Author : Bin Zhuang
Publisher : Springer
ISBN 13 : 9811047537
Total Pages : 131 pages
Book Rating : 4.8/5 (11 download)
Book Synopsis Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis by : Bin Zhuang
Download or read book Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis written by Bin Zhuang and published by Springer. This book was released on 2017-07-20 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.