Immunochemistry

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Handbook of Immunochemistry

Author : Miroslav Ferencik
Publisher : Springer Science & Business Media
ISBN 13 : 9401115524
Total Pages : 529 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Handbook of Immunochemistry by : Miroslav Ferencik

Download or read book Handbook of Immunochemistry written by Miroslav Ferencik and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.

Immunohistochemistry and Immunocytochemistry

Author : Simon Renshaw
Publisher : John Wiley & Sons
ISBN 13 : 1118717775
Total Pages : 258 pages
Book Rating : 4.1/5 (187 download)

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Book Synopsis Immunohistochemistry and Immunocytochemistry by : Simon Renshaw

Download or read book Immunohistochemistry and Immunocytochemistry written by Simon Renshaw and published by John Wiley & Sons. This book was released on 2017-02-06 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.

Immunochemistry of Solid-Phase Immunoassay

Author : John E. Butler
Publisher : CRC Press
ISBN 13 : 9780849353949
Total Pages : 334 pages
Book Rating : 4.3/5 (539 download)

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Book Synopsis Immunochemistry of Solid-Phase Immunoassay by : John E. Butler

Download or read book Immunochemistry of Solid-Phase Immunoassay written by John E. Butler and published by CRC Press. This book was released on 1991-07-23 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.

Basic Exercises in Immunochemistry

Author : A. Nowotny
Publisher : Springer Science & Business Media
ISBN 13 : 3642673562
Total Pages : 328 pages
Book Rating : 4.6/5 (426 download)

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Book Synopsis Basic Exercises in Immunochemistry by : A. Nowotny

Download or read book Basic Exercises in Immunochemistry written by A. Nowotny and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Methods in Immunology and Immunochemistry

Author : Curtis Alvin Williams
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (67 download)

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Book Synopsis Methods in Immunology and Immunochemistry by : Curtis Alvin Williams

Download or read book Methods in Immunology and Immunochemistry written by Curtis Alvin Williams and published by . This book was released on 1967 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Analytical Techniques in Immunochemistry

Author : Terry M. Phillips
Publisher : CRC Press
ISBN 13 : 9780824784775
Total Pages : 370 pages
Book Rating : 4.7/5 (847 download)

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Book Synopsis Analytical Techniques in Immunochemistry by : Terry M. Phillips

Download or read book Analytical Techniques in Immunochemistry written by Terry M. Phillips and published by CRC Press. This book was released on 1991-12-23 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores the principles, and presents step-by-step recipes for isolating and measuring biological materials using modern immunochemical methods, including such new procedures as high-performance immunoaffinity chromatography, capillary electrophoresis, and blotting. Also includes clear drawings of

Handbook of Practical Immunohistochemistry

Author : Fan Lin
Publisher : Springer
ISBN 13 : 1493915789
Total Pages : 764 pages
Book Rating : 4.4/5 (939 download)

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Book Synopsis Handbook of Practical Immunohistochemistry by : Fan Lin

Download or read book Handbook of Practical Immunohistochemistry written by Fan Lin and published by Springer. This book was released on 2015-02-21 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

Immunochemistry

Author : Vanoss
Publisher : CRC Press
ISBN 13 : 9780824791230
Total Pages : 1092 pages
Book Rating : 4.7/5 (912 download)

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Book Synopsis Immunochemistry by : Vanoss

Download or read book Immunochemistry written by Vanoss and published by CRC Press. This book was released on 1994-08-24 with total page 1092 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers comprehensive coverage of the chemical and physicochemical aspects of immunological interactions, as well as the molecules and moieties involved in these interactions. It covers in detail the Ag-Ab interaction, including attraction at a distance between epitope and paratope. College or university bookstores may order five or more copies at a special student price, available upon request.

Immunochemistry of Proteins

Author : M. Z. Atassi
Publisher : Springer Science & Business Media
ISBN 13 : 1461329221
Total Pages : 333 pages
Book Rating : 4.4/5 (613 download)

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Book Synopsis Immunochemistry of Proteins by : M. Z. Atassi

Download or read book Immunochemistry of Proteins written by M. Z. Atassi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structural features responsible for the immunogenicity of certain parts of native protein molecules have been of interest to immunochem ists and protein chemists for over three decades. However, until re cently no concerted effort was (or could be) devoted to the elucidation of the complete antigenic structure of a protein. In order for these en deavors to be successful and meaningful, knowledge of both the amino acid sequence and the detailed three-dimensional structure of the protein is necessary. Such information was not available for a protein until early in the 1960s. This and the fact that protein chemistry was not in fact sufficiently developed early in the 1960s to enable the successful unrav eling of the entire antigenic structure of a protein were major reasons for the slow progress in this field. Determination of the antigenic structures of proteins, therefore, posed a chemical challenge of enormous propor tions. For these reasons, many investigators diverted their attention to study of the immunochemistry of amino acid polymers in the hope that the information derived from these systems might prove useful in the understanding of the immunochemistry of proteins. A great many data on these systems were accumulated that have provided valuable infor mation on the immune mechanism. Unfortunately, it has now become clear the information from amino acid polymers has not helped in under standing the immunochemistry of proteins.

Immunochemistry of Proteins

Author : M. Zouhair Atassi
Publisher : Springer Science & Business Media
ISBN 13 : 1461341906
Total Pages : 495 pages
Book Rating : 4.4/5 (613 download)

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Book Synopsis Immunochemistry of Proteins by : M. Zouhair Atassi

Download or read book Immunochemistry of Proteins written by M. Zouhair Atassi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structural features responsible for the immunogenicity of certain parts of native protein molecules have been of interest to immunochemists and protein chemists for over three decades. Following the early work of Land steiner in 1942, which showed that peptide fragments from silk fibroin exhibited an inhibitory activity toward the reaction of the protein with its antibodies, fragments from many other protein systems have been isolated and studied. However, no concerted effort was (or could be) devoted to the elucidation of the complete antigenic structure of a protein. In order for these endeavors to be successful and meaningful, knowledge of both the amino acid sequence and the detailed three-dimensional structure of the protein is necessary. Such information was not available for a protein until early in the 1960s. This and the fact that protein chemistry was not in fact sufficiently developed early in the 1960s to enable the successful completion of the entire antigenic structure of a protein were major contributing factors for the slow progress in this field. Determination of the antigenic structures of proteins therefore posed a chemical challenge of enormous proportions. For these reasons, many investigators diverted their attention to study of the immunochemistry of homo- or mixed amino acid polymers in the hope that the information derived from these systems might prove useful in the understanding of the immunochemistry of proteins.

Serology and Immunochemistry of Plant Viruses

Author : M.H.V. Van Regenmortel
Publisher : Elsevier
ISBN 13 : 0323156320
Total Pages : 317 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Serology and Immunochemistry of Plant Viruses by : M.H.V. Van Regenmortel

Download or read book Serology and Immunochemistry of Plant Viruses written by M.H.V. Van Regenmortel and published by Elsevier. This book was released on 2012-12-02 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses. It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical implementation of the various serological techniques. It also considers the problems associated with identification and classification of plant viruses. Organized into 10 chapters, this volume begins with an overview of antigens and antigenic determinants before proceeding with a discussion of the immunochemistry of plant viruses, virus-antibody binding, the role of quaternary structure in antigenicity, and the structure of viral antigenic determinants. The reader is also introduced to the methods and principles of purifying plant viruses, preparation of antisera and purification of antibodies, antigen-antibody interaction, immunochemical techniques used with plant viruses, the role of quaternary structure on viral antigenicity, diagnosis of virus diseases, use of serological criteria for measuring the degree of relationship between viruses, and immunochemical studies of plant viruses. The book includes a bibliography with 1,400 references and a list of all the plant viruses that have been studied by serology. This book will be a useful resource for virologists and plant pathologists, as well as for students and research workers in plant virology, plant pathology, microbiology, and general virology.

Immunochemistry LabFax

Author :
Publisher : Academic Press
ISBN 13 : 0080984436
Total Pages : 273 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Immunochemistry LabFax by :

Download or read book Immunochemistry LabFax written by and published by Academic Press. This book was released on 2012-12-02 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of searching. The authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs. Immunochemistry Labfax is a detailed compendium of essential information - on plasma proteins, immunoglobulin properties and purification, antibody production, labeling and derivatization, plus data on techniques such as ELISA, blotting, and immunolocalization - needed constantly by researchers in any area of immunology and related disciplines.

Immunochemistry Of The Extracellular Matrix

Author : Furthmayr
Publisher : CRC Press
ISBN 13 : 135108187X
Total Pages : 207 pages
Book Rating : 4.3/5 (51 download)

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Book Synopsis Immunochemistry Of The Extracellular Matrix by : Furthmayr

Download or read book Immunochemistry Of The Extracellular Matrix written by Furthmayr and published by CRC Press. This book was released on 2018-01-18 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main purpose of the two volumes on Immunochemistry of the Extracellular Matrix is to describe state of the art methods, which have been proven to provide antibody reagents of defined specificity to collagens as well as other glycoproteins found in association within connective tissue. The isolation and purification of collagens, procollagens, and related connective tissue proteins are described for several tissues, tissue culture cells and species. Immunization with these collagens in laboratory animals yields antibodies with different characteristic specificities: to the procollagen extension fragments, the non-helical segments of the a-chains, to helical and denatured determinants.

Immunochemistry in Clinical Laboratory Medicine

Author : A.M. Ward
Publisher : Springer Science & Business Media
ISBN 13 : 9401165912
Total Pages : 241 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Immunochemistry in Clinical Laboratory Medicine by : A.M. Ward

Download or read book Immunochemistry in Clinical Laboratory Medicine written by A.M. Ward and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid growth of specific protein estimations in the clinical laboratory over the last 10 years has been due to advances both in methodology and in the understanding of the role of the various plasma proteins in health and disease. This expansion has been made possible by the development of both gel phase and fluid phase techniques for the estimation of proteins and the ready availability of antisera to individual plasma proteins. The specificity of the immunological reaction has allowed the more precise identification and estimation of individual plasma proteins than was possible with dye binding or other chemical techniques, but at the same time these methods have introduced other possible errors and pitfalls. Advances in understanding of the structure and function of various plasma proteins has pointed the way to new clinical applications of plasma protein estimation in the diagnosis and monitoring of disease. The Symposium, of which these are the proceedings, was planned to bring together a number of experts in the field to discuss the available methods and their clinical application.

Methods in Immunology and Immunochemistry

Author : Curtis Williams
Publisher : Elsevier
ISBN 13 : 0323148018
Total Pages : 502 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Methods in Immunology and Immunochemistry by : Curtis Williams

Download or read book Methods in Immunology and Immunochemistry written by Curtis Williams and published by Elsevier. This book was released on 2012-12-02 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Immunology and Immunochemistry, Volume I: Preparation of Antigens and Antibodies is aimed to bring together detailed procedures in the preparation of antigens and antibodies. The text also provides a presentation and discussion of these methods. The book covers topics in immunology such as antigens, its kinds, and the preparation and testing of lipids for immunological study. The production of antiserum; the preparation of immunogens; collecting and handling of serum; and immunization procedures are also explained. The book also discusses the purification of antibodies; methods of labeling antigens and antibodies; and the methods used in the studies of the structure of immunoglobulins. The text is recommended for immunologists who would like to know the different procedures and methods involved in immunology as well as the principles behind it. The book will also serve as a guide for medical staff who prepare products related to immunology.

Contemporary Topics in Immunochemistry

Author : F. Inman
Publisher : Springer Science & Business Media
ISBN 13 : 1475713436
Total Pages : 196 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Contemporary Topics in Immunochemistry by : F. Inman

Download or read book Contemporary Topics in Immunochemistry written by F. Inman and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1897, Ehrlich suggested that natural preformed receptors from the surface of cells provided immunity to various chemical substances. Many years later, in 1940, Pauling proffered the concept that antibodies comprised a single poly peptide chain and that each end of the protein could form an antigen-binding site. Burnet tried to explain the diversity of antibody specificity by hypothesizing that it was cell-derived. These hypotheses probably have led to as much or more experimentation and discussion than any other of the many conjectures set forth to explain immunity on a molecular and cellular basis. Extensive investigations, initially stimulated by these propositions, proved Pauling's notion incorrect. In its demise, however, the multichain structure of the immunoglobulins was realized. In retrospect it be comes obvious that Ehrlich's idea, though not correct, was borne of amazing logic and cognition. Expansion of Burnet's theory seems to be occurring presently; much excitement is engendered by the finding of cell-bound immunoglobulin receptors. Ouring the preceding dozen years, immunochemists have accumulated enormous quantities of data. Though there is so much yet to be done, as a result of this research one may now discuss antigen-binding sites in relation to the protein's primary structure. There is even considerable understanding of the cellular assembly of some immunoglobulins. Entire books can be written about the chemistry of antigens and of complement.

Physical and Chemical Methods

Author : Curtis A. Williams
Publisher : Academic Press
ISBN 13 : 1483222993
Total Pages : 480 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Physical and Chemical Methods by : Curtis A. Williams

Download or read book Physical and Chemical Methods written by Curtis A. Williams and published by Academic Press. This book was released on 2014-05-12 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.