Sustainable Degradation Of Lignocellulosic Biomass

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Sustainable Degradation of Lignocellulosic Biomass

Author : Anuj Chandel
Publisher : BoD – Books on Demand
ISBN 13 : 9535111191
Total Pages : 288 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Sustainable Degradation of Lignocellulosic Biomass by : Anuj Chandel

Download or read book Sustainable Degradation of Lignocellulosic Biomass written by Anuj Chandel and published by BoD – Books on Demand. This book was released on 2013-05-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.

Sustainable Degradation of Lignocellulosic Biomass

Author : Anuj Chandel
Publisher :
ISBN 13 : 9789535163398
Total Pages : 0 pages
Book Rating : 4.1/5 (633 download)

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Book Synopsis Sustainable Degradation of Lignocellulosic Biomass by : Anuj Chandel

Download or read book Sustainable Degradation of Lignocellulosic Biomass written by Anuj Chandel and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.

Encyclopedia of Alternative and Renewable Energy: Volume 12 (Sustainable Degradation of Lignocellulosic Biomass)

Author : Brad Hill
Publisher :
ISBN 13 : 9781632391865
Total Pages : 0 pages
Book Rating : 4.3/5 (918 download)

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Book Synopsis Encyclopedia of Alternative and Renewable Energy: Volume 12 (Sustainable Degradation of Lignocellulosic Biomass) by : Brad Hill

Download or read book Encyclopedia of Alternative and Renewable Energy: Volume 12 (Sustainable Degradation of Lignocellulosic Biomass) written by Brad Hill and published by . This book was released on 2015-02-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: An elucidative account based on the sustainable degradation of lignocellulosic biomass has been described in this book. It covers important aspects of sustainable degradation of lignocellulosic biomass which plays a crucial role in the economic production of numerous value-added products and biofuels which are safe to the environment. Enzymatic hydrolysis process and various pretreatment methods along with the depiction of cell wall components have been elucidated broadly. This book provides in-depth analysis regarding the enhancement in methodologies for the biomass pretreatment, the analytical procedures for biomass characterization, hemicellulose and cellulose breakdown into fermentable sugars and bioconversion of cellulosics into biofuels. Mechanistic analysis of biomass pretreatment and enzymatic hydrolysis has also been elucidated, focusing on the key factors responsible for these processes at industrial scale.

Production of Materials from Sustainable Biomass Resources

Author : Zhen Fang
Publisher : Springer
ISBN 13 : 9811337683
Total Pages : 401 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Production of Materials from Sustainable Biomass Resources by : Zhen Fang

Download or read book Production of Materials from Sustainable Biomass Resources written by Zhen Fang and published by Springer. This book was released on 2019-04-04 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a collection of studies on state-of-art techniques developed specifically for lignocellulose component derivation, and for the production of functional materials, composite polymers, carbonaceous biocatalysts, and pellets from lignocellulosic biomass, with an emphasis on using sustainable chemistry and engineering to develop innovative materials and fuels for practical application. Technological strategies for the physical processing or biological conversion of biomass for material production are also presented. All chapters were contributed by respected experts in the field from around the globe, providing a broad range of perspectives on cutting-edge applications. The book offers an ideal reference guide for academic researchers and industrial engineers in the fields of natural renewable materials, biorefinery of lignocellulose, biofuels and environmental engineering. It can also be used as a comprehensive reference source for university students in chemical engineering, material science and environmental engineering.

Biomass Now

Author : Miodrag Darko Matovic
Publisher : BoD – Books on Demand
ISBN 13 : 9535111051
Total Pages : 556 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Biomass Now by : Miodrag Darko Matovic

Download or read book Biomass Now written by Miodrag Darko Matovic and published by BoD – Books on Demand. This book was released on 2013-04-30 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: This two-volume book on biomass is a reflection of the increase in biomass related research and applications, driven by overall higher interest in sustainable energy and food sources, by increased awareness of potentials and pitfalls of using biomass for energy, by the concerns for food supply and by multitude of potential biomass uses as a source material in organic chemistry, bringing in the concept of bio-refinery. It reflects the trend in broadening of biomass related research and an increased focus on second-generation bio-fuels. Its total of 40 chapters spans over diverse areas of biomass research, grouped into 9 themes.

Lignocellulose for Future Bioeconomy

Author : Hidayah Ariffin
Publisher : Elsevier
ISBN 13 : 0128166053
Total Pages : 360 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Lignocellulose for Future Bioeconomy by : Hidayah Ariffin

Download or read book Lignocellulose for Future Bioeconomy written by Hidayah Ariffin and published by Elsevier. This book was released on 2019-04-20 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lignocellulose for Future Bioeconomy discusses the conversion and utilization of lignocellulosic biomass. This book focuses on the utilization of lignocelluloses for various products, including biopolymers, bionanomaterials and bioproducts. Recent findings in scientific investigation, engineering, product development, economic and lifecycle analysis are discussed, as are current synthesis technologies and potential applications. The book progresses from a discussion of the potential sources of biomass, to the refinement and processing of materials. A sampling of various sustainability issues faced by industries in their production methods and a look at real world examples of the use of lignocellulose-based materials in the bioeconomy round out the discussion. Presents information on lignocellulosic biomass management and its utilization for the production of bioproducts, biopolymers and bionanomaterials Highlights the applications of advanced materials developed from lignocellulosic biomass and their contribution towards future bioeconomy Discusses the lifecycle of lignocellulosic biomass

Sustainable Bioenergy

Author : Mahendra Rai
Publisher : Elsevier
ISBN 13 : 9780128176542
Total Pages : 0 pages
Book Rating : 4.1/5 (765 download)

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Book Synopsis Sustainable Bioenergy by : Mahendra Rai

Download or read book Sustainable Bioenergy written by Mahendra Rai and published by Elsevier. This book was released on 2019-05-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sustainable Bioenergy: Advances and Impacts presents a careful overview of advances and promising innovation in the development of various bioenergy technologies. It covers the production of bio-jet fuel, algal biofuels, recent developments in bioprocesses, nanotechnology applications for energy conversion, the role of different catalysts in the production of biofuels, and the impacts of those fuels on society. The book brings together global experts to form a big picture of cutting-edge research in sustainable bioenergy and biofuels. It is an ideal resource for researchers, students, energy analysts and policymakers who will benefit from the book's overview of impacts and innovative needs.

Microbial Resource Technologies for Sustainable Development

Author : Joginder Singh
Publisher : Elsevier
ISBN 13 : 0323913261
Total Pages : 492 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Microbial Resource Technologies for Sustainable Development by : Joginder Singh

Download or read book Microbial Resource Technologies for Sustainable Development written by Joginder Singh and published by Elsevier. This book was released on 2022-06-25 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Resource Technologies for Sustainable Development describes the production and uses of microbial cells and metabolites and reviews the microbial resource technologies associated with providing sustainable solutions options in future endeavors in managing microbial resources. The book includes the recent development and scientific demonstrations of microbial technologies in the relationship between microbes and the environment, focusing on its effective resource management to achieve agricultural and environmental sustainability. Topics covered in the book include recent applications and exploration of the development of Marine Microbial Technologies for marine resources, soil microbes as biopolymers for enhancing mechanical properties of soil, and more. Other topics discussed include rhizosphere microbiome for enhancement of the cereal crops, endophytic fungal communities in crops grown under different farming systems, microbiota of termite for lignocellulose breakdown, microbial consortium technologies to produce biomethane from waste effluents, microbial technologies for sustainable food additives production, biological synthesis of the nanoparticles, fungal cellulases, and efficient biofuel and acetic acid production using waste residues with an emphasis on the commercial exploitation of such microbial technologies. Discusses the enhancement of plant production through growth-promoting microbes Considers microbial degradation and environmental management of wastes Covers microbial applications in biofuel and bioenergy production Explores plant-microbe interactions for removal of heavy metals from contaminated areas Explains engineered microorganisms for effective bioremediation Describes potential indigenous/effective microbes for food and industrial treatment processes Presents research on microbes for sustainable agricultural practices

Valorization of Biomass Wastes for Environmental Sustainability

Author : Arun Lal Srivastav
Publisher : Springer Nature
ISBN 13 : 3031524853
Total Pages : 335 pages
Book Rating : 4.0/5 (315 download)

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Book Synopsis Valorization of Biomass Wastes for Environmental Sustainability by : Arun Lal Srivastav

Download or read book Valorization of Biomass Wastes for Environmental Sustainability written by Arun Lal Srivastav and published by Springer Nature. This book was released on 2024 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zusammenfassung: This volume discusses the reduction, recycling, and reuse of industrial and agricultural biomass wastes to develop value-added products using environmentally sustainable practices and technologies. Through these waste valorization approaches, biomass waste materials can be converted into useful bio-chemical products, sustainable construction materials, polymers, bio-energy, and bio-fuel as sustainable alternatives to products and materials with negative environmental and health consequences. The chapters highlight the development and implementation of eco-friendly solutions to biomass waste production with the aim of reducing natural resource deterioration, bolstering rural and small-scale business systems in communities impacted by pollution and climate change, and providing power from residual biomass to broadly reduce environmental impacts through improved waste management practices. The book is intended to be a useful resource for researchers, policymakers, NGOs, government agencies, and local community authorities working in waste management and environmental sustainability

Biohydrogen Production: Sustainability of Current Technology and Future Perspective

Author : Anoop Singh
Publisher : Springer
ISBN 13 : 8132235770
Total Pages : 320 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Biohydrogen Production: Sustainability of Current Technology and Future Perspective by : Anoop Singh

Download or read book Biohydrogen Production: Sustainability of Current Technology and Future Perspective written by Anoop Singh and published by Springer. This book was released on 2016-08-22 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Increase in green, renewable and sustainable energy demand due to higher environmental impacts (e.g. Greenhouse gases emissions, climate change, etc.) on consumption of fossil fuel resource put down an extra pressure on government, researchers and industrialists. Among several available biofuel options, biohydrogen is considered as one of the best environmentally clean fuel and a strong candidate to fulfil the future demand of sustainable energy resource. Although, biohydrogen production technology and its use as a fuel is still in infancy stage. Selection of most sustainable production pathway, increase in production upto industrial scale and cost efficiency are some issue still persist with the biohydrogen research. “Biohydrogen Production: Sustainability of Current Technology and Future Perspective” is giving an insight for the sustainable production of biohydrogen at industrial scale. The process of biohydrogen production is complex and to opt the best suited production system for industrial scale is a frantic task. This book will provide an in depth information on all available technologies for biohydrogen production and feedstock options to choose upon. This book is also providing information on present status of the research in the field and possibility to change future fuel economy in to biohydrogen economy. Experts views provided in the chapters by renowned researchers from all over the globe in the field of biohydrogen research made this book a cornucopia of present research and future perspective of biohydrogen. This book is targeted at the researchers working on biohydrogen as well as the bioenergy scientist planning to move towards biohydrogen research. This book will provide a platform for motivation of researchers and industrialists for innovative ideas and thoughts to bring biohydrogen production at industrial scale.

Biotechnology for Zero Waste

Author : Chaudhery Mustansar Hussain
Publisher : John Wiley & Sons
ISBN 13 : 3527348980
Total Pages : 628 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Biotechnology for Zero Waste by : Chaudhery Mustansar Hussain

Download or read book Biotechnology for Zero Waste written by Chaudhery Mustansar Hussain and published by John Wiley & Sons. This book was released on 2022-01-18 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology for Zero Waste The use of biotechnology to minimize waste and maximize resource valorization In Biotechnology for Zero Waste: Emerging Waste Management Techniques, accomplished environmental researchers Drs. Chaudhery Mustansar Hussain and Ravi Kumar Kadeppagari deliver a robust exploration of the role of biotechnology in reducing waste and creating a zero-waste environment. The editors provide resources covering perspectives in waste management like anaerobic co-digestion, integrated biosystems, immobilized enzymes, zero waste biorefineries, microbial fuel cell technology, membrane bioreactors, nano biomaterials, and more. Ideal for sustainability professionals, this book comprehensively sums up the state-of-the-art biotechnologies powering the latest advances in zero-waste strategies. The renowned contributors address topics like bioconversion and biotransformation and detail the concept of the circular economy. Biotechnology for Zero Waste effectively guides readers on the path to creating sustainable products from waste. The book also includes: A thorough introduction to modern perspectives on zero waste drives, including anaerobic co-digestion as a smart approach for enhancing biogas production Comprehensive explorations of bioremediation for zero waste, biological degradation systems, and bioleaching and biosorption of waste Practical discussions of bioreactors for zero waste and waste2energy with biotechnology An in-depth examination of emerging technologies, including nanobiotechnology for zero waste and the economics and commercialization of zero waste biotechnologies Perfect for process engineers, natural products, environmental, soil, and inorganic chemists, Biotechnology for Zero Waste: Emerging Waste Management Techniques will also earn a place in the libraries of food technologists, biotechnologists, agricultural scientists, and microbiologists.

Lignocellulosic Biomass in Biotechnology

Author : Pratima Bajpai
Publisher : Elsevier
ISBN 13 : 0128219068
Total Pages : 248 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Lignocellulosic Biomass in Biotechnology by : Pratima Bajpai

Download or read book Lignocellulosic Biomass in Biotechnology written by Pratima Bajpai and published by Elsevier. This book was released on 2021-07-13 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lignocellulosic Biomass in Biotechnology highlights significant aspects of lignocellulose biotechnology, demonstrating its potential value from an application perspective. Sections cover the physico-chemical characteristics of lignocellulosic biomass, the physical and structural properties of hemicelluloses, celluloses and lignin, sources of lignocellulosic biomass , microorganisms and their lignocellulytic enzymes, enzymatic degradation of lignocellulosic biomass, regulation of cell-wall degrading enzymes, barriers to lignocellulose biodegradation, biotechnological importance of lignocellulosic biomass, lignocellulosic pretreatment techniques, bioprocessing of lignocellulosic biomass, lignocellulosic biomass pretreatment methods, valuable chemicals and products, techno-economic evaluation and future perspectives. This book answers questions surrounding the biotechnology of lignocelluloses. It is ideal for both students and professionals in the industry supply chain. It also provides a reference for researchers and administrators engaged in the utilization and industrial development of agricultural resources. Presents recent advances in the processing of lignocellulosic biomass Highlights significant aspects of lignocelluloses biotechnology, with an emphasis on its potential value from an application perspective Looks at the cost of enzymes and the potential of modern approaches that could be employed to reduce the cost Summarizes the new achievements that have emerged in the biotechnology of lignocelluloses in recent years Discusses a wide range of topics related to the fundamental and applied aspects of lignocellulose utilization, processing and biotechnological applications

Research Approaches to Sustainable Biomass Systems

Author : Seishu Tojo
Publisher : Academic Press
ISBN 13 : 0124046835
Total Pages : 418 pages
Book Rating : 4.1/5 (24 download)

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Book Synopsis Research Approaches to Sustainable Biomass Systems by : Seishu Tojo

Download or read book Research Approaches to Sustainable Biomass Systems written by Seishu Tojo and published by Academic Press. This book was released on 2013-09-02 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Researchers, students and engineers working with biofuels and biomass are increasingly expected to comprehend a new holistic systems perspective that begins with wise crop breeding and cultivation techniques and informs the entire conversion to energy process. This volume provides diverse examples of successfully implemented sustainable biomass research in Asia, highlighting the challenges faced by designers of new biomass production facilities and tips on how to develop approaches to overcome them. In addition to providing an authoritative guide on the utilization of the authors’ sample feedstocks, rice straw and sunflower, the authors provide lessons relevant to stakeholders involved with all manner of biomass production projects by drawing out important comparisons and contrasts that must be taken into account when deciding how to utilize biomass as an energy resource in a way that is economically feasible and environmentally sustainable. Provides diverse examples of successfully implemented biomass research, highlighting insights on common bottlenecks and approaches developed to overcome them Features coverage of the full feedstock life cycle, from crop breeding to commercial application, focusing on 3 key areas: biomass production, biofuel conversion technologies, and sustainable practices Examines two regionally compatible feedstock, rice-straw and sunflower, performing a compare and contrast analysis of agricultural production methods, economics, conversion systems, and environmental impacts

Lignocellulose Bioconversion Through White Biotechnology

Author : Anuj Kumar Chandel
Publisher : John Wiley & Sons
ISBN 13 : 1119735955
Total Pages : 436 pages
Book Rating : 4.1/5 (197 download)

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Book Synopsis Lignocellulose Bioconversion Through White Biotechnology by : Anuj Kumar Chandel

Download or read book Lignocellulose Bioconversion Through White Biotechnology written by Anuj Kumar Chandel and published by John Wiley & Sons. This book was released on 2022-09-13 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lignocellulose Bioconversion Through White Biotechnology Comprehensive resource summarizing the recent technological advancements in white biotechnology and biomass conversion into fuels, chemicals, food, and more Lignocellulose Bioconversion Through White Biotechnology presents cutting-edge information on lignocellulose biomass conversion, detailing how white biotechnology can develop sustainable biomass pretreatment methods, effective plant cell wall degrading enzymes to yield high quality cellulosic sugars, and the eventual conversion of these sugars into fuels, chemicals, and other materials. To provide comprehensive coverage of the subject, the work offers in-depth critical analysis into both techno-economic and life cycle analysis of lignocellulose-based products. Each of the 16 chapters, written by a well-qualified and established researchers, academics, or engineers, presents key information on a specific facet of lignocellulose-based products. Topics covered include: Lignocellulose feedstock availability, types of feedstock, and potential crops that are of high interest to the industry Lignocellulose bioconversion, including both foundational technical aspects and new modern developments Plant cell wall degrading enzymes, including cellulase improvement and production challenges/solutions when scaling up Improvements and challenges when considering fermenting microorganisms for cellulosic sugars utilization Scaling up of lignocellulose conversion, including insight into current challenges and future practices Techno-economic aspects of lignocellulose feedstock conversion, green consumerism and industrialization aspects of renewable fuels/chemicals Students, academics, researchers, bio-business analysts, and policy-makers working on sustainable fuels, chemicals, materials, and renewable fuels can use Lignocellulose Bioconversion Through White Biotechnology to gain invaluable expert insight into the subject, its current state of the art, and potential exciting future avenues to explore.

Biomass, Biofuels, Biochemicals

Author : Hu Li
Publisher : Elsevier
ISBN 13 : 032386001X
Total Pages : 764 pages
Book Rating : 4.3/5 (238 download)

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Book Synopsis Biomass, Biofuels, Biochemicals by : Hu Li

Download or read book Biomass, Biofuels, Biochemicals written by Hu Li and published by Elsevier. This book was released on 2022-01-30 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemicals and Materials Production from Sustainable Biomass Resources provides a detailed overview of the experimentally developed approaches and strategies that facilitate carbon-based materials and fine chemicals derivation from biomass feedstocks with robust catalyst systems and renewed conversion routes. In addition, the book highlights theoretical methods like techno-economic analysis of biobutanol synthesis. As academia and industry are now striving to substitute fossil-based chemicals with alternative renewable resources, second-generation lignocellulosic biomass which does not depend on the food cycle has become increasingly important. Lignocellulosic biomass is composed of three major polymeric components - lignin, cellulose and hemicellulose. The polymers can be degraded into monomeric counterparts through selective conversion routes like hydrolysis of cellulose to glucose and of hemicellulose to xylose. Includes the recent development of biomass-derived high-value chemicals and functional materials Describes theoretical and technical details of specific conversion routes and preparation methods Covers jointly organic transformations, catalytic synthesis, reaction mechanisms, thermal stability, reaction parameters and solvent effects

Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass

Author : Arindam Kuila
Publisher : Elsevier
ISBN 13 : 0128179546
Total Pages : 256 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass by : Arindam Kuila

Download or read book Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass written by Arindam Kuila and published by Elsevier. This book was released on 2020-02-19 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass

Lignocellulose Biodegradation

Author : American Chemical Society. Meeting
Publisher :
ISBN 13 :
Total Pages : 424 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Lignocellulose Biodegradation by : American Chemical Society. Meeting

Download or read book Lignocellulose Biodegradation written by American Chemical Society. Meeting and published by . This book was released on 2004 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lignocellulose Biodegradation will be useful for chemists, biochemists, microbiologists, molecular biologists, and biochemical engineers. This book describes advances in lignocellulose biodegradation and application in biotechnology. It contains a combination of original research and review chapters. An overview chapter on lignocellulose biodegradation and applications in biotechnology focuses on recent research progress in the field. Lignocellulose Biodegradation includes sections on pretreatment, biodegradation, enzyme characterization and application.