Ultrafast Excited-state Dynamics of Green Fluorescent Proteins and Fluorescein-antibody Complexes

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ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Ultrafast Excited-state Dynamics of Green Fluorescent Proteins and Fluorescein-antibody Complexes by : Xinghua Shi

Download or read book Ultrafast Excited-state Dynamics of Green Fluorescent Proteins and Fluorescein-antibody Complexes written by Xinghua Shi and published by . This book was released on 2007 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Ultrafast Excited-state Dynamics of Fluorescent Proteins

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Publisher :
ISBN 13 :
Total Pages : 344 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Ultrafast Excited-state Dynamics of Fluorescent Proteins by : Paul Abbyad

Download or read book Ultrafast Excited-state Dynamics of Fluorescent Proteins written by Paul Abbyad and published by . This book was released on 2007 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Ultrafast Dynamics in the Photosynthetic Reaction Center and Green Fluorescent Proteins

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ISBN 13 :
Total Pages : 514 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Ultrafast Dynamics in the Photosynthetic Reaction Center and Green Fluorescent Proteins by : Brett Andrew King

Download or read book Ultrafast Dynamics in the Photosynthetic Reaction Center and Green Fluorescent Proteins written by Brett Andrew King and published by . This book was released on 2000 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fluorescent Proteins I

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Publisher : Springer Science & Business Media
ISBN 13 : 3642233724
Total Pages : 275 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis Fluorescent Proteins I by : Gregor Jung

Download or read book Fluorescent Proteins I written by Gregor Jung and published by Springer Science & Business Media. This book was released on 2011-10-25 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins I is devoted to the basic photophysical and photochemical aspects of fluorescent protein technology. Experienced experts highlight colour tuning, the exploration of switching phenomena and respective methods for their investigation. The book provides a thorough understanding of primary molecular processes allowing the design of fluorescent proteins for specific applications.

Green Fluorescent Proteins

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Publisher : Gulf Professional Publishing
ISBN 13 : 9780126760750
Total Pages : 430 pages
Book Rating : 4.7/5 (67 download)

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Book Synopsis Green Fluorescent Proteins by : American Society for Cell Biology

Download or read book Green Fluorescent Proteins written by American Society for Cell Biology and published by Gulf Professional Publishing. This book was released on 1999 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features * Only volume on Green Fluorescent Protein research * Covers all aspects of GFP * Provides specific microscopic and data analysis methods * Discusses the design and construction of GFP fusion proteins * Covers GFP expression in animals, insects, plants, and microbes * Details procedures for time lapse imaging of living cells * Explains how to implement single molecule fluorescence detection with GFP * Discusses dual label GFP strategies for multicolor fluorescence * Presents fluorescence resonance energy transfer methods with GFPs * Details quantitative fluorescence imaging techniques * Extensively illustrated with color photographs

Green Fluorescent Protein

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Publisher : John Wiley & Sons
ISBN 13 : 0471739480
Total Pages : 486 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Green Fluorescent Protein by : Martin Chalfie

Download or read book Green Fluorescent Protein written by Martin Chalfie and published by John Wiley & Sons. This book was released on 2005-11-18 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Excited State Dynamics in Fluorescent Proteins (GFP, RFP) and Flavoproteins

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Publisher :
ISBN 13 :
Total Pages : 156 pages
Book Rating : 4.:/5 (18 download)

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Book Synopsis Excited State Dynamics in Fluorescent Proteins (GFP, RFP) and Flavoproteins by : Tanja Angela Schüttrigkeit

Download or read book Excited State Dynamics in Fluorescent Proteins (GFP, RFP) and Flavoproteins written by Tanja Angela Schüttrigkeit and published by . This book was released on 2004 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Ultrafast Fluorescence Studies of Excited-state Dynamics of a Few Organic Chromophores in Solution

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Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (663 download)

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Book Synopsis Ultrafast Fluorescence Studies of Excited-state Dynamics of a Few Organic Chromophores in Solution by : Emile van Veldhoven

Download or read book Ultrafast Fluorescence Studies of Excited-state Dynamics of a Few Organic Chromophores in Solution written by Emile van Veldhoven and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Excited State Dynamics in Fluorescent Proteins (GFP, RFP) and Flavoproteins

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ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (696 download)

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Book Synopsis Excited State Dynamics in Fluorescent Proteins (GFP, RFP) and Flavoproteins by :

Download or read book Excited State Dynamics in Fluorescent Proteins (GFP, RFP) and Flavoproteins written by and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Deactivation channels of excited states in green and red fluorescing proteins (GFP, RFP) and mutants as well as flavoproteins have been investigated by steady-state and femtosecond/picosecond time-resolved fluorescence/absorption spectroscopy. Besides fast internal conversion, energy transfer between chromophores in different maturation states in oligomers of DsRed as well as from the antenna molecule to the flavin cofactor in (6-4) photolyase and cryptochrom2 has been established as photophysical primary process. In cryptochrom1, electron transfer from nearby tyrosyl and tryptophane residues to FAD is the initial step in signal transduction, as is intersystem crossing in the LOV2 domain in phototropin.

Fluorescent Proteins

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Publisher : Elsevier
ISBN 13 : 0080557244
Total Pages : 613 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Fluorescent Proteins by : Kevin F. Sullivan

Download or read book Fluorescent Proteins written by Kevin F. Sullivan and published by Elsevier. This book was released on 2007-12-14 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.

Photophysics of Emission Color in Flourescent Proteins

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ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.:/5 (192 download)

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Book Synopsis Photophysics of Emission Color in Flourescent Proteins by : Xiaokun Shu

Download or read book Photophysics of Emission Color in Flourescent Proteins written by Xiaokun Shu and published by . This book was released on 2007 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Ultrafast Excited State Dynamics of Inorganic Molecules Related to Modern Light Harvesting Applications

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ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (137 download)

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Book Synopsis Ultrafast Excited State Dynamics of Inorganic Molecules Related to Modern Light Harvesting Applications by : Firew Tarekegn Gemeda

Download or read book Ultrafast Excited State Dynamics of Inorganic Molecules Related to Modern Light Harvesting Applications written by Firew Tarekegn Gemeda and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Halide complexes of heavy metals attract great attention due to their applications in photonics and light harvesting (collection of solar energy through photochemical processes with subsequent conversion into chemical energy). Ultrafast spectroscopic studies deliver the molecular level understanding of very initial physicochemical processes, which often define the useful functions these complexes provide, and can be used for rationally designing the materials in which these complexes are the building blocks. We aim to investigate ultrafast excited-state reaction dynamics and photophysical/photochemical relaxation mechanisms of hexahalide complexes of heavy metals. In the first part of this work, the excited-state relaxation pathways in a Bi(III) hexahalide, BiX63-, (X = I, Br) a paradigm complex for understanding the ns2 main group metals, are studied by means of femtosecond transient absorption spectroscopy supported by nanosecond transient absorption spectroscopy, steady-state absorption/emission measurements and DFT computations. Radiationless relaxation out of the initially excited, predominantly metal-centered (MC) triply degenerate 3T1u state, populates two lower-energy states: a ligand-to-metal-charge transfer (LMCT) excited state of the 3Ï0 LMCT I(npÏ0)2̐ʼBi(6p) nature and a luminescent 'trap' 3A1u(3P0) MC state. On a nanosecond timescale, the 3Ï0 LMCT population decays through other intermediates to generate the triplet species (acetonitrile solvent), which is identified as the Îʺ2 metal ligated dihalide-bismuth adduct with the intramolecularly formed X-X bond, [(Îʺ2-X2)Bi(II)X4]38́2, the product of interest for applications in solar energy conversion and storage. In the second part of this work, the ultrafast dynamics of IrCl62-, a prototype low-spin d5 metal complex, excited into intraconfigurational short-wavelength-infrared MC and visible LMCT electronic states is studied by femtosecond transient absorption spectroscopy. Upon any excitation of the complex in water or acetonitrile solutions, the excited-state molecules are found to relax into the ground electronic state via a cascade-like relaxation mechanism. Excited-state Jahn-Teller effect is a driving force for the relaxation dynamics even in this case where substantial spin-orbit interactions in the molecular system are present. Femtosecond pulsed state-selective excitation undertaken in this project is shown to be essential for unravelling the complex excited-state relaxation mechanism of the heavy metal complexes, providing new understanding of their structure and function.

Fluorescence Spectroscopy

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Publisher : Academic Press
ISBN 13 : 0080923348
Total Pages : 403 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Fluorescence Spectroscopy by :

Download or read book Fluorescence Spectroscopy written by and published by Academic Press. This book was released on 2011-09-06 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics. Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique Provides tried and tested techniques which eliminate searching through many different sources

Ultrafast Excited-state Dynamics in Multichromophoric Systems

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ISBN 13 :
Total Pages : 212 pages
Book Rating : 4.:/5 (14 download)

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Book Synopsis Ultrafast Excited-state Dynamics in Multichromophoric Systems by : Diego Villamaina

Download or read book Ultrafast Excited-state Dynamics in Multichromophoric Systems written by Diego Villamaina and published by . This book was released on 2014 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fluorescent Analogs of Biomolecular Building Blocks

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Publisher : John Wiley & Sons
ISBN 13 : 1118175867
Total Pages : 474 pages
Book Rating : 4.1/5 (181 download)

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Book Synopsis Fluorescent Analogs of Biomolecular Building Blocks by : Marcus Wilhelmsson

Download or read book Fluorescent Analogs of Biomolecular Building Blocks written by Marcus Wilhelmsson and published by John Wiley & Sons. This book was released on 2016-04-04 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.

Book of Abstracts

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Publisher :
ISBN 13 :
Total Pages : 776 pages
Book Rating : 4.0/5 ( download)

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Book Synopsis Book of Abstracts by :

Download or read book Book of Abstracts written by and published by . This book was released on 2000 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Principles of Fluorescence Spectroscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 146157658X
Total Pages : 487 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.