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Author : Joseph A. Coppola
Publisher :
ISBN 13 :
Total Pages : 132 pages
Book Rating : 4.:/5 (121 download)
Download or read book Transcription Initiation by RNA Polymerase II in Vitro written by Joseph A. Coppola and published by . This book was released on 1984 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : George A. Jacob
Publisher :
ISBN 13 :
Total Pages : 298 pages
Book Rating : 4.:/5 (355 download)
Download or read book An in Vitro Analysis of Transcription Initiation at Three RNA Polymerase II Promoters written by George A. Jacob and published by . This book was released on 1996 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Catherine Patricia George
Publisher :
ISBN 13 :
Total Pages : 232 pages
Book Rating : 4.:/5 (318 download)
Download or read book Mechanisms of RNA Polymerase II Transcription Initiation written by Catherine Patricia George and published by . This book was released on 1996 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : J.M. Walker
Publisher : Springer Science & Business Media
ISBN 13 : 9401165637
Total Pages : 304 pages
Book Rating : 4.4/5 (11 download)
Download or read book Techniques in Molecular Biology written by J.M. Walker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Author : Robert J. White
Publisher : Springer Science & Business Media
ISBN 13 : 3662035189
Total Pages : 274 pages
Book Rating : 4.6/5 (62 download)
Download or read book RNA Polymerase III Transcription written by Robert J. White and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews and summarizes the substantial body of work that has been published on the transcription by polymerase III over the past 5 years. Progress in this field has been very rapid since 1993, and this new edition incorporates all the recent developments and offers the reader a highly detailed analysis of the current state of research on this largest and most complex of the eukaryotic RNA polymerases.
Author : Stephen Buratowski
Publisher :
ISBN 13 :
Total Pages : 182 pages
Book Rating : 4.:/5 (24 download)
Download or read book Transcription Initiation by RNA Polymerase II written by Stephen Buratowski and published by . This book was released on 1990 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : David Bunick
Publisher :
ISBN 13 :
Total Pages : 252 pages
Book Rating : 4.:/5 (137 download)
Download or read book Studies of the Specific Initiation of RNA Polymerase II in Vitro written by David Bunick and published by . This book was released on 1982 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sharon Lynn Wampler
Publisher :
ISBN 13 :
Total Pages : 170 pages
Book Rating : 4.:/5 (318 download)
Download or read book A Biochemical Analysis of Transcription Initiation by RNA Polymerase II written by Sharon Lynn Wampler and published by . This book was released on 1993 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Stephen Peter Bell
Publisher :
ISBN 13 :
Total Pages : 368 pages
Book Rating : 4.:/5 (33 download)
Download or read book Mechanisms of Species Specific Transcriptional Initiation by RNA Polymerase I written by Stephen Peter Bell and published by . This book was released on 1990 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Martin J. Tymms
Publisher : Springer Science & Business Media
ISBN 13 : 1592595243
Total Pages : 426 pages
Book Rating : 4.5/5 (925 download)
Download or read book In Vitro Transcription and Translation Protocols written by Martin J. Tymms and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.
Author : Sankar Adhya
Publisher : Elsevier
ISBN 13 : 0080522599
Total Pages : 833 pages
Book Rating : 4.0/5 (85 download)
Download or read book RNA Polymerase and Associated Factors, Part C written by Sankar Adhya and published by Elsevier. This book was released on 2003-12-18 with total page 833 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
Author : Huiyan Jin
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (961 download)
Download or read book Influence of RNA Polymerase II Catalytic Activity on Transcription Start Site Selection written by Huiyan Jin and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Polymerase II (Pol II) is responsible for expression of all protein-coding genes in eukaryotes. To understand gene expression at the molecular level, it is essential to understand the mechanisms of Pol II function. I investigate how Pol II catalytic activity influences the first step of gene expression, transcription initiation, in Saccharomyces cerevisiae. My dissertation focuses on the mechanisms by which Pol II activity defects contribute to transcription start site (TSS) selection/utilization and promoter output. I utilize mutants with substitutions in the Pol II active site that have different elongation rates in vitro and show varied growth phenotypes in vivo that correlate with the observed alterations in elongation rates. I employ genetic and biochemical approaches to investigate the relationships between the TSS phenotypes of Pol II activity/General Transcription Factor (GTF) mutants and conditional and general growth phenotypes. I show that while some conditional growth phenotypes correlate closely with TSS defects, TSS defects are not likely the main determinant for general growth defects. I further explore growth phenotypes and TSS defects between Pol II genetic interactors and GTF mutants combined with Pol II activity mutants and present different models for the relationships discovered so far. I discover a novel function of Sub1, which is suggested to play positive roles in transcription as an initiation factor, altering TSS utilization on its own and modifying TSS defects conferred by Pol II activity mutants. I take a genome wide approach to map TSSs, general transcription factor occupancies, and nucleosome positions to investigate the mechanism of Pol II activity control over initiation, and determine how promoter architecture influences TSS selection/utilization and nucleosome positioning. I show that promoter architecture is a critical parameter in determination of various initiation properties including transcription output (gene expression) levels. The electronic version of this dissertation is accessible from http://hdl.handle.net/1969.1/155628
Author : William Shelley Dynan
Publisher :
ISBN 13 :
Total Pages : 378 pages
Book Rating : 4.:/5 (89 download)
Download or read book In Vitro Transcription by Wheat Germ RNA Polymerase II written by William Shelley Dynan and published by . This book was released on 1980 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Michael Justin Blau
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (61 download)
Download or read book The Regulation of Transcription Initiation and Elongation by RNA Polymerase II. written by Michael Justin Blau and published by . This book was released on 1996 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : J. Robin Harris
Publisher : Springer Nature
ISBN 13 : 3030281515
Total Pages : 657 pages
Book Rating : 4.0/5 (32 download)
Download or read book Macromolecular Protein Complexes II: Structure and Function written by J. Robin Harris and published by Springer Nature. This book was released on 2020-01-14 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author : Nam Viet Vo
Publisher :
ISBN 13 :
Total Pages : 248 pages
Book Rating : 4.:/5 (34 download)
Download or read book In Vitro Studies of the Transcript Initiation Process by E. Coli RNA Polymerase written by Nam Viet Vo and published by . This book was released on 1998 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jason Nicholas Kuehner
Publisher :
ISBN 13 :
Total Pages : 188 pages
Book Rating : 4.:/5 (891 download)
Download or read book Mechanism and Regulation of Yeast RNA Polymerase II Transcription Initiation and Termination written by Jason Nicholas Kuehner and published by . This book was released on 2008 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
