Quantification Of Circulating Proteins

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Quantification of Circulating Proteins

Author : Wim Th. Hermens
Publisher : Springer Science & Business Media
ISBN 13 : 9400976607
Total Pages : 260 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Quantification of Circulating Proteins by : Wim Th. Hermens

Download or read book Quantification of Circulating Proteins written by Wim Th. Hermens and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Less than 50 years ago it was discovered that steady-state protein concentrations in plasma are the net result of continuous elimination and synthesis of protein molecules. The first quanti tative studies on the turnover and distribution of plasma pro teins were made around 1950, after the introduction of radio labeled protein preparations. Around 1970, another development in quantitative interpre tation of circulating proteins was initiated in clinical enzy mology. Estimation of cumulative release into plasma of cellular enzymes can be helpful in a variety of diseases to assess the extent of tissue damage and to evaluate therapy. Enzymes can be considered as biological tracers, i.e. minute quantities of protein can be accurately determined by their spe cific catalytic activities. However, radioactive tracers permit direct estimates of turnover and distr ibution by measurement of excreted radioactivity, possibilities that are not available for enzymes. Consequently, only a few techniques used in tracer studies with radiolabeled proteins can be applied to circulating tissue enzymes and this may explain the lack of communication between the fields of plasma protein metabolism and quantitative clinical enzymology. In the present study a summary is given of the basic methods used in both fields, with emphasis on the equivalence of various models and formalisms used by different authors. It is shown that major limitations in the study of circulating tissue enzymes can be overcome if two different, but simultaneously released, en zymes can be measured. The resulting method will also be applied to plasma protein metabolism.

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

Author : Ingvar Eidhammer
Publisher : John Wiley & Sons
ISBN 13 : 111849377X
Total Pages : 290 pages
Book Rating : 4.1/5 (184 download)

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Book Synopsis Computational and Statistical Methods for Protein Quantification by Mass Spectrometry by : Ingvar Eidhammer

Download or read book Computational and Statistical Methods for Protein Quantification by Mass Spectrometry written by Ingvar Eidhammer and published by John Wiley & Sons. This book was released on 2012-12-10 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive introduction to data analysis in quantitative proteomics This book provides all the necessary knowledge about mass spectrometry based proteomics methods and computational and statistical approaches to pursue the planning, design and analysis of quantitative proteomics experiments. The author’s carefully constructed approach allows readers to easily make the transition into the field of quantitative proteomics. Through detailed descriptions of wet-lab methods, computational approaches and statistical tools, this book covers the full scope of a quantitative experiment, allowing readers to acquire new knowledge as well as acting as a useful reference work for more advanced readers. Computational and Statistical Methods for Protein Quantification by Mass Spectrometry: Introduces the use of mass spectrometry in protein quantification and how the bioinformatics challenges in this field can be solved using statistical methods and various software programs. Is illustrated by a large number of figures and examples as well as numerous exercises. Provides both clear and rigorous descriptions of methods and approaches. Is thoroughly indexed and cross-referenced, combining the strengths of a text book with the utility of a reference work. Features detailed discussions of both wet-lab approaches and statistical and computational methods. With clear and thorough descriptions of the various methods and approaches, this book is accessible to biologists, informaticians, and statisticians alike and is aimed at readers across the academic spectrum, from advanced undergraduate students to post doctorates entering the field.

Proteopathic Seeds and Neurodegenerative Diseases

Author : Mathias Jucker
Publisher : Springer Science & Business Media
ISBN 13 : 3642354912
Total Pages : 163 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis Proteopathic Seeds and Neurodegenerative Diseases by : Mathias Jucker

Download or read book Proteopathic Seeds and Neurodegenerative Diseases written by Mathias Jucker and published by Springer Science & Business Media. This book was released on 2013-03-27 with total page 163 pages. Available in PDF, EPUB and Kindle. Book excerpt: The misfolding and aggregation of specific proteins is an early and obligatory event in many of the age-related neurodegenerative diseases of humans. The initial cause of this pathogenic cascade and the means whereby disease spreads through the nervous system, remain uncertain. A recent surge of research, first instigated by pathologic similarities between prion disease and Alzheimer’s disease, increasingly implicates the conversion of disease-specific proteins into an aggregate-prone b-sheet-rich state as the prime mover of the neurodegenerative process. This prion-like corruptive protein templating or seeding now characterizes such clinically and etiologically diverse neurological disorders as Alzheimer ́s disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. Understanding the misfolding, aggregation, trafficking and pathogenicity of the affected proteins could therefore reveal universal pathomechanistic principles for some of the most devastating and intractable human brain disorders. It is time to accept that the prion concept is no longer confined to prionoses but is a promising concept for the understanding and treatment of a remarkable variety of diseases that afflict primarily our aging society.

Human Plasma Lipoproteins

Author : Jean-Charles Fruchart
Publisher :
ISBN 13 :
Total Pages : 424 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Human Plasma Lipoproteins by : Jean-Charles Fruchart

Download or read book Human Plasma Lipoproteins written by Jean-Charles Fruchart and published by . This book was released on 1989 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Quantitative Methods in Proteomics

Author : Katrin Marcus
Publisher : Humana Press
ISBN 13 : 9781617798849
Total Pages : 539 pages
Book Rating : 4.7/5 (988 download)

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Book Synopsis Quantitative Methods in Proteomics by : Katrin Marcus

Download or read book Quantitative Methods in Proteomics written by Katrin Marcus and published by Humana Press. This book was released on 2012-06-08 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein modifications and changes made to them, as well as the quantities of expressed proteins, can define the various functional stages of the cell. Accordingly, perturbations can lead to various diseases and disorders. As a result, it has become paramount to be able to detect and monitor post-translational modifications and to measure the abundance of proteins within the cell with extreme sensitivity. While protein identification is an almost routine requirement nowadays, reliable techniques for quantifying unmodified proteins (including those that escape detection under standard conditions, such as protein isoforms and membrane proteins) is not routine. Quantitative Methods in Proteomics gives a detailed survey of topics and methods on the principles underlying modern protein analysis, from statistical issues when planning proteomics experiments, to gel-based and mass spectrometry-based applications. The quantification of post-translational modifications is also addressed, followed by the “hot” topics of software and data analysis, as well as various overview chapters which provide a comprehensive overview of existing methods in quantitative proteomics. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Quantitative Methods in Proteomics serves as a comprehensive and competent overview of the important and still growing field of quantitative proteomics.

ADME and Translational Pharmacokinetics / Pharmacodynamics of Therapeutic Proteins

Author : Honghui Zhou
Publisher : John Wiley & Sons
ISBN 13 : 1118898745
Total Pages : 476 pages
Book Rating : 4.1/5 (188 download)

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Book Synopsis ADME and Translational Pharmacokinetics / Pharmacodynamics of Therapeutic Proteins by : Honghui Zhou

Download or read book ADME and Translational Pharmacokinetics / Pharmacodynamics of Therapeutic Proteins written by Honghui Zhou and published by John Wiley & Sons. This book was released on 2015-10-26 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: With an emphasis on the fundamental and practical aspects of ADME for therapeutic proteins, this book helps readers strategize, plan and implement translational research for biologic drugs. • Details cutting-edge ADME (absorption, distribution, metabolism and excretion) and PKPD (pharmacokinetic / pharmacodynamics) modeling for biologic drugs • Combines theoretical with practical aspects of ADME in biologic drug discovery and development and compares innovator biologics with biosimilar biologics and small molecules with biologics, giving a lessons-learned perspective • Includes case studies about leveraging ADME to improve biologics drug development for monoclonal antibodies, fusion proteins, pegylated proteins, ADCs, bispecifics, and vaccines • Presents regulatory expectations and industry perspectives for developing biologic drugs in USA, EU, and Japan • Provides mechanistic insight into biodistribution and target-driven pharmacokinetics in important sites of action such as tumors and the brain

Clinical Molecular Diagnostics

Author : Shiyang Pan
Publisher : Springer Nature
ISBN 13 : 9811610371
Total Pages : 889 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis Clinical Molecular Diagnostics by : Shiyang Pan

Download or read book Clinical Molecular Diagnostics written by Shiyang Pan and published by Springer Nature. This book was released on 2021-07-08 with total page 889 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the discovery of molecular biomarkers, the development of laboratory testing techniques and their clinical applications, focusing on basic research to clinical practice. It introduces new and crucial knowledge and ethics of clinical molecular diagnosis. This book emphasizes the applications of clinical molecular diagnostic test on health management, especially from different diseased organs. It lets readers to understand and realize precision healthcare.

Mass Spectrometry for the Clinical Laboratory

Author : Hari Nair
Publisher : Academic Press
ISBN 13 : 0128009926
Total Pages : 306 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Mass Spectrometry for the Clinical Laboratory by : Hari Nair

Download or read book Mass Spectrometry for the Clinical Laboratory written by Hari Nair and published by Academic Press. This book was released on 2016-11-02 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab. Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab

Targeted Biomarker Quantitation by LC-MS

Author : Naidong Weng
Publisher : John Wiley & Sons
ISBN 13 : 1119413060
Total Pages : 464 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Targeted Biomarker Quantitation by LC-MS by : Naidong Weng

Download or read book Targeted Biomarker Quantitation by LC-MS written by Naidong Weng and published by John Wiley & Sons. This book was released on 2017-07-05 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book to offer a blueprint for overcoming the challenges to successfully quantifying biomarkers in living organisms The demand among scientists and clinicians for targeted quantitation experiments has experienced explosive growth in recent years. While there are a few books dedicated to bioanalysis and biomarkers in general, until now there were none devoted exclusively to addressing critical issues surrounding this area of intense research. Target Biomarker Quantitation by LC-MS provides a detailed blueprint for quantifying biomarkers in biological systems. It uses numerous real-world cases to exemplify key concepts, all of which were carefully selected and presented so as to allow the concepts they embody to be easily expanded to future applications, including new biomarker development. Target Biomarker Quantitation by LC-MS primarily focuses on the assay establishment for biomarker quantitation—a critical issue rarely treated in depth. It offers comprehensive coverage of three core areas of biomarker assay establishment: the relationship between the measured biomarkers and their intended usage; contemporary regulatory requirements for biomarker assays (a thorough understanding of which is essential to producing a successful and defendable submission); and the technical challenges of analyzing biomarkers produced inside a living organism or cell. Covers the theory of and applications for state-of-the-art mass spectrometry and chromatography and their applications in biomarker analysis Features real-life examples illustrating the challenges involved in target biomarker quantitation and the innovative approaches which have been used to overcome those challenges Addresses potential obstacles to obtain effective biomarker level and data interpretation, such as specificity establishment and sample collection Outlines a tiered approach and fit-for-purpose assay protocol for target biomarker quantitation Highlights the current state of the biomarker regulatory environment and protocol standards Target Biomarker Quantitation by LC-MS is a valuable resource for bioanalytical scientists, drug metabolism and pharmacokinetics scientists, clinical scientists, analytical chemists, and others for whom biomarker quantitation is an important tool of the trade. It also functions as an excellent text for graduate courses in pharmaceutical, biochemistry and chemistry.

Handbook of Immunoassay Technologies

Author : Sandeep K. Vashist
Publisher : Academic Press
ISBN 13 : 012811794X
Total Pages : 496 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Handbook of Immunoassay Technologies by : Sandeep K. Vashist

Download or read book Handbook of Immunoassay Technologies written by Sandeep K. Vashist and published by Academic Press. This book was released on 2018-01-02 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. Provides a valuable increase of understanding of cellular and biomedical functions Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings Discusses all multifarious aspects of immunoassays Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays

Subcellular Proteomics

Author : Eric Bertrand
Publisher : Springer Science & Business Media
ISBN 13 : 1402059434
Total Pages : 397 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Subcellular Proteomics by : Eric Bertrand

Download or read book Subcellular Proteomics written by Eric Bertrand and published by Springer Science & Business Media. This book was released on 2007-08-29 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.

Zinc Metalloproteases In Health And Disease

Author : NM Hooper
Publisher : CRC Press
ISBN 13 : 1482272768
Total Pages : 350 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Zinc Metalloproteases In Health And Disease by : NM Hooper

Download or read book Zinc Metalloproteases In Health And Disease written by NM Hooper and published by CRC Press. This book was released on 1996-07-30 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: The zinc metalloproteases are a diverse group of enzymes which are becoming increasingly important in a variety of biological systems. Their major function is to break down proteins. This text presents recent research results on the biochemistry and molecular biology of these enzymes.

Proteomic Profiling

Author : Anton Posch
Publisher :
ISBN 13 : 9781071611883
Total Pages : pages
Book Rating : 4.6/5 (118 download)

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Book Synopsis Proteomic Profiling by : Anton Posch

Download or read book Proteomic Profiling written by Anton Posch and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Role of Protein and Amino Acids in Sustaining and Enhancing Performance

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309172810
Total Pages : 448 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis The Role of Protein and Amino Acids in Sustaining and Enhancing Performance by : Institute of Medicine

Download or read book The Role of Protein and Amino Acids in Sustaining and Enhancing Performance written by Institute of Medicine and published by National Academies Press. This book was released on 1999-09-15 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a commonly held belief that athletes, particularly body builders, have greater requirements for dietary protein than sedentary individuals. However, the evidence in support of this contention is controversial. This book is the latest in a series of publications designed to inform both civilian and military scientists and personnel about issues related to nutrition and military service. Among the many other stressors they experience, soldiers face unique nutritional demands during combat. Of particular concern is the role that dietary protein might play in controlling muscle mass and strength, response to injury and infection, and cognitive performance. The first part of the book contains the committee's summary of the workshop, responses to the Army's questions, conclusions, and recommendations. The remainder of the book contains papers contributed by speakers at the workshop on such topics as, the effects of aging and hormones on regulation of muscle mass and function, alterations in protein metabolism due to the stress of injury or infection, the role of individual amino acids, the components of proteins, as neurotransmitters, hormones, and modulators of various physiological processes, and the efficacy and safety considerations associated with dietary supplements aimed at enhancing performance.

Drug Transporters

Author : Martin F. Fromm
Publisher : Springer Science & Business Media
ISBN 13 : 3642145418
Total Pages : 457 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Drug Transporters by : Martin F. Fromm

Download or read book Drug Transporters written by Martin F. Fromm and published by Springer Science & Business Media. This book was released on 2010-11-19 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is increasingly recognized that various transporter proteins are expressed throughout the body and determine absorption, tissue distribution, biliary and renal elimination of endogenous compounds and drugs and drug effects. This book will give an overview on the transporter families which are most important for drug therapy. Most chapters will focus on one transporter family highlighting tissue expression, substrates, inhibitors, knock-out mouse models and clinical studies.

Type 2 Diabetes

Author : Anthony Barnett
Publisher : Oxford University Press
ISBN 13 : 0199596174
Total Pages : 175 pages
Book Rating : 4.1/5 (995 download)

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Book Synopsis Type 2 Diabetes by : Anthony Barnett

Download or read book Type 2 Diabetes written by Anthony Barnett and published by Oxford University Press. This book was released on 2012-10-25 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition has been updated throughout to aacount for the latest trial data, including new chapters on pathophysiology, and interpretation of recent clinical trials. This pocketbook is a concise, practical guide to the diagnosis, assessment and management of Type 2 Diabetes aimed at clinicians, GPs and nurse specialists.

Peptide Antibodies

Author : Gunnar Houen
Publisher : Humana
ISBN 13 : 9781493929986
Total Pages : 0 pages
Book Rating : 4.9/5 (299 download)

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Book Synopsis Peptide Antibodies by : Gunnar Houen

Download or read book Peptide Antibodies written by Gunnar Houen and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.