RNA Biochemistry and Biotechnology

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Publisher : Springer Science & Business Media
ISBN 13 : 9401144850
Total Pages : 373 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis RNA Biochemistry and Biotechnology by : Jan Barciszewski

Download or read book RNA Biochemistry and Biotechnology written by Jan Barciszewski and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Biochemistry and Biotechnology describes various aspects of nucleic acid and protein structure, mainly RNA structure and proteins, interacting with specific RNA species. Papers deal with DNA protein interactions, telomerase, aminoacyl-tRNA synthetases, elongation factor Tu, DNA repair, RNA structure, NMR technology, RNA aptamer interaction of biological macromolecules with metal ions. Two papers deal with theoretical aspects of RNA structure production and computer modelling. Many papers describe the possibility of commercial application of RNA biotechnology. One article discusses the impact of direct democracy on basic science supporting biotechnology. Readership: Advanced graduate students, Ph.D. students and young scientists as well as specialists in the field.

RNA

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Publisher : Elsevier
ISBN 13 : 9780080540627
Total Pages : 354 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis RNA by : D. Söll

Download or read book RNA written by D. Söll and published by Elsevier. This book was released on 2001-12-03 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication summarizes the current status of our understanding of RNA, with particular emphasis on the chemistry of this key biological molecule. The various RNAs covered are messenger RNA, ribosomal RNA, transfer RNA and RNA enzymes (ribozymes). The different chapters detail biophysical and chemical methods to investigate RNA structure and function, the synthesis of native and modified RNAs and the latest advances in our understanding of the vast array of biological processes in which RNA is involved.

Analysis of RNA-Protein Complexes in vitro

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Publisher : Elsevier
ISBN 13 : 9780080886961
Total Pages : 248 pages
Book Rating : 4.8/5 (869 download)

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Book Synopsis Analysis of RNA-Protein Complexes in vitro by : J. Kjems

Download or read book Analysis of RNA-Protein Complexes in vitro written by J. Kjems and published by Elsevier. This book was released on 1998-11-13 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes in vitro. The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

Handbook of RNA Biochemistry

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Publisher :
ISBN 13 : 9781647403638
Total Pages : 0 pages
Book Rating : 4.4/5 (36 download)

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Book Synopsis Handbook of RNA Biochemistry by : Boston Carter

Download or read book Handbook of RNA Biochemistry written by Boston Carter and published by . This book was released on 2023-09-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-coding RNAs (ncRNAs) are RNA molecules which are not translated into proteins. They are classified into two types based on their length, including short non-coding RNAs comprising microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). They play a role in gene regulation at various levels, ranging from epigenetic gene silencing to post-transcriptional modulation of mRNA stability. The ncRNAs contribute in the regulation of cell physiology and shape cellular functions. They play an important role in brain development and function, and are also associated with the pathogenesis of psychiatric disorders. Several ncRNAs including circRNAs, miRNAs and lncRNAs have been implicated in regulatory functions. Furthermore, they are believed to play a role in Alzheimer's disease and cancer. This book explores all the important aspects of the biochemistry of RNA. It presents researches and studies performed by experts across the globe. The extensive content of this book provides the readers with a thorough understanding of this topic.

Enzymes in RNA Science and Biotechnology Part B

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Publisher : Elsevier
ISBN 13 : 0443239770
Total Pages : 348 pages
Book Rating : 4.4/5 (432 download)

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Book Synopsis Enzymes in RNA Science and Biotechnology Part B by :

Download or read book Enzymes in RNA Science and Biotechnology Part B written by and published by Elsevier. This book was released on 2023-11-02 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins. Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Updated release includes the latest information on Enzymes in RNA Science and Biotechnology

Non-Protein Coding RNAs

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Publisher : Springer Science & Business Media
ISBN 13 : 3540708405
Total Pages : 400 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Non-Protein Coding RNAs by : Nils Walter

Download or read book Non-Protein Coding RNAs written by Nils Walter and published by Springer Science & Business Media. This book was released on 2008-11-18 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 2006 Nobel Prize in Physiology or Medicine was awarded to the discoverers of RNA interference, Andrew Fire and Craig Mello. This prize, which follows “RNA” Nobels for splicing and RNA catalysis, highlights just one class of recently discovered non-protein coding RNAs. Remarkably, non-coding RNAs are thought to outnumber protein coding genes in mammals by perhaps as much as four-fold. In fact, it appears that the complexity of an organism correlates with the fraction of its genome devoted to non-protein coding RNAs. Essential biological processes as diverse as cell differentiation, suppression of infecting viruses and parasitic tra- posons, higher-level organization of eukaryotic chromosomes, and gene expression are found to be largely directed by non-protein coding RNAs. Currently, bioinformatic, high-throughput sequencing, and biochemical approaches are identifying an increasing number of these RNAs. Unfortunately, our ability to characterize the molecular details of these RNAs is significantly lacking. The biophysical study of these RNAs is an emergent field that is unraveling the molecular underpinnings of how RNA fulfills its multitude of roles in sustaining cellular life. The resulting understanding of the physical and chemical processes at the molecular level is critical to our ability to harness RNA for use in biotechnology and human therapy, a prospect that has recently spawned a multi-billion dollar industry.

Biophysics of RNA-Protein Interactions

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Publisher : Springer Nature
ISBN 13 : 1493997262
Total Pages : 249 pages
Book Rating : 4.4/5 (939 download)

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Book Synopsis Biophysics of RNA-Protein Interactions by : Chirlmin Joo

Download or read book Biophysics of RNA-Protein Interactions written by Chirlmin Joo and published by Springer Nature. This book was released on 2019-09-19 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.

RNA Nanotechnology

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Publisher : CRC Press
ISBN 13 : 9814411655
Total Pages : 452 pages
Book Rating : 4.8/5 (144 download)

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Book Synopsis RNA Nanotechnology by : Bin Wang

Download or read book RNA Nanotechnology written by Bin Wang and published by CRC Press. This book was released on 2014-04-02 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RN

RNA Methodologies

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Publisher : Elsevier
ISBN 13 : 0323916562
Total Pages : 962 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.

Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2022-11-22 with total page 962 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology Builds from basic information on RNA techniques to in-depth protocols for specific applications Features new chapters on RNA sequencing and RNA in situ hybridization Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics

RNA Methodologies

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Publisher : Academic Press
ISBN 13 : 0128046791
Total Pages : 876 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis RNA Methodologies by : Robert E. Farrell, Jr.

Download or read book RNA Methodologies written by Robert E. Farrell, Jr. and published by Academic Press. This book was released on 2017-08-11 with total page 876 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies Presents comprehensive, cutting-edge information covering all aspects of working with RNA Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application Presents multiple avenues for addressing the same experimental goals

Applied RNA Bioscience

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Publisher : Springer
ISBN 13 : 981108372X
Total Pages : 286 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Applied RNA Bioscience by : Seiji Masuda

Download or read book Applied RNA Bioscience written by Seiji Masuda and published by Springer. This book was released on 2018-04-10 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: The focus of this book is to introduce up-to-date information on applications and practical use of RNA for agriculture, biotechnology and medicine. It provides unique ideas, tools, and methods in detail from a variety of scientific and technical disciplines. RNA science has progressed enormously in recent decades, and vast amounts of information on RNA functions and their regulatory mechanisms are becoming available. Such a progress opened the door to an age of practical application of RNA in many fields including agriculture, plant science, medical science, brewing and fermentation technology, and material production. This book inspires its readership and contributes to not only expansion in application of RNA but also to basic research.

RNA Methodologies

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Publisher : Academic Press
ISBN 13 : 0080884954
Total Pages : 742 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.

Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Academic Press. This book was released on 2009-08-31 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center Includes classic and contemporary techniques useful for all labs

RNA Motifs and Regulatory Elements

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Publisher : Springer Science & Business Media
ISBN 13 : 3642562981
Total Pages : 234 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis RNA Motifs and Regulatory Elements by : Thomas Dandekar

Download or read book RNA Motifs and Regulatory Elements written by Thomas Dandekar and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Motifs and Regulatory Elements is the new edition of the successful book, "Regulatory RNA". It alerts the reader to the importance of regulatory RNA elements for the many different areas of cellular life. The computational and experimental methods and tools to search for new interesting regulatory RNA structures are explained and compared. The knowledge on regulatory RNA structures and elements already available is concisely summarized as well as catalogued. In addition, interesting RNA elements are analyzed in detail regarding their dynamics, regulation, and as a dominant topic of current resarch in molecular biology, including areas such as RNA mediated regulation of gene-expression, DNA/RNA chip data, and ribozymes, splicing, or telomerases in aging. Medical implications are also covered. Future progress and research are finally outlined.

RNA Interference

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Publisher : Elsevier
ISBN 13 : 0080455395
Total Pages : 496 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis RNA Interference by : David R. Engelke

Download or read book RNA Interference written by David R. Engelke and published by Elsevier. This book was released on 2005-02-28 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences

Enzymes in RNA Science and Biotechnology

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Publisher : Elsevier
ISBN 13 : 0443157707
Total Pages : 300 pages
Book Rating : 4.4/5 (431 download)

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Book Synopsis Enzymes in RNA Science and Biotechnology by :

Download or read book Enzymes in RNA Science and Biotechnology written by and published by Elsevier. This book was released on 2023-11 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, highlights new advances in the field, including chapters on Reverse transcriptase Part I (discovery, preparation, general utilization, MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more, Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution, Reverse transcriptase Part II (RNA structure mapping and determination), RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays, tRNA Structure-seq in vivo and in droplets, Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq, and more.

RNA, the Epicenter of Genetic Information

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Author :
Publisher : CRC Press
ISBN 13 : 1000623114
Total Pages : 879 pages
Book Rating : 4.0/5 (6 download)

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Book Synopsis RNA, the Epicenter of Genetic Information by : John Mattick

Download or read book RNA, the Epicenter of Genetic Information written by John Mattick and published by CRC Press. This book was released on 2022-09-20 with total page 879 pages. Available in PDF, EPUB and Kindle. Book excerpt: The origin story and emergence of molecular biology is muddled. The early triumphs in bacterial genetics and the complexity of animal and plant genomes complicate an intricate history. This book documents the many advances, as well as the prejudices and founder fallacies. It highlights the premature relegation of RNA to simply an intermediate between gene and protein, the underestimation of the amount of information required to program the development of multicellular organisms, and the dawning realization that RNA is the cornerstone of cell biology, development, brain function and probably evolution itself. Key personalities, their hubris as well as prescient predictions are richly illustrated with quotes, archival material, photographs, diagrams and references to bring the people, ideas and discoveries to life, from the conceptual cradles of molecular biology to the current revolution in the understanding of genetic information. Key Features Documents the confused early history of DNA, RNA and proteins - a transformative history of molecular biology like no other. Integrates the influences of biochemistry and genetics on the landscape of molecular biology. Chronicles the important discoveries, preconceptions and misconceptions that retarded or misdirected progress. Highlights major pioneers and contributors to molecular biology, with a focus on RNA and noncoding DNA. Summarizes the mounting evidence for the central roles of non-protein-coding RNA in cell and developmental biology. Provides a thought-provoking retrospective and forward-looking perspective for advanced students and professional researchers. The Open Access version of this book, available at www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license.

RNA Technologies and Their Applications

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Publisher : Springer Science & Business Media
ISBN 13 : 3642121683
Total Pages : 460 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis RNA Technologies and Their Applications by : Volker A. Erdmann

Download or read book RNA Technologies and Their Applications written by Volker A. Erdmann and published by Springer Science & Business Media. This book was released on 2010-09-02 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA technologies are the driving forces of modern medicine and biotechnology. They combine the fields of biochemistry, chemistry, molecular biology, cell biology, physics, nanotechnology and bioinformatics. The combination of these topics is set to revolutionize the medicine of tomorrow. After more than 15 years of extensive research in the field of RNA technologies, the first therapeutics are ready to reach the first patients. Thus we are witnessing the birth of a very exciting time in the development of molecular medicine, which will be based on the methods of RNA technologies. This volume is the first of a series. It covers various aspects of RNA interference and microRNAs, although antisense RNA applications, hammerhead ribozyme structure and function as well as non-coding RNAs are also discussed. The authors are internationally highly respected experts in the field of RNA technologies.