Oxidative Stress in Plants

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Author :
Publisher : CRC Press
ISBN 13 : 0203303148
Total Pages : 417 pages
Book Rating : 4.2/5 (33 download)

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Book Synopsis Oxidative Stress in Plants by : Dirk Inze

Download or read book Oxidative Stress in Plants written by Dirk Inze and published by CRC Press. This book was released on 2001-10-18 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants depend on physiological mechanisms to combat adverse environmental conditions, such as pathogen attack, wounding, drought, cold, freezing, salt, UV, intense light, heavy metals and SO2. Many of these cause excess production of active oxygen species in plant cells. Plants have evolved complex defense systems against such oxidative stress. The

Reactive Oxygen Species in Plants

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 1119324947
Total Pages : 611 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis Reactive Oxygen Species in Plants by : Vijay Pratap Singh

Download or read book Reactive Oxygen Species in Plants written by Vijay Pratap Singh and published by John Wiley & Sons. This book was released on 2017-10-11 with total page 611 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the basics of ROS metabolism in plants and examines the broad range of ROS signaling mechanisms New discoveries about the effects of reactive oxygen species (ROS) on plants have turned ROS from being considered a bane into a boon, because their roles have been discovered in many plant developmental processes as signaling molecules. This comprehensive book teaches about the role of ROS metabolism in plants and how they affect various developmental processes. It also discusses in detail the advancements made in understanding the ROS signaling. Reactive Oxygen Species in Plants: Boon Or Bane - Revisiting the Role of ROS begins by presenting the basic introduction to ROS and deciphers the detailed knowledge in ROS research. It then examines the broad range of ROS signaling mechanisms as well as how they may be beneficial for plants and human beings. This book also describes both the bane and boon aspects of ROS with their impact on plants, and how the recent revelations have compelled us to rethink ROS turning from stressors to plant regulators. ● Compiles, for the first time, the wholesome knowledge in ROS research and their cellular signaling ● Includes new discoveries and in-depth discussions about the advancements made in the field ● Discusses reactive oxygen species which are involved in a broad range of biological processes Reactive Oxygen Species in Plants: Boon Or Bane - Revisiting the Role of ROS will help scientists to utilize the functions of ROS signaling for plants and also enable readers to gain a deeper knowledge of ROS research and signaling. It is highly recommended for researchers, scientists, and academicians in plant science as well for advanced undergraduate and postgraduate students.

Reactive Oxygen Species in Plant Biology

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Author :
Publisher : Springer
ISBN 13 : 8132239415
Total Pages : 197 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Reactive Oxygen Species in Plant Biology by : Soumen Bhattacharjee

Download or read book Reactive Oxygen Species in Plant Biology written by Soumen Bhattacharjee and published by Springer. This book was released on 2019-05-10 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the latest advances made in the niche area of Reactive Oxygen Species and Redox processes in plants. It offers a valuable guide for researchers and students alike, providing insights into sensing, detox scavenging, the role in oxidative deterioration, and signaling associated with redox-regulatory processes in plants. The book also dramatically demonstrates how these amazingly resourceful molecular species and radicals are poised at the core of a sophisticated network of signaling pathways, and act as vital regulators of plants’ cell physiology and cellular responses to the environment. The molecular language associated with ROS-mediated signal transduction, which produces modulations in gene expression that determine plants’ stress acclamatory performance, is also discussed. The book subsequently provides information on current trends in redox proteomics and genomics, which include efforts to gain a fuller understanding of these redox players’ role in cellular processes, and to further the application of this knowledge to technology and agriculture. Given its scope and format, the book offers a valuable asset for students of Plant Sciences, Agriculture, and Molecular Biology, as well as readers engaged in research on and teaching ROS Biology.

Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress

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Publisher : Springer
ISBN 13 : 9789811353512
Total Pages : 329 pages
Book Rating : 4.3/5 (535 download)

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Book Synopsis Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress by : M. Iqbal R. Khan

Download or read book Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress written by M. Iqbal R. Khan and published by Springer. This book was released on 2018-12-12 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present edited book is an attempt to update the state of art of the knowledge on metabolism of ROS and antioxidants and their relationship in plant adaptation to abiotic stresses involving physiological, biochemical and molecular processes. The chapters are much focused on the current climate issues and how ROS metabolism can manipulate with antioxidant system to accelerate detoxification mechanism. It will enhance the mechanistic understanding on ROS and antioxidants system and will pave the path for agricultural scientists in developing tolerant crops to achieve sustainability under the changing environmental conditions. The increase in abiotic stress factors has become a major threat to sustainability of crop production. This situation has led to think ways which can help to come out with potential measures; for which it is necessary to understand the influence of abiotic stress factors on crops performance and the mechanisms by which these factors impact plants. It has now become evident that abiotic stress impacts negatively on plant growth and development at every stage of plant’s life. Plants adapt to the changing environment with the adjustment at physiological, biochemical and molecular levels. The possible mechanisms involved in the negative effects of abiotic stress factors are excess production of reactive oxygen species (ROS). They alter physiological and molecular mechanisms leading to poor performance of plants. Plants however, are able to cope with these adverse effects by inducing antioxidant systems as the priority. Nevertheless, the dual role of ROS has now been ascertained which provides an evidence for regulation of plant metabolism positively on a concentration-dependent manner. Under conditions of high ROS production, the antioxidant system plays a major role in diminishing the effects of ROS. Thus, ROS production and antioxidant system are interwoven with abiotic stress conditions. The antioxidants have the capacity to hold the stability in metabolism in order to avoid disruption due to environmental disturbances.

Advances in Plant Ecophysiology Techniques

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Publisher : Springer
ISBN 13 : 3319932330
Total Pages : 491 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis Advances in Plant Ecophysiology Techniques by : Adela M. Sánchez-Moreiras

Download or read book Advances in Plant Ecophysiology Techniques written by Adela M. Sánchez-Moreiras and published by Springer. This book was released on 2018-08-17 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.

Abiotic Stress-Mediated Sensing and Signaling in Plants: An Omics Perspective

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Publisher : Springer
ISBN 13 : 9811074798
Total Pages : 358 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Abiotic Stress-Mediated Sensing and Signaling in Plants: An Omics Perspective by : Sajad Majeed Zargar

Download or read book Abiotic Stress-Mediated Sensing and Signaling in Plants: An Omics Perspective written by Sajad Majeed Zargar and published by Springer. This book was released on 2018-02-20 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural environment for plants is composed of a complex set of abiotic and biotic stresses; plant responses to these stresses are equally complex. Systems biology allows us to identify regulatory hubs in complex networks. It also examines the molecular “parts” (transcripts, proteins and metabolites) of an organism and attempts to combine them into functional networks or models that effectively describe and predict the dynamic activities of that organism in different environments. This book focuses on research advances regarding plant responses to abiotic stresses, from the physiological level to the molecular level. It highlights new insights gained from the integration of omics datasets and identifies remaining gaps in our knowledge, outlining additional focus areas for future crop improvement research. Plants have evolved a wide range of mechanisms for coping with various abiotic stresses. In many crop plants, the molecular mechanisms involved in a single type of stress tolerance have since been identified; however, in order to arrive at a holistic understanding of major and common events concerning abiotic stresses, the signaling pathways involved must also be elucidated. To date several molecules, like transcription factors and kinases, have been identified as promising candidates that are involved in crosstalk between stress signalling pathways. However, there is a need to better understand the tolerance mechanisms for different abiotic stresses by thoroughly grasping the signalling and sensing mechanisms involved. Accordingly, this book covers a range of topics, including the impacts of different abiotic stresses on plants, the molecular mechanisms leading to tolerance for different abiotic stresses, signaling cascades revealing cross-talk among various abiotic stresses, and elucidation of major candidate molecules that may provide abiotic stress tolerance in plants.

Plant Stress Tolerance

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Publisher : Springer Nature
ISBN 13 : 1071639730
Total Pages : 293 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Plant Stress Tolerance by : Ramanjulu Sunkar

Download or read book Plant Stress Tolerance written by Ramanjulu Sunkar and published by Springer Nature. This book was released on with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant Abiotic Stress Tolerance

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Author :
Publisher : Springer
ISBN 13 : 3030061183
Total Pages : 506 pages
Book Rating : 4.0/5 (3 download)

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Book Synopsis Plant Abiotic Stress Tolerance by : Mirza Hasanuzzaman

Download or read book Plant Abiotic Stress Tolerance written by Mirza Hasanuzzaman and published by Springer. This book was released on 2019-04-04 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants have to manage a series of environmental stresses throughout their entire lifespan. Among these, abiotic stress is the most detrimental; one that is responsible for nearly 50% of crop yield reduction and appears to be a potential threat to global food security in coming decades. Plant growth and development reduces drastically due to adverse effects of abiotic stresses. It has been estimated that crop can exhibit only 30% of their genetic potentiality under abiotic stress condition. So, this is a fundamental need to understand the stress responses to facilitate breeders to develop stress resistant and stress tolerant cultivars along with good management practices to withstand abiotic stresses. Also, a holistic approach to understanding the molecular and biochemical interactions of plants is important to implement the knowledge of resistance mechanisms under abiotic stresses. Agronomic practices like selecting cultivars that is tolerant to wide range of climatic condition, planting date, irrigation scheduling, fertilizer management could be some of the effective short-term adaptive tools to fight against abiotic stresses. In addition, “system biology” and “omics approaches” in recent studies offer a long-term opportunity at the molecular level in dealing with abiotic stresses. The genetic approach, for example, selection and identification of major conditioning genes by linkage mapping and quantitative trait loci (QTL), production of mutant genes and transgenic introduction of novel genes, has imparted some tolerant characteristics in crop varieties from their wild ancestors. Recently research has revealed the interactions between micro-RNAs (miRNAs) and plant stress responses exposed to salinity, freezing stress and dehydration. Accordingly transgenic approaches to generate stress-tolerant plant are one of the most interesting researches to date. This book presents the recent development of agronomic and molecular approaches in conferring plant abiotic stress tolerance in an organized way. The present volume will be of great interest among research students and teaching community, and can also be used as reference material by professional researchers.

Oxidative Damage to Plants

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Publisher : Academic Press
ISBN 13 : 0128004606
Total Pages : 666 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Oxidative Damage to Plants by : Parvaiz Ahmad

Download or read book Oxidative Damage to Plants written by Parvaiz Ahmad and published by Academic Press. This book was released on 2014-01-29 with total page 666 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions that review research on this topic throughout the world, Oxidative Damage to Plants covers key areas of discovery, from the generation of reactive oxygen species (ROSs), their mechanisms, quenching of these ROSs through enzymatic and non-enzymatic antioxidants, and detailed aspects of such antioxidants as SOD and CAT. Environmental stress is responsible for the generation of oxidative stress, which causes oxidative damage to biomolecules and hence reduces crop yield. To cope up with these problems, scientists have to fully understand the generation of reactive oxygen species, its impact on plants and how plants will be able to withstand these stresses. - Provides invaluable information about the role of antioxidants in alleviating oxidative stress - Examines both the negative effects (senescence, impaired photosynthesis and necrosis) and positive effects (crucial role that superoxide plays against invading microbes) of ROS on plants - Features contributors from a variety of regions globally

Heavy Metal Stress in Plants

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Publisher : Springer Science & Business Media
ISBN 13 : 3642384692
Total Pages : 245 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis Heavy Metal Stress in Plants by : Dharmendra K. Gupta

Download or read book Heavy Metal Stress in Plants written by Dharmendra K. Gupta and published by Springer Science & Business Media. This book was released on 2013-08-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants possess a range of potential cellular mechanisms that may be involved in the detoxification of heavy metals and thus tolerance to metal stress. Metal toxicity causes multiple direct and indirect effects in plants that concern practically all physiological functions. The main purpose of this book is to present comprehensive and concise information on recent advances in the field of metal transport and how genetic diversity affects heavy metal transport in plants. Other key futures of the book are related to metal toxicity and detoxification mechanisms, biochemical tools for HM remediation processes, molecular mechanisms for HM detoxification, how metallomics and metalloproteomics are affected by heavy metal stress in plants, and the role of ROS metabolism in the alleviation of heavy metals. Some chapters also focus on recent developments in the field of phytoremediation. Overall the book presents in-depth information and the most essential advances in the field of heavy metal toxicity in plants in recent years.

Handbook of Plant and Crop Physiology

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Publisher : CRC Press
ISBN 13 : 082474134X
Total Pages : 997 pages
Book Rating : 4.8/5 (247 download)

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Book Synopsis Handbook of Plant and Crop Physiology by : Mohammad Pessarakli

Download or read book Handbook of Plant and Crop Physiology written by Mohammad Pessarakli and published by CRC Press. This book was released on 2001-09-18 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions from over 70 international experts, this reference provides comprehensive coverage of plant physiological stages and processes under both normal and stressful conditions. It emphasizes environmental factors, climatic changes, developmental stages, and growth regulators as well as linking plant and crop physiology to the production of food, feed, and medicinal compounds. Offering over 300 useful tables, equations, drawings, photographs, and micrographs, the book covers cellular and molecular aspects of plant and crop physiology, plant and crop physiological responses to heavy metal concentration and agrichemicals, computer modeling in plant physiology, and more.

Antioxidant Defense in Plants

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Publisher : Springer Nature
ISBN 13 : 9811679819
Total Pages : 458 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis Antioxidant Defense in Plants by : Tariq Aftab

Download or read book Antioxidant Defense in Plants written by Tariq Aftab and published by Springer Nature. This book was released on 2022-05-04 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book highlights the molecular basis of various enzymatic and non-enzymatic antioxidants, defense mechanisms and adaptation strategies employed by plants to avoid the stressful conditions. Special focus is given to gene expression, omics and other latest technologies such as CRISPR-Cas mediated genome editing applications for defense related studies in plants. Environmental stresses such as drought, salinity or floods etc. induce the generation of reactive oxygen species (ROS) which causes severe damage to cell membrane integrity by accelerating lipid peroxidation. To counteract the detrimental effect of ROS, plants are inherited with an intricate and vibrant antioxidant defense system, comprised of enzymatic (catalase, peroxidase, superoxide dismutase, glutathione reductase, glutathione S-transferase, guaiacol peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase etc.), and non-enzymatic (glutathione, ascorbate, α-tocopherol, carotenoids, flavonoids etc.) antioxidants, which scavenge and/or reduce excess ROS and improve plant tolerance to various stresses. Stress tolerance in most crop plants is positively correlated with an efficient antioxidant system. Therefore, studying the efficiency of antioxidant defense systems in plants is necessary for facilitating the plant’s nature of adaptation against challenging environments. This book is of interest to teachers, researchers and academic experts. Also, the book serves as additional reading material for undergraduate and graduate students of biotechnology and molecular biology of plants.

Regulation of Photosynthesis

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Publisher : Springer Science & Business Media
ISBN 13 : 0306481480
Total Pages : 624 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Regulation of Photosynthesis by : Eva-Mari Aro

Download or read book Regulation of Photosynthesis written by Eva-Mari Aro and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the expression of photosynthesis related genes including regulation both at transcriptional and translational levels. It reviews biogenesis, turnover, and senescence of thylakoid pigment protein complexes and highlights some crucial regulatory steps in carbon metabolism.

Reactive Oxygen Species (ROS), Nanoparticles, and Endoplasmic Reticulum (ER) Stress-Induced Cell Death Mechanisms

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Publisher : Academic Press
ISBN 13 : 0128224967
Total Pages : 782 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Reactive Oxygen Species (ROS), Nanoparticles, and Endoplasmic Reticulum (ER) Stress-Induced Cell Death Mechanisms by : Loutfy H. Madkour

Download or read book Reactive Oxygen Species (ROS), Nanoparticles, and Endoplasmic Reticulum (ER) Stress-Induced Cell Death Mechanisms written by Loutfy H. Madkour and published by Academic Press. This book was released on 2020-06-27 with total page 782 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reactive Oxygen Species (ROS), Nanoparticles, and Endoplasmic Reticulum (ER) Stress-Induced Cell Death Mechanisms presents the role of ROS?mediated pathways cellular signaling stress, endoplasmic reticulum (ER) stress, oxidative stress, oxidative damage, nanomaterials, and the mechanisms by which metalloids and nanoparticles induce their toxic effects. The book covers the ecotoxicology of environmental heavy metal ions and free radicals on macromolecules cells organisms, heavy metals?induced cell responses, oxidative stress, the source of oxidants, and the roles of ROS, oxidative stress and oxidative damage mechanisms. It also examines the nanotoxicity, cytotoxicity and genotoxicity mechanisms of nanomaterials and the effects of nanoparticle interactions. Antioxidant defense therapy and strategies for treatment round out the book, making it an ideal resource for researchers and professional scientists in toxicology, environmental chemistry, environmental science, nanomaterials and the pharmaceutical sciences. - Covers the ecotoxicology of environmental heavy metal ions and the interactions between specific heavy metals?induced cell responses and oxidative stress - Provides a better understanding of the mechanism of nanomaterial-induced toxicity as a first defense for hazard prevention - Covers recent advances in new nanomedication technologies for the effects of NPs on oxidative stress, ROS and ER stress - Discusses the effects of interactions between antioxidant defense therapy, ROS and strategies for treatment

Reactive Oxygen Species (ROS) in Living Cells

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Author :
Publisher : BoD – Books on Demand
ISBN 13 : 1789231345
Total Pages : 218 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Reactive Oxygen Species (ROS) in Living Cells by : Filip Cristiana

Download or read book Reactive Oxygen Species (ROS) in Living Cells written by Filip Cristiana and published by BoD – Books on Demand. This book was released on 2018-05-23 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oxygen represents only 20% of the Earth's atmosphere, yet it is vital for the survival of aerobic organisms. There is a dark part of the use of oxygen that consists in generating reactive species that are potentially harmful to living organisms. Moreover, reactive oxygen species can combine with nitrogen derivatives and generate many other reactive species. Thus, living organisms are continuously assaulted by reactive species from external or internal sources. However, the real danger comes in the case of high concentrations and prolonged exposure to these species. This book presents an image of the mechanisms of action of reactive species and emphasizes their involvement in diseases. Inflammation and cancer are examined to determine when and how reactive species turn the evolution of a benign process to a malignant one. Some answers may come from recent studies indicating that reactive species are responsible for epigenetic changes.

Abiotic Stress Responses in Plants

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Publisher : Springer Science & Business Media
ISBN 13 : 146140634X
Total Pages : 475 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Abiotic Stress Responses in Plants by : Parvaiz Ahmad

Download or read book Abiotic Stress Responses in Plants written by Parvaiz Ahmad and published by Springer Science & Business Media. This book was released on 2011-11-16 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abiotic stress cause changes in soil-plant-atmosphere continuum and is responsible for reduced yield in several major crops. Therefore, the subject of abiotic stress response in plants - metabolism, productivity and sustainability - is gaining considerable significance in the contemporary world. Abiotic stress is an integral part of “climate change,” a complex phenomenon with a wide range of unpredictable impacts on the environment. Prolonged exposure to these abiotic stresses results in altered metabolism and damage to biomolecules. Plants evolve defense mechanisms to tolerate these stresses by upregulation of osmolytes, osmoprotectants, and enzymatic and non-enzymatic antioxidants, etc. This volume deals with abiotic stress-induced morphological and anatomical changes, abberations in metabolism, strategies and approaches to increase salt tolerance, managing the drought stress, sustainable fruit production and postharvest stress treatments, role of glutathione reductase, flavonoids as antioxidants in plants, the role of salicylic acid and trehalose in plants, stress-induced flowering. The role of soil organic matter in mineral nutrition and fatty acid profile in response to heavy metal stress are also dealt with. Proteomic markers for oxidative stress as a new tools for reactive oxygen species and photosynthesis research, abscisic acid signaling in plants are covered with chosen examples. Stress responsive genes and gene products including expressed proteins that are implicated in conferring tolerance to the plant are presented. Thus, this volume would provides the reader with a wide spectrum of information including key references and with a large number of illustrations and tables. Dr. Parvaiz is Assistant Professor in Botany at A.S. College, Srinagar, Jammu and Kashmir, India. He has completed his post-graduation in Botany in 2000 from Jamia Hamdard New Delhi India. After his Ph.D from the Indian Institute of Technology (IIT) Delhi, India in 2007 he joined the International Centre for Genetic Engineering and Biotechnology, New Delhi. He has published more than 20 research papers in peer reviewed journals and 4 book chapters. He has also edited a volume which is in press with Studium Press Pvt. India Ltd., New Delhi, India. Dr. Parvaiz is actively engaged in studying the molecular and physio-biochemical responses of different plants (mulberry, pea, Indian mustard) under environmental stress. Prof. M.N.V. Prasad is a Professor in the Department of Plant Sciences at the University of Hyderabad, India. He received B.Sc. (1973) and M.Sc. (1975) degrees from Andhra University, India, and the Ph.D. degree (1979) in botany from the University of Lucknow, India. Prasad has published 216 articles in peer reviewed journals and 82 book chapters and conference proceedings in the broad area of environmental botany and heavy metal stress in plants. He is the author, co-author, editor, or co-editor for eight books. He is the recipient of Pitamber Pant National Environment Fellowship of 2007 awarded by the Ministry of Environment and Forests, Government of India.

Oxidative Stress in Plants

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Author :
Publisher :
ISBN 13 : 9789381141021
Total Pages : 0 pages
Book Rating : 4.1/5 (41 download)

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Book Synopsis Oxidative Stress in Plants by : Naser A. Anjum

Download or read book Oxidative Stress in Plants written by Naser A. Anjum and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examines major recent advances through physiological and molecular studies on all aspects of the causes and consequences of oxidative stress, and discusses and suggests potential strategies for enhancing tolerance to oxidative stress in detail in the light of recent advances in molecular biology.