past decade. The time needed to determine a sample's DNA profile has dropped from 6–8 weeks to 1–2 days, and with more recent advancements, the time needed to process samples may decrease to as little as a few hours, maybe even a few minutes. Simultaneous with the evolution of DNA markers and technologies embraced by the forensic community has been the acceptance and use of DNA typing information. All 50 States now have laws requiring DNA typing of convicted offenders, typically for violent crimes such as rape or homicide. While the law enforcement community is gearing up to gather millions of DNA samples from convicted felons, the DNA typing technology needs improvement. Large backlogs of samples exist today due to the high cost of performing the DNA testing and limited capabilities in forensic laboratories. A need exists for more rapid and cost-effective methods for high-throughput DNA analysis to process samples currently being gathered for large criminal DNA databases around the world. While larger numbers of samples can be processed by increasing the number of laboratory personnel and instruments, the development of high-throughput DNA processing technologies promises to be more cost effective in the long run, especially for the generation of large DNA databases. GeneTrace Systems, Inc., a small biotechnology company located in Alameda, California, has developed high-throughput DNA analysis capabilities using time-of-flight mass spectrometry coupled with parallel sample preparation on a robotic workstation. The GeneTrace technology allows several thousand samples to be processed daily. DNA samples can be analyzed in seconds, rather than minutes or hours, and with improved accuracy compared with conventional electrophoresis methods. Overall, the mass spectrometry method described in this study is two orders of magnitude faster in sample processing time than conventional techniques. This NIJ project was initiated to adapt the GeneTrace technology to human identity DNA markers commonly used by forensic DNA laboratories, specifically short tandem repeat (STR) markers. An extension of the original grant was submitted in December 1997 to fund the development of single nucleotide polymorphism (SNP) markers from mitochondrial DNA and the Y chromosome. Based on the results obtained in this study, the authors believe mass spectrometry can be a useful and effective means for high-throughput DNA analysis, and that it has the capabilities to meet the needs of the forensic DNA community for offender DNA databases. However, due to limited resources and a perceived difficulty to enter the forensic DNA market, GeneTrace made a business decision to not pursue this market. While the STR milestones on the original grant were met, only the initial milestones were achieved on the SNP portion of the NIJ grant because of the premature termination on the part of GeneTrace. GeneTrace Systems, Inc., developed an integrated high-throughput DNA analysis system involving the use of proprietary chemistry, robotic sample manipulation, and time-of-flight mass spectrometry. The purpose of this NIJ project was to apply the GeneTrace technology to improve the analysis of STR markers commonly used in forensic DNA laboratories.
Book Synopsis Improved Analysis of DNA Short Tandem Repeats by : John M. Butler
Download or read book Improved Analysis of DNA Short Tandem Repeats written by John M. Butler and published by . This book was released on 2001-05-01 with total page 75 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA typing and its use for human identity testing has revolutionized law enforcement investigations by allowing forensic labs to match suspects with minuscule amounts of biological evidence from a crime scene. A need exists for more rapid and cost-effective methods for high-throughput DNA analysis to process samples currently being gathered for large criminal DNA databases around the world. This project was initiated to adapt the GeneTrace technology to human identity DNA markers commonly used by forensic DNA labs, specifically short tandem repeat markers. It was determined that mass spectrometry can be a useful and effective means for high-throughput DNA analysis.
Book Synopsis Improved Analysis of DNA Short Tandem Repeats with Time-Of-Flight Mass Spectrometry by : U.s. Department of Justice
Download or read book Improved Analysis of DNA Short Tandem Repeats with Time-Of-Flight Mass Spectrometry written by U.s. Department of Justice and published by CreateSpace. This book was released on 2014-07-25 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of DNA typing and its use for human identity testing has revolutionized law enforcement investigations in recent years by allowing forensic laboratories to match suspects with minuscule amounts of biological evidence from a crime scene. Equally important is the use of DNA to exclude suspects who were not involved in a crime or to identify human remains in an accident. The past decade has seen numerous advances in the DNA testing procedures, most notably among them the development of PCR (polymerase chain reaction)-based DNA typing methods.
Book Synopsis Improved Analysis of DNA Short Tandem Repeats with Time-of-Flight Mass Spectrometry by : John M. Butler
Download or read book Improved Analysis of DNA Short Tandem Repeats with Time-of-Flight Mass Spectrometry written by John M. Butler and published by . This book was released on 2013-06-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advent of DNA typing and its use for human identity testing has revolutionized law enforcement investigations in recent years by allowing forensic laboratories to match suspects with minuscule amounts of biological evidence from a crime scene. Equally important is the use of DNA to exclude suspects who were not involved in a crime or to identify human remains in an accident. The past decade has seen numerous advances in the DNA testing procedures, most notably among them the development of PCR (polymerase chain reaction)-based DNA typing methods. Technologies for measuring DNA variations, both length and sequence polymorphisms, have also advanced rapidly in the past decade. The time needed to determine a sample's DNA profile has dropped from 6-8 weeks to 1-2 days, and with more recent advancements, the time needed to process samples may decrease to as little as a few hours, maybe even a few minutes.
Book Synopsis Advanced Topics in Forensic DNA Typing: Interpretation by : John M. Butler
Download or read book Advanced Topics in Forensic DNA Typing: Interpretation written by John M. Butler and published by Academic Press. This book was released on 2014-07-28 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. - Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence - Worked mixture examples illustrate the impact of different statistical approaches for reporting results - Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011
Book Synopsis A Guide to Forensic DNA Profiling by : Scott Bader
Download or read book A Guide to Forensic DNA Profiling written by Scott Bader and published by John Wiley & Sons. This book was released on 2016-03-08 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increasingly arcane world of DNA profiling demands that those needing to understand at least some of it must find a source of reliable and understandable information. Combining material from the successful Wiley Encyclopedia of Forensic Science with newly commissioned and updated material, the Editors have used their own extensive experience in criminal casework across the world to compile an informative guide that will provide knowledge and thought-provoking articles of interest to anyone involved or interested in the use of DNA in the forensic context. Following extensive introductory chapters covering forensic DNA profiling and forensic genetics, this comprehensive volume presents a substantial breadth of material covering: Fundamental material – including sources of DNA, validation, and accreditation Analysis and interpretation – including, extraction, quantification, amplification and interpretation of electropherograms (epgs) Evaluation – including mixtures, low template, and transfer Applications – databases, paternity and kinship, mitochondrial-DNA, wildlife DNA, single-nucleotide polymorphism, phenotyping and familial searching Court - report writing, discovery, cross examination, and current controversies With contributions from leading experts across the whole gamut of forensic science, this volume is intended to be authoritative but not authoritarian, informative but comprehensible, and comprehensive but concise. It will prove to be a valuable addition, and useful resource, for scientists, lawyers, teachers, criminologists, and judges.
Book Synopsis Forensic DNA Analysis by : Jaiprakash G. Shewale
Download or read book Forensic DNA Analysis written by Jaiprakash G. Shewale and published by CRC Press. This book was released on 2013-08-19 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing, forensic casework, family lineage studies, identification of human remains, and DNA databasing. Forensic DNA Analysis: Current Practices and Emerging Technologies explores the fundamental principles and the application of technologies for each aspect of forensic DNA analysis. The book begins by discussing the value of DNA evidence and how to properly recognize, document, collect, and store it. The remaining chapters examine: The most widely adopted methods and the best practices for DNA isolation from forensic biological samples and human remains Studies carried out on the use of both messenger RNA and small (micro) RNA profiling Real-time polymerase chain reaction (PCR) methods for quantification and assessment of human DNA prior to genotyping Capillary electrophoresis (CE) as a tool for forensic DNA analysis Next-generation short tandem repeat (STR) genotyping kits for forensic applications, the biological nature of STR loci, and Y-chromosome STRs (Y-STRs) Mitochondrial DNA (mtDNA) sequence analysis Single nucleotide polymorphisms (SNPs) and insertion/deletion polymorphisms (indels) in typing highly degraded DNA Deep-sequencing technologies The current state of integrated systems in forensic DNA analysis The book concludes by discussing various aspects of sample-processing training and the entities that provide such training programs. This volume is an essential resource for students, researchers, teaching faculties, and other professionals interested in human identification/forensic DNA analysis.
Book Synopsis Improved Analysis of DNA Short Tandem Repeats with Time-of-flight Mass Spectrometry by : John Marshall Butler
Download or read book Improved Analysis of DNA Short Tandem Repeats with Time-of-flight Mass Spectrometry written by John Marshall Butler and published by . This book was released on 2001 with total page 75 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Forensic Science by : Stuart H. James
Download or read book Forensic Science written by Stuart H. James and published by CRC Press. This book was released on 2005-02-10 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by highly respected forensic scientists and legal practitioners, Forensic Science: An Introduction to Scientific and Investigative Techniques, Second Edition covers the latest theories and practices in areas such as DNA testing, toxicology, chemistry of explosives and arson, and vehicle accident reconstruction. This second edition offers a cutting-edge presentation of criminalistics and related laboratory subjects, including many exciting new features. What's New in the Second Edition New chapter on forensic entomology New chapter on forensic nursing Simplified DNA chapter More coverage of the chemistry of explosives and ignitable liquids Additional information on crime reconstruction Revised to include more investigation in computer forensics Complete revisions of engineering chapters New appendices showing basic principles of physics, math, and chemistry in forensic science More questions and answers in the Instructor's Guide Updated references and cases throughout An extensive glossary of terms
Download or read book MALDI MS written by Franz Hillenkamp and published by John Wiley & Sons. This book was released on 2013-10-08 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself. Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with its possibilities and limitations. The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomics), genomics, glycomics and lipidomics. The option of MALDI-MS for the analysis of polymers and small molecules are also covered in separate chapters, while new to this edition is a section devoted to the interplay of MALDI MS and bioinformatics. A much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics.
Download or read book NCJRS Catalog written by and published by . This book was released on 2002 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Forensic DNA Typing by : John M. Butler
Download or read book Forensic DNA Typing written by John M. Butler and published by Elsevier. This book was released on 2005-02-08 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic DNA Typing, Second Edition, is the only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome. It examines the science of current forensic DNA typing methods by focusing on the biology, technology, and genetic interpretation of short tandem repeat (STR) markers, which encompass the most common forensic DNA analysis methods used today. The book covers topics from introductory level right up to cutting edge research. High-profile cases are addressed throughout the text, near the sections dealing with the science or issues behind these cases. Ten new chapters have been added to accommodate the explosion of new information since the turn of the century. These additional chapters cover statistical genetic analysis of DNA data, an emerging field of interest to DNA research. Several chapters on statistical analysis of short tandem repeat (STR) typing data have been contributed by Dr. George Carmody, a well-respected professor in forensic genetics. Specific examples make the concepts of population genetics more understandable. This book will be of interest to researchers and practitioners in forensic DNA analysis, forensic scientists, population geneticists, military and private and public forensic laboratories (for identifying individuals through remains), and students of forensic science. *The only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome*Chapters cover the topic from introductory level right up to "cutting edge" research*High-profile cases are addressed throughout the book, near the sections dealing with the science or issues behind these cases*NEW TO THIS EDITION: D.N.A. Boxes--boxed "Data, Notes & Applications" sections throughout the book offer higher levels of detail on specific questions
Book Synopsis Integrated Biochips for DNA Analysis by : Robin Liu
Download or read book Integrated Biochips for DNA Analysis written by Robin Liu and published by Springer Science & Business Media. This book was released on 2008-12-03 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this book is to provide up-to-date coverage of some of the emerging developments in the field of integrated DNA biochips. It will prove a useful source of information for researchers in the field and for those who are just entering the field of biochip research.
Book Synopsis Mass Spectrometry in Cancer Research by : John Roboz
Download or read book Mass Spectrometry in Cancer Research written by John Roboz and published by CRC Press. This book was released on 2002-03-25 with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer research is becoming multidisciplinary. The complex structural and therapeutic problems require synergistic approaches employing an assortment of biochemical manipulations, chromatographic or electrophoretic separations, sequencing strategies, and more and more mass spectrometry. Mass Spectrometry in Cancer Research provides a broad
Book Synopsis Forensic Plant Science by : Jane H Bock
Download or read book Forensic Plant Science written by Jane H Bock and published by Academic Press. This book was released on 2015-11-17 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forensic botany is the application of plant science to the resolution of legal questions. A plant's anatomy and its ecological requirements are in some cases species specific and require taxonomic verification; correct interpretation of botanical evidence can give vital information about a crime scene or a suspect or victim. The use of botanical evidence in legal investigations in North America is relatively recent. The first botanical testimony to be heard in a North American court concerned the kidnapping and murder of Charles Lindbergh's baby boy and the conviction of Bruno Hauptmann in 1935. Today, forensic botany encompasses numerous subdisciplines of plant science, such as plant anatomy, taxonomy, ecology, palynology, and diatomology, and interfaces with other disciplines, e.g., molecular biology, limnology and oceanography.Forensic Plant Science presents chapters on plant science evidence, plant anatomy, plant taxonomic evidence, plant ecology, case studies for all of the above, as well as the educational pathways for the future of forensic plant science. - Provides techniques, collection methods, and analysis of digested plant materials - Shows how to identify plants of use for crime scene and associated evidence in criminal cases - The book's companion website: http://booksite.elsevier.com/9780128014752, will host a microscopic atlas of common food plants
Book Synopsis The Encyclopedia of Mass Spectrometry by : Michael L. Gross
Download or read book The Encyclopedia of Mass Spectrometry written by Michael L. Gross and published by . This book was released on 2003 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt:
