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Rna Interference In Practice
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Book Synopsis RNA Interference in Practice by : Ute Schepers
Download or read book RNA Interference in Practice written by Ute Schepers and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
Download or read book RNA Interference written by Dr. Tim Doran and published by CABI. This book was released on 2009 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.
Download or read book RNA Interference (RNAi) written by Wiley and published by John Wiley & Sons. This book was released on 2013-11-26 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 2006 Nobel Prize in Physiology or Medicine was awarded toAndrew Z. Fire and Craig C. Mello for their discovery of RNAinterference (RNAi). RNAi is a biological process in whichRNA molecules can silence (inhibit) or up- or down-regulategene expression, typically by causing the destruction of specificmessenger RNA (mRNA) molecules. This collection of articlesbegins with an introduction to RNAi, and proceeds to describe usesof this technology in various approaches to disease treatment,including gene therapy. Several laboratory protocols for silencinggenes via RNAi are also provided, as are protocols fordown-regulating and then rescuing those down-regulated genes, whichdemonstrates specificity of the approach. This e-book — a curated collection from eLS, WIREs, andCurrent Protocols — offers a fantastic introduction to thefield of RNA interference for students or interdisciplinarycollaborators. Table of Contents: Introduction Overview of Gene Silencing by RNA Interference Current Protocols in Nucleic Acid Chemistry Daniel H. Kim, John J. Rossi Biogenesis and function of endogenous and exogenous siRNAs WIREs RNA Nicholas M. Snead, John J. Rossi Techniques & Applications Small Silencing RNAs and Gene Therapy eLS Dirk Grimm RNAi Screening: New Approaches, Understandings, andOrganisms WIREs RNA Stephanie E. Mohr, Norbert Perrimon Delivery of RNAi mediators WIREs RNA Lance P. Ford, Masoud M. Toloue Protocols Silencing of Gene Expression in Cultured Cells Using SmallInterfering RNAs Current Protocols in Cell Biology Kumi Sakurai, Pritsana Chomchan, John J. Rossi Gene Down-Regulation with Short Hairpin RNAs and Validation ofSpecificity by Inducible Rescue in Mammalian Cells Current Protocols in Cell Biology Hoi Tang Ma, Randy Y.C. Poon
Book Synopsis RNA Interference from Biology to Therapeutics by : Kenneth A. Howard
Download or read book RNA Interference from Biology to Therapeutics written by Kenneth A. Howard and published by Springer Science & Business Media. This book was released on 2012-10-19 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: The enormous potential of siRNA as a therapeutic has led to an explosion of interest from the scientific community. There has been intense interest from Big Pharma to capitalise on this new technology but the fact remains that delivery is a key determinant in realizing the full clinical potential of RNA interference. There is an urgent need for better delivery methods to take this technology forward. This book addresses the role of different RNAi molecules in cellular processes as rational for diagnostic and therapeutic approaches. This book will cover RNAi therapeutic design to optimize siRNA potency and reduce off-target effects and current delivery technologies to overcome both intracellular and extracellular barriers. The reader will gain an insight into RNA interference from the cellular mechanisms to screening to siRNA design right through to diagnostic and therapeutic applications.
Download or read book RNAi Technology written by R. K. Gaur and published by CRC Press. This book was released on 2016-04-19 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Book Synopsis RNA Interference by : David R. Engelke
Download or read book RNA Interference written by David R. Engelke and published by Elsevier. This book was released on 2005-02-28 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Book Synopsis Cellular RNA Interference Mechanisms by :
Download or read book Cellular RNA Interference Mechanisms written by and published by Academic Press. This book was released on 2011-08-12 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores some of the most exciting recent advances in basic research on cellular RNA interference mechanisms and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and ideas Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields
Book Synopsis Gene Silencing by RNA Interference by : Muhammad Sohail
Download or read book Gene Silencing by RNA Interference written by Muhammad Sohail and published by CRC Press. This book was released on 2004-08-27 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.
Book Synopsis Advanced Delivery and Therapeutic Applications of RNAi by : Kun Cheng
Download or read book Advanced Delivery and Therapeutic Applications of RNAi written by Kun Cheng and published by John Wiley & Sons. This book was released on 2013-04-19 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt: Commonly used by researchers to develop technologies for modifying and studying genetic process, RNA interference (RNAi) has many potential uses in medicine, biotechnology, and functional genomics. This book covers all essential aspects involved in the development of RNAi therapeutics, providing detailed guidance on the challenges and opportunities of bringing RNAi technologies from bench to clinic. It explores the design and mechanism of RNAi molecules, delivery strategies, and therapeutic applications in various diseases. Preclinical, regulatory, market, and intellectual aspects of RNAi technologies are also covered.
Book Synopsis RNA Interference, Editing, and Modification by : Jonatha M. Gott
Download or read book RNA Interference, Editing, and Modification written by Jonatha M. Gott and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a comprehensive collection of cuttingedge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification. Readily reproducible protocols for discovering new genes or altering gene expression.
Download or read book RNA Interference written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA Interference Technology by : Krishnarao Appasani
Download or read book RNA Interference Technology written by Krishnarao Appasani and published by Cambridge University Press. This book was released on 2005-01-17 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
Book Synopsis RNA Interference by : Patrick J. Paddison
Download or read book RNA Interference written by Patrick J. Paddison and published by Springer Science & Business Media. This book was released on 2008-01-01 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few years the major effect that RNAi has had in invertebrate systems is beginning to take hold in mammalian systems through both single gene knockdown experiments and genome-scale screens. In the next decade, there will no doubt be both notable successes and failures as we attempt to apply this genetic tool to various biological problems. Through the introduction of RNAi, mammalian systems have finally gained admittance to the pantheon of model genetic systems.
Book Synopsis RNA Interference: Challenges and Therapeutic Opportunities by : Sam Kennedy
Download or read book RNA Interference: Challenges and Therapeutic Opportunities written by Sam Kennedy and published by Murphy & Moore Publishing. This book was released on 2021-11-16 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA interference (RNAi) is a genetic regulatory mechanism that occurs in eukaryotic cells and regulates the activity of genes. Its main function is to suppress, silence or deactivate genes. RNAi can occur either naturally or artificially. It occurs naturally through the production of pre-miRNA. RNAi can also be induced experimentally by using double-stranded RNA (dsRNA). The synthetic dsRNA may be of two types: small hairpin RNA (shRNA) or short interfering RNA (siRNA). An enzyme known as DICER is necessary in both natural and artificial pathways for the formation of miRNA from pre-miRNA or for the formation of siRNA from shRNA. This book traces the progress of this field and highlights some of its key concepts and applications. It studies, analyses and upholds the pillars of RNA interference and its utmost significance in modern times. This book includes contributions of experts and scientists which will provide innovative insights into this field.
Download or read book RNA Silencing written by Esra Galun and published by World Scientific Publishing Company. This book was released on 2005-04-22 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on an emerging, central issue in molecular genetics and the development of eukaryotes: the control of gene expression by small species of RNA. As an exciting new field of endeavor, it is the first book by a single author to deal comprehensively with RNA silencing. The book provides the historical background of the field preceding the seminal work by Fire and associates in 1998 on the impact of small double-stranded RNA on the expression of nematode genes, which is considered the beginning of RNA silencing research. RNA silencing is described in a wide range of plants and animals including protozoa, simple metazoa, insects, non-mammalian vertebrates, and mammals. In each case the experimental results are provided with the accompanying background and with illustrations. There is also an appendix on the prospective use of RNA silencing in gene therapy, which is intended as a guide for investigators wishing to explore this possibility.
Book Synopsis RNA Silencing by : Gordon Carmichael
Download or read book RNA Silencing written by Gordon Carmichael and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book RNAi written by Gregory J. Hannon and published by . This book was released on 2003 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.
