Design Development And Validation Of A Novel Optical Imaging Device For Biomedical Applications

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Design, Development, and Validation of a Novel Optical Imaging Device for Biomedical Applications

Author : Ashley Christine Dacy
Publisher :
ISBN 13 :
Total Pages : 192 pages
Book Rating : 4.:/5 (132 download)

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Book Synopsis Design, Development, and Validation of a Novel Optical Imaging Device for Biomedical Applications by : Ashley Christine Dacy

Download or read book Design, Development, and Validation of a Novel Optical Imaging Device for Biomedical Applications written by Ashley Christine Dacy and published by . This book was released on 2022 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence and luminescence imaging are two promising optical techniques for diagnosing a variety of critical pathologies in vivo, including wound healing, inflammation, and vascular diseases. Despite this, there is a lack of imaging devices in research and commercially available that are capable of these imaging modalities in large animal applications. This work describes the progressive development of several optical imaging devices to fill this void, culminating in a final design with robust fluorescence and luminescence functionality designed for large animal and human applications. These devices are used to gather new data about various physical parameters in small and large animal models. First, a portable imager developed for real-time imaging of cutaneous wounds in research settings is described. The device is demonstrated to have competitive performance with a commercial animal imaging enclosure box setup in beam uniformity and sensitivity. Specifically, the device was used to visualize the bioluminescence associated with increased reactive oxygen species (ROS) activity during the wound healing process in a cutaneous wound inflammation model. In addition, this device was employed to observe the fluorescence associated with the activity of matrix metalloproteinases (MMPs) in a mouse lipopolysaccharide (LPS)-induced infection model. Our results support the use of the portable imager design as a non-invasive and real-time imaging tool to assess the extent of wound inflammation and infection. The second component of this work details the development and characterization of a portable luminescence imaging device for detecting inflammatory responses and infection in skin wounds. This imager was used to quantify in real time the extent of 2D reactive oxygen species (ROS) activity distribution using a porcine wound infection model. The imager was used to successfully visualize ROS-associated luminescent activities in vitro and in vivo. Using a pig full-thickness cutaneous wound model, the luminescence imager was further demonstrated to be capable of detecting the change of ROS activities and their relationship with vasculature in the wound environment. Finally, by analyzing ROS intensity and distribution, an imaging method was developed to distinguish infected from uninfected wounds. These results demonstrate the potential discovery of a distinct ROS pattern between bacteria-infected and control wounds corresponding to the microvasculature. The final piece of this work describes the design, manufacture, and testing of several fluorescence imagers improving on the design developed in the first part of this work, as well as the development of a penultimate combination fluorescence/luminescence imaging device. This device is compared against a robust industry-standard device and found to be suitable for large animal and potential clinical applications.

Novel Optical Endoscopes for Early Cancer Diagnosis and Therapy

Author : Dale Jonathan Waterhouse
Publisher : Springer
ISBN 13 : 3030214818
Total Pages : 142 pages
Book Rating : 4.0/5 (32 download)

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Book Synopsis Novel Optical Endoscopes for Early Cancer Diagnosis and Therapy by : Dale Jonathan Waterhouse

Download or read book Novel Optical Endoscopes for Early Cancer Diagnosis and Therapy written by Dale Jonathan Waterhouse and published by Springer. This book was released on 2019-07-09 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes the design, development, characterisation and clinical translation of three novel devices for optical endoscopic imaging. Over the past decade, rapid innovation in optics and photonics has led to the availability of low-cost and high-performance optical technologies that can be exploited for biomedical applications, but relatively few have been translated into clinic. The work presented outlines for the first time, a comprehensive analysis of the common barriers and unique challenges associated with the translation of optical imaging techniques. To assist developers streamline translation of optical imaging devices in future, a roadmap to clinical translation is outlined, and key translational characteristics are defined. Guided by these, subsequent development of endoscopic devices resulted in preparation and approval of endoscopes for first in human trials in the oesophagus, for early detection of cancer, and in the brain, for delineation of tumour during surgical resection. The thesis culminates in the presentation of results from the first in human use of a compact multispectral endoscope for imaging endogenous tissue contrast in the oesophagus. With continuation of the work as outlined at the end of this thesis, the novel techniques described have the potential to improve the standard of care in their respective indications.

Biomedical Optical Imaging Technologies

Author : Rongguang Liang
Publisher : Springer Science & Business Media
ISBN 13 : 364228390X
Total Pages : 391 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis Biomedical Optical Imaging Technologies by : Rongguang Liang

Download or read book Biomedical Optical Imaging Technologies written by Rongguang Liang and published by Springer Science & Business Media. This book was released on 2012-09-21 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to design of biomedical optical imaging technologies and their applications. The main topics include: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic systems. Each chapter is written by the world leaders of the respective fields, and will cover: principles and limitations of optical imaging technology, system design and practical implementation for one or two specific applications, including design guidelines, system configuration, optical design, component requirements and selection, system optimization and design examples, recent advances and applications in biomedical researches and clinical imaging. This book serves as a reference for students and researchers in optics and biomedical engineering.

Biomedical Optical Imaging

Author : James G. Fujimoto
Publisher : Oxford University Press
ISBN 13 : 0199722293
Total Pages : 435 pages
Book Rating : 4.1/5 (997 download)

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Book Synopsis Biomedical Optical Imaging by : James G. Fujimoto

Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

Optical Imaging for Biomedical and Clinical Applications

Author : Ahmad Fadzil Mohamad Hani
Publisher : CRC Press
ISBN 13 : 1498750389
Total Pages : 441 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis Optical Imaging for Biomedical and Clinical Applications by : Ahmad Fadzil Mohamad Hani

Download or read book Optical Imaging for Biomedical and Clinical Applications written by Ahmad Fadzil Mohamad Hani and published by CRC Press. This book was released on 2017-10-30 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical imaging is a rapidly emerging imaging technique that has been successfjully translated into biomedical applications ranging from clinical diagnosis to molecular biology. This book includes an introductory section to explore various optical imaging devices and their functionality and roles for biomedical applications such as dermatology and ophthalmology. Recent developments as exemplified with the authors research are explored in detail. In depth discussion of other disease conditions and their diagnosis with optical imaging techniques are also covered.

Novel Biomedical Imaging Systems

Author : Yuan Luo
Publisher :
ISBN 13 :
Total Pages : 376 pages
Book Rating : 4.:/5 (659 download)

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Book Synopsis Novel Biomedical Imaging Systems by : Yuan Luo

Download or read book Novel Biomedical Imaging Systems written by Yuan Luo and published by . This book was released on 2008 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overall purpose of the dissertation is to design and develop novel optical imaging systems that require minimal or no mechanical scanning to reduce the acquisition time for extracting image data from biological tissue samples. Two imaging modalities have been focused upon: a parallel optical coherence tomography (POCT) system and a volume holographic imaging system (VHIS). Optical coherence tomography (OCT) is a coherent imaging technique, which shows great promise in biomedical applications. A POCT system is a novel technology that replaces mechanically transverse scanning in the lateral direction with electronic scanning. This will reduce the time required to acquire image data. In this system an array with multiple reduced diameter (15æ̐m) single mode fibers (SMFs) is required to obtain an image in the transverse direction. Each fiber in the array is configured in an interferometer and is used to image one pixel in the transverse direction. A VHIS is based on volume holographic gratings acting as Bragg filters in conjunction with conventional optical imaging components to form a spatial-spectral imaging system. The high angular selectivity of the VHIS can be used to obtain two-dimensional image information from objects without the need for mechanical scanning. In addition, the high wavelength selectivity of the VHIS can provide spectral information of a specific area of the object that is being observed. Multiple sections of the object are projected using multiplexed holographic gratings in the same volume of the Phenanthrenquinone- (PQ- ) doped Poly (methyl methacrylate) (PMMA) recording material.

Further Developments in Scientific Optical Imaging

Author : M Bonner Denton
Publisher : Royal Society of Chemistry
ISBN 13 : 1847550959
Total Pages : 217 pages
Book Rating : 4.8/5 (475 download)

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Book Synopsis Further Developments in Scientific Optical Imaging by : M Bonner Denton

Download or read book Further Developments in Scientific Optical Imaging written by M Bonner Denton and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: The number of researchers using imaging devices in their work continues to increase rapidly. Disciplines including astronomy, biology, chemistry, physics and manufacturers of imaging devices, optical components and complete optical systems are recognising the enormous potential. Further Developments in Scientific Optical Imaging brings together the latest information on commercial and academic research, development and applications in scientific optical imaging, from state-of-the-art devices to exciting explorations in space. Topics range from a new generation of CCDs, through spectroscopic applications of CTDs, to improved image processing and new applications for microscopy and spectroscopy. Experts from around the world provide overviews of important aspects of optical imaging, such as design considerations, device fabrication and integration, and data reduction. Comprehensive and international in coverage, this book will be welcomed by developers, manufacturers and users of this technology in universities, observatories and businesses around the world.

Novel Optical Devices for Biomedical Imaging and Spectroscopy

Author : Chenji Zhang
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (12 download)

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Book Synopsis Novel Optical Devices for Biomedical Imaging and Spectroscopy by : Chenji Zhang

Download or read book Novel Optical Devices for Biomedical Imaging and Spectroscopy written by Chenji Zhang and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: With the growing demand for better medical care worldwide, biomedical imaging and spectroscopy which provide critical information for disease diagnosis and personal health condition have become a focal point. In order to output high quality imaging and spectroscopy results, which is critical for diagnosis accuracy, optical devices have been extensively explored. In this dissertation, I present several novel optical devices for biomedical imaging and spectroscopy. They are all capable of promoting the performance of existing imaging and spectroscopy applications or enabling new applications.In Chapter two, I present a laser based light source which is ultrafast, multi-line, dual polarization, broadly tunable, and power scalable. This light source could be an ideal source for nonlinear optical imaging. We combine soliton self-frequency shift and pulse division method together to achieve these features. Specifically, power scalable is achieved through pulse division and recombination, which provides a general solution for conventional soliton self-frequency shift source whose pulse energy is limited. Also, benefited from the pulse division, divided pulses can be shifted individually, which enables multi-wavelength and dual polarization capability. Simultaneous dual-polarization second-harmonic generation imaging is demonstrated based on these features. In Chapter three, I present a biodegradable step-index optical fiber, which is suitable for light delivery under in vivo condition. The fiber has desirable mechanical and biological properties which are superior to traditional silica optical fiber. This is attributed to the programmable citrate-based material platform we use to fabricate the fiber. The fiber has a 0.4dB/cm loss, with a 1/e length longer than 10cm. By using this fiber, successful light delivery in tissue as well as fluorescence excitation and collection are accomplished, which prove the fibers usefulness for in vivo applications. Further, successful image delivery verifies the fibers ability for image transmission, which can potentially enable endoscopy applications. In Chapter four, I demonstrate two smartphone based detection devices, suitable for spectroscopy applications. Both of them benefit from the smartphone, which is ideal for point-of-care applications. Specifically, we developed a G-Fresnel smartphone based spectrometer. Successful protein concentration measurement using Bradford reagents demonstrates its ability for general spectroscopy applications. In additional to the smartphone spectrometer, we also developed a smartphone chloridometer based on a citrate chloride sensor. Through clinical verification, the device shows high accuracy and broad measurement range, which can potential serve as a device for cystic fibrosis diagnosis.

Optical Imaging in Human Disease and Biological Research

Author : Xunbin Wei
Publisher : Springer Nature
ISBN 13 : 9811576270
Total Pages : 309 pages
Book Rating : 4.8/5 (115 download)

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Book Synopsis Optical Imaging in Human Disease and Biological Research by : Xunbin Wei

Download or read book Optical Imaging in Human Disease and Biological Research written by Xunbin Wei and published by Springer Nature. This book was released on 2021-05-29 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.

System and Process Optimization for Biomedical Optical Imaging

Author : Yehe Liu
Publisher :
ISBN 13 :
Total Pages : 325 pages
Book Rating : 4.:/5 (129 download)

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Book Synopsis System and Process Optimization for Biomedical Optical Imaging by : Yehe Liu

Download or read book System and Process Optimization for Biomedical Optical Imaging written by Yehe Liu and published by . This book was released on 2021 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is widely used in the research community and medical diagnosis. However, many optical imaging techniques in the biomedical field are not optimized. Developing and improving these techniques is often a complex task involving sample preparation, instrument design, and data handling. As a summary of my Ph.D. research, this dissertation discusses the design optimization strategies for biomedical optical imaging applications. It is structured with an introduction discussing the general design consideration and the design process for biomedical optical imaging, followed by detailed discussions of four biomedical optical imaging applications developed during my Ph.D. program. The dissertation has six major chapters. The first chapter summarizes the general technology development processes for biomedical optical imaging applications, It introduces the three main elements of biomedical optical imaging, which include sample preparation, imaging systems, and data handling. Following the introduction, the second chapter discusses the Pocket MUSE project, a smartphone-based fluorescence microscope that costs less than $20 to build. Other highlights of the design include a small footprint, high imaging performance, versatile sample compatibility, and simple sample preparation methods. The third chapter describes the SLIME project, an OCT-based 3D microvascular mapping technique using an injectable scattering contrast agent, developed to study the coronary vascular abnormalities in a quail embryo animal model. This technique features a novel sample preparation strategy, where the PVA-borate cross-linking (slime) chemistry is used to stabilize a low-viscosity scattering contrast agent in situ. The fourth chapter introduces the LIMPID project, a simple optical clearing technique that makes tissues transparent in a single step. This sample preparation technique is designed to enhance the imaging depth for a broad range of optical imaging applications, enabling 3D optical imaging of millimeters-thick tissue samples. The fifth chapter describes the CompassLSM project, a simple yet high-performance light sheet microscope with sub-4-μm high optical sectioning capability in a multi-millimeter large FOV. The high imaging performance is achieved by synchronizing the focus of an axially swept light sheet to the rolling shutter of a CMOS camera. The final chapter provides a summary and conclusions about this dissertation.

Deep Imaging in Tissue and Biomedical Materials

Author : Lingyan Shi
Publisher : Jenny Stanford Publishing
ISBN 13 : 9789814745888
Total Pages : 0 pages
Book Rating : 4.7/5 (458 download)

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Book Synopsis Deep Imaging in Tissue and Biomedical Materials by : Lingyan Shi

Download or read book Deep Imaging in Tissue and Biomedical Materials written by Lingyan Shi and published by Jenny Stanford Publishing. This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of light for probing and imaging biomedical media is promising for the development of safe, noninvasive, and inexpensive clinical imaging modalities with diagnostic ability. The advent of ultrafast lasers has enabled applications of nonlinear optical processes, which allow deeper imaging in biological tissues with higher spatial resolution. This book provides an overview of emerging novel optical imaging techniques, Gaussian beam optics, light scattering, nonlinear optics, and nonlinear optical tomography of tissues and cells. It consists of pioneering works that employ different linear and nonlinear optical imaging techniques for deep tissue imaging, including the new applications of single- and multiphoton excitation fluorescence, Raman scattering, resonance Raman spectroscopy, second harmonic generation, stimulated Raman scattering gain and loss, coherent anti-Stokes Raman spectroscopy, and near-infrared and mid-infrared supercontinuum spectroscopy. The book is a comprehensive reference of emerging deep tissue imaging techniques for researchers and students working in various disciplines.

Optical Methods and Instrumentation in Brain Imaging and Therapy

Author : Steen J. Madsen
Publisher : Springer Science & Business Media
ISBN 13 : 1461449782
Total Pages : 284 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Optical Methods and Instrumentation in Brain Imaging and Therapy by : Steen J. Madsen

Download or read book Optical Methods and Instrumentation in Brain Imaging and Therapy written by Steen J. Madsen and published by Springer Science & Business Media. This book was released on 2012-11-13 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive up-to-date review of optical approaches used in brain imaging and therapy. It covers a variety of imaging approaches including diffuse optical imaging, laser speckle imaging, photoacoustic imaging and optical coherence tomography. A number of laser-based therapeutic techniques are reviewed, including photodynamic therapy, fluorescence guided resection and photothermal therapy. Fundamental principles and instrumentation are discussed for each imaging and therapeutic approach.

BioSensing, Theranostics, and Medical Devices

Author : Vivek Borse
Publisher : Springer Nature
ISBN 13 : 9811627827
Total Pages : 380 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis BioSensing, Theranostics, and Medical Devices by : Vivek Borse

Download or read book BioSensing, Theranostics, and Medical Devices written by Vivek Borse and published by Springer Nature. This book was released on 2021-12-09 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides up-to-date information on the prototypes used to develop medical devices and explains the principles of biosensing and theranostics. It also discusses the development of biosensor and application-orientated design of medical devices. In addition to summarizing the clinical validation of the developed techniques and devices and the regulatory steps involved in their commercialization, the book highlights the latest research and translational technologies toward the development of point-of-care devices in the health care. Lastly, it explores the current opportunities, challenges and provides troubleshooting on the use of biosensors in precision medicine. The book is helpful for researchers and medical professionals working in the field of clinical theranostics, and medical-device development wanting to gain a better understanding into the principles and processes involved in the development of biosensors.

Novel Optical Instrumentation for Biomedical Applications III

Author :
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (746 download)

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Book Synopsis Novel Optical Instrumentation for Biomedical Applications III by :

Download or read book Novel Optical Instrumentation for Biomedical Applications III written by and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Translational Multimodality Optical Imaging

Author : Fred S. Azar
Publisher : Artech House
ISBN 13 : 1596933089
Total Pages : 431 pages
Book Rating : 4.5/5 (969 download)

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Book Synopsis Translational Multimodality Optical Imaging by : Fred S. Azar

Download or read book Translational Multimodality Optical Imaging written by Fred S. Azar and published by Artech House. This book was released on 2008 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: Supported with 119 illustrations, this milestone work discusses key optical imaging techniques in self-contained chapters; describes the integration of optical imaging techniques with other modalities like MRI, X-ray imaging, and PET imaging; provides a software platform for multimodal integration; presents cutting-edge computational and data processing techniques that ensure rapid, cost-effective, and precise quantification and characterization of the clinical data; covers advances in photodynamic therapy and molecular imaging, and reviews key clinical studies in optical imaging along with regulatory and business issues.

Advances in Optical Imaging for Clinical Medicine

Author : Nicusor Iftimia
Publisher : Wiley
ISBN 13 : 9780470619094
Total Pages : 537 pages
Book Rating : 4.6/5 (19 download)

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Book Synopsis Advances in Optical Imaging for Clinical Medicine by : Nicusor Iftimia

Download or read book Advances in Optical Imaging for Clinical Medicine written by Nicusor Iftimia and published by Wiley. This book was released on 2011-02-15 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides students, teachers, researchers and clinicians with a strong and established source of information on advanced optical technologies that show real promise of being translated to clinical use.

Advanced Biophotonics

Author : Ruikang K. Wang
Publisher : CRC Press
ISBN 13 : 1439895813
Total Pages : 735 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Advanced Biophotonics by : Ruikang K. Wang

Download or read book Advanced Biophotonics written by Ruikang K. Wang and published by CRC Press. This book was released on 2013-07-23 with total page 735 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and applications of biophotonics imaging. Gathering research otherwise scattered in numerous physical, chemical, biophysical, and biomedical journals, the book helps researchers, bioengineers, and medical doctors understand major recent bioimaging technologies and the underlying biophotonics science. Well-known international experts explore a variety of "hot" biomedical optics and biophotonics problems, including the use of photoacoustic imaging to investigate the molecular and cellular processes in living systems. The book also covers Monte Carlo modeling, tissue optics and tissue optical clearing, nonlinear optical microscopy, various aspects of optical coherence tomography, multimodal tomography, adaptive optics, and signal imaging. With 58 color images, this book represents a valuable contribution to the biomedical and biophotonics literature. Designed for researchers and practitioners in biophotonics, the book is also a useful resource for scientists in laser physics and technology, fiber optics, spectroscopy, materials science, biology, and medicine as well as students studying biomedical physics and engineering, biomedical optics, and biophotonics.