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Atlas Of Human Pluripotent Stem Cells In Culture
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Book Synopsis Atlas of Human Pluripotent Stem Cells in Culture by : Lyn Healy
Download or read book Atlas of Human Pluripotent Stem Cells in Culture written by Lyn Healy and published by Springer. This book was released on 2014-11-07 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.
Book Synopsis Atlas of Human Pluripotent Stem Cells by : Michal Amit
Download or read book Atlas of Human Pluripotent Stem Cells written by Michal Amit and published by Springer Science & Business Media. This book was released on 2011-11-19 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cells.
Book Synopsis Atlas of Human Pluripotent Stem Cells by : Michal Amit
Download or read book Atlas of Human Pluripotent Stem Cells written by Michal Amit and published by Humana Press. This book was released on 2016-05-01 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive atlas offers step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions and in non-adhesion suspension culture, as well as methods for examining pluripotency and karyotype stability.
Book Synopsis Pluripotent Stem Cells by : Minoru Tomizawa
Download or read book Pluripotent Stem Cells written by Minoru Tomizawa and published by BoD – Books on Demand. This book was released on 2016-07-20 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pluripotent stem cells have distinct characteristics: self-renewal and the potential to differentiate into various somatic cells. In recent years, substantial advances have been made from basic science to clinical applications. The vast amount knowledge available makes obtaining concise yet sufficient information difficult, hence the purpose of this book. In this book, embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells are discussed. The book is divided into five sections: pluripotency, culture methods, toxicology, disease models, and regenerative medicine. The topics covered range from new concepts to current technologies. Readers are expected to gain useful information from expert contributors.
Book Synopsis Human Pluripotent Stem Cells by : Uma Lakshmipathy
Download or read book Human Pluripotent Stem Cells written by Uma Lakshmipathy and published by John Wiley & Sons. This book was released on 2018-02-13 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive coverage of the entire induced pluripotent stem cell basic work flow Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms. Includes helpful tips and tricks that are normally omitted from regular research papers Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.
Book Synopsis Induced Pluripotent Stem (iPS) Cells by : Andras Nagy
Download or read book Induced Pluripotent Stem (iPS) Cells written by Andras Nagy and published by Springer Nature. This book was released on 2022-06-22 with total page 829 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.
Book Synopsis Human Embryonic Stem Cells by : Jon Odorico
Download or read book Human Embryonic Stem Cells written by Jon Odorico and published by Garland Science. This book was released on 2004-02-01 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first successful isolation and cultivation of human embryonic stem cells at the University of Wisconsin, Madison in 1998, there has been high levels of both interest and controversy in this area of research. This book provides a concise overview of an exciting field, covering the characteristics of both human embryonic stem cells and pluripotent stem cells from other human cell lineages. The following chapters describe state-of-the-art differentiation and characterization of specific ectoderm, mesoderm and endoderm-derived lineages from human embryonic stem cells, emphasizing how these can be used to study human developmental mechanisms. A further chapter discusses genetic manipulation of human ES cells. The concluding section covers therapeutic applications of human ES cells, as well as addressing the ethical and legal issues that this research have raised.
Book Synopsis Advances in Pluripotent Stem Cells by :
Download or read book Advances in Pluripotent Stem Cells written by and published by BoD – Books on Demand. This book was released on 2024-01-31 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human pluripotent stem cells (hPSCs) are advantageous cell sources for disease remodeling and drug screening, particularly for regenerative medicine. State-of-the-art updates have highlighted the feasibility of hPSCs for the large-scale preparation of diverse kinds of stem cells and functional cells, such as mesenchymal stem/stromal cells (MSCs), hematopoietic stem cells (HSCs), neural stem cells (NSCs), natural killer (NK) cells, and chimeric antigen receptor-transduced T cells (CAR-Ts). With the aid of preclinical investigations and clinical practice, hPSCs have been recognized as promising therapeutic cell sources with excellent properties for treating a variety of refractory and recurrent diseases. This book provides a comprehensive overview of advances in pluripotent stem cells.
Book Synopsis 3D Stem Cell Culture by : Joni H. Ylostalo
Download or read book 3D Stem Cell Culture written by Joni H. Ylostalo and published by MDPI. This book was released on 2021-01-07 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.
Book Synopsis Stem Cells Heterogeneity - Novel Concepts by : Alexander Birbrair
Download or read book Stem Cells Heterogeneity - Novel Concepts written by Alexander Birbrair and published by Springer. This book was released on 2019-04-23 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive discussion on the novel concepts in stem cell heterogeneity, from pluripotent stem cells to human mesenchymal stem cells, adult and cancer stem cells of the thyroid, sarcoma, and more. Thus, Stem Cell Heterogeneity - Novel Concepts starts from a timely update on the current information on stem cells heterogeneity in various tissues and discusses new concepts and future directions. It also provides a solid foundation of the history of stem cells from specific tissues and the current applications of this knowledge in regenerative medicine. When taken as a whole, alongside its companion volumes Stem Cells Heterogeneity in Different Organs, and Stem Cells Heterogeneity in Cancer, these three books present a comprehensive reference on stem cell heterogeneity in various tissues and current and future applications for regenerative medicine. It is essential reading for advanced cell biology students as well as researchers in stem cells and clinicians.
Book Synopsis Freshney's Culture of Animal Cells by : R. Ian Freshney
Download or read book Freshney's Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2021-02-17 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.
Book Synopsis OECD Series on Testing and Assessment Guidance Document on Good In Vitro Method Practices (GIVIMP) by : OECD
Download or read book OECD Series on Testing and Assessment Guidance Document on Good In Vitro Method Practices (GIVIMP) written by OECD and published by OECD Publishing. This book was released on 2018-12-10 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past several decades, there has been a substantial increase in the availability of in vitro test methods for evaluating chemical safety in an international regulatory context. To foster confidence in in vitro alternatives to animal testing, the test methods and conditions under which ...
Book Synopsis Human Embryonic Stem Cells by : Arlene Chiu
Download or read book Human Embryonic Stem Cells written by Arlene Chiu and published by Springer Science & Business Media. This book was released on 2003-08-01 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.
Book Synopsis Cell Engineering and Regeneration by : Heinz Redl
Download or read book Cell Engineering and Regeneration written by Heinz Redl and published by Springer. This book was released on 2017-02-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference work presents the origins of cells for tissue engineering and regeneration, including primary cells, tissue-specific stem cells, pluripotent stem cells and trans-differentiated or reprogrammed cells. There is particular emphasis on current understanding of tissue regeneration based on embryology and evolution studies, including mechanisms of amphibian regeneration. The book covers the use of autologous versus allogeneic cell sources, as well as various procedures used for cell isolation and cell pre-conditioning , such as cell sorting, biochemical and biophysical pre-conditioning, transfection and aggregation. It also presents cell modulation using growth factors, molecular factors, epigenetic approaches, changes in biophysical environment, cellular co-culture and other elements of the cellular microenvironment. The pathways of cell delivery are discussed with respect to specific clinical situations, including delivery of ex vivo manipulated cells via local and systemic routes, as well as activation and migration of endogenous reservoirs of reparative cells. The volume concludes with an in-depth discussion of the tracking of cells in vivo and their various regenerative activities inside the body, including differentiation, new tissue formation and actions on other cells by direct cell-to-cell communication and by secretion of biomolecules.
Book Synopsis Lung Stem Cells in Development, Health and Disease by : Nikolic, Marko Z.
Download or read book Lung Stem Cells in Development, Health and Disease written by Nikolic, Marko Z. and published by European Respiratory Society. This book was released on 2021-04-01 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most organs in the adult human body are able to maintain themselves and undergo repair after injury; these processes are largely dependent on stem cells. In this Monograph, the Guest Editors bring together leading authors in the field to provide information about the different classes of stem cells present both in the developing and adult lung: where they are found, how they function in homeostasis and pathologic conditions, the mechanisms that regulate their behaviour, and how they may be harnessed for therapeutic purposes. The book focuses on stem cells in the mouse and human lung but also includes the ferret as an increasingly important new model organism. Chapters also discuss how lung tissue, including endogenous stem cells, can be generated in vitro from pluripotent stem cell lines. This state-of-the-art collection comprehensively covers one of the most exciting areas of respiratory science
Download or read book Stem Cells written by Ariff Bongso and published by World Scientific. This book was released on 2011 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell biology has drawn tremendous interest in recent years as it promises cures for a variety of incurable diseases. This book deals with the basic and clinical aspects of stem cell research and involves work on the full spectrum of stem cells isolated today. It also covers the conversion of stem cell types into a variety of useful tissues which may be used in the future for transplantation therapy. It is thus aimed at undergraduates, postgraduates, scientists, embryologists, doctors, tissue engineers and anyone who wishes to gain some insight into stem cell biology. This book is important as it is comprehensive and covers all aspects of stem cell biology, from basic research to clinical applications. It will have 33 chapters written by renowned stem cell scientists worldwide. It will be up-to-date and all the chapters include self-explanatory figures, color photographs, graphics and tables. It will be easy to read and give the reader a complete understanding and state of the art of the exciting science and its applications.
Book Synopsis Atlas of Interventional Orthopedics Procedures by : Christopher J. Williams
Download or read book Atlas of Interventional Orthopedics Procedures written by Christopher J. Williams and published by Elsevier Health Sciences. This book was released on 2022-02-25 with total page 659 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of interventional orthopedics is changing the landscape of orthopedic care as patients seek less invasive options for the treatment of common conditions like arthritis, rotator cuff tears, and degenerative disc disease. Offering easy-to-follow, step-by-step guidance on both peripheral joint and spinal procedures, Atlas of Interventional Orthopedics Procedures is the first reference to provide this practical content in one authoritative, user-friendly text. Abundantly illustrated and easy to read, it presents simple to advanced injection skills covering all orthopedic and physical medicine procedures using up-to-date imaging techniques. Presents foundational knowledge for interventional orthopedics as well as ultrasound and x-ray guided techniques for both peripheral joint and spinal procedures. Features nearly 1,000 high-quality images including fluoroscopy, MRIs, procedural images, and unique anatomical illustrations drawn by a physical medicine and rehabilitation physician. Covers need-to-know topics such as autologous orthobiologics, allogenic tissue grafts, prolotherapy, and principles of fluoroscopy and ultrasound injection techniques. Offers several ultrasound and fluoroscopy images for each procedure, as well as step-by-step descriptions and the authors’ preferred technique. Walks you through general injection techniques such as interventional spine procedures, peripheral joint injections, and spinal and peripheral ligament, tendon, and nerve techniques; advanced techniques include intraosseous injections, needle arthroscopy, perineural hydrodissection, and emerging interventional techniques. Provides an up-to-date review on regenerative medicine for musculoskeletal pathology from editors and authors who are leading physicians in the field. Follows the core tenets of interventional orthopedics, including injectates that can facilitate healing of musculoskeletal tissues, precise placement of those injectates into damaged structures using imaging guidance, and the eventual development of new tools to facilitate percutaneous tissue manipulation.
