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Characterization Of The Mammalian Dna Polymerase Genes And Enzymes Annual Progress Report
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Book Synopsis Characterization of the Mammalian DNA Polymerase Gene(s) and Enzyme(s). Annual Progress Report by :
Download or read book Characterization of the Mammalian DNA Polymerase Gene(s) and Enzyme(s). Annual Progress Report written by and published by . This book was released on 1994 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: Consistent with the long term goal of our research to understand the nature of the key enzymes in eukaryotic DNA replication we have characterized the properties of the wild type DNA polymerases of the [alpha]-family and their mutants. We have also provided evidence for the role of aphidicolin in the elongation process of the in vivo DNA replication in eukaryotic cells. We also developed a technology for planned prep from a large numbers of clones for direct screening by size or restriction digestion in order to facilitate our goals to clone the DNA polymerase gene.
Book Synopsis Characterization of the Mammalian DNA Polymerase Gene and Protein. Annual Progress Report by :
Download or read book Characterization of the Mammalian DNA Polymerase Gene and Protein. Annual Progress Report written by and published by . This book was released on 1992 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have purified and characterized at least three DNA polymerases from Chinese hamster ovary (CHO) cell line Kl in order to evaluate the roles of different polymerases in eukaryotic DNA replication. Pol [alpha] was the most abundant among different polymerase activities and it was neutralized by a monoclonal antibody raised against human pol [alpha]. Pol {var_epsilon} was separated from pol [alpha] and pol [delta] activities using DEAE Sephacell, phosphocellulose and hydroxylapatite columns. The enzyme proved to be sensitive to aphidicolin and carbonyldiphosphonate and was not stimulated by PCNA- Pol [delta] was the least abundant among the three enzymes. It was sensitive to aphidicolin and carbonyidiphosphonate and was stimulated by PCNA. it had a preference for template/primer poly (dA-dT). Based on DNA sequence data of different eukaryotic polymerases PCR primers complementary to two neighboring synthesized. In PCR experiments several products were obtained which are assumed to be specific for the CHO polymerases. We have also analyzed a large number of aphidicolin resistant mutants of CHO to identify mutants with altered DNA polymerases.
Download or read book Energy Research Abstracts written by and published by . This book was released on 1993 with total page 762 pages. Available in PDF, EPUB and Kindle. Book excerpt: Semiannual, with semiannual and annual indexes. References to all scientific and technical literature coming from DOE, its laboratories, energy centers, and contractors. Includes all works deriving from DOE, other related government-sponsored information, and foreign nonnuclear information. Arranged under 39 categories, e.g., Biomedical sciences, basic studies; Biomedical sciences, applied studies; Health and safety; and Fusion energy. Entry gives bibliographical information and abstract. Corporate, author, subject, report number indexes.
Book Synopsis Government Reports Announcements & Index by :
Download or read book Government Reports Announcements & Index written by and published by . This book was released on 1996 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :National Cancer Institute (U.S.). Division of Cancer Research Resources and Centers Publisher : ISBN 13 : Total Pages :204 pages Book Rating :4.:/5 (3 download)
Book Synopsis Annual Report - National Cancer Institute, Division of Cancer Research Resources and Centers by : National Cancer Institute (U.S.). Division of Cancer Research Resources and Centers
Download or read book Annual Report - National Cancer Institute, Division of Cancer Research Resources and Centers written by National Cancer Institute (U.S.). Division of Cancer Research Resources and Centers and published by . This book was released on 1976 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nuclear Science Abstracts written by and published by . This book was released on 1976 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis ERDA Research Abstracts by : United States. Energy Research and Development Administration
Download or read book ERDA Research Abstracts written by United States. Energy Research and Development Administration and published by . This book was released on 1976 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis ERDA Energy Research Abstracts by : United States. Energy Research and Development Administration
Download or read book ERDA Energy Research Abstracts written by United States. Energy Research and Development Administration and published by . This book was released on 1976-05 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Human Dna Polymerases: Biology, Medicine And Biotechnology by : Giovanni Maga
Download or read book Human Dna Polymerases: Biology, Medicine And Biotechnology written by Giovanni Maga and published by World Scientific. This book was released on 2017-11-10 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, next generation sequencing, nucleic acids based diagnostics and in techniques to analyze ancient and otherwise damaged DNA (e.g. for forensic applications). Moreover, some diseases are related to DNA polymerase defects and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. This book focuses on (i) biology of DNA polymerases, (ii) medical aspects of DNA polymerases and (iii) biotechnological applications of DNA polymerases. It is intended for a wide audience from basic scientists, to diagnostic laboratories, to companies and to clinicians, who seek a better understanding and the practical use of these fascinating enzymes.
Book Synopsis Annual Report by : National Cancer Institute (U.S.). Division of Cancer Etiology
Download or read book Annual Report written by National Cancer Institute (U.S.). Division of Cancer Etiology and published by . This book was released on 1990 with total page 758 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Government Reports Annual Index by :
Download or read book Government Reports Annual Index written by and published by . This book was released on 1993 with total page 1834 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Annual Report - National Cancer Institute by : National Cancer Institute (U.S.)
Download or read book Annual Report - National Cancer Institute written by National Cancer Institute (U.S.) and published by . This book was released on 1976 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nuclear Science Abstracts written by and published by . This book was released on 1968-07 with total page 1096 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Energy Research Abstracts written by and published by . This book was released on 1988 with total page 908 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes all works deriving from DOE, other related government-sponsored information and foreign nonnuclear information.
Author :United States. Energy Research and Development Administration. Technical Information Center Publisher : ISBN 13 : Total Pages :724 pages Book Rating :4.3/5 (129 download)
Book Synopsis ERDA Energy Research Abstracts by : United States. Energy Research and Development Administration. Technical Information Center
Download or read book ERDA Energy Research Abstracts written by United States. Energy Research and Development Administration. Technical Information Center and published by . This book was released on 1976 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Annual Progress Report - The University of Michigan, Dental Research Institute by : University of Michigan. Dental Research Institute
Download or read book Annual Progress Report - The University of Michigan, Dental Research Institute written by University of Michigan. Dental Research Institute and published by . This book was released on 1980 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Annual Reports of the Research Institute for Microbial Diseases, Osaka University by : Ōsaka Daigaku. Biseibutsubyō Kenkyūjo
Download or read book Annual Reports of the Research Institute for Microbial Diseases, Osaka University written by Ōsaka Daigaku. Biseibutsubyō Kenkyūjo and published by . This book was released on 2002 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
