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Functional Analysis Of The Rna Polymerase Ii C Terminal Domain Kinase Ctk1 In The Yeast Saccharomyces Cerevisiae
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Book Synopsis Functional Analysis of the RNA Polymerase II C-terminal Domain Kinase Ctk1 in the Yeast Saccharomyces Cerevisiae by : Susanne Röther
Download or read book Functional Analysis of the RNA Polymerase II C-terminal Domain Kinase Ctk1 in the Yeast Saccharomyces Cerevisiae written by Susanne Röther and published by . This book was released on 2007 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Roles of Threonine-4 and Tyrosine-1 of the RNA Polymerase II C-Terminal Domain by : Nathan Michael Yurko
Download or read book The Roles of Threonine-4 and Tyrosine-1 of the RNA Polymerase II C-Terminal Domain written by Nathan Michael Yurko and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Next, using an in vitro kinase assay, we showed Tyr1 phosphorylation on the CTD by MAP kinase Slt2, and in vivo CTD Tyr1 phosphorylation levels changed based on Slt2-associated stress response, as well as a decrease in in vivo Tyr1P-RNAP II from an Slt2 kinase-dead strain. Analysis of termination factors Nrd1 and Rtt103 showed transcription termination defects were likely the result of disruption of the interaction between the CTD interacting domains of these two proteins and the Y1F CTD. Extending this, we found additional disruptions in Slt2 recruitment to chromatin, increasing the depth of our knowledge of the interplay between induction of stress-associated genes, Slt2 function, and Nrd1-mediated termination.
Download or read book 科学衛星計画シンポジウム written by and published by . This book was released on 1965 with total page 87 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Functional Significance of the Carboxyl-terminal Domain of RNA Polymerase IIa/o by : Paul James Laybourn
Download or read book The Functional Significance of the Carboxyl-terminal Domain of RNA Polymerase IIa/o written by Paul James Laybourn and published by . This book was released on 1989 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of RNA Polymerase II CTD Phosphatase in S. Cerevisiae by : Susanne Jutta Hoheisel
Download or read book Regulation of RNA Polymerase II CTD Phosphatase in S. Cerevisiae written by Susanne Jutta Hoheisel and published by . This book was released on 2005 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Transcription and Splicing by : B. D. Hames
Download or read book Transcription and Splicing written by B. D. Hames and published by Oxford University Press, USA. This book was released on 1988 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a co-ordinated review of our present knowledge of eukaryotic RNA synthesis.
Book Synopsis Chemical Genomics Analysis of TFIIH Kinase Function in Saccharomyces Cerevisiae by : Elenita Ilieva Kanin
Download or read book Chemical Genomics Analysis of TFIIH Kinase Function in Saccharomyces Cerevisiae written by Elenita Ilieva Kanin and published by . This book was released on 2007 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA Polymerase II Carboxy-terminal Domain Phosphatase by : Michael S. Kobor
Download or read book RNA Polymerase II Carboxy-terminal Domain Phosphatase written by Michael S. Kobor and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The form of RNA polymerase II (RNAPII) that binds preferentially to promoters is not extensively phosphorylated on the carboxy-terminal heptapeptide repeat domain (CTD) of its largest subunit. The CTD becomes phosphorylated during or shortly after initiation and elongating RNAPII generally has a phosphorylated CTD. Prior to or following transcriptional termination, dephosphorylation of the CTD presumably must occur to regenerate the hypophosphorylated form of RNAPII that is capable of reinitiating transcription. This thesis examines the function of the CTD phosphatase Fcp1p in the yeast 'Saccharomyces cerevisiae'. In chapter 2, it is shown that Fcp1 is an unusual eukaryotic protein phosphatase that is required for dephosphorylation of the CTD 'in vivo ' and for transcription by RNAPII 'in vivo'. These results suggest that Fcp1p is the founding member of a new class of protein phosphatases and acts as a general transcription factor 'in vivo'. In chapter 3, affinity chromatography is used to study the binding of Fcp1p to TFIIB and the RAP74 subunit of TFIIF. Fcp1p binds in a similar way to both of these factors. RAP74 and TFIIB have a short region of homology and amino acid changes in this region affect the binding to Fcp1p. The genes encoding RAP74 and Fcp1p interact 'in vivo'. Fcp1p can activate transcription when artificially tethered to a promoter and this effect is largely dependent on binding to RAP74. In chapter 4, it is shown that yeast strains with mutations in ' fcp1' grow much worse when the gene encoding the major CTD kinase Kin28p is also mutated. In contrast, inactivation of another CTD kinase encoded by the 'SRB10' gene suppresses the temperature-sensitivity and the sensitivity to certain cell cycle checkpoint inducing drugs of ' fcp1' mutant strains. These results therefore suggest that Fcp1p and Srb10p have opposing roles 'in vivo'. In chapter 5, analysis of the phosphorylation state of the CTD reveals that reduced Fcp1p activity results in a increased amount of the largest subunit of RNAPII but this subunit is not incorporated into functional enzyme and is largely degraded at a higher temperature.
Book Synopsis Fundamentals of Chromatin by : Jerry L. Workman
Download or read book Fundamentals of Chromatin written by Jerry L. Workman and published by Springer Science & Business Media. This book was released on 2013-12-04 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: While there has been an increasing number of books on various aspects of epigenetics, there has been a gap over the years in books that provide a comprehensive understanding of the fundamentals of chromatin. Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. Its primary function is to package DNA to fit into the cell, to strengthen the DNA to prevent damage, to allow mitosis and meiosis, and to control the expression of genes and DNA replication. The audience for this book is mainly newly established scientists and graduate students. Rather than going into the more specific areas of recent research on chromatin the chapters in this book give a strong, updated groundwork about the topic. Some the fundamentals that this book will cover include the structure of chromatin and biochemistry and the enzyme complexes that manage it.
Book Synopsis Biochemistry and Molecular Biology by : Robert Brambl
Download or read book Biochemistry and Molecular Biology written by Robert Brambl and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Series The fungi represent a heterogeneous assemblage of eukaryotic microorganisms and have become favored organisms for research at the cellular and molecular level. Such research involvement has been stimulated by interest in the biotechnological application of fungi in processes related to industry, agriculture and ecology. Considering both yeasts and mycelial fungi, The Mycota highlights developments in both basic and applied research and presents an overview of fungal systematics and cell structure. Foremost authorities in research on mycology have been assembled to edit and contribute to the volumes. This Volume The third volume includes: Membrane Systems and Transport, Responses to Physical Stress, Transcription, Chromosome Replication, Metabolic Pathways and Regulation.
Book Synopsis A Functional Analysis of the RNA Polymerase II Large Subunit Carboxy-terminal Domain by : Rob Chapman
Download or read book A Functional Analysis of the RNA Polymerase II Large Subunit Carboxy-terminal Domain written by Rob Chapman and published by . This book was released on 2002 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Expanding the Functional Repertoire of RNA Polymerase II and CTD Kinase I by : Hemali Pritam Phatnani
Download or read book Expanding the Functional Repertoire of RNA Polymerase II and CTD Kinase I written by Hemali Pritam Phatnani and published by . This book was released on 2004 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Functional Analysis of the Yeast Mitochondrial RNA Polymerase by : David Alan Mangus
Download or read book A Functional Analysis of the Yeast Mitochondrial RNA Polymerase written by David Alan Mangus and published by . This book was released on 1994 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Functional Studies of RNA Polymerase II-dependent Transcription in Yeast Saccharomyces Cerevisiae by : Susanna Tronnersjö
Download or read book Functional Studies of RNA Polymerase II-dependent Transcription in Yeast Saccharomyces Cerevisiae written by Susanna Tronnersjö and published by . This book was released on 2006 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Phosphorylation in Human Health by : Cai Huang
Download or read book Protein Phosphorylation in Human Health written by Cai Huang and published by BoD – Books on Demand. This book was released on 2012-09-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: 15 chapters on protein phosphorylation and human health written by expert scientists. Covers most important research hot points, such as Akt, AMPK and mTOR. Bridges the basic protein phosphorylation pathways with human health and diseases. Detailed and comprehensive text with excellent figure illustration.
Book Synopsis Abstracts, 21st Annual Meetings , March 27-April 4, 1992 by :
Download or read book Abstracts, 21st Annual Meetings , March 27-April 4, 1992 written by and published by . This book was released on 1992 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of RNA Polymerase II Transcription Initiation in the Yeast Saccharomyces Cerevisiae by : Rachel Imoberdorf
Download or read book Analysis of RNA Polymerase II Transcription Initiation in the Yeast Saccharomyces Cerevisiae written by Rachel Imoberdorf and published by . This book was released on 2004 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription occurs in a repressive chromatin context. Chromatin modifications are therefore often required to allow transcriptional activation. Here, we addressed the question whether global histone acetylation is involved in the regulation of transcriptional activation of class II promoters. We show that global histone acetylation has a role in activation of RNA polymerase II dependent transcription by modulating the inherent inhibitory effects of chromatin on the formation of the pre-initiation complex. In a second project we have developed a chromatin immunoprecipitation based assay that allows the study of transcriptional reinitiation "in vivo". Our results suggest the formation of a reinitiation scaffold on the GAL1, but not the ACT1 promoter. In a third project assessed whether TFIIB reaches promoters in the form of the holoenzyme "in vivo". Our data indicate that TFIIB does not assemble in a holoenzyme but associates with another, yet unidentified, factor(s) prior to promoter binding.
