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Toward Multiplexed Nucleic Acid Assays And Biosensors Using Immobilized Quantum Dots As Donors In Fluorescence Resonance Energy Transfer Fret
Download Toward Multiplexed Nucleic Acid Assays And Biosensors Using Immobilized Quantum Dots As Donors In Fluorescence Resonance Energy Transfer Fret full books in PDF, epub, and Kindle. Read online Toward Multiplexed Nucleic Acid Assays And Biosensors Using Immobilized Quantum Dots As Donors In Fluorescence Resonance Energy Transfer Fret ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Toward Multiplexed Nucleic Acid Assays and Biosensors Using Immobilized Quantum Dots as Donors in Fluorescence Resonance Energy Transfer (FRET). by : Walter Russell Algar
Download or read book Toward Multiplexed Nucleic Acid Assays and Biosensors Using Immobilized Quantum Dots as Donors in Fluorescence Resonance Energy Transfer (FRET). written by Walter Russell Algar and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis FRET - Förster Resonance Energy Transfer by : Igor L. Medintz
Download or read book FRET - Förster Resonance Energy Transfer written by Igor L. Medintz and published by John Wiley & Sons. This book was released on 2013-10-17 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.
Book Synopsis Nanobiosensors and Nanobioanalyses by : Mun'delanji C. Vestergaard
Download or read book Nanobiosensors and Nanobioanalyses written by Mun'delanji C. Vestergaard and published by Springer. This book was released on 2015-03-18 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive review of established, cutting-edge, and future trends in the exponentially growing field of nanomaterials and their applications in biosensors and bioanalyses. Part I focuses on the key principles and transduction approaches, reviewing the timeline featuring the important historical milestones in the development and application of nanomaterials in biosensors and bioanalyses. Part II reviews various architectures used in nanobiosensing designs focusing on nanowires, one- and two-dimensional nanostructures, and plasmonic nanobiosensors with interferometric reflectance imaging. Commonly used nanomaterials, functionalization of the nanomaterials, and development of nanobioelectronics are discussed in detail in Part III with examples from screen-printed electrodes, nanocarbon films, and semiconductor quantum dots. Part IV reviews the current applications of carbon nanotubes, nanoneedles, plasmonic sensors, electrochemical scanning microscopes, and field-effect transistors with the future outlook for emerging technologies. Attention is also given to potential challenges, in particular, of taking these technologies at the point-of-need. The book concludes by providing a condensed summary of the contents, with emphasis on future directions. Nanomaterials have become an essential part of biosensors and bioanalyses in the detection and monitoring of medical, pharmaceutical, and environmental conditions, from cancer to chemical warfare agents. This book, with its distinguished editors and international team of expert contributors, will be an essential guide for all those involved in the research, design, development, and application of nanomaterials in biosensors and bioanalyses.
Book Synopsis Towards Solid-phase Nucleic Acid Hybridization Assays on a Paper-based Platform and Within Channel Microfluidics Using Quantum Dots as Donors in Fluorescence Resonance Energy Transfer by : Muhammad Omair Noor
Download or read book Towards Solid-phase Nucleic Acid Hybridization Assays on a Paper-based Platform and Within Channel Microfluidics Using Quantum Dots as Donors in Fluorescence Resonance Energy Transfer written by Muhammad Omair Noor and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Quantum Dots for DNA Biosensing by : Jun-Jie Zhu
Download or read book Quantum Dots for DNA Biosensing written by Jun-Jie Zhu and published by Springer Science & Business Media. This book was released on 2013-12-04 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a broad introduction to all major aspects of quantum dot properties including fluorescence, electrochemical, photochemical and electroluminescence. Such properties have been produced for applications in biosensing, cell tracking, in vivo animal imaging and so on. It focuses on their special applications in DNA biosensing and provides readers with detailed information on the preparation and functionalization of quantum dots and the fabrication of DNA biosensors, using examples to show how these properties can be used in DNA biosensor design and the advantages of quantum dots in DNA biosensing. Further new emerging quantum dots such as metal nanoclusters and graphene dots and their applications in DNA biosensing have also been included.
Book Synopsis Towards Multi-colour Strategies for the Detection of Nucleic Acid Hybridization Using Quantum Dots as Energy Donors in Fluorescence Resonance Energy Transfer (FRET). by : Walter Russell Algar
Download or read book Towards Multi-colour Strategies for the Detection of Nucleic Acid Hybridization Using Quantum Dots as Energy Donors in Fluorescence Resonance Energy Transfer (FRET). written by Walter Russell Algar and published by . This book was released on 2006 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The unique optical properties of quantum dots (QDs) are of interest in the development of nucleic acid diagnostics. The potential for a simultaneous two-colour diagnostic scheme for nucleic acids operating on the basis of fluorescence resonance energy transfer (FRET) has been demonstrated. Upon ultraviolet excitation, two-colours of CdSe/ZnS quantum dots with conjugated oligonucleotide probes acted as energy donors yielding FRET-sensitized acceptor emission upon hybridization with fluorophore labeled target oligonucleotides. The use of an intercalating dye to improve signal-to-noise was also demonstrated. The major limitation of the system was the non-specific adsorption of oligonucleotides, which was characterized extensively. Adsorptive interactions were found to affect the conformation of oligonucleotides conjugated to QDs, the kinetics of hybridization with QD-DNA conjugates, and the thermal stability of those hybrids. In addition, it was found that thiol-alkyl-acid capped QDs exhibited pKa correlated ligand-chromism and radiative decay rate-driven changes in quantum yield.
Book Synopsis Towards The Development Of A Quantum Dot Based Bioprobe For Intracellular Investigations of Nucleic Acid Hybridization Events Using Fluorescence Resonance Energy Transfer by : Lori Lok-Yin Chong
Download or read book Towards The Development Of A Quantum Dot Based Bioprobe For Intracellular Investigations of Nucleic Acid Hybridization Events Using Fluorescence Resonance Energy Transfer written by Lori Lok-Yin Chong and published by . This book was released on 2011 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: The unique spectroscopic properties of quantum dots (QDs) are of interest for application in intracellular studies of gene expression. QDs derivatized with single-stranded probe oligonucleotides were used to detect complementary target sequences via hybridization and fluorescence resonance energy transfer (FRET). As nucleic acid targets are not labeled within cells, a displacement assay for nucleic acid detection featuring QDs as FRET donors was developed. QDs conjugated with oligonucleotide probes and then pre-hybridized with labeled target yielded efficient FRET in vitro. Studies in vitro confirmed that displacement kinetics of pre-hybridized target was a function of the stability of the initial hybridized complex. Displacement was observed as reduction in FRET intensity coupled with regeneration of QD fluorescence. By engineering the sequence of the labeled target, faster displacement was possible. The QD-probe+target system was successfully delivered into cells via transfection. Although QDs with their cargo remained sequestered in endosomal vesicles, fluorescent properties were retained.
Book Synopsis Towards a Microfluidics-based Nucleic Acid Biosensor Using Immobilized Quantum Dot - DNA Conjugates for FRET Detection of Target Oligonucleotides by : Jonathan Cauchi
Download or read book Towards a Microfluidics-based Nucleic Acid Biosensor Using Immobilized Quantum Dot - DNA Conjugates for FRET Detection of Target Oligonucleotides written by Jonathan Cauchi and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The potential for an electrokinetically driven, FRET-based microfluidic biosensor for SNP discrimination has been explored. The method functionalized the glass wall of a PDMS/glass microfluidics channel with multidentate thiol ligands to noncovalently immobilize MPA capped CdSe/ZnS QDs. Single stranded probe DNA could then be immobilized to QDs and target material could be delivered electrokinetically to the sensing surface. SNP discrimination could then occur by manipulation of shear, electrical and thermal effects derived from the applied voltage. The stability of immobilized QDs was investigated by EOF experiments that applied 500 V and 100 V voltages for 10 minutes to initiate electrokintetic flow. Fluorescence intensity measurements showed nearly complete removal of QDs from slides when compared with controls at both voltages. Pressure driven flow experiments demonstrated reduced dissociation of immobilized of QDs compared to channels exposed to EOF. A covalent approach is likely necessary to ensure stability of immobilized QDs during EOF.
Book Synopsis Solid-phase On-chip Nucleic Acid Hybridization Assays Using Spatial Profiles from Fluorescence Resonance Energy Transfer by Immobilized Quantum Dots by : Anthony James Tavares
Download or read book Solid-phase On-chip Nucleic Acid Hybridization Assays Using Spatial Profiles from Fluorescence Resonance Energy Transfer by Immobilized Quantum Dots written by Anthony James Tavares and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The research that is presented herein explores the development of a solid-phase DNA hybridization assay in an electrokinetically controlled biochip. Transduction of nucleic acid hybridization is accomplished by fluorescence resonance energy transfer (FRET) from a layer of immobilized quantum dots (QDs) in microfluidic channels. The chip assay platform was assembled as a composite of glass and polydimethylsiloxane (PDMS), where the glass surface was functionalized with immobilization chemistry to support the spontaneous assembly of QDs. Probe oligonucleotides were subsequently conjugated to the immobilized QDs and hybridization served as the selective interaction for target binding. Since the entire microchannel was derivatized with the transduction element, hybridization of dye labeled oligonucleotides along the channel length created well defined spatial profiles of FRET sensitized acceptor emission. The length of the FRET spatial profiles was related to the quantity of nucleic acid delivered and enabled quantitative transduction of tens of fmol amounts of target within minutes. This chip based assay offered multiplexed analysis where the concurrent detection of two targets was possible with a dynamic range spanning more than an order of magnitude. The robustness of the assay was demonstrated by transduction of nucleic acid targets in a variety of complex matrices including sample solutions that contained an excess of genomic DNA and also serum proteins. Furthermore, the assay offered excellent selectivity toward determination of the presence of a single nucleotide polymorphism (SNP), with contrast ratios exceeding 100:1. A non-traditional approach to SNP transduction was explored, where the size of the QD was found to impact the stringency of interfacial hybridization. Detection of unlabeled target oligonucleotide using a sandwich assay approach enabled transduction of oligonucleotides up to 40 nucleotides in length. The research described herein advances the development of a selective interfacial transduction strategy for the detection of nucleic acid markers such as might be characteristic of disease or pathogens. The on-chip assay format is amenable to point-of-care analysis and can be combined with nucleic acid amplification technologies for highly sensitive on demand DNA analysis.
Book Synopsis Quantum Dots and Upconverting Nanoparticles by : Shashi Bhuckory
Download or read book Quantum Dots and Upconverting Nanoparticles written by Shashi Bhuckory and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combining high sensitivity with simultaneous analysis of numerous biomarkers (multiplexing) is an essential requirement for significantly improving the field of biomedical diagnostics. Such progresses would allow earlier diagnosis, which is required for numerous diseases such as cancer or cardiac diseases. FRET-immunoassays are based on biomolecular recognition events that occur between biomarkers and two specific antibodies conjugated with different fluorophores. The spatial proximity of the two fluorophores can lead to Förster resonance energy transfer (FRET), which can be detected for biomarker quantification. To date, such assays are established using lanthanide complexes as FRET donors and fluorescence dyes as FRET acceptors. However, these assays do not provide sufficient multiplexing capability due to spectral overlap, when several acceptor dyes are used. This project aims at exploiting the exceptional photophysical properties of terbium complexes (Tb) and semiconductor quantum dots (QDs) to provide ultrasensitive multiplexed FRETimmunoassays. We also studied the optical and morphological properties of novel core and core/shell upconverting nanoparticles doped with ytterbium (Yb) and erbium (Er) ions as possible FRET-donors for biosensing.
Book Synopsis Time-resolved Multiplexed Förster Resonance Energy Transfer for Nucleic Acid Biosensing by : Jiajia Guo
Download or read book Time-resolved Multiplexed Förster Resonance Energy Transfer for Nucleic Acid Biosensing written by Jiajia Guo and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acid biomarkers, which involve in gene expression control, are found specific for many kinds of cancers. Förster Resonance Energy Transfer (FRET) based applications are one of the most promising for nucleic acid biosensing. As parallel detection of multiple nucleic acids is highly demanded and spectral multiplexing is limited by optical crosstalk, temporal multiplexing is used for opening another dimension of the multiplexing. The thesis focuses on developing different Tb-to-dye FRET distances to create specific intensity signals corresponding to different nucleic acid sequences. The Tb-dye distances can be tuned by specific location of the Tb donor using different lengths of DNA. Amplification technologies, such as hybridization chain reaction (HCR) and rolling circle amplification (RCA), are used to achieve simplicity, rapidity, selectivity, and sensitivity of nucleic acid detection. Temporal multiplexing FRET was also combined with spectral (color) multiplexing for higher order multiplexed detection. Moreover, a single Tb-QD FRET modeling demonstrated the possibility of nanoparticle-based temporal multiplexing.
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2720 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Nanobiosensors by : Alexandru Grumezescu
Download or read book Nanobiosensors written by Alexandru Grumezescu and published by Academic Press. This book was released on 2016-09-28 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanobiosensors: Nanotechnology in the Agri-Food Industry, Volume 8, provides the latest information on the increasing demand for robust, rapid, inexpensive, and safe alternative technologies that monitor, test, and detect harmful or potentially dangerous foods. Due to their high sensitivity and selectivity, nanobiosensors have attracted attention for their use in monitoring not only biological contaminants in food, but also potential chemical and physical hazards. This book offers a broad overview regarding the current progress made in the field of nanosensors, including cutting-edge technological progress and the impact of these devices on the food industry. Special attention is given to the detection of microbial contaminants and harmful metabolotes, such as toxins and hormones, which have a great impact on both humans and animal health and feed. Includes the most up-to-date information on nanoparticles based biosensors and quantum dots for biological detection Provides application methods and techniques for research analysis for bacteriological detection and food testing Presents studies using analytical tools to improve food safety and quality analysis
Book Synopsis Toward the Development of Nucleic Acid Assays Using Fluorescence Resonance Energy Transfer (FRET) and a Novel Label Free Molecular Switching Construct by : Melissa Massey
Download or read book Toward the Development of Nucleic Acid Assays Using Fluorescence Resonance Energy Transfer (FRET) and a Novel Label Free Molecular Switching Construct written by Melissa Massey and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Quantum Dot-fluorescent Protein Pairs as Fluorescence Resonance Energy Transfer Pairs by : Allison Marie Dennis
Download or read book Quantum Dot-fluorescent Protein Pairs as Fluorescence Resonance Energy Transfer Pairs written by Allison Marie Dennis and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence resonance energy transfer (FRET)-based biosensors have been designed to fluorometrically detect everything from proteolytic activity to receptor-ligand interactions and structural changes in proteins. While a wide variety of fluorophores have demonstrated effectiveness in FRET probes, several potential sensor components are particularly notable. Semiconductor quantum dots (QDs) are attractive FRET donors because they are rather bright, exhibit high quantum yields, and their nanoparticulate structure enables the attachment of multiple acceptor molecules. Fluorescent proteins (FPs) are also of particular interest for fluorescent biosensors because design elements necessary for signal transduction, probe assembly, and device delivery and localization for intracellular applications can all be genetically incorporated into the FP polypeptide. : The studies described in this thesis elucidate the important parameters for concerted QD-FP FRET probe design. Experimental results clarify issues of FRET pair selection, probe assembly, and donor-acceptor distance for the multivalent systems. Various analysis approaches are compared and guidelines asserted based on the results. To demonstrate the effectiveness of the QD-FP FRET probe platform, a ratiometric pH sensor is presented. The sensor, which uses the intrinsic pH-sensitivity of the FP mOrange to modulate the FP/QD emission ratio, exhibits a 20-fold change in its ratiometric measurement over a physiologically interesting pH range, making it a prime candidate for intracellular imaging applications.
Author :Melissa Massey Publisher :Library and Archives Canada = Bibliothèque et Archives Canada ISBN 13 :9780494021569 Total Pages :368 pages Book Rating :4.0/5 (215 download)
Book Synopsis Towards a Fluorescence Resonance Energy Transfer (FRET) Strategy for Development of a Biosensor for Detection of Nucleic Acid Hybridization on Fused Silica Surfaces [microform] by : Melissa Massey
Download or read book Towards a Fluorescence Resonance Energy Transfer (FRET) Strategy for Development of a Biosensor for Detection of Nucleic Acid Hybridization on Fused Silica Surfaces [microform] written by Melissa Massey and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2005 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thiazole orange (TO) with different tethers were synthesized to be attached to oligonucleotides. The FRET of TO in solution with double-stranded DNA (dsDNA) was investigated with BlackHole (BHQ1) or ((4-dimethylamino)phenyl)azo)benzoic acid (DABCYL) quenchers which decreased the fluorescence 2.9 +/- 7% and 2.5 +/- 10% times, respectively. A quenching mechanism could therefore be designed to transduce hybridization. The FRET of N, N, N, N-tetramethylcarboxyrhodamine (TAMRA) and IowaBlackRQ RTM (IABLK) linked to complementary oligonucleotides immobilized on glass substrates was investigated; IABLK quenched TAMRA fluorescence. However, surface bound dsDNA caused some self-quenching of TAMRA. Solution FRET using TAMRA/IABLK at 24.5°C and 60°C with complementary and mismatched DNA was measured to investigate potential for mismatch detection. The probe sequence was based on the determinate for Spinal Muscular Atrophy (SMA). Signal intensity differed between complementary and the mismatch samples at 60°C, indicating mismatch detection potential. Results suggest the possibility of designing tethered fluorophore-quencher pairs for transduction of hybridization for development of optical nucleic acid biosensors for SMA screening.
Book Synopsis Biomedical Sensors by : Deric P. Jones
Download or read book Biomedical Sensors written by Deric P. Jones and published by Momentum Press. This book was released on 2010 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sensors are the eyes, ears, and more, of the modern engineered product or system- including the living human organism. This authoritative reference work, part of Momentum Press's new Sensors Technology series, edited by noted sensors expert, Dr. Joe Watson, will offer a complete review of all sensors and their associated instrumentation systems now commonly used in modern medicine. Readers will find invaluable data and guidance on a wide variety of sensors used in biomedical applications, from fluid flow sensors, to pressure sensors, to chemical analysis sensors. New developments in biomaterials- based sensors that mimic natural bio-systems will be covered as well. Also featured will be ample references throughout, along with a useful Glossary and symbols list, as well as convenient conversion tables.
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