The Biology of Sciara Coprophila, and Experiments in the Control of the Striped Cucumber Beetle (Diabrotica Vittata)

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ISBN 13 :
Total Pages : 164 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis The Biology of Sciara Coprophila, and Experiments in the Control of the Striped Cucumber Beetle (Diabrotica Vittata) by : Mervyn Charles Mossop

Download or read book The Biology of Sciara Coprophila, and Experiments in the Control of the Striped Cucumber Beetle (Diabrotica Vittata) written by Mervyn Charles Mossop and published by . This book was released on 1925 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cornell University Announcements

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ISBN 13 :
Total Pages : 108 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Cornell University Announcements by : Cornell University

Download or read book Cornell University Announcements written by Cornell University and published by . This book was released on 1927 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Control of Diabrotica, Or Western Spotted Cucumber Beetle, in Deciduous Fruit Orchards

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ISBN 13 :
Total Pages : 34 pages
Book Rating : 4.:/5 (181 download)

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Book Synopsis Control of Diabrotica, Or Western Spotted Cucumber Beetle, in Deciduous Fruit Orchards by : Abe Ezra Michelbacher

Download or read book Control of Diabrotica, Or Western Spotted Cucumber Beetle, in Deciduous Fruit Orchards written by Abe Ezra Michelbacher and published by . This book was released on 1943 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Register

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ISBN 13 :
Total Pages : 266 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis Register by : Cornell University

Download or read book Register written by Cornell University and published by . This book was released on 1925 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Efficacy of Biopesticides for Organic Management of Cucumber Beetles

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ISBN 13 :
Total Pages : 146 pages
Book Rating : 4.:/5 (841 download)

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Book Synopsis Efficacy of Biopesticides for Organic Management of Cucumber Beetles by : Mary Anne Rogers

Download or read book Efficacy of Biopesticides for Organic Management of Cucumber Beetles written by Mary Anne Rogers and published by . This book was released on 2012 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organic growers are limited in crop protection techniques for cucumber beetle management. Spotted (Diabrotica undecimpunctata howardi) and striped (Acalymma vitatta) cucumber beetles are significant pests of cucurbits in the U.S. Feeding results in aesthetic damage and reduction in marketable yields as well as transmission of bacterial wilt that can result in plant mortality. Biopesticides are products formulated from naturally occurring organisms such as fungi and bacteria that are pathogenic or toxic to insect pests. Advantages to these products are that they have low environmental risk, low risk to non-target organisms including mammals and beneficial insects, and can help reduce resistance to pesticides when used in an integrated pest management program. The overall goal of this dissertation was to examine the potential of microbial products to reduce mortality and feeding by cucumber beetles for the benefit of organic producers. Chapter one is a review of the biopesticide industry, biology of microbial agents for insect pest management, the role of biopesticides in sustainable agriculture, and constraints to their use. Chapter two covers the field experiment conducted on Galia melons in 2010 and 2011 using Chromobacterium subtsugae and Beauveria bassiana. Chapter three covers the laboratory assays using Beauveria bassiana and the laboratory and field experiments using Isaria fumosorosea. Chapter four is the final experiment on the effects of these microbial agents on cucumber beetles and squash bugs in organic pumpkin production. The results indicated anti-feedant effects by Chromobacterium subtsugae and Beaveria bassiana in the laboratory assays, but field trial results were inconclusive and did not show a reduction in beetle populations or a yield increase resulting from spray applications of these microbial agents. Complications in the field studies arose from plant pathogens and physiological factors independent from cucumber beetle population and damage. Recommendations are to improve biopesticide efficacy through improving formulation and delivery, by additional screening and testing to determine efficacy on multiple life stages of the pest, and research to increase the understanding of ecological roles and interactions of microbial biopesticides in the environment.

Drosophila Cytogenetics Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592596657
Total Pages : 467 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Drosophila Cytogenetics Protocols by : Daryl S. Henderson

Download or read book Drosophila Cytogenetics Protocols written by Daryl S. Henderson and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Genomics, Proteomics, and Clinical Bacteriology

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Publisher : Springer Science & Business Media
ISBN 13 : 1592597637
Total Pages : 391 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Genomics, Proteomics, and Clinical Bacteriology by : Neil Woodford

Download or read book Genomics, Proteomics, and Clinical Bacteriology written by Neil Woodford and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gazing into crystal balls is beyond the expertise of most scientists. Yet, as we look further into the 21st century, one does not have to be Nostradamus to predict that the current genomics and proteomics "revolution" will have an immense impact on medical bacteriology. This impact is already being re- ized in many academic departments, and although encroachment on routine diagnostic bacteriology, particularly in the hospital setting, is likely to occur at a slower pace, it remains nonetheless inevitable. Therefore, it is important that no one working in bacteriology should find themselves distanced from these fundamental developments. The involvement of all clinical bacteriologists is essential if the significant achievements of genome sequencing and analysis are to be turned into tangible advances, with resulting benefits for patient care and m- agement. It is our hope that Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews will play a part in bringing such a development to fruition. The advances in genomics and proteomics have already given us frequent opportunities to reassess our knowledge and understanding of established b- terial adversaries, and have provided us with the means to identify new foes. The new knowledge gained is enabling us to reconsider, for example, our c- cepts of bacterial pathogenicity, phylogeny and novel targets for antibacterial chemotherapy. These topics, and others, are considered in Genomics, Proteomics, and Clinical Bacteriology: Methods and Reviews.

Germ Cell Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592597416
Total Pages : 421 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Germ Cell Protocols by : Heide Schatten

Download or read book Germ Cell Protocols written by Heide Schatten and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits st- ies of fertility and infertility in mammals including humans. External in vitro fertilization, now a common technique in assisted fertilization has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex that carry to- potency, an ability to develop into an entire new organism. The two volumes on germ cells combine techniques in a variety of different systems and have selected those systems that have provided landmarks in advancing our kno- edge on germ cells.

G Protein Signaling

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Publisher : Springer Science & Business Media
ISBN 13 : 1592594301
Total Pages : 246 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis G Protein Signaling by : Alan V. Smrcka

Download or read book G Protein Signaling written by Alan V. Smrcka and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alan V. Smrcka presents a collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization, and for studying the physiological roles for endogenous G proteins.

Lentivirus Gene Engineering Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592593933
Total Pages : 310 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Lentivirus Gene Engineering Protocols by : Maurizio Federico

Download or read book Lentivirus Gene Engineering Protocols written by Maurizio Federico and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.

Drosophila Protocols

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Publisher : CSHL Press
ISBN 13 : 9780879695866
Total Pages : 798 pages
Book Rating : 4.6/5 (958 download)

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Book Synopsis Drosophila Protocols by : William Sullivan

Download or read book Drosophila Protocols written by William Sullivan and published by CSHL Press. This book was released on 2000 with total page 798 pages. Available in PDF, EPUB and Kindle. Book excerpt: This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Cell Cycle Checkpoint Control Protocols

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Publisher : Humana Press
ISBN 13 : 9781588291158
Total Pages : 376 pages
Book Rating : 4.2/5 (911 download)

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Book Synopsis Cell Cycle Checkpoint Control Protocols by : Howard B. Lieberman

Download or read book Cell Cycle Checkpoint Control Protocols written by Howard B. Lieberman and published by Humana Press. This book was released on 2003-11-14 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer.

Recombinant Gene Expression

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Publisher : Springer Science & Business Media
ISBN 13 : 1592597742
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Recombinant Gene Expression by : Paulina Balbas

Download or read book Recombinant Gene Expression written by Paulina Balbas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.

Antibody Engineering

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Publisher : Springer Science & Business Media
ISBN 13 : 1592596665
Total Pages : 555 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Antibody Engineering by : Benny K. C. Lo

Download or read book Antibody Engineering written by Benny K. C. Lo and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Köhler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious—high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host’s immune s- tem for a therapeutic response. The initial excitement in finding Paul Ehrlich’s “magic bullet,” however, was met with widespread disappointment when it was demonstrated that murine antibodies frequently elicit the human anti-murine an- body (HAMA) response, thus rendering them ineffective and potentially unsafe in humans. Despite this setback, advances in recombinant DNA techniques over the last 15–20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA. The ability to p- duce bulk quantities of recombinant proteins from bacterial fermentation also fueled the design of numerous creative antibody constructs. To date, the United States Food and Drug Administration has approved more than 10 recombinant antibodies for human use, and hundreds more are in the development pipeline. The recent explosion in genomic and proteomic information appears ready to deliver many more disease targets amenable to antibody-based therapy.

Directed Enzyme Evolution

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Publisher : Springer Science & Business Media
ISBN 13 : 1592593968
Total Pages : 381 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Directed Enzyme Evolution by : Frances H. Arnold

Download or read book Directed Enzyme Evolution written by Frances H. Arnold and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.

Plant Functional Genomics

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Publisher : Springer Science & Business Media
ISBN 13 : 1592594131
Total Pages : 443 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Functional Genomics by : Erich Grotewold

Download or read book Plant Functional Genomics written by Erich Grotewold and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 443 pages. Available in PDF, EPUB and Kindle. Book excerpt: Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.

Chiral Separations

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Publisher : Springer Science & Business Media
ISBN 13 : 1592596487
Total Pages : 427 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Chiral Separations by : Gerald Gübitz

Download or read book Chiral Separations written by Gerald Gübitz and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.