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The Binding Of Escherichia Coli Rna Polymerase To Bacteriophage T7 Dna
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Book Synopsis Maximizing Gene Expression by : William Reznikoff
Download or read book Maximizing Gene Expression written by William Reznikoff and published by Elsevier. This book was released on 2014-05-20 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmids. The text then describes translation initiation, including the translation of prokaryotes and eukaryotes. The book puts emphasis on the selective degradation of abnormal proteins in bacteria. Topics include proteins rapidly hydrolyzed in E. coli; intracellular aggregates of abnormal polypeptides; energy requirement and pathway for proteins; proteolytic enzymes in E. coli; and regulation of ion expression. The text also highlights the detection of proteins produced by recombinant DNA techniques and mechanism and practice. The book is a good source of information for readers wanting to study gene expression.
Book Synopsis Ribosomes, Structure, Function, and Genetics by : Glenn Chambliss
Download or read book Ribosomes, Structure, Function, and Genetics written by Glenn Chambliss and published by . This book was released on 1980 with total page 1020 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bacteriophage T4 by : Christopher K. Mathews
Download or read book Bacteriophage T4 written by Christopher K. Mathews and published by . This book was released on 1983 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Methods for Virus Detection by : Danny L. Wiedbrauk
Download or read book Molecular Methods for Virus Detection written by Danny L. Wiedbrauk and published by Elsevier. This book was released on 1995-02-08 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables
Book Synopsis Production of Membrane Proteins by : Anne Skaja Robinson
Download or read book Production of Membrane Proteins written by Anne Skaja Robinson and published by John Wiley & Sons. This book was released on 2011-06-15 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages. Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations. Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale. Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.
Book Synopsis Enzymology Primer for Recombinant DNA Technology by : Hyone-Myong Eun
Download or read book Enzymology Primer for Recombinant DNA Technology written by Hyone-Myong Eun and published by Elsevier. This book was released on 1996-06-03 with total page 727 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. - Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology - Presents information in an easily accessible format to serve as a quick reference source - Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
Download or read book Bacteriophages written by David R. Harper and published by Springer Nature. This book was released on 2021-01-30 with total page 1376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first major reference work dedicated to the mannifold industrial and medical applications of bacteriophages provides both theoretical and practical insights into the emerging field of bacteriophage biotechnology. The book introduces to bacteriophage biology, ecology and history and reviews the latest technologies and tools in bacteriophage detection, strain optimization and nanotechnology. Usage of bacteriophages in food safety, agriculture, and different therapeutic areas is discussed in detail. This book serves as essential guide for researchers in applied microbiology, biotechnology and medicine coming from both academia and industry.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacteriophages written by Renos Savva and published by BoD – Books on Demand. This book was released on 2020-02-19 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteriophages are viruses that utilise bacterial cells as factories for their own propagation and as safe havens for their genomic material. They are capable of equipping bacteria with properties that bestow environmental advantages. They are also capable of specifically and efficiently killing bacteria.Bacteriophages are resilient in a wide diversity of environments, presumed to be as ancient as life itself, and are estimated to be the most numerous biological entities on the planet. Their overarching capacity to survive via molecular adaptation is supported by an arsenal of encoded enzymatic tools, which also enabled biotechnology. This volume includes contributions that describe bacteriophages as nanomachines, genetic engineers, and also as medicines and technologies of the future, including relevant production and process issues.
Book Synopsis RNA Polymerases as Molecular Motors by : Henri Buc
Download or read book RNA Polymerases as Molecular Motors written by Henri Buc and published by Royal Society of Chemistry. This book was released on 2009-03-10 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cell can be viewed as a 'collection of protein machines' and understanding these molecular machines requires sophisticated cooperation between cell biologists, geneticists, enzymologists, crystallographers, chemists and physicists. To observe these machines in action, researchers have developed entirely new methodologies for the detection and the nanomanipulation of single molecules. This book, written by expert scientists in the field, analyses how these diverse fields of research interact on a specific example - RNA polymerase. The book concentrates on RNA polymerases because they play a central role among all the other machines operating in the cell and are the target of a wide range of regulatory mechanisms. They have also been the subject of spectacular advances in their structural understanding in recent years, as testified by the attribution of the Nobel prize in chemistry in 2006 to Roger Kornberg. The book focuses on two aspects of the transcription cycle that have been more intensively studied thanks to this increased scientific cooperation - the recognition of the promoter by the enzyme, and the achievement of consecutive translocation steps during elongation of the RNA product. Each of these two topics is introduced by an overview, and is then presented by worldwide experts in the field, taking the viewpoint of their speciality. The overview chapters focus on the mechanism-structure interface and the structure-machine interface while the individual chapters within each section concentrate more specifically on particular processes-kinetic analysis, single-molecule spectroscopy, and termination of transcription, amongst others. Specific attention has been paid to the newcomers in the field, with careful descriptions of new emerging techniques and the constitution of an atlas of three-dimensional pictures of the enzymes involved. For more than thirty years, the study of RNA polymerases has benefited from intense cooperation between the scientific partners involved in the various fields listed above. It is hoped that a collection of essays from outstanding scientists on this subject will catalyse the convergence of scientific efforts in this field, as well as contribute to better teaching at advanced levels in Universities.
Author :Katsuhiko S. Murakami Publisher :Springer Science & Business Media ISBN 13 :3642397964 Total Pages :342 pages Book Rating :4.6/5 (423 download)
Book Synopsis Nucleic Acid Polymerases by : Katsuhiko S. Murakami
Download or read book Nucleic Acid Polymerases written by Katsuhiko S. Murakami and published by Springer Science & Business Media. This book was released on 2013-10-22 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerases of all varieties. In other words, this book is for polymerase fans by polymerase fans. Nucleic acid polymerases play a fundamental role in genome replication, maintenance, gene expression and regulation. Throughout evolution these enzymes have been pivotal in transforming life towards RNA self-replicating systems as well as into more stable DNA genomes. These enzymes are generally extremely efficient and accurate in RNA transcription and DNA replication and share common kinetic and structural features. How catalysis can be so amazingly fast without loss of specificity is a question that has intrigued researchers for over 60 years. Certain specialized polymerases that play a critical role in cellular metabolism are used for diverse biotechnological applications and are therefore an essential tool for research.
Book Synopsis Bacterial Transcriptional Control by : Irina Artsimovitch
Download or read book Bacterial Transcriptional Control written by Irina Artsimovitch and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.
Book Synopsis Control of Messenger RNA Stability by : Joel Belasco
Download or read book Control of Messenger RNA Stability written by Joel Belasco and published by Academic Press. This book was released on 1993-04-06 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first comprehensive review of mRNA stability and its implications for regulation of gene expression. Written by experts in the field, Control of Messenger RNA Stability serves both as a reference for specialists in regulation of mRNA stability and as a general introduction for a broader community of scientists. Provides perspectives from both prokaryotic and eukaryotic systems Offers a timely, comprehensive review of mRNA degradation, its regulation, and its significance in the control of gene expression Discusses the mechanisms, RNA structural determinants, and cellular factors that control mRNA degradation Evaluates experimental procedures for studying mRNA degradation
Book Synopsis Bacterial Chromatin by : Remus T. Dame
Download or read book Bacterial Chromatin written by Remus T. Dame and published by Springer Nature. This book was released on with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Life Sciences by : Robert D. Wells
Download or read book Molecular Life Sciences written by Robert D. Wells and published by Springer. This book was released on 2018-01-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Life Sciences: An Encyclopedic Reference will focus on understanding biological phenomena at the level of molecules and their interactions that govern life processes. The work will include articles on genes and genomes, protein structure and function, systems biology using genomics and proteomics as the focus, molecular aspects of cell structure and function, unifying concepts and theories from biology, chemistry, mathematics and physics that are essential for understanding the molecular life sciences (including teaching perspectives and assessment tools), and basic aspects of the various experimental approaches that are used in the Molecular Life Sciences.
Book Synopsis The Bacteriophages by : Richard Calendar
Download or read book The Bacteriophages written by Richard Calendar and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been 10 years since Plenum included a series of reviews on bacte riophages, in Comprehensive Virology. Chapters in that series contained physical-genetic maps but very little DNA sequence information. Now the complete DNA sequence is known for some phages, and the se quences for others will soon follow. During the past 10 years two phages have come into common use as reagents: A phage for cloning single copies of genes, and Ml3 for cloning and DNA sequencing by the dideoxy termi nation method. Also during that period the use of alternative sigma fac tors by RNA polymerase has become established for SPOl and T4. This seems to be a widely used mechanism in bacteria, since it has been implicated in sporulation, heat shock response, and regulation of nitro gen metabolism. The control of transcription by the binding of A phage CII protein to the -35 region of the promoter is a recent finding, and it is not known how widespread this mechanism may be. This rapid progress made me eager to solicit a new series of reviews. These contributions are of two types. Each of the first type deals with an issue that is exemplified by many kinds of phages; chapters of this type should be useful in teaching advanced courses. Chapters of the second type provide comprehensive pictures of individual phage families and should provide valuable information for use in planning experiments.
Book Synopsis Harnessing the Power of Viruses by : Boriana Marintcheva
Download or read book Harnessing the Power of Viruses written by Boriana Marintcheva and published by Academic Press. This book was released on 2017-11-13 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harnessing the Power of Viruses explores the application of scientific knowledge about viruses and their lives to solve practical challenges and further advance molecular sciences, medicine and agriculture. The book contains virus-based tools and approaches in the fields of: i) DNA manipulations in vitro and in vivo; ii) Protein expression and characterization; and iii) Virus- Host interactions as a platform for therapy and biocontrol are discussed. It steers away from traditional views of viruses and technology, focusing instead on viral molecules and molecular processes that enable science to better understand life and offer means for addressing complex biological phenomena that positively influence everyday life. The book is written at an intermediate level and is accessible to novices who are willing to acquire a basic level of understanding of key principles in molecular biology, but is also ideal for advanced readers interested in expanding their biological knowledge to include practical applications of molecular tools derived from viruses. - Explores virus-based tools and approaches in DNA manipulation, protein expression and characterization and virus-host interactions - Provides a dedicated focus on viral molecules and molecular processes that enable science to better understand life and address complex biological phenomena - Includes an overview of modern technologies in biology that were developed using viral components/elements and knowledge about viral processes