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Transcriptional And Post Transcriptional Regulation Of The Cit1 Gene In Saccharomyces Cerevisiae
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Book Synopsis Transcriptional and Post-transcriptional Regulation of the CIT1 Gene in Saccharomyces Cerevisiae by : Sobomabo D. Lawson
Download or read book Transcriptional and Post-transcriptional Regulation of the CIT1 Gene in Saccharomyces Cerevisiae written by Sobomabo D. Lawson and published by . This book was released on 1995 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Post-transcriptional Regulation of Gene Expression by the (PSI) Prion of Saccharomyces Cerevisiae by : Jonathan Paul Richardson
Download or read book Post-transcriptional Regulation of Gene Expression by the (PSI) Prion of Saccharomyces Cerevisiae written by Jonathan Paul Richardson and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mechanisms of Post-Transcriptional Regulation in S. Cerevisiae by : Vince Harjono
Download or read book Mechanisms of Post-Transcriptional Regulation in S. Cerevisiae written by Vince Harjono and published by . This book was released on 2022 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt: Post-transcriptional regulation represents a powerful and versatile mechanism to fine-tune gene expression to meet cellular and environmental demands. One important aspect of post-transcriptional regulation involves regulation of protein translation, the process of building proteins from a messenger RNA. In this dissertation, I use biochemical and molecular biology techniques to study how translation is mechanistically regulated by both mRNA and protein factors. In chapter 2, I discuss the development of a quantitative method in eukaryotes to measure ribosomal stalls of cis-mRNA factors on protein elongation. We find that different distributions of nonoptimal codons trigger different surveillance and rescue pathways despite similar levels of elongation delay. In chapter 3, I explore the relationship between translatability and mRNA localization during glucose starvation and investigate potential factors that influence this relationship. We find that a complex made from Rvb1 and Rvb2 is involved in promoter-directed cytoplasmic fate in a subset of stress response genes in glucose starvation. Furthermore, we use carefully designed reporters to interrogate how translatability determines cytoplasmic localization and find that active translation is linked to exclusion from stress-induced cytoplasmic granules. Finally in chapter 4, I discuss improvements on the method we have developed, possible future directions for the work described in this dissertation, and my concluding remarks.
Book Synopsis Proteomic and Transcriptomic Analyses Reveal Novel Aspects of Post-transcriptional Regulation in Saccharomyces Cerevisiae by : Gregory A. Cary
Download or read book Proteomic and Transcriptomic Analyses Reveal Novel Aspects of Post-transcriptional Regulation in Saccharomyces Cerevisiae written by Gregory A. Cary and published by . This book was released on 2013 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although gene expression begins with transcription, there are a variety of mechanisms that cells use to control and tune expression post-transcriptionally. Many post-transcriptional regulatory functions including translational regulation, transcript surveillance, intracellular RNA localization, and RNA decay occur in organelles known as RNA granules. RNA granules, such as processing (P)-bodies, are cytoplasmic accumulations of translationally repressed mRNA and associated proteins that are ubiquitous among eukaryotes. Much of what is known about RNA granule biology has been observed through genetic and cytological experimentation and very few biochemical enrichments of these structures have been reported. In this work I present an affinity enrichment strategy for Dhh1, a conserved core component of P-bodies, from the budding yeast Saccharomyces cerevisiae. We identify proteins co-enriching with Dhh1 using tandem mass spectrometry and show that many known RNA granule proteins are enriched by this approach. We go on to compare the association of proteins with the complex across two environmental conditions to examine the effect of stress induction on RNA granule assemblies. We find that metabolic enzymes and molecular chaperones are typically more abundant in the stress-induced P-body complex and demonstrate that one chaperone, YDJ1, is involved in the stress-induced aggregation of several P-body proteins into cytoplasmic foci. We also identify RNA co-enriching with Dhh1 and detect several classes of catalytic RNA as well as a strong enrichment for the mRNA encoding the P-body protein PAT1. Finally, I present and discuss the characterization of a yeast strain that exhibits sensitivity to the drug puromycin. The puromycin-sensitive strain incorporates the drug into nascent proteins in vivo and I discuss how this is a unique and useful approach for the detection of protein biosynthesis. The techniques developed and employed in this dissertation provide novel perspectives on post-transcriptional regulatory processes and enable further investigations into how these regulatory programs are executed within the cell.
Book Synopsis Transcriptional and Post-transcriptional Regulation of the General Amino Acid Permease of Saccharomyces Cerevisiae by : Michael Stanbrough
Download or read book Transcriptional and Post-transcriptional Regulation of the General Amino Acid Permease of Saccharomyces Cerevisiae written by Michael Stanbrough and published by . This book was released on 1993 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Genome-wide Analysis of Transcriptional Expression Programs, Regulatory Networks and Cis-regulatory Sequences in Saccharomyces Cerevisiae by : Christopher T. Harbison
Download or read book Genome-wide Analysis of Transcriptional Expression Programs, Regulatory Networks and Cis-regulatory Sequences in Saccharomyces Cerevisiae written by Christopher T. Harbison and published by . This book was released on 2005 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Historically, knowledge of gene-specific transcription has been accumulated by the study of the individual genetic and physical interactions between transcriptional regulators and the genes they regulate, often requiring considerable time and effort. Microarray technology now enables investigation of gene expression at the level of the entire genome, allowing researchers access to rich datasets and promising new levels of depth in the understanding of transcriptional regulation. Our lab has made use of these technologies both to measure the levels of all mRNA transcripts within a population of cells, as well as to locate the regions within the genome that are bound by transcriptional regulators. Such studies not only allow for the functional annotation of both genes and regulators, but can also provide clues about the identity of the regulatory regions within DNA, the structure of global regulatory networks and the regulation of DNA-binding proteins. These and other insights are presented here based on our genome-wide studies of transcriptional regulation in the yeast Saccharomyces cerevisiae.
Book Synopsis Transcriptional Regulation of a Stress Inducible Gene of Saccharomyces Cerevisiae by : Naoko Kobayashi
Download or read book Transcriptional Regulation of a Stress Inducible Gene of Saccharomyces Cerevisiae written by Naoko Kobayashi and published by . This book was released on 1990 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Transcriptional Regulation of Oxygen-responsive Genes in Saccharomyces Cerevisiae by : Johanna Josephina Maria ter Linde
Download or read book Transcriptional Regulation of Oxygen-responsive Genes in Saccharomyces Cerevisiae written by Johanna Josephina Maria ter Linde and published by . This book was released on 2003 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Coordinate Regulation of Glycolytic Gene Expression in Saccharomyces Cerevisiae by : Chang Seo Park
Download or read book Coordinate Regulation of Glycolytic Gene Expression in Saccharomyces Cerevisiae written by Chang Seo Park and published by . This book was released on 1994 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Transcriptional Regulation of the Saccharomyces Cerevisiae Gene Encoding the Small Subunit of Ribonucleotide Reductase by : Holly Kae Hurd
Download or read book Transcriptional Regulation of the Saccharomyces Cerevisiae Gene Encoding the Small Subunit of Ribonucleotide Reductase written by Holly Kae Hurd and published by . This book was released on 1991 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Transcriptional Regulation of One-carbon Metabolism Genes of Saccharomyces Cerevisiae by : Seung-Pyo Hong
Download or read book Transcriptional Regulation of One-carbon Metabolism Genes of Saccharomyces Cerevisiae written by Seung-Pyo Hong and published by . This book was released on 1999 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Transcriptional Regulation of Ribosomal Protein Genes in Saccharomyces Cerevisiae by : Romeo Franciscus Lascaris
Download or read book Transcriptional Regulation of Ribosomal Protein Genes in Saccharomyces Cerevisiae written by Romeo Franciscus Lascaris and published by . This book was released on 2001 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of Yeast Transcription Factors Sko1 and Cst6 by Sumoylation by : Veroni Saratha Sri Theivakadadcham
Download or read book Regulation of Yeast Transcription Factors Sko1 and Cst6 by Sumoylation written by Veroni Saratha Sri Theivakadadcham and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sumoylation is a post-translational modification that plays an essential role in cellular processes, including transcriptional regulation. Transcription factors represent one of the largest groups of proteins that are modified by the SUMO (Small Ubiquitin-like Modifier) protein. In this study, we focused on finding roles for sumoylation in regulating two gene-specific bZIP transcription factors, Sko1 and Cst6, in Saccharomyces cerevisiae. Sko1 plays a unique bifunctional role in regulating transcription: it is a repressor during normal growth, by interacting with co-repressor complexes, and an activator during osmotic stress, via interaction with Hog1 Kinase. We show that Sko1 is poly-sumoylated at Lys 567 but its sumoylation is not regulated by stress. Along with sumoylation, Sko1 also undergoes phosphorylation, by PKA and Hog1, and our experiments show that these two modifications are not interdependent. We find that DNA binding is a requirement for Sko1 sumoylation and genome-wide chromatin immunoprecipitation (ChIP-seq) analysis shows that Sko1 sumoylation controls the occupancy level of Sko1 on target promoters and is involved in preventing Sko1 from binding to non-target promoters. Moreover, blocking sumoylation attenuated the interaction between Sko1 and Hog1 on target promoters. Cst6, on the other hand, is required for survival during ethanol stress and has roles in the utilization of carbon sources other than glucose. In this study, we show that Cst6 is multi-sumoylated at Lys residues 139, 461 and 547. The level of Cst6 sumoylation increases in ethanol and oxidative stress conditions, but decreases if ethanol is used as the sole carbon source. Unlike Sko1, protein levels of SUMO-deficient Cst6 were moderately reduced compared to the wild-type form, implying that sumoylation promotes Cst6 stability. ChIP experiments suggests that sumoylation is important for the timely recruitment of Cst6 to its target promoters. In addition, we provide evidence that Cst6 sumoylation reduces the expression of some target genes, during non-stress and ethanol stress conditions. Taken together, our studies suggest that the specific effects of sumoylation in regulating transcription factors are target specific. Nevertheless, SUMO plays a general role by controlling the transcription factor-DNA interaction to maintain proper gene expression.
Book Synopsis Transcriptional Regulation by War1p in Saccharomyces Cerevisiae by : Laila Beynon
Download or read book Transcriptional Regulation by War1p in Saccharomyces Cerevisiae written by Laila Beynon and published by . This book was released on 2008 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mechanisms of Transcriptional Control in Phosphate-responsive Signaling Pathway of Saccharomyces Cerevisiae by : Xu Zhou
Download or read book Mechanisms of Transcriptional Control in Phosphate-responsive Signaling Pathway of Saccharomyces Cerevisiae written by Xu Zhou and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of gene expression is essential for many biological processes. Binding of transcription factors to DNA is a key regulatory step in the control of gene expression. It is commonly observed that DNA sequences with high affinity for transcription factors occur more frequently in the genome than the instances of genes bound or regulated by these factors. However, the mechanism by which transcription factors selectively identify and regulate these genes was unclear. I utilized the transcriptional control of the phosphate-responsive signaling pathway (PHO) in Saccharomyces cerevisiae as a model system to address this problem.
Book Synopsis The Role of RAP1 and GCR1 in the Transcriptional Regulation of the Saccharomyces Cerevisiae PGK Gene by : Yves Andre Louis Henry
Download or read book The Role of RAP1 and GCR1 in the Transcriptional Regulation of the Saccharomyces Cerevisiae PGK Gene written by Yves Andre Louis Henry and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Connections Between Growth and Division by : Laura Lynn Newcomb
Download or read book Connections Between Growth and Division written by Laura Lynn Newcomb and published by . This book was released on 2001 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
