The Host Pathogen Interaction And Breeding And Genetics Of Resistance For Common Bacterial Blight Of Common Bean Phaseolus Vulgaris L Caused By Xanthomonas Campestris Pv Phaseoli And X C Pv Phaseoli Var Fuscans

Author : Michael Lennon O'Leary
Publisher :
ISBN 13 : 9780438289819
Total Pages : pages
Book Rating : 4.2/5 (898 download)

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Download or read book Bacterial Blights of Common Bean written by Michael Lennon O'Leary and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Common bean is a genetically diverse crop that is a staple food in many regions of the world. However, common bean is affected by numerous diseases that can negatively impact production. There are three major bacterial diseases of common bean: halo blight, caused by Pseudomonas syringae pv. phaseolicola (Psp), bacterial brown spot, caused by P. syringae pv. syringae (Pss), and common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Xap) and X. fuscans subsp. fuscans (Xff). Disease symptoms caused by Psp and Pss can be difficult to distinguish in certain common bean genotypes and under certain environmental conditions. Precise pathogen identification is important in terms of potential yield loss and the need for disease management. Symptoms with characteristics of both halo blight and bacterial brown spot were observed on a newly developed cultivar of dark red kidney beans in Wisconsin, and pseudomonads were recovered from all samples. To determine the etiology of this disease, a method of identification was developed that involved 1) PCR with primers specific for toxin genes utilized by Psp and Pss, 2) the ability to induce a hypersensitivity response in tobacco leaves, and 3) symptoms induced on common bean. When applied to the unusual disease phenotype on this dark red kidney variety most of these were atypical symptoms of halo blight. This method was then successfully applied to identify bacterial brown spot in disease samples from Oregon, and halo blight and bacterial brown spot in disease samples from Turkey. Xap and Xff strains induce identical symptoms on common bean but are genetically distinct species. Strains of Xap and Xff can be differentiated in culture by the production of a brown diffusible pigment by Xff strains. However, some Xap strains, particularly those that originate from East Africa, are more genetically related to Xff strains than to the globally predominant Xap strains, and show pathogenic variability on certain cultivars, e.g., Andean versus Middle American. To better understand the genetic diversity among Xap and Xff strains, the complete genome sequences of three globally predominant Xap strains, two East African Xap strains, three Xff strains, and one Xanthomonas spp. strain that is associated with common bean but not pathogenic were determined using long-read sequencing technology. Whole-genome alignment revealed differences in chromosome organization between Xap strains, East African Xap strains, and Xff strains; additionally, mobile element content is variable among these strains. Conserved genetic factors, e.g. conserved plasmids and type III secreted effector proteins, some of which may have been horizontally acquired by Xap and Xff strains, were identified. The brown diffusible pigment produced by Xff strains is the result of an oxidation product of homogentisic acid, which is referred to as pyomelanin. In the tyrosine catabolism pathway, homogentisic acid is produced by 4-hydroxyphenylpyruvate dioxygenase (HPPD) and converted to 4-maleylacetoacetate by homogentisate dioxygenase (HmgA). The complete genome sequences of Xap and Xff strains revealed that Xff strains have a conserved mutation in the hmgA gene, whereas Xap strains have an intact hmgA gene. HPPD deletion mutants in an Xff strain resulted in loss of pyomelanin (brown pigment) production, whereas deletion of hmgA in two Xap strains resulted in brown pigment production, making Xap colonies resemble those of wild-type Xff strains. Additionally, complementing a wild-type Xff strain with a functional hmgA gene eliminated visible pyomelanin accumulation. Finally, virulence of a Xff HPPD and a Xap hmgA deletion mutant was assessed on twelve bean cultivars, including both Andean and Middle American gene pools, with different levels of disease resistance to common bacterial blight. There was no significant differences in virulence between brown pigment mutants and wild-type strains.