Human Stem Cell Manual

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Human Stem Cell Manual

Author : Suzanne Peterson
Publisher : Academic Press
ISBN 13 : 0123854741
Total Pages : 656 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Human Stem Cell Manual by : Suzanne Peterson

Download or read book Human Stem Cell Manual written by Suzanne Peterson and published by Academic Press. This book was released on 2012-10-22 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

Human Stem Cell Technology and Biology

Author : Gary S. Stein
Publisher : John Wiley & Sons
ISBN 13 : 0470889896
Total Pages : 439 pages
Book Rating : 4.4/5 (78 download)

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Book Synopsis Human Stem Cell Technology and Biology by : Gary S. Stein

Download or read book Human Stem Cell Technology and Biology written by Gary S. Stein and published by John Wiley & Sons. This book was released on 2011-03-04 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Culture of Human Stem Cells

Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 9780470167519
Total Pages : 360 pages
Book Rating : 4.1/5 (675 download)

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Book Synopsis Culture of Human Stem Cells by : R. Ian Freshney

Download or read book Culture of Human Stem Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2007-07-16 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Human Embryonic Stem Cells

Author : Stephen Sullivan
Publisher : John Wiley & Sons
ISBN 13 : 9780470511596
Total Pages : 424 pages
Book Rating : 4.5/5 (115 download)

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Book Synopsis Human Embryonic Stem Cells by : Stephen Sullivan

Download or read book Human Embryonic Stem Cells written by Stephen Sullivan and published by John Wiley & Sons. This book was released on 2007-06-13 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in purusing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research. The novel and useful focus of this book sets it apart from other available books: Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells Centred solely on practical protocols for human (not mouse) embryonic stem cell research Includes extensive troubleshooting sections Addresses the different proclivities and behaviours of individual human embryonic cell lines Contains techniques currently known only to a small number of specialised laboratories worldwide This handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases. Praise from the reviews: "...a highly readable and useful book... A notable feature of the book is its air of openness and honesty... This book... will help many to navigate the uncharted waters of human embryonic stem cell biology." BRITISH SOCIETY FOR CELL BIOLOGY "... the imaginative solutions in this book can inspire us to get past our most frustrating limitations." CELL STEM CELL "... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE "In this fast-moving field, this [handbook] will help drive advances of more and more researchers." DIFFERENTIATION "...a valuable resource for seasoned and novice researchers... an excellent addition to the reference collection of any medical library or research laboratory." THE AMERICAN MEDICAL ASSOCIATION

Stem Cells Handbook

Author : Stewart Sell
Publisher : Springer Science & Business Media
ISBN 13 : 1461476968
Total Pages : 534 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Stem Cells Handbook by : Stewart Sell

Download or read book Stem Cells Handbook written by Stewart Sell and published by Springer Science & Business Media. This book was released on 2013-08-16 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses critical areas of progress in stem cell research, including the most recent research and applications of pluripotent embryonic cells, induced pluripotent cells, oligopotent tissue stem cells and cancer stem cells. The text covers basic knowledge of stem cell biology, stem cell ethics, development of techniques for applying stem cell therapy, the technology of obtaining appropriate cells for transplantation as well as the role of stem cells in cancer and how therapy may be directed to cancer stem cells. This new volume is essential reading for all scientists currently in the field or allied research areas, and those for those graduate students who envision a career in stem cells.

Handbook of Stem Cells

Author : Anthony Atala
Publisher : Academic Press
ISBN 13 : 0123859433
Total Pages : 1076 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Handbook of Stem Cells by : Anthony Atala

Download or read book Handbook of Stem Cells written by Anthony Atala and published by Academic Press. This book was released on 2012-12-31 with total page 1076 pages. Available in PDF, EPUB and Kindle. Book excerpt: New discoveries in the field of stem cells increasingly dominate the news and scientific literature revealing an avalanche of new knowledge and research tools that are producing therapies for cancer, heart disease, diabetes, and a wide variety of other diseases that afflict humanity. The Handbook of Stem Cells integrates this exciting area of life science, combining in two volumes the requisites for a general understanding of adult and embryonic stem cells. Organized in two volumes entitled Pluripotent Stem Cells and Cell Biology and Adult and Fetal Stem Cells, this work contains contributions from the world’s experts in stem cell research to provide a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations as well as a the latest information of what is known about each specific organ system. Provides comprehensive coverage on this highly topical subject Contains contributions by the foremost authorities and premiere names in the field of stem cell research Companion website - http://booksite.elsevier.com/9780123859426/ - contains over 250 color figures in presentation format

Stem Cell Manufacturing

Author : Joaquim M. S. Cabral
Publisher : Elsevier
ISBN 13 : 0444632662
Total Pages : 340 pages
Book Rating : 4.4/5 (446 download)

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Book Synopsis Stem Cell Manufacturing by : Joaquim M. S. Cabral

Download or read book Stem Cell Manufacturing written by Joaquim M. S. Cabral and published by Elsevier. This book was released on 2016-07-24 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem Cell Manufacturing discusses the required technologies that enable the transfer of the current laboratory-based practice of stem cell tissue culture to the clinic environment as therapeutics, while concurrently achieving control, reproducibility, automation, validation, and safety of the process and the product. The advent of stem cell research unveiled the therapeutic potential of stem cells and their derivatives and increased the awareness of the public and scientific community for the topic. The successful manufacturing of stem cells and their derivatives is expected to have a positive impact in the society since it will contribute to widen the offer of therapeutic solutions to the patients. Fully defined cellular products can be used to restore the structure and function of damaged tissues and organs and to develop stem cell-based cellular therapies for the treatment of cancer and hematological disorders, autoimmune and other inflammatory diseases and genetic disorders. Presents the first ‘Flowchart‘ of stem cell manufacturing enabling easy understanding of the various processes in a sequential and coherent manner Covers all bioprocess technologies required for the transfer of the bench findings to the clinic including the process components: cell signals, bioreactors, modeling, automation, safety, etc. Presents comprehensive coverage of a true multidisciplinary topic by bringing together specialists in their particular area Provides the basics of the processes and identifies the issues to be resolved for large scale cell culture by the bioengineer Addresses the critical need in bioprocessing for the successful delivery of stem cell technology to the market place by involving professional engineers in sections of the book

Human Pluripotent Stem Cells

Author : Uma Lakshmipathy
Publisher : John Wiley & Sons
ISBN 13 : 1119394333
Total Pages : 175 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis Human Pluripotent Stem Cells by : Uma Lakshmipathy

Download or read book Human Pluripotent Stem Cells written by Uma Lakshmipathy and published by John Wiley & Sons. This book was released on 2018-02-13 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive coverage of the entire induced pluripotent stem cell basic work flow Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms. Includes helpful tips and tricks that are normally omitted from regular research papers Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

Manual of Stem Cell and Bone Marrow Transplantation

Author : Joseph H. Antin
Publisher : Cambridge University Press
ISBN 13 : 1107661544
Total Pages : 204 pages
Book Rating : 4.1/5 (76 download)

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Book Synopsis Manual of Stem Cell and Bone Marrow Transplantation by : Joseph H. Antin

Download or read book Manual of Stem Cell and Bone Marrow Transplantation written by Joseph H. Antin and published by Cambridge University Press. This book was released on 2013-10-17 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fully revised second edition is a practical manual for all members of the stem cell and bone marrow transplant team.

Stem Cells: An Insider's Guide

Author : Paul Knoepfler
Publisher : World Scientific
ISBN 13 : 9814508829
Total Pages : 368 pages
Book Rating : 4.8/5 (145 download)

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Book Synopsis Stem Cells: An Insider's Guide by : Paul Knoepfler

Download or read book Stem Cells: An Insider's Guide written by Paul Knoepfler and published by World Scientific. This book was released on 2013-07-30 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem Cells: An Insider's Guide is an exciting new book that takes readers inside the world of stem cells guided by international stem cell expert, Dr. Paul Knoepfler. Stem cells are catalyzing a revolution in medicine. The book also tackles the exciting and hotly debated area of stem cell treatments that are capturing the public's imagination. In the future they may also transform how we age and reproduce. However, there are serious risks and ethical challenges, too. The author's goal with this insider's guide is to give readers the information needed to distinguish between the ubiquitous hype and legitimate hope found throughout the stem cell world. The book answers the most common questions that people have about stem cells. Can stem cells help my family with a serious medical problem such as Alzheimer's, Multiple Sclerosis, or Autism? Are such treatments safe? Can stem cells make me look younger or even literally stay physically young? These questions and many more are answered here.A number of ethical issues related to stem cells that spark debates are discussed, including risky treatments, cloning and embryonic stem cells. The author breaks new ground in a number of ways such as by suggesting reforms to the FDA, providing a new theory of aging based on stem cells, and including a revolutionary Stem Cell Patient Bill of Rights. More generally, the book is your guide to where the stem cell field will be in the near future as well as a thoughtful perspective on how stem cell therapies will ultimately change your life and our world.

Manual For Primary Human Cell Culture, A (2nd Edition)

Author : Schantz Jan-thorsten
Publisher : World Scientific Publishing Company
ISBN 13 : 9813101210
Total Pages : 212 pages
Book Rating : 4.8/5 (131 download)

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Book Synopsis Manual For Primary Human Cell Culture, A (2nd Edition) by : Schantz Jan-thorsten

Download or read book Manual For Primary Human Cell Culture, A (2nd Edition) written by Schantz Jan-thorsten and published by World Scientific Publishing Company. This book was released on 2010-01-04 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.

Handbook of Stem Cells, Two-Volume Set

Author : Robert Lanza
Publisher : Elsevier
ISBN 13 : 9780080533735
Total Pages : 1760 pages
Book Rating : 4.5/5 (337 download)

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Book Synopsis Handbook of Stem Cells, Two-Volume Set by : Robert Lanza

Download or read book Handbook of Stem Cells, Two-Volume Set written by Robert Lanza and published by Elsevier. This book was released on 2004-10-05 with total page 1760 pages. Available in PDF, EPUB and Kindle. Book excerpt: New discoveries in the field of stem cell research have frequently appeared in the news and in scientific literature. Research in this area promises to lead to new therapies for cancer, heart disease, diabetes, and a wide variety of other diseases. This two-volume reference integrates this exciting area of biology, combining the prerequisites for a general understanding of adult and embryonic stem cells, the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations, as well as a presentation by the world's experts of what is currently known about each specific organ system. The editors of the Handbook of Stem Cells include: Robert Lanza, Helen Blau, John Gearhart, Brigid Hogan, Douglas Melton, Malcolm Moore, Roger Pedersen, E. Donnall Thomas, James Thomson, Catherine Verfaillie, Irving Weissman, and Michael West. The Editorial Board includes: W. French Anderson, Peter Andrews, Anthony Atala, Jose Cibelli, Giulio Cossu, Robert Edwards, Martin Evans, Elaine Fuchs, Margaret Fuller, Fred Gage, Richard Gardner, Margaret Goodell, Ronald Green, William Haseltine, Joseph Itskovitz-Eldor, Rudolf Jaenisch, Ihor Lemischka, Dame Anne McLaren, Richard Mulligan, Stuart Orkin, Martin Pera, Benjamin Reubinoff, Janet Rossant, Hans Scholer, Austin Smith, Evan Snyder, Davor Solter, Alan Trounson, and Leonard Zon. This comprehensive set should be a much-needed addition to the library of students and researchers alike. * Provides comprehensive coverage on this highly topical subject * Contains contributions by the foremost authorities and premiere names in the field of stem cell research * The accompanying CD-ROM includes over 250 color figures

Preservation of Cells

Author : Allison Hubel
Publisher : John Wiley & Sons
ISBN 13 : 1118989848
Total Pages : 212 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Preservation of Cells by : Allison Hubel

Download or read book Preservation of Cells written by Allison Hubel and published by John Wiley & Sons. This book was released on 2018-02-13 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Helps those that use cell preservation to develop new protocols or improve existing protocols This book provides readers with the tools needed to develop or debug a preservation protocol for cells. The core structure and content of the text grew from a professional short course that has been offered at the Biopreservation Core Resource for the last 10 years. This comprehensive text describes, step by step, the individual elements of a protocol, including the relevant scientific principles for each phase of the protocol. It can be used by anyone who is involved in cell preservation—even by those who are not experts in freezing of cells—because it provides the scientific basis for those that want to understand the basis for the protocol. Preservation of Cells: A Practical Manual begins by first introducing readers to the subject of preserving cells. It then goes on to cover Pre-freeze Processing and Characterization; Formulation and Introduction of Cryopreservation Solutions; Freezing Protocols; Storage and Shipping of Frozen Cells; Thawing and Post Thaw Processing; Post-thaw Assessment; and Algorithm-driven Protocol Optimization. Clearly explains the reasons behind every step in the development of a preservation protocol and the scientific principles behind them Provides alternative modes of preservation for when conventional methods of cryopreservation are not appropriate for a given cell type or application Enables more organization to achieve improved post thaw recoveries and process consistency Preservation of Cells: A Practical Manual is an important book for researchers, laboratory technicians and students in cell biology, stem cell biology, tissue engineering, and regenerative medicine. It is also useful to cell bankers, regenerative medicine, biomarker discovery or precision medicine companies, and cell therapy labs, blood bankers, biobankers, and biotechnology companies.

Human Embryonic Stem Cells

Author : Arlene Chiu
Publisher : Springer Science & Business Media
ISBN 13 : 1592594239
Total Pages : 455 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Human Embryonic Stem Cells by : Arlene Chiu

Download or read book Human Embryonic Stem Cells written by Arlene Chiu and published by Springer Science & Business Media. This book was released on 2003-08-01 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.

The EBMT Handbook

Author : Nicolaus Kröger
Publisher :
ISBN 13 : 9781013273674
Total Pages : 688 pages
Book Rating : 4.2/5 (736 download)

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Book Synopsis The EBMT Handbook by : Nicolaus Kröger

Download or read book The EBMT Handbook written by Nicolaus Kröger and published by . This book was released on 2020-10-08 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Open Access edition of the European Society for Blood and Marrow Transplantation (EBMT) handbook addresses the latest developments and innovations in hematopoietic stem cell transplantation and cellular therapy. Consisting of 93 chapters, it has been written by 175 leading experts in the field. Discussing all types of stem cell and bone marrow transplantation, including haplo-identical stem cell and cord blood transplantation, it also covers the indications for transplantation, the management of early and late complications as well as the new and rapidly evolving field of cellular therapies. This book provides an unparalleled description of current practices to enhance readers' knowledge and practice skills. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.

Stem Cells

Author : Ariff Bongso
Publisher : World Scientific
ISBN 13 : 9814289388
Total Pages : 721 pages
Book Rating : 4.8/5 (142 download)

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Book Synopsis Stem Cells by : Ariff Bongso

Download or read book Stem Cells written by Ariff Bongso and published by World Scientific. This book was released on 2011 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell biology has drawn tremendous interest in recent years as it promises cures for a variety of incurable diseases. This book deals with the basic and clinical aspects of stem cell research and involves work on the full spectrum of stem cells isolated today. It also covers the conversion of stem cell types into a variety of useful tissues which may be used in the future for transplantation therapy. It is thus aimed at undergraduates, postgraduates, scientists, embryologists, doctors, tissue engineers and anyone who wishes to gain some insight into stem cell biology. This book is important as it is comprehensive and covers all aspects of stem cell biology, from basic research to clinical applications. It will have 33 chapters written by renowned stem cell scientists worldwide. It will be up-to-date and all the chapters include self-explanatory figures, color photographs, graphics and tables. It will be easy to read and give the reader a complete understanding and state of the art of the exciting science and its applications.

Handbook of Stem Cell Therapy

Author : Khawaja Husnain Haider
Publisher : Springer Nature
ISBN 13 : 9811926557
Total Pages : 1527 pages
Book Rating : 4.8/5 (119 download)

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Book Synopsis Handbook of Stem Cell Therapy by : Khawaja Husnain Haider

Download or read book Handbook of Stem Cell Therapy written by Khawaja Husnain Haider and published by Springer Nature. This book was released on 2022-11-07 with total page 1527 pages. Available in PDF, EPUB and Kindle. Book excerpt: The handbook comprehensively reviews the therapeutic potential of stem cells and stem cell secretome-based cell-free strategies in regenerative medicine. The chapters in section I and section II respectively discuss the diverse applications of mesenchymal stem cells and non- mesenchymal stem cells, including skeletal myoblasts, endothelial progenitor cells, adipose tissue-derived stem cells, induced pluripotent stem cells, and neuronal stem cells in myocardial repair, inflammatory bowel disease, cognitive deficits, wound healing, retinal disorders, and COVID-19. The subsequent chapters in section IIIprimarily focused on the fast-emerging cell-free therapy approach in regenerative medicine for tissue repair and regeneration. These chapters review the impact of stem cell-derived secretome on various biological processes such as angiogenesis, neurogenesis, tissue repair, immunomodulation, musculoskeletal pathologies, wound healing, anti-fibrotic, and anti-tumorigenesis for tissue maintenance and regeneration.Lastly, section IV summarizes miscellaneous aspects of cell-based therapy, includingthe treatment advantages, opportunities, and shortcomings in stem cell-based therapy, potentially helping to refine future studies and translate them from experimental to clinical studies. Moreover, this section also has chapters on cancer stem cells as novel targets in cancer therapeutics. This Major Reference Book (MRW) is a valuable resource for researchers involved in stem cell research to understand the multifaceted therapeutic applications of stem cells and their derivative secretome in regenerative medicine.