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Maximizing Gene Expression
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Book Synopsis Maximizing Gene Expression by : William Reznikoff
Download or read book Maximizing Gene Expression written by William Reznikoff and published by Elsevier. This book was released on 2014-05-20 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmids. The text then describes translation initiation, including the translation of prokaryotes and eukaryotes. The book puts emphasis on the selective degradation of abnormal proteins in bacteria. Topics include proteins rapidly hydrolyzed in E. coli; intracellular aggregates of abnormal polypeptides; energy requirement and pathway for proteins; proteolytic enzymes in E. coli; and regulation of ion expression. The text also highlights the detection of proteins produced by recombinant DNA techniques and mechanism and practice. The book is a good source of information for readers wanting to study gene expression.
Book Synopsis Heterologous Gene Expression in E.coli by : Thomas C. Evans, Jr.
Download or read book Heterologous Gene Expression in E.coli written by Thomas C. Evans, Jr. and published by Humana Press. This book was released on 2010-12-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular BiologyTM format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Book Synopsis Gene Expression During Cell Differentiation by : John Bertrand Gurdon
Download or read book Gene Expression During Cell Differentiation written by John Bertrand Gurdon and published by . This book was released on 1973 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Expression written by Michael Karin and published by . This book was released on 1993 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first in a series covering all aspects of gene expression and regulation, as well as related areas of molecular biology. It is essential reading for all molecular biologists, cell biologists, biochemists, and biotechnologists.
Book Synopsis Enhancers and Eukaryotic Gene Expression by : Yakov Gluzman
Download or read book Enhancers and Eukaryotic Gene Expression written by Yakov Gluzman and published by . This book was released on 1983 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Experimental Manipulation of Gene Expression by : Masayori Inouye
Download or read book Experimental Manipulation of Gene Expression written by Masayori Inouye and published by . This book was released on 1983 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Gene Transfer and Expression Protocols by : E. J. Murray
Download or read book Gene Transfer and Expression Protocols written by E. J. Murray and published by . This book was released on 1991-04-22 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In sequence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .
Book Synopsis Non-coding RNAs and Epigenetic Regulation of Gene Expression by : Kevin V. Morris
Download or read book Non-coding RNAs and Epigenetic Regulation of Gene Expression written by Kevin V. Morris and published by Caister Academic Press Limited. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-coding RNAs potentially play an active role in modulating gene transcription and epigenetic states. Several genes in differentiated cells may be under some form of RNA-based transcriptional and epigenetic regulatory control. This form of regulation may be controlled by selective pressures and influence the adaptability of the cell. The concept that RNA can control epigenetic states impacts our understanding of the basic fabric of the cell and may have therapeutic potential. Many studies have been carried out on the modulation of gene transcription by non-coding RNAs. This book, written by a group of distinguished scientists, represents an important overview and summary of the field to date. The 13 chapters are organized into three sections: a) Non-coding RNAs: Form, Function and Diversity; b) Non-coding RNAs: Gene Regulation and Epigenetics; and c) Non-coding RNAs: Disease and Therapeutics. This up-to-date volume is an essential book for those working in the area and represents a major information resource on current research in the fast-moving fields of epigenetics, the regulation of gene expression, and RNA research.
Book Synopsis Recombinant Gene Expression Protocols by : Rocky S. Tuan
Download or read book Recombinant Gene Expression Protocols written by Rocky S. Tuan and published by . This book was released on 1997 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expert international research workers offer the most up-to-date and comprehensive collection of techniques available for expressing one or more genes of interest. The authoritative contributors describe in depth the vectors used for expression in bacteria, yeast, viruses, nonmammalian higher eukaryotes, mammals, and plants, as well as in transgenic animals. They also provide invaluable detailed information on how to introduce the recombinant gene construct into the appropriate expression host. When combined with a companion volume by the same editor, Recombinant Proteins: Detection and Isolation Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.
Download or read book Recombinant Gene Expression written by and published by Springer. This book was released on 2011-12-14 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Gene Cloning and Expression Technologies by : Michael P. Weiner
Download or read book Gene Cloning and Expression Technologies written by Michael P. Weiner and published by Biotechniques Press. This book was released on 2002 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Control of Gene Expression in Animal Development by : John Bertrand Gurdon
Download or read book The Control of Gene Expression in Animal Development written by John Bertrand Gurdon and published by . This book was released on 1974 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :National Academies of Sciences, Engineering, and Medicine Publisher :National Academies Press ISBN 13 :0309676738 Total Pages :201 pages Book Rating :4.3/5 (96 download)
Book Synopsis Next Steps for Functional Genomics by : National Academies of Sciences, Engineering, and Medicine
Download or read book Next Steps for Functional Genomics written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2020-12-18 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the holy grails in biology is the ability to predict functional characteristics from an organism's genetic sequence. Despite decades of research since the first sequencing of an organism in 1995, scientists still do not understand exactly how the information in genes is converted into an organism's phenotype, its physical characteristics. Functional genomics attempts to make use of the vast wealth of data from "-omics" screens and projects to describe gene and protein functions and interactions. A February 2020 workshop was held to determine research needs to advance the field of functional genomics over the next 10-20 years. Speakers and participants discussed goals, strategies, and technical needs to allow functional genomics to contribute to the advancement of basic knowledge and its applications that would benefit society. This publication summarizes the presentations and discussions from the workshop.
Book Synopsis Recombinant Gene Expression by : Paulina Balbas
Download or read book Recombinant Gene Expression written by Paulina Balbas and published by Humana Press. This book was released on 2010-10-28 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Book Synopsis Nuclear Magnetic Resonance of Biological Macromolecules by :
Download or read book Nuclear Magnetic Resonance of Biological Macromolecules written by and published by Academic Press. This book was released on 2005-04-20 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Download or read book Gene Control written by David S. Latchman and published by . This book was released on 2010 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Levels of gene control -- Structure of chromatin -- Role of chromatin structure in gene control -- The process of transcription -- Transcription factors and transcriptional control -- Post-transcriptional processes -- Post-transcriptional regulation -- Gene control and cellular signaling pathways -- Gene control in embryonic development -- Control of cell-type-specific gene expression -- Gene regulation and cancer -- Gene regulation and human disease -- Conclusions and future prospects.
Book Synopsis Genes and gene regulation by : Norman MacIean
Download or read book Genes and gene regulation written by Norman MacIean and published by . This book was released on 1989 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
