Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Cell Cycle Analysis Of 3 Hydroxy 3 Methylglutaryl Coenzyme A Reductase In Cho And Ut2 Cell Lines
Download Cell Cycle Analysis Of 3 Hydroxy 3 Methylglutaryl Coenzyme A Reductase In Cho And Ut2 Cell Lines full books in PDF, epub, and Kindle. Read online Cell Cycle Analysis Of 3 Hydroxy 3 Methylglutaryl Coenzyme A Reductase In Cho And Ut2 Cell Lines ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Analysis of a CHO Cell Line that Expresses a Peroxisomal 3-Hydroxy-3-methylglutaryl-CoA Reductase by : William Engfelt
Download or read book Analysis of a CHO Cell Line that Expresses a Peroxisomal 3-Hydroxy-3-methylglutaryl-CoA Reductase written by William Engfelt and published by . This book was released on 1998 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase is present not only in the endoplasmic reticulum (ER) but also in the peroxisomes. To date no information is available regarding the function of the peroxisomal HMG-CoA reductase in cholesterol/isoprenoid metabolism and the structure of the peroxisomal HMG-CoA reductase has yet to be determined. We have identified a mammalian cell line that expresses only one HMG-CoA reductase protein and which is localized exclusively to peroxisomes to facilitate our studies on the function, regulation, and structure of the peroxisomal HMG-CoA reductase. This cell line was obtained by growing UT2 cells (which lack the ER HMG-CoA reductase), in the absence of mevalonate. The surviving cells exhibited a marked increase in a 90 kDa HMG-CoA reductase which was localized exclusively to peroxisomes. The UT2 cells grown in the absence of mevalonate containing the upregulated peroxisomal HMG-CoA reductase are designated UT2*. A detailed characterization and analysis of this cell line is presented in this study. The examination of UT2/UT2* cell cDNA and genomic DNA has led to the identification of two novel point mutations in intronic sequences of the ER HMG-CoA reductase gene. One mutation identified at the +1 position (G → A) of the 5 ′ splice site of exon 11-12 junction was shown to cause exon 11 skipping which resulted in the insertion of premature stop codons. A second mutation was also identified at the +5 position (G → A) of the 5′ splice site in the intron spanning exons 13 and 14. Furthermore, the data indicate that the two mutations in the reductase gene are present on the same allele. As demonstrated by RT-PCR of UT2 cell mRNA, the mutations produce aberrant spliced messages. If the aberrant messages were translated, truncated proteins of 44 kDa or 66 kDa would be predicted. More importantly, these truncated proteins would be expected not to have catalytic activity. Thus, the mutations identified in the ER reductase gene in UT2/UT2* cells indicate that neither a 97 kDa nor a 90 kDa reductase protein can be produced from this gene.
Book Synopsis Peroxisomal Disorders and Regulation of Genes by : Frank Roels
Download or read book Peroxisomal Disorders and Regulation of Genes written by Frank Roels and published by Springer. This book was released on 2003-12-31 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: In most peroxisomal disorders the nervous system is severely affected which explains the clinical and community burden they represent. This is the first book to focus not only on the mutations causing these inherited illnesses, but also on mechanisms that regulate, suppress or enhance expression of genes and their products (enzymes). Indeed since the success and completion of the Human Genome Project all genes (coding DNA sequences) are known. However, of many, their function, and the role of the gene product has not been determined. An example is X-linked adrenoleukodystrophy, the most frequent peroxisomal disorder. Children are born healthy, but in more than 1 out of 3, demyelination of the brain starts unpredictably and they die in a vegetative state. The gene mutated in most families has been known for 10 years; but the true role of the encoded protein, ALDp, is still speculative; and within the same family, very severe and asymptomatic clinical histories co-exist, unexplained by the mutation.
Book Synopsis The Boston Tea Party by : Allison Stark Draper
Download or read book The Boston Tea Party written by Allison Stark Draper and published by The Rosen Publishing Group, Inc. This book was released on 2001-01-15 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the series of events which led up to the Boston Tea Party and explains how this act helped bring about the war for independence.
Book Synopsis Polyunsaturated Fatty Acids in Human Nutrition by : Umberto Bracco
Download or read book Polyunsaturated Fatty Acids in Human Nutrition written by Umberto Bracco and published by Raven Press (ID). This book was released on 1992 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basado en el 28 Seminario Néstle Nutrition celebrado en Mexico, 27-30 de noviembre de 1990.
Book Synopsis Quality of Fresh and Processed Foods by : Fereidoon Shahidi
Download or read book Quality of Fresh and Processed Foods written by Fereidoon Shahidi and published by Springer Science & Business Media. This book was released on 2003-12-31 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quality is a composite term encompassing many characteristics of foods. These include color, aroma, texture, general nutrition, shelf-life, stability, and possible presence of undesirable constituents. Obviously deterioration of quality may lead to changes in the attributes that characterize the food in its fresh or freshly processed state. In addition, quality enhancement of products may be carried out using appropriate processing techniques. Interaction of different components present with one another could have a profound effect on sensory quality of products. Meanwhile, presence of extraneous matter such as pesticides and debris may also contribute to a compromise in the quality of foods. In addition, processing often brings about changes in many attributes of food including its nutritional value. Thus, examination of process-induced changes in food products is important. In this book, a cursory account of quality attributes of fresh and processed foods is provided. The book is of interest to food scientists, nutritionists and biochemists in academia, government and industry.
