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Stategies To Improve Pancreatic Islet Isolation And Transplantation
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Book Synopsis Pancreatic Islet Isolation by : Miriam Ramírez-Domínguez
Download or read book Pancreatic Islet Isolation written by Miriam Ramírez-Domínguez and published by Springer. This book was released on 2016-09-01 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate “work of a craftsman”. This book, which is aimed at beginners and experts alike, is a survey of the current state-of–the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevant species (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.
Book Synopsis Pancreas and Islet Transplantation by : Nadey S. Hakim
Download or read book Pancreas and Islet Transplantation written by Nadey S. Hakim and published by . This book was released on 2002 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diabetes Mellitus is the principle cause of kidney failure and blindness in adults and leads to more cases of amputation and impotence than any other disease. It is one of the most common chronic diseases in childhood. The aims of pancreas or islet transplantation are to improve the quality of life of patients with insulin dependent diabetes mellitus and to ameliorate secondary complications. This book provides a comprehensive and international review of the recent advances in pancreas and islet transplantation. It covers surgical techniques, issues surrounding organ preservation, immunosuppression and the control of other complications, all of which contribute to the potential for such transplantations to evolve as the treatment of choice for insulin dependent diabetes. The editors have compiled a strong and international team of contribution authors. This book is essential reading for transplant surgeons and all those involved in researching or treating diabetes mellitus.
Book Synopsis Stategies to Improve Pancreatic Islet Isolation and Transplantation by : Melissa Lynn Brown
Download or read book Stategies to Improve Pancreatic Islet Isolation and Transplantation written by Melissa Lynn Brown and published by . This book was released on 2004 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Islets of Langerhans, 2. Ed by : Shahidul Islam
Download or read book Islets of Langerhans, 2. Ed written by Shahidul Islam and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Pancreatic Islet Cell Transplantation by : Camillo Ricordi
Download or read book Pancreatic Islet Cell Transplantation written by Camillo Ricordi and published by Landes Bioscience. This book was released on 1992 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intended for researchers and clinicians who work with cell transplantation, this text discusses the latest methods and gives an overall update of the major fields of islet transplantation. Included are reports of recent clinical trials on successful islet transplantation in humans.
Book Synopsis The Endocrine Pancreas by : Ellis Samols
Download or read book The Endocrine Pancreas written by Ellis Samols and published by Lippincott Williams & Wilkins. This book was released on 1991 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Inflammatory Changes in Human Pancreatic Islet Cells by : Andrew M. Jackson
Download or read book Analysis of Inflammatory Changes in Human Pancreatic Islet Cells written by Andrew M. Jackson and published by . This book was released on 2009 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pancreatic islet cell transplantation is a promising investigational research treatment for labile type 1 diabetes mellitus. However, several obstacles still exist to the implementation of clinical islet cell transplantation as a standard therapy. These obstacles include a shortage of donor pancreata, an imperfect islet isolation procedure, significant loss of islets during the peri-transplant period, and islet toxicity of immunosuppressive drugs. The present study is focused on addressing two of these major hurdles; namely, identification of factors affecting the islet isolation, and understanding the mechanism involved in the peri-transplant loss of islets. Initial analysis was centered on the effect of cold ischemia time (CIT) on the islet isolation outcome. Comparison of varying CIT and several factors that determine the quantity and quality of islets obtained from 52 isolations was performed. This analysis showed that CIT of less than four hours significantly improved islet isolation results. This finding could help improve the current strategy used in clinical islet transplantation. We hypothesized that following transplantation, the inflammatory environment will specifically alter gene expression in transplanted islets, and also will induce surface expression of HLA-class II molecules. This, in turn, could cause anti-donor response resulting in islet destruction. To test the above hypotheses, two studies were performed. Islet cells were treated with control or type 1 diabetic serum. Gene expression was then analyzed using micro array and confirmed by real-time PCR. Islets treated with diabetic serum demonstrated specific induction of multiple genes reported to have secondary roles in angiogenesis while inhibiting transcription of genes with protective attributes against environmental stresses. Islet cells were treated with IFN[gamma] and TNF[alpha] and analyzed for HLA class II induction by real-time PCR analysis, flow cytometry, and immunofluorescent imaging. Cytokine treated islets demonstrated significantly upregulated HLA class II gene transcription and surface expression. Importantly, islet transplant recipient serum showed increased binding and cytotoxicity specifically directed against cytokine treated islets. Together these data suggest that in the context of hepatic portal vein transplantation, islet cells contribute to the innate and adaptive immune response during the peri-transplant period resulting in islet destruction.
Book Synopsis Immunoendocrinology: Scientific and Clinical Aspects by : George S. Eisenbarth
Download or read book Immunoendocrinology: Scientific and Clinical Aspects written by George S. Eisenbarth and published by Springer Science & Business Media. This book was released on 2010-12-13 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoendocrinology is a rapidly developing field of research that seeks to understand the intersection of the immune and endocrine systems. Immunoendocrinology: Scientific and Clinical Aspects explores in detail the current knowledge of immunoendocrinology, namely endocrine disorders produced by disorders of immune function. Chapters cover both basic pathophysiology informed by studies of animal models as well as current understanding of multiple related clinical diseases—their pathophysiology, diagnosis, and therapy. Immunoendocrinology: Scientific and Clinical Aspects captures the central role of immunoendocrinologic processes in the pathogenesis of not only type 1 diabetes but in a range of other autoimmune and endocrine disorders.
Book Synopsis Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas by : Giuseppe Orlando
Download or read book Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas written by Giuseppe Orlando and published by Academic Press. This book was released on 2019-11-09 with total page 890 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas, Volume 1, sets a new standard in transplant and regenerative medicine. The book details the-state-of-the-art in modern whole pancreas and islet transplantation, including donor selection, immunosuppression, complications, allograft pathology, and more. As regenerative medicine is changing the premise of solid organ transplantation, this volume catalogs the technologies being developed and the methods being implemented to bioengineer or regenerate the endocrine pancreas in order to more effectively treat diabetes. Edited and authored by unparalleled leaders in the field, this new volume argues for a much needed synergy between organ transplantation and regenerative medicine. Provides comprehensive and cutting-edge knowledge of whole pancreas and islet transplantation Includes sections that address donor selection, immunosuppression, complications, allograft pathology, and more Offers an update on the progress of regenerative medicine research aimed at beta cells replacement in the treatment of diabetes
Book Synopsis Cellular Transplantation by : Craig Halberstadt
Download or read book Cellular Transplantation written by Craig Halberstadt and published by Elsevier. This book was released on 2011-10-10 with total page 699 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been tremendous strides in cellular transplantation in recent years, leading to accepted practice for the treatment of certain diseases, and use for many others in trial phases. The long history of cellular transplantation, or the transfer of cells from one organism or region of the body to another, has been revolutionized by advances in stem cell research, as well as developments in gene therapy. Cellular Transplants: From Lab to Clinic provides a thorough foundation of the basic science underpinning this exciting field, expert overviews of the state-of-the-art, and detailed description of clinical success stories to date, as well as insights into the road ahead. As highlighted by this timely and authoritative survey, scale-up technologies and whole organ transplantation are among the hurdles representing the next frontier. The contents are organized into four main sections, with the first covering basic biology, including transplant immunology, the use of immunosuppressive drugs, stem cell biology, and the development of donor animals for transplantation. The next part looks at peripheral and reconstructive applications, followed by a section devoted to transplantation for diseases of the central nervous system. The last part presents efforts to address the key challenges ahead, such as identifying novel transplantable cells and integrating biomaterials and nanotechnology with cell matrices. Provides detailed description of clinical trials in cell transplantation Review of current therapeutic approaches Coverage of the broad range of diseases addressed by cell therapeutics Discussion of stem cell biology and its role in transplantation
Book Synopsis Organ Donation and Transplantation by : Georgios Tsoulfas
Download or read book Organ Donation and Transplantation written by Georgios Tsoulfas and published by BoD – Books on Demand. This book was released on 2018-07-25 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the most interesting and at the same time most challenging fields of medicine and surgery has been that of organ donation and transplantation. It is a field that has made tremendous strides during the last few decades through the combined input and efforts of scientists from various specialties. What started as a dream of pioneers has become a reality for the thousands of our patients whose lives can now be saved and improved. However, at the same time, the challenges remain significant and so do the expectations. This book will be a collection of chapters describing these same challenges involved including the ethical, legal, and medical issues in organ donation and the technical and immunological problems the experts are facing involved in the care of these patients.The authors of this book represent a team of true global experts on the topic. In addition to the knowledge shared, the authors provide their personal clinical experience on a variety of different aspects of organ donation and transplantation.
Book Synopsis Strategies to Improve Outcome After Pancreas and Islet Transplantation by :
Download or read book Strategies to Improve Outcome After Pancreas and Islet Transplantation written by and published by . This book was released on 2022 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Pancreatic Beta Cell written by and published by Academic Press. This book was released on 2014-02-20 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1943, Vitamins and Hormones is the longest-running serial published by Academic Press. The Series provides up-to-date information on vitamin and hormone research spanning data from molecular biology to the clinic. A volume can focus on a single molecule or on a disease that is related to vitamins or hormones. A hormone is interpreted broadly so that related substances, such as transmitters, cytokines, growth factors and others can be reviewed. This volume focuses on the pancreatic beta cell. Expertise of the contributors Coverage of a vast array of subjects In depth current information at the molecular to the clinical levels Three-dimensional structures in color Elaborate signaling pathways
Book Synopsis Strategies to Improve the Survival and Functional of Isolated Pancreatic Islet Cells by : Erika Madeleine Roshanravan-Krähenbühl
Download or read book Strategies to Improve the Survival and Functional of Isolated Pancreatic Islet Cells written by Erika Madeleine Roshanravan-Krähenbühl and published by . This book was released on 2007 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Strategies to Improve the Survival and Function of Isolated Pancreatic Islet Cells by : Erika Madeleine Roshanravan-Krähenbühl
Download or read book Strategies to Improve the Survival and Function of Isolated Pancreatic Islet Cells written by Erika Madeleine Roshanravan-Krähenbühl and published by . This book was released on 2007 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Optimal Delivery of Therapeutic Genes to Pancreatic Islets by : Amy Hughes
Download or read book Optimal Delivery of Therapeutic Genes to Pancreatic Islets written by Amy Hughes and published by . This book was released on 2012 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Islet transplantation is a promising therapeutic option for Type 1 Diabetic (T1D) patients, with the ability to improve glycometabolic control and in select cases achieve insulin independence. Intraportally transplanted islets must reside in the hostile environment of the liver, where they are exposed to the instant blood mediated inflammatory reaction (IBMIR), alloimmunity, recurrence of islet specific autoimmunity, a highly toxic pro-inflammatory cytokine storm (e.g. IL-1[beta], IFN-[alpha], IFN-[gamma] and TNF-[alpha]) and hypoxia due to inadequate revascularization post-transplantation. The early loss of functional islet mass (50-70%) due to apoptosis following clinical transplantation contributes to islet allograft failure. Strategies to prevent apoptosis are therefore highly desirable to enhance islet survival for transplantation. In Chapter 3, the ability of Adenoviral (Ad) and Adeno-Associated Viral (AAV)-based vectors expressing a green fluorescent protein (GFP) reporter gene to transduce isolated human and rat pancreatic islets was investigated. Specific interest was placed on tyrosine mutant AAV-based vector types, which have not been previously explored in human and rodent pancreatic islets. Ad efficiently transduced isolated human and rat pancreatic islets while AAV failed to transduce human islets and showed a varied ability to transduce rat islets. The results in this chapter demonstrate that Ad vectors are more efficient at transducing isolated islets than AAV-based vector types. Chapter 4 aimed to characterise an Ad-based vector encoding an anti-apoptotic molecule termed Insulin-like Growth Factor-II (Ad-IGF-II). Ad-IGF-II effectively transduced rat pancreatic islets without affecting islet viability or function and did not induce uncontrolled islet cell proliferation. The results in this chapter suggest that Ad-IGF-II is an effective and non-toxic vector type for use in an islet gene therapy setting. In Chapter 5 and Chapter 6, the influence of local human IGF-II over expression on rat pancreatic islet cell survival in vitro and in vivo was examined, respectively. Over expression of IGF-II in islets resulted in enhanced islet survival in vitro and in an in vivo marginal mass islet transplant model. Transplantation of IGF-II over expressing islets under the kidney capsule of diabetic NOD-SCID mice restored euglycemia in 78% of recipients, compared to 46% and 18% of untransduced and Ad-GFP transduced control islet recipients, respectively. In summary, this thesis demonstrated that compared to AAV, Ad is currently the optimal vector for use in an islet gene therapy setting. Moreover, over expression of IGF-II did not affect the viability or insulin secreting capacity of islets. Finally, the induced expression of anti-apoptotic IGF-II led to enhanced islet survival in vitro and improved transplant outcomes in an in vivo marginal mass islet transplant model, indicating that IGF-II gene transfer is a potentially powerful tool to improve islet survival post-transplantation.
Book Synopsis Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas by : Giuseppe Orlando
Download or read book Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas written by Giuseppe Orlando and published by Academic Press. This book was released on 2019-11-10 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas, Volume 2, sets a new standard in transplant and regenerative medicine. The book details the state-of-the-art in modern islet auto-transplantation, also discussing current progress in regenerative medicine research in diabetes medicine. Regenerative medicine is changing the premise of solid organ transplantation, hence this volume catalogs technologies being developed and methods being implemented. Bioengineering and regenerating beta cells, clinical pancreas and islet transplantation, tissue engineering, biomaterial sciences, stem cell biology and developmental biology are all addressed and applied directly to diabetes medicine. Provides comprehensive and cutting-edge knowledge of whole pancreas and islet transplantation Addresses imaging, treatment, scaffold technology, the use of stem cells to generate insulin, 3D printing, and more Offers an update on the progress of regenerative medicine research aimed at beta cell replacement for the treatment of diabetes
