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The Science Of Biological Specimen Preparation For Microscopy
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Book Synopsis Biological Specimen Preparation for Transmission Electron Microscopy by : Audrey M. Glauert
Download or read book Biological Specimen Preparation for Transmission Electron Microscopy written by Audrey M. Glauert and published by Princeton University Press. This book was released on 2014-07-14 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Book Synopsis Sample Preparation Handbook for Transmission Electron Microscopy by : Jeanne Ayache
Download or read book Sample Preparation Handbook for Transmission Electron Microscopy written by Jeanne Ayache and published by Springer Science & Business Media. This book was released on 2010-07-03 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Book Synopsis The science of biological specimen preparation for microscopy and microanalysis by :
Download or read book The science of biological specimen preparation for microscopy and microanalysis written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy by : Marek Malecki
Download or read book The Science of Biological Specimen Preparation for Microscopy written by Marek Malecki and published by . This book was released on 1996 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Sample Preparation Handbook for Transmission Electron Microscopy by : Jeanne Ayache
Download or read book Sample Preparation Handbook for Transmission Electron Microscopy written by Jeanne Ayache and published by Springer Science & Business Media. This book was released on 2010-06-08 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis by : Jean-Paul Revel
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis written by Jean-Paul Revel and published by . This book was released on 1984 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis by : Martin Müller
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis written by Martin Müller and published by . This book was released on 1986 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis 1990 by : Ludwig Edelmann
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis 1990 written by Ludwig Edelmann and published by . This book was released on 1991 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis by : Jean-Paul Revel
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis written by Jean-Paul Revel and published by . This book was released on 1984 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis by :
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis written by and published by . This book was released on 1984 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis 1990 by : Ludwig Edelmann
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis 1990 written by Ludwig Edelmann and published by . This book was released on 1991 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis by : Jean-Paul Revel
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis written by Jean-Paul Revel and published by . This book was released on 1986 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods of Preparation for Electron Microscopy by : David G. Robinson
Download or read book Methods of Preparation for Electron Microscopy written by David G. Robinson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis 1985 by : Martin Müller
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis 1985 written by Martin Müller and published by . This book was released on 1986 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Preparation of Biological Specimens for Scanning Electron Microscopy by : Judith A. Murphy
Download or read book Preparation of Biological Specimens for Scanning Electron Microscopy written by Judith A. Murphy and published by . This book was released on 1984 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis by : Patrick Echlin
Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Book Synopsis The Science of Biological Specimen Preparation for Microscopy and Microanalysis, 1988 by : R. M. Albrecht
Download or read book The Science of Biological Specimen Preparation for Microscopy and Microanalysis, 1988 written by R. M. Albrecht and published by . This book was released on 1989 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
