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Evidences For The Non Redundant Function Of A Type Proteins Isca1 And Isca2 In Iron Sulfur Cluster Biogenesis
Download Evidences For The Non Redundant Function Of A Type Proteins Isca1 And Isca2 In Iron Sulfur Cluster Biogenesis full books in PDF, epub, and Kindle. Read online Evidences For The Non Redundant Function Of A Type Proteins Isca1 And Isca2 In Iron Sulfur Cluster Biogenesis ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Evidences for the non-redundant function of A-type proteins ISCA1 and ISCA2 in iron-sulfur cluster biogenesis by : Lena Kristina Beilschmidt
Download or read book Evidences for the non-redundant function of A-type proteins ISCA1 and ISCA2 in iron-sulfur cluster biogenesis written by Lena Kristina Beilschmidt and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Les centres fer-soufre (Fe-S) sont des cofacteurs protéiques essentiels qui participent à un nombre important de fonctions cellulaires allant du métabolisme de l'ADN à la respiration mitochondriale. L'assemblage des centres Fe-S et leur insertion dans des protéines acceptrices requièrent l'activité d'une machinerie protéique dédiée. Bien que les protéines de la biogenèse des centres Fe-S soient conservées, plusieurs aspects fonctionnels et mécanistiques restent inconnus. Notre travail de thèse a consisté à caractériser les protéines mammifères de type A, ISCA1 et ISCA2, qui sont impliquées dans la biogenèse mitochondriales des centres Fe-S. En utilisant une approche couplant l'immunoprécipitation avec une analyse protéomique par spectrométrie de masse, plusieurs interactions protéiques d'ISCA1 et ISCA2 ont pu être identifiées. En plus d'une interaction entre ISCA1 et ISCA2, nous avons ainsi montré l'existence d'interactions spécifiques à chacune de ces protéines. Une approche de knockdown dans la souris via l'injection de virus adéno-associés, a permis de montrer l'absence de redondance fonctionnelle entre ISCA1 et ISCA2 puisque seul ISCA1 se trouve être nécessaire dans la maturation d'une catégorie de protéines à centre Fe-S.
Book Synopsis Structure and Function of Iron-sulfur Cluster Biosynthesis Proteins and the Influence of Oxygen Ligation by : Sheref S. Mansy
Download or read book Structure and Function of Iron-sulfur Cluster Biosynthesis Proteins and the Influence of Oxygen Ligation written by Sheref S. Mansy and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Members of the IscU family of proteins are among the most conserved of all protein groups, extending across all three kingdoms of life. IscU is believed to be involved in iron-sulfur cluster delivery to apo iron-sulfur proteins. However, most of the evidence supporting the function of IscU stems from genetic and cellular biological studies. Therefore, we set out to biochemically characterize human, yeast, and prokaryotic IscU proteins. A variety of spectroscopic techniques were used to evaluate IscU including, UV-visible absorption, Mössbauer, near- and far- UV circular dichroism, mass spectrometry, atomic absorption, and nuclear magnetic resonance. Herein we demonstrate that IscU proteins coordinate reductively labile [2Fe-2S]2 centers and are capable of mediating delivery of intact cluster to apo protein targets. Furthermore, extensive structural and dynamic data of a hyperthermophilic homologue, Thermotoga maritima IscU, revealed that IscU adopts a mobile molten globule-like state that is vastly different from the previously identified ferredoxin-like fold that has thus far been characterized for other metallochaperones. Such a dynamic molecule may allow for the flexibility that is necessary for the multiple roles of Fe-S cluster assembly, and recognition and delivery of that cluster to a target protein. Additionally, we utilized X-ray crystallography to elucidate a high resolution structure of an oxygen ligated [4Fe-4S] high potential iron protein.
Book Synopsis Investigations of Cellular [2Fe-2S] and [4Fe-4S] Cluster Biosynthesis and Trafficking by : Amber Lee Hendricks
Download or read book Investigations of Cellular [2Fe-2S] and [4Fe-4S] Cluster Biosynthesis and Trafficking written by Amber Lee Hendricks and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Iron is the second most abundant metal on earth, and therefore it is not surprising that throughout the kingdoms of life iron is a vital metal in biological processes. A common form of iron that is conserved throughout all organisms is the iron-sulfur cluster. Examples include the [2Fe-2S] and [4Fe-4S] clusters that bind to a number of proteins with specific Fe-S cluster binding sites. Such sites normally consist of four cysteine residues, with occasional replacement of Cys with histidine or aspartate. Iron-sulfur clusters have been connected to several functions in the body, including metabolite synthesis, metabolism, DNA synthesis, biodegradation, and isomerization. The biosynthesis and trafficking of [2Fe-2S] clusters has been studied extensively within the mitochondria, but mysteries remain concerning the cellular chemistry of Fe-S clusters, particularly the cellular formation of [4Fe-4S] clusters and the relationship and possible trafficking of [2Fe-2S] clusters between mitochondria and cytosol of the cell. One of the largest families of proteins, the S-adenosylmethionine (SAM) family, binds a [4Fe-4S] cluster. Lipoyl synthase (LIAS) is a member of the radical S-adenosylmethionine (SAM) superfamily that catalyzes the final step of lipoic acid biosynthesis. The enzyme contains two [4Fe-4S] centers (reducing and auxiliary clusters) that promote radical formation and sulfur transfer, respectively. Most information concerning LIAS and its mechanism has been determined from prokaryotic enzymes. We have made progress in expressing, isolating and characterizing human LIAS, its reactivity, and evaluation of natural iron-sulfur (Fe-S) cluster reconstitution mechanisms. Cluster donation by a number of possible cluster donor proteins and heterodimeric complexes has also been evaluated. [2Fe-2S]-Cluster-bound forms of human ISCU and ISCA2 were found capable of reconstituting human LIAS such that complete product turnover was enabled for LIAS, as monitored via a liquid chromatography-mass spectrometry (LC-MS) assay. EPR studies of native LIAS and substituted derivatives that lacked the ability to bind one or the other of LIAS’s two [4Fe-4S] clusters revealed a likely order of cluster addition, with the auxiliary cluster preceding the reducing [4Fe-4S] center. These results detail the trafficking of Fe-S clusters in human cells and highlight differences with respect to bacterial LIAS analogs. Likely in vivo Fe-S cluster donors to LIAS are identified, with possible connections to human disease states, and a mechanistic ordering of [4Fe-4S] cluster reconstitution is evident. Further studies into the trafficking proteins, ISCA1 and ISCA2 also revealed novel cellular pathways with implications for assembly of [4Fe-4S] centers in cluster proteins. While the metabolic enzyme aconitase could not be reconstituted by ISCA1, ISCA2, or an ISCA1/ISCA2 heterodimer, both ISCA1 and ISCA2 could receive cluster from several trafficking proteins, including ISCU, GLRX5, and Nfu, as confirmed using in vitro CD transfer experiments. Aconitase was readily reconstituted by ISCU, GLRX5, and Nfu. Investigation of the Chaetomium thermophilum (C. thermophilum) protein, Atm1p, also provided insight on an ABC transporter protein implicated in transporting a glutathione-bound [2Fe-2S] cluster across the plasma membrane. Atm1 is a protein that is conserved throughout numerous organisms including humans, and has been linked to various disease states; most notably, sideroblastic anemia. By use of site-directed mutagenesis, and substituting multiple residues in the protein connected to ATPase activity and substrate binding, it was found that the protein was still able to transport the [2Fe-2S] glutathione cluster across a proteoliposome, but the ability to bind the [2Fe-2S] cluster impacted the ability to bind and consume ATP, indicating that residues outside the substrate binding pocket are involved in mediating conformational changes between the substrate binding pocket and nucleotide binding domain.
Book Synopsis Hydrogenosomes and Mitosomes: Mitochondria of Anaerobic Eukaryotes by : Jan Tachezy
Download or read book Hydrogenosomes and Mitosomes: Mitochondria of Anaerobic Eukaryotes written by Jan Tachezy and published by Springer. This book was released on 2020-08-23 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Hydrogenosomes and Mitosomes: Mitochondria of Anaerobic Eukaryotes" provides a summary of the current knowledge of these organelles which occur in unicellular, often parasitic organisms, including human pathogens. These organelles exhibit a variety of structures and functions. This work describes properties such as protein import, structure, metabolism, adaptation, proteome and their role in drug activation and resistance. Further topics include organelle evolution and biogenesis.
Book Synopsis Transition Metals in Catalysis by : Silke Leimkühler
Download or read book Transition Metals in Catalysis written by Silke Leimkühler and published by MDPI. This book was released on 2021-03-10 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Iron–sulfur (FeS) centers are essential protein cofactors in all forms of life. They are involved in many key biological processes. In particular, Fe-S centers not only serve as enzyme cofactors in catalysis and electron transfer, they are also indispensable for the biosynthesis of complex metal-containing cofactors. Among these cofactors are the molybdenum (Moco) and tungsten (Wco) cofactors. Both Moco/Wco biosynthesis and Fe-S cluster assembly are highly conserved among all kingdoms of life. After formation, Fe-S clusters are transferred to carrier proteins, which insert them into recipient apo-proteins. Moco/Wco cofactors are composed of a tricyclic pterin compound, with the metal coordinated to its unique dithiolene group. Moco/Wco biosynthesis starts with an Fe-S cluster-dependent step involving radical/S-adenosylmethionine (SAM) chemistry. The current lack of knowledge of the connection of the assembly/biosynthesis of complex metal-containing cofactors is due to the sheer complexity of their synthesis with regard to both the (genetic) regulation and (chemical) metal center assembly. Studies on these metal-cofactors/cofactor-containing enzymes are important for understanding fundamental cellular processes. They will also provide a comprehensive view of the complex biosynthesis and the catalytic mechanism of metalloenzymes that underlie metal-related human diseases.
Book Synopsis Mitochondria and Anaerobic Energy Metabolism in Eukaryotes by : William F. Martin
Download or read book Mitochondria and Anaerobic Energy Metabolism in Eukaryotes written by William F. Martin and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-12-07 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondria are sometimes called the powerhouses of eukaryotic cells, because mitochondria are the site of ATP synthesis in the cell. ATP is the universal energy currency, it provides the power that runs all other life processes. Humans need oxygen to survive because of ATP synthesis in mitochondria. The sugars from our diet are converted to carbon dioxide in mitochondria in a process that requires oxygen. Just like a fire needs oxygen to burn, our mitochondria need oxygen to make ATP. From textbooks and popular literature one can easily get the impression that all mitochondria require oxygen. But that is not the case. There are many groups of organismsm known that make ATP in mitochondria without the help of oxygen. They have preserved biochemical relicts from the early evolution of eukaryotic cells, which took place during times in Earth history when there was hardly any oxygen avaiable, certainly not enough to breathe. How the anaerobic forms of mitochondria work, in which organisms they occur, and how the eukaryotic anaerobes that possess them fit into the larger picture of rising atmospheric oxygen during Earth history are the topic of this book.
Book Synopsis Methods in Methane Metabolism by : Amy Claire Rosenzweig
Download or read book Methods in Methane Metabolism written by Amy Claire Rosenzweig and published by Academic Press. This book was released on 2011 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.
Book Synopsis Nuclear and Electron Relaxation by : Lucia Banci
Download or read book Nuclear and Electron Relaxation written by Lucia Banci and published by Wiley-VCH. This book was released on 1991-11 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with spin relaxation by underlying the similarities between nuclear and electron spins, and provides a clear and unified picture of the behavior of spins in magnetic resonance. It is especially tailored for scientists dealing with chemical applications of relaxation phenomena. It reviews the basic theory together with the mathematical approach and shows how the theory elucidates the structure and dynamic behavior of transition metal complexes, metal clusters and metalloproteins. The complete theory of contrast agents in magnetic resonance imaging is also presented. The book is a unique and valuable guide for anyone working with paramagnetic systems, from physical and inorganic chemists to biophysicists.
Book Synopsis Euglena: Biochemistry, Cell and Molecular Biology by : Steven D. Schwartzbach
Download or read book Euglena: Biochemistry, Cell and Molecular Biology written by Steven D. Schwartzbach and published by Springer. This book was released on 2017-04-19 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This much-needed book is the first definitive volume on Euglena in twenty-fire years, offering information on its atypical biochemistry, cell and molecular biology, and potential biotechnology applications. This volume gathers together contributions from well-known experts, who in many cases played major roles in elucidating the phenomenon discussed. Presented in three parts, the first section of this comprehensive book describes novel biochemical pathways which in some instances have an atypical subcellular localization. The second section details atypical cellular mechanisms of organelle protein import, organelle nuclear genome interdependence, gene regulation and expression that provides insights into the evolutionary origins of eukaryotic cells. The final section discusses how biotechnologists have capitalized on the novel cellular and biochemical features of Euglena to produce value added products. Euglena: Biochemistry, Cell and Molecular Biology will provide essential reading for cell and molecular biologists with interests in evolution, novel biochemical pathways, organelle biogenesis and algal biotechnology. Readers will come away from this volume with a full understanding of the complexities of the Euglena as well as new realizations regarding the diversity of cellular processes yet to be discovered.
Book Synopsis Anaerobic Parasitic Protozoa by : C. Graham Clark
Download or read book Anaerobic Parasitic Protozoa written by C. Graham Clark and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book internationally acclaimed researchers critically review the most important aspects of research on anaerobic parasitic protozoa, providing the first coherent picture of their genomics and molecular biology since the publication of the genomes. Chapters are written from a molecular and genomic perspective and contain speculative models upon which future research efforts can be based. Topics include: the genomes of Entamoeba histolytica, Trichomonas vaginalis, Giardia and other diplomonads; the cytoskeletons of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis; genomic.
Book Synopsis Heterochromatin by : Ram Sagar Verma
Download or read book Heterochromatin written by Ram Sagar Verma and published by Cambridge University Press. This book was released on 1988-05-27 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'The material included in Heterochromatin is impressively comprehensive and provides timely, authoritative information that would otherwise be difficult to obtain.' BioScience
Book Synopsis Bacterial Biogeochemistry by : Tom Fenchel
Download or read book Bacterial Biogeochemistry written by Tom Fenchel and published by Academic Press. This book was released on 1998-06-02 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial Biogeochemistry, Second Edition focuses on bacterial metabolism and its relevance to the environment, including the decomposition of soil, food chains, nitrogen fixation, assimilation and reduction of carbon nitrogen and sulfur, and microbial symbiosis. The scope of the new edition has broadened to provide a historical perspective, and covers in greater depth topics such as bioenergetic processes, characteristics of microbial communities, spacial heterogeneity, transport mechanisms, microbial biofilms, extreme environments and evolution of biogeochemical cycles. Key Features * Provides up-to-date coverage with an enlarged scope, a new historical perspective, and coverage in greater depth of topics of special interest * Covers interactions between microbial processes, atmospheric composition and the earth's greenhouse properties * Completely rewritten to incorporate all the advances and discoveries of the last 20 years
Book Synopsis Mössbauer Spectroscopy and Transition Metal Chemistry by : P. Gütlich
Download or read book Mössbauer Spectroscopy and Transition Metal Chemistry written by P. Gütlich and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two decades have passed since the original discovery of recoilless nuclear gamma resonance by Rudolf Mossbauer; the spectroscopic method based on this resonance effect - referred to as Mossbauer spectroscopy - has developed into a powerful tool in solid-state research. The users are chemists, physicists, biologists, geologists, and scientists from other disciplines, and the spectrum of problems amenable to this method has become extraordinarily broad. In the present volume we have confined ourselves to applications of Mossbauer spectroscopy to the area of transition elements. We hope that the book will be useful not only to non-Mossbauer special ists with problem-Oriented activities in the chemistry and physics of transition elements, but also to those actively working in the field of Mossbauer spectroscopy on systems (compounds as well as alloys) of transition elements. The first five chapters are directed to introducing the reader who is not familiar with the technique to the principles of the recoilless nuclear resonance effect, the hyperfme interactions between nuclei and electronic properties such as electric and magnetic fields, some essential aspects about measurements, and the evaluation of Moss bauer spectra. Chapter 6 deals with the interpretation of Mossbauer parameters of iron compounds. Here we have placed emphasis on the information about the electronic structure, in correlation with quantum chemical methods, because of its importance for chemical bonding and magnetic properties.
Book Synopsis Metalloproteins by : Juan C. Fontecilla-Camps
Download or read book Metalloproteins written by Juan C. Fontecilla-Camps and published by Humana. This book was released on 2014-03-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.
Book Synopsis Physical Methods in Bioinorganic Chemistry by : Lawrence Que
Download or read book Physical Methods in Bioinorganic Chemistry written by Lawrence Que and published by Sterling Publishing Company. This book was released on 2000 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text provides detailed coverage of physical methods used in bioinorganic chemistry. By integrating theory with experimentation, and providing a more biological orientation, the book aims to serve as a major textbook for students of bioinorganic chemistry.
Download or read book Biochemistry written by and published by Springer. This book was released on 1981-12-01 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Power of Bacterial Genetics by : Jonathan R. Beckwith
Download or read book The Power of Bacterial Genetics written by Jonathan R. Beckwith and published by . This book was released on 1992 with total page 852 pages. Available in PDF, EPUB and Kindle. Book excerpt:
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