The Chlamydomonas Sourcebook Introduction To Chlamydomonas And Its Laboratory Use

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The Chlamydomonas Sourcebook

Author : Elizabeth H. Harris
Publisher : Elsevier
ISBN 13 : 1483288609
Total Pages : 780 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Elizabeth H. Harris

Download or read book The Chlamydomonas Sourcebook written by Elizabeth H. Harris and published by Elsevier. This book was released on 2013-10-22 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: The green alga Chlamydomonas is widely used as an experimental model system for studies in cellular and molecular biology, and in particular plant molecular biology. This book is the only single modern compendium of information on its biology and in particular its molecular biology and genetics. Included in addition to much information on the basic biology is material of a very practical nature, namely, methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. Casual users as well as specialists will find the book to be useful in many ways. Key Features * Provides access to previously unpublished data from genetic analysis * Provides descriptions of mutant strains * Depicts summary tables comparing properties of different species and their mutant strains * Explains detailed methods for laboratory procedures of general utility * Furnishes comparisons of culture media * Presents lists of inhibitors, mutagens, and other additives to culture media * Assists with common laboratory problems such as contamination and storage of strains * Demonstrates protocols for laboratory demonstrations available for undergraduate teaching.

The Chlamydomonas Sourcebook

Author : Ursula Goodenough
Publisher : Elsevier
ISBN 13 : 0128224576
Total Pages : 460 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Ursula Goodenough

Download or read book The Chlamydomonas Sourcebook written by Ursula Goodenough and published by Elsevier. This book was released on 2023-02-24 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use, Volume One, Third Edition has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community (new mutant libraries, new omics studies, and potentially more information about different Chlamydomonas species in the environment). In addition to updates on molecular techniques and analysis of the sequenced genome, the book presents the latest in research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, and more. Provides the only complete reference on both the historical background and most up-to-date information and applications on Chlamydomonas Edited by current leaders in the area, continuing the expert insights established through the first two editions Includes best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and more Aids researchers with common laboratory problems such as contamination and details on properties of particular strains and mutants

The Chlamydomonas Sourcebook 3-Vol set

Author : David Stern
Publisher : Academic Press
ISBN 13 : 9780123708731
Total Pages : 2000 pages
Book Rating : 4.7/5 (87 download)

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Book Synopsis The Chlamydomonas Sourcebook 3-Vol set by : David Stern

Download or read book The Chlamydomonas Sourcebook 3-Vol set written by David Stern and published by Academic Press. This book was released on 2008-11-28 with total page 2000 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years Chlamydomonas reinhardtii (C. reinhardtii) has proved to be an outstanding model for investigation of signal transduction, rhodopsin-based vision, and the evolution of sexual processes. The Chlamydomonas Sourcebook, first published in 1989, summarized the development of this alga as a laboratory system beginning in the 1940s. Since the first edition published, Chlamydomonas research has grown significantly. This revised three-volume set, which includes the Sourcebook, Chlamydomonas Metabolic Processes, and Chlamydomonas Motility and Taxis Responses stands as the most comprehensive reference for this important research organism. This set retains historical material, and culture methods and illustrations from the original book, while adding molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Edited by the leading researcher in chlamydomonas science Includes best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and more Aids researchers with common laboratory problems such as contamination and details on properties of particular strains and mutants The latest advances in research including completion of the genome A broad perspective for studies in cell and molecular biology, genetics, plant physiology and related fields

The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use

Author : Elizabeth H. Harris
Publisher : Academic Press
ISBN 13 :
Total Pages : 520 pages
Book Rating : 4.8/5 (39 download)

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Book Synopsis The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use by : Elizabeth H. Harris

Download or read book The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use written by Elizabeth H. Harris and published by Academic Press. This book was released on 2009 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chlamydomonas reinhardtii (C. reinhardtii) is a unicellular green alga whose simple life cycle and ease of growth and manipulation for genetic analysis have made it a popular organism for research in diverse areas of cell biology and genetics. C. reinhardtii is important to a variety o research from studies of flagellar biogenesis and function that are highly relevant to medical research on sperm motility and ciliary function to work on chloroplast biogenesis and photosynthesis with critical importance for agriculture. In recent years it has also proved to be an outstanding model for investigation of signal transduction, rhodopsin-based vision, and the evolution of sexual processes. The Chlamydomonas Sourcebook, published in 1989 by Academic Press, summarized the development of this alga as a laboratory system beginning in the 1940s, reviewed each major research area, as well as providing methods for growth, genetic analysis, and preparation of cellular fractions. Since the first edition published in 1989, Chlamydomonas research has grown significantly. This revised three-volume set which includes the Sourcebook, Chlamydomonas Metabolic Processes, and Chlamydomonas Motility and Taxis Responses provides the most comprehensive reference for this important research organism. The reference retains historical material, culture methods and some illustrations from the original book while adding molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. * Expanded revision of gold standard reference * Includes latest advances in research including completion of the genome * Provides broad perspective with for studies in cell and molecular biology, genetics, plant physiology and related fields.

The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use

Author : Elizabeth H. Harris
Publisher : Academic Press
ISBN 13 : 9780080919553
Total Pages : 480 pages
Book Rating : 4.9/5 (195 download)

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Book Synopsis The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use by : Elizabeth H. Harris

Download or read book The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use written by Elizabeth H. Harris and published by Academic Press. This book was released on 2009-03-07 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Expanded revision of gold standard reference Includes latest advances in research, including completion of the genome Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields Available as part of a 3-Volume Set or sold individually

The Chlamydomonas Sourcebook

Author : Ursula Goodenough
Publisher : Academic Press
ISBN 13 : 0128224584
Total Pages : 462 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Ursula Goodenough

Download or read book The Chlamydomonas Sourcebook written by Ursula Goodenough and published by Academic Press. This book was released on 2023-02-15 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1) The gold-standard reference?covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use?has been fully revised and updated to include a?wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence. This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Remains the only complete reference to provide both the historical background and the most up-to-date information and applications on Chlamydomonas Includes best practices for applications in research, including methods for culture, genetic analysis, genomic and transcriptomic analysis, and mutant screening Helps researchers solve common laboratory problems, provides details on the properties of particular strains, and offers a comprehensive survey of molecular approaches Provides a broad perspective for studies in cell and molecular biology, genetics, plant physiology, and related fields

The Chlamydomonas Sourcebook 3-Vol Set

Author : Elizabeth H. Harris
Publisher : Academic Press
ISBN 13 :
Total Pages : 1128 pages
Book Rating : 4.8/5 (39 download)

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Book Synopsis The Chlamydomonas Sourcebook 3-Vol Set by : Elizabeth H. Harris

Download or read book The Chlamydomonas Sourcebook 3-Vol Set written by Elizabeth H. Harris and published by Academic Press. This book was released on 2009 with total page 1128 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years Chlamydomonas reinhardtii (C. reinhardtii) has proved to be an outstanding model for investigation of signal transduction, rhodopsin-based vision, and the evolution of sexual processes. The Chlamydomonas Sourcebook, first published in 1989, summarized the development of this alga as a laboratory system beginning in the 1940s. Since the first edition published, Chlamydomonas research has grown significantly. This revised three-volume set, which includes the Sourcebook, Chlamydomonas Metabolic Processes, and Chlamydomonas Motility and Taxis Responses stands as the most comprehensive reference for this important research organism. This set retains historical material, and culture methods and illustrations from the original book, while adding molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Edited by the leading researcher in chlamydomonas science Includes best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and more Aids researchers with common laboratory problems such as contamination and details on properties of particular strains and mutants The latest advances in research including completion of the genome A broad perspective for studies in cell and molecular biology, genetics, plant physiology and related fields

The Chlamydomonas Sourcebook

Author : Arthur Grossman
Publisher : Elsevier
ISBN 13 : 0128214309
Total Pages : 864 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Arthur Grossman

Download or read book The Chlamydomonas Sourcebook written by Arthur Grossman and published by Elsevier. This book was released on 2023-02-24 with total page 864 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Chlamydomonas Sourcebook, Third Edition, Volume Two: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community. Long known for its comprehensive insights into this highly multidisciplinary topic, this edition includes new views on Chlamydomonas branched electron transfer pathways in photosynthesis, the organization of pyrenoids and CO2 concentration mechanisms, and the fermentative-aspects of dark metabolism. Further, the book covers Chlamydomonas and its use in microalgae biotechnology. In addition to updates to previous editions, this book presents the latest in research and best practices, thus making it a must-have resource for those working with the Chlamydomonas organism. Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media Assists researchers with common laboratory problems such as contamination

The Chlamydomonas Sourcebook: Cell Motility and Behavior

Author : George Witman
Publisher : Academic Press
ISBN 13 : 9780123708762
Total Pages : 512 pages
Book Rating : 4.7/5 (87 download)

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Book Synopsis The Chlamydomonas Sourcebook: Cell Motility and Behavior by : George Witman

Download or read book The Chlamydomonas Sourcebook: Cell Motility and Behavior written by George Witman and published by Academic Press. This book was released on 2008-11-26 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport, and more will use this reference as a guide for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a Website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process Essential reference to a model species for the study of mechanisms of motility in free living cells Includes methods for Chlamydomonas motility research Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action

The Chlamydomonas Sourcebook: Organellar and Metabolic Processes

Author : David Stern
Publisher : Academic Press
ISBN 13 : 9780123708755
Total Pages : 1040 pages
Book Rating : 4.7/5 (87 download)

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Book Synopsis The Chlamydomonas Sourcebook: Organellar and Metabolic Processes by : David Stern

Download or read book The Chlamydomonas Sourcebook: Organellar and Metabolic Processes written by David Stern and published by Academic Press. This book was released on 2008-11-26 with total page 1040 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. * Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism * Includes contributions from leaders in particular areas of research * Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media * Assists researchers with common laboratory problems such as contamination * Includes valuable student demonstrations and properties of particular strains and mutants * Edited by the leading researcher in Chlamydomonas science

The Chlamydomonas Sourcebook

Author : Susan Dutcher
Publisher : Academic Press
ISBN 13 : 0128225092
Total Pages : 472 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Susan Dutcher

Download or read book The Chlamydomonas Sourcebook written by Susan Dutcher and published by Academic Press. This book was released on 2023-02-15 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Chlamydomonas Sourcebook, 3rd Edition Cell Motility and Behavior (Volume 3) The gold-standard reference?introducing this multidisciplinary science,?fully revised and updated with the latest discoveries Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the third volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Cell Motility and Behavior has been fully revised and updated to include the wealth of new resources for the Chlamydomonas community. Reflecting the significant advancement in?the understanding of the role of basal bodies and cilia play in human diseases, this volume employs quantitative proteomics and mass spectroscopy as well as?cryo?EM tomography and single particle?cryo?EM. Other topics such as current insights?on?mitosis and cytokinesis, ciliary assembly and motility, intraflagellar?transport, and more help build an understanding of human diseases of the cilium. Cell Motility and Behavior presents the latest in research and best practices, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Provides an essential reference to a model species for the study of mechanisms of motility in free living cells? Includes methods for Chlamydomonas motility research? Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process

The Chlamydomonas Sourcebook

Author : Arthur Grossman
Publisher : Academic Press
ISBN 13 : 0323910580
Total Pages : 866 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Arthur Grossman

Download or read book The Chlamydomonas Sourcebook written by Arthur Grossman and published by Academic Press. This book was released on 2023-02-15 with total page 866 pages. Available in PDF, EPUB and Kindle. Book excerpt: Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism. This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes. Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies Assists researchers with common laboratory problems such as contamination

Chlamydomonas: Biotechnology and Biomedicine

Author : Michael Hippler
Publisher : Springer
ISBN 13 : 3319663607
Total Pages : 217 pages
Book Rating : 4.3/5 (196 download)

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Book Synopsis Chlamydomonas: Biotechnology and Biomedicine by : Michael Hippler

Download or read book Chlamydomonas: Biotechnology and Biomedicine written by Michael Hippler and published by Springer. This book was released on 2017-12-30 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Advancement in Chlamydomonas biology allowed new understandings in biotechnological and biomedical related aspects.

The Chlamydomonas Sourcebook

Author : Elizabeth H. Harris
Publisher :
ISBN 13 :
Total Pages : 1071 pages
Book Rating : 4.:/5 (317 download)

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Book Synopsis The Chlamydomonas Sourcebook by : Elizabeth H. Harris

Download or read book The Chlamydomonas Sourcebook written by Elizabeth H. Harris and published by . This book was released on 2008 with total page 1071 pages. Available in PDF, EPUB and Kindle. Book excerpt:

The chlamydomonas sourcebook

Author : David B. Stern
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (298 download)

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Book Synopsis The chlamydomonas sourcebook by : David B. Stern

Download or read book The chlamydomonas sourcebook written by David B. Stern and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas

Author : J.-D. Rochaix
Publisher : Springer Science & Business Media
ISBN 13 : 0306482045
Total Pages : 733 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas by : J.-D. Rochaix

Download or read book The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas written by J.-D. Rochaix and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 733 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a thorough overview of current research with the green alga Chlamydomonas on chloroplast and mitochondrial biogenesis and function, with an emphasis on the assembly and structure-function relationships of the constituents of the photosynthetic apparatus. Contributions emphasize the multidisciplinary nature of current research in photosynthesis, combining molecular genetics, biochemical, biophysical, and physiological approaches. The 36 articles address topics including nuclear genome organization; RNA stability and processing; splicing; translation; protein targeting in the chloroplast; photosystems; pigments; glycerolipids; the ATP synthase; and ferrodoxin and thioredoxin. Further contributions address new measurements methods for photosynthetic activity in vivo; starch biosynthesis; the responses of Chlamydomonas to various stress conditions; nitrogen assimilation; and mitochondrial genetics. Annotation copyrighted by Book News, Inc., Portland, OR

Autophagy in plants and algae

Author : Diane C Bassham
Publisher : Frontiers Media SA
ISBN 13 : 2889194779
Total Pages : 103 pages
Book Rating : 4.8/5 (891 download)

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Book Synopsis Autophagy in plants and algae by : Diane C Bassham

Download or read book Autophagy in plants and algae written by Diane C Bassham and published by Frontiers Media SA. This book was released on 2015-05-15 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autophagy (also known as macroautophagy) is an evolutionarily conserved process by which cytoplasmic components are nonselectively enclosed within a double-membrane vesicle known as the autophagosome and delivered to the vacuole for degradation of toxic components and recycling of needed nutrients. This catabolic process is required for the adequate adaptation and response of the cell, and correspondingly the whole organism, to different types of stress including nutrient starvation or oxidative damage. Autophagy has been extensively investigated in yeasts and mammals but the identification of autophagy-related (ATG) genes in plant and algal genomes together with the characterization of autophagy-deficient mutants in plants have revealed that this process is structurally and functionally conserved in photosynthetic eukaryotes. Recent studies have demonstrated that autophagy is active at a basal level under normal growth in plants and is upregulated during senescence and in response to nutrient limitation, oxidative stress, salt and drought conditions and pathogen attack. Autophagy was initially considered as a non-selective pathway, but numerous observations mainly obtained in yeasts revealed that autophagy can also selectively eliminate specific proteins, protein complexes and organelles. Interestingly, several types of selective autophagy appear to be also conserved in plants, and the degradation of protein aggregates through specific adaptors or the delivery of chloroplast material to the vacuole via autophagy has been reported. This research topic aims to gather recent progress on different aspects of autophagy in plants and algae. We welcome all types of articles including original research, methods, opinions and reviews that provide new insights about the autophagy process and its regulation.