Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download The Role Of Oskar Mrna In P Body Formation During Drosophila Oogenesis full books in PDF, epub, and Kindle. Read online The Role Of Oskar Mrna In P Body Formation During Drosophila Oogenesis ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : Branislava Ranković
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (123 download)
Download or read book The Role of Oskar MRNA in P-body Formation During Drosophila Oogenesis written by Branislava Ranković and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Yung S. Lie
Publisher :
ISBN 13 :
Total Pages : 390 pages
Book Rating : 4.F/5 ( download)
Download or read book Translational Regulation of Oskar Mrna During Drosophila Oogenesis written by Yung S. Lie and published by . This book was released on 2000 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Vitaly Leonidovich Zimyanin
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (89 download)
Download or read book Analysis of the Molecular Mechanisms Underlying Oskar MRNA Localisation and Axis Formation During Drosophila Oogenesis written by Vitaly Leonidovich Zimyanin and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 81 pages
Book Rating : 4.:/5 (528 download)
Download or read book Spatial and Temporal Coordination of Oskar MRNA Localization and Translation During Drosophila Oogenesis written by and published by . This book was released on 2003 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Young Hee Ryu
Publisher :
ISBN 13 :
Total Pages : 278 pages
Book Rating : 4.:/5 (986 download)
Download or read book The Role of Bicoid Stability Factor in Oskar MRNA Function and Regulation, and the Mechanisms for Oskar MRNA Transport to the Oocyte written by Young Hee Ryu and published by . This book was released on 2015 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: My dissertation is separated into two subjects. First, I examined the role of Bicoid Stability Factor (BSF) in oskar (osk) regulation. Second, I studied cis-acting elements involved in osk mRNA transport to the oocyte during early oogenesis. Oskar (Osk) is a body patterning determinant in Drosophila and is highly concentrated at the posterior pole of the oocyte. This spatially-restricted deployment relies on a coordinated program of osk mRNA localization and translational regulation, all dependent on cis-acting regulatory elements located primarily in the 3’ UTR of the mRNA. Notably, some of these elements, as well as sequences required for a noncoding role of osk mRNA, are clustered in a short region (the C region) near the 3’ end of the osk mRNA. To better understand the role of the C region, I searched for proteins that bind specifically to this region and I found BSF. Binding assays to mutant RNAs suggested that BSF does not act in the noncoding function of osk mRNA. To test for a role for BSF in regulation of Osk protein expression, I used two complementary approaches, reducing the BSF level or disrupting BSF binding to the osk mRNA. Both generated similar results: a reduction or loss of posterior Osk protein and osk mRNA in late oogenesis and early embryogenesis, while the level of osk mRNA was not affected. My work suggests that BSF could act in a late phase of osk mRNA localization or translational activation. Localization of osk mRNA to the posterior pole of the oocyte is achieved by multiple transport steps. One is mRNA transport from the nurse cells to the oocyte. Although cis-acting elements including the oocyte entry signal (OES) in the osk mRNA 3’ UTR have been implicated in mRNA oocyte transport, the precise mechanisms remain unknown. Here, I show that the clusters of Bru binding sites in the osk mRNA 3’ UTR required for translational regulation confer oocyte transport on a reporter mRNA. However, neither Bru sites nor the OES are essential for oocyte transport of osk mRNA. This suggests that there are multiple mechanisms redundantly acting in oocyte transport.
Author : Youme Gim
Publisher :
ISBN 13 :
Total Pages : 40 pages
Book Rating : 4.:/5 (96 download)
Download or read book Bruno Contributes to Osk MRNA Localization During Drosophila Oogenesis written by Youme Gim and published by . This book was released on 2011 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oskar is a body pattern and germ cell determining protein in Drosophila melanogaster. This protein must be properly expressed to ensure correct formation of the posterior pole of the animal. Bruno is a RNA binding protein known to regulate osk mRNA translation during Drosophila oogenesis. In vitro work has implicated Bru in oligomerizing osk mRNA into silencing particles and thereby preventing osk mRNA from accessing translational machinery and inhibiting translation. In attempts to further investigate Bru mediated translational regulation, reporter transcripts with Bruno binding regions from the osk mRNA were analyzed for translation and localization of the transcript. Localization of these reporter transcripts has shown the first in vivo evidence for the Bru mediated silencing particle assembly model. In this thesis, I report on the distribution of reporter transcripts in the Drosophila egg chamber.
Author : Nahum Sonenberg
Publisher : CSHL Press
ISBN 13 : 9780879696184
Total Pages : 1034 pages
Book Rating : 4.6/5 (961 download)
Download or read book Translational Control of Gene Expression written by Nahum Sonenberg and published by CSHL Press. This book was released on 2001 with total page 1034 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control.
Author : Florence Louise Marlow
Publisher : Morgan & Claypool Publishers
ISBN 13 : 161504051X
Total Pages : 221 pages
Book Rating : 4.6/5 (15 download)
Download or read book Maternal Control of Development in Vertebrates written by Florence Louise Marlow and published by Morgan & Claypool Publishers. This book was released on 2010 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eggs of all animals contain mRNAs and proteins that are supplied to or deposited in the egg as it develops during oogenesis. These maternal gene products regulate all aspects of oocyte development, and an embryo fully relies on these maternal gene products for all aspects of its early development, including fertilization, transitions between meiotic and mitotic cell cycles, and activation of its own genome. Given the diverse processes required to produce a developmentally competent egg and embryo, it is not surprising that maternal gene products are not only essential for normal embryonic development but also for fertility. This review provides an overview of fundamental aspects of oocyte and early embryonic development and the interference and genetic approaches that have provided access to maternally regulated aspects of vertebrate development. Some of the pathways and molecules highlighted in this review, in particular, Bmps, Wnts, small GTPases, cytoskeletal components, and cell cycle regulators, are well known and are essential regulators of multiple aspects of animal development, including oogenesis, early embryogenesis, organogenesis, and reproductive fitness of the adult animal. Specific examples of developmental processes under maternal control and the essential proteins will be explored in each chapter, and where known conserved aspects or divergent roles for these maternal regulators of early vertebrate development will be discussed throughout this review. Table of Contents: Introduction / Oogenesis: From Germline Stem Cells to Germline Cysts / Oocyte Polarity and the Embryonic Axes: The Balbiani Body, an Ancient Oocyte Asymmetry / Preparing Developmentally Competent Eggs / Egg Activation / Blocking Polyspermy / Cleavage/ Mitosis: Going Multicellular / Maternal-Zygotic Transition / Reprogramming: Epigenetic Modifications and Zygotic Genome Activation / Dorsal-Ventral Axis Formation before Zygotic Genome Activation in Zebrafish and Frogs / Maternal TGF-β and the Dorsal-Ventral Embryonic Axis / Maternal Control After Zygotic Genome Activation / Compensation by Stable Maternal Proteins / Maternal Contributions to Germline Establishment or Maintenance / Perspective / Acknowledgments / References
Author : Jean-Baptiste Coutelis
Publisher :
ISBN 13 :
Total Pages : 226 pages
Book Rating : 4.:/5 (78 download)
Download or read book Vesicular Traffic in Axis Specification During Drosophila Melanogaster Oogenisis written by Jean-Baptiste Coutelis and published by . This book was released on 2006 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: DROSOPHILA MELANOGASTER BODY AXES ARE DEFINED BY PRECISE LOCALIZATION AND RESTRICTION OF MOLECULAR DETERMINANTS IN THE OOCYTE. POLARIZATION OF THE OOCYTE DURING OOGENESIS IS CRITICAL FOR THIS PROCESS. THE DIRECTED TRAFFIC OF MEMBRANES AND PROTEINS IS A CRUCIAL COMPONENT OF POLARITY ESTABLISHMENT IN VARIOUS CELL TYPES AND ORGANISMS. DURING MY PHD WORK, I INVESTIGATED THE ROLE OF THE SMALL MONOMERIC GTPASE RAB6 IN ORGANIZATION OF THE EGG-CHAMBER AND ASYMMETRIC LOCALIZATION OF THE BODY AXIS DETERMINANTS DURING OOGENESIS. I FOUND THAT ABSENCE OF RAB6 FUNCTION CAUSES EGG-CHAMBER DISORGANIZATION AND LOSS OF NURSE CELL PLASMA MEMBRANES. I ALSO OBSERVED THAT EXOCYTOSIS IS AFFECTED IN RAB6 NULL EGG-CHAMBERS, PROVIDING AN EXPLANATION FOR THE OBSERVED PHENOTYPES. RAB6 IS ALSO REQUIRED FOR POLARIZATION OF THE OOCYTE MICROTUBULE CYTOSKELETON AND LOCALIZATION OF OSKAR MRNA AT THE OOCYTE POSTERIOR POLE. FURTHERMORE, I SHOWED THAT, IN VIVO, RAB6 IS FOUND IN A COMPLEX WITH BICAUDAL-D, KNOWN FOR ITS ROLE IN MRNA LOCALIZATION IN DROSOPHILA. GENETIC ANALYSIS REVEALS THAT, IN THE OOCYTE, RAB6 AND BICAUDAL-D COOPERATE IN OSKAR MRNA LOCALIZATION. AS A CONCLUSION, I PROPOSE THAT DURING DROSOPHILA OOGENESIS, RAB6-DEPENDENT MEMBRANE TRAFFICKING IS DOUBLY REQUIRED, FIRST FOR GENERAL ORGANIZATION AND GROWTH OF THE EGG- CHAMBER, AND SECOND, MORE SPECIFICALLY, FOR POLARIZATION OF THE MICROTUBULE CYTOSKELETON AND LOCALIZATION OF OSKAR MRNA. THESE FINDINGS HIGHLIGHT THE CENTRAL ROLE OF VESICULAR TRAFFICKING IN ESTABLISHMENT OF POLARITY AND DETERMINANT LOCALIZATION IN DROSOPHILA.
Author : Howard D. Lipshitz
Publisher : R G Landes Company
ISBN 13 : 9781570592768
Total Pages : 322 pages
Book Rating : 4.5/5 (927 download)
Download or read book Localized RNAs written by Howard D. Lipshitz and published by R G Landes Company. This book was released on 1995 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Alan Trounson
Publisher : Cambridge University Press
ISBN 13 : 1107470293
Total Pages : 464 pages
Book Rating : 4.1/5 (74 download)
Download or read book Biology and Pathology of the Oocyte written by Alan Trounson and published by Cambridge University Press. This book was released on 2013-10-24 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human egg - the rarest and most rapidly aging cell in the body - is a topic of intense scientific study. Assisted reproduction clinics are constantly vying to improve success rates - choosing the best gametes is a key step in this process. This new edition of what one reviewer of the first edition described as 'possibly the definitive work on the oocyte' covers the development, biology and pathology of the oocyte, and technologies to manipulate, enhance and control fertility. These technologies are used to overcome infertility, avoid inherited diseases, and create genetically engineered embryos from stem cells and cloning. This progress would have been impossible without the myriad of scientific and technical developments covered in this book. The new edition is thoroughly updated and includes major new research on reprogramming, oocyte molecular development, cryopreservation and viability. We are in exciting times for the egg.
Author : Marie-Helene Verlhac
Publisher : John Wiley & Sons
ISBN 13 : 9780470687987
Total Pages : 516 pages
Book Rating : 4.6/5 (879 download)
Download or read book Oogenesis written by Marie-Helene Verlhac and published by John Wiley & Sons. This book was released on 2010-03-04 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oogenesis - the process by which female germ cells develop into mature eggs, or ova - is a complex process involving many important elements of developmental and cellular biology: from cell-cell interactions, complex signalling cascades, specialized cell cycles and cytoskeleton organization. Oocytes from various species (including clam, starfish, xenopus and mouse) are excellent model systems to study the biochemistry of cell division with important implications for basic and clinical research. This book describes the entire process of oogenesis in chronological order with contributions from leading international researchers and chapters covering medical and ethical considerations in oogenic biology. Topics include sex determination and gonadal development, control of meiotic chromosome pairing and homologous recombination, control of meiotic divisions and the remodelling of the oocyte into a totipotent zygote as well as medically-assisted reproduction. This volume is an essential resource for all students, researchers and clinicians in developmental and reproductive biology. Key features: Reaches beyond the study of simply meiosis to cover all aspects of oogenesis Synthesizes recent advances in the field, drawing on studies from different model species Chapter sequence designed to follow the time line in vivo Written by an international panel of expert researchers
Author : Malgorzata Kloc
Publisher : Springer
ISBN 13 : 331960855X
Total Pages : 505 pages
Book Rating : 4.3/5 (196 download)
Download or read book Oocytes written by Malgorzata Kloc and published by Springer. This book was released on 2017-08-02 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book combines the most recent knowledge on the maternal, i.e. oocyte/egg-specific, molecules and processes. The volume covers the most recent advances in a plethora of subjects such as: maternal transfer of immunity, localized RNAs functions and mechanisms of RNA localization, transcriptional repression of maternal messages, maternal inheritance and maternal role of CRISPR/Cas9-based genome editing, chromatin remodeling and epigenetic modifications, maternal function of nucleosomes, maternal mitochondria and energy supply, role of bacterial symbionts and their maternal transmission, acquisition of oocyte polarity and evolution of maternal effect genes, germ plasm and oosome origin and functions, mechanisms of oocyte activation and soma germ cells communication. Currently, no other book on the market combines such a comprehensive list of subjects in one volume. Moreover, the information provided is a cross-section through oocytes from various invertebrate and vertebrate species, which is another unique feature of this book. The readers, therefore, get a completely new and invaluable perspective on all covered subjects.
Author : Antonius M. VanDongen
Publisher : CRC Press
ISBN 13 : 142004415X
Total Pages : 368 pages
Book Rating : 4.4/5 (2 download)
Download or read book Biology of the NMDA Receptor written by Antonius M. VanDongen and published by CRC Press. This book was released on 2008-10-29 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: The NMDA receptor plays a critical role in the development of the central nervous system and in adult neuroplasticity, learning, and memory. Therefore, it is not surprising that this receptor has been widely studied. However, despite the importance of rhythms for the sustenance of life, this aspect of NMDAR function remains poorly studied. Written
Author : Diana P. Bratu
Publisher : Humana
ISBN 13 : 9781493928507
Total Pages : 0 pages
Book Rating : 4.9/5 (285 download)
Download or read book Drosophila Oogenesis written by Diana P. Bratu and published by Humana. This book was released on 2015-09-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author : Raymond L. Rodriguez
Publisher : Butterworth-Heinemann
ISBN 13 : 1483103080
Total Pages : 593 pages
Book Rating : 4.4/5 (831 download)
Download or read book Vectors written by Raymond L. Rodriguez and published by Butterworth-Heinemann. This book was released on 2014-05-20 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.
Author : Lynne E. Maquat
Publisher : Academic Press
ISBN 13 : 0080922074
Total Pages : 661 pages
Book Rating : 4.0/5 (89 download)
Download or read book RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay written by Lynne E. Maquat and published by Academic Press. This book was released on 2009-01-30 with total page 661 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression - Offers step-by-step lab instructions, including necessary equipment and reagents
