Qtl Analysis Of End Use Quality And Stripe Rust Resistance Traits In A Double Haploid Soft White Wheat Population

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QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population

Author : Carmen Johanne Barkley
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (12 download)

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Book Synopsis QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population by : Carmen Johanne Barkley

Download or read book QTL Analysis of End-use Quality and Stripe Rust Resistance Traits in a Double Haploid Soft White Wheat Population written by Carmen Johanne Barkley and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat (Triticum spp.) is a crop of major economic and dietary importance. In the Pacific Northwest region of the United States, soft white wheat is the primary class of wheat grown and is an important export product for the region. Soft white wheat flour is especially important in producing products such as cookies, crackers, pancakes, noodles and pastry products. The goal of this project was to map quantitative trait loci (QTL) for end-use quality and stripe rust (Puccinia striiformis f. sp. tritici) resistance in a double haploid mapping population derived from a cross between a soft white winter club wheat, ‘Cara’, and a soft white winter lax wheat, ‘Xerpha’. The mapping population of 126 individuals was grown across 12 environments from 2013 to 2017 and rated for stripe rust resistance. In a separate experiment, it was grown in 16 different locations from 2013 to 2016 and phenotyped for 16 end-use quality traits. A linkage map of 23 linkage groups was constructed for the population using 1282 genotyping-by-sequencing (GBS) and KASP markers. QTL analysis revealed 35 significant QTL for stripe rust resistance. Two of the most interesting markers in the stripe rust resistance analysis were IWB38687 and A2927, which were each associated with three separate QTL. Analysis of the end-use quality trait data identified 22 significant QTL. Some of the most significant QTL identified for end-use quality traits had LOD scores well over five and explained over 15% of the variance in the trait they were associated with. Some of the markers identified in this study could be good targets for marker assisted selection in PNW wheat breeding programs, especially because the parents of our mapping population are commonly used in breeding programs in the PNW.

QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.)

Author : Emily Frances Klarquist
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (925 download)

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Book Synopsis QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) by : Emily Frances Klarquist

Download or read book QTL Mapping of Stripe Rust Resistance and End-use Quality in Winter Wheat (Triticum Aestivum L.) written by Emily Frances Klarquist and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

QTL Mapping of Pre-harvest Sprouting and Stripe Rust Resistance in Wheat Cultivars Danby and Tiger

Author : Mingqin Shao
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (11 download)

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Book Synopsis QTL Mapping of Pre-harvest Sprouting and Stripe Rust Resistance in Wheat Cultivars Danby and Tiger by : Mingqin Shao

Download or read book QTL Mapping of Pre-harvest Sprouting and Stripe Rust Resistance in Wheat Cultivars Danby and Tiger written by Mingqin Shao and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat yield and quality is influenced by many abiotic and biotic environmental factors. Pre-harvest sprouting (PHS) occurs when physiologically matured spikes are exposed to wet field conditions before harvest, which results in seed germination and causes significant losses in yield and end-use quality. Wheat stripe rust is one of the most important biotic factors reducing grain yield and quality. To investigate the genetic basis of the resistance to PHS and stripe rust in hard white winter wheat cultivars Danby and Tiger and develop molecular markers for marker- assisted breeding, a double haploid (DH) population, derived from those two cultivars, was genotyped with simple sequence repeats (SSR) markers and simple nucleotide polymorphism (SNP) markers. This DH population was assessed for resistance to PHS and stripe rust in both greenhouse and field experiments. For PHS, one major resistant quantitative trait locus (QTL) was consistently detected on the short arm of chromosome 3A in all three experiments conducted and explained 21.6% to 41.0% of the phenotypic variation (PVE). This QTL is corresponding to a previously cloned gene, TaPHS1. A SNP in the promoter of TaPHS1 co- segregated with PHS resistance in this mapping population. Meanwhile, two other QTLs, Qphs.hwwg-3B.1 and Qphs.hwwg-5A.1, were consistently detected on the chromosome arms 3BS and 5AL in two experiments. These two QTLs showed significant additive effects with TaPHS1 in improving PHS resistance. For stripe rust, three major QTLs were consistently detected in four out of six environments for infection type (IT) or disease severity (DS). Two of them, QYr.hwwg-2AS1 and QYr.hwwg-4BL1, contributed by the Danby allele explained up to 28.4% of PVE for IT and 60.5% of PVE for DS. The third QTL, QYr.hwwg-3BS1, contributed by the Tiger allele, had PVE values up to 14.7% for IT and 22.9% for DS. QYr.hwwg-2AS1 and QYr.hwwg- 4BL1 are likely the same resistance genes reported previously on chromosome arms 2AS and 4BL. However, QYr.hwwg-3BS1 might be different from the reported gene cluster near the distal end of 3BS where Yr57, Yr4, Yr30 and Sr2 were located. Significant additive effects on reducing IT and DS were observed among these three major QTLs. In order to pyramid multiple QTLs in breeding, user-friendly Kompetitive allele specific PCR (KASP) markers were successfully developed for several QTLs identified in this study. The QTLs and their interactions found in this study together with those novel flanking KASP markers developed will be useful not only for understanding genetic mechanisms of PHS and stripe rust resistance but also for marker- assisted breeding to improve wheat resistance to PHS and stripe rust by gene pyramiding.

Stripe Rust

Author : Xianming Chen
Publisher : Springer
ISBN 13 : 9402411119
Total Pages : 723 pages
Book Rating : 4.4/5 (24 download)

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Book Synopsis Stripe Rust by : Xianming Chen

Download or read book Stripe Rust written by Xianming Chen and published by Springer. This book was released on 2017-07-11 with total page 723 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively introduces stripe rust disease, its development and its integral control. Covering the biology, genetics, genome, and functional genomics of the pathogen, it also discusses host and non-host resistance, their interactions and the epidemiology of the disease. It is intended for scientists, postgraduates and undergraduate studying stripe rust, plant pathology, crop breeding, crop protection and agricultural science, but is also a valuable reference book for consultants and administrators in agricultural businesses and education.

Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population

Author : Wardah K. Mustahsan
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)

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Book Synopsis Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population by : Wardah K. Mustahsan

Download or read book Characterization of Stripe Rust Resistance and Yield-related Traits in an 'Overley' X 'Overland' Population written by Wardah K. Mustahsan and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat is a vital cereal crop, providing 20 % of the daily nutritional requirements for consumers worldwide. Although there has been a substantial increase in production and yield gains, food demands will still be on the rise in future decades, and wheat yields must continue to increase rapidly to meet these demands. Grain yield can be inhibited by biotic factors (pathogens), which require the development of resistant varieties against pathogens. Stripe (yellow) rust produces massive yield losses in wheat production, and ample resistance against stripe rust is achieved by pyramiding multiple resistant genes together. Yield also can be improved by characterizing the underlying mechanisms that define yield and yield-related traits. We used breeding technology tools to dissect the genetic architecture of yield-related traits, yield, and stripe rust resistance to identify genomic regions that can improve these traits in wheat germplasm and broaden the genetic resources available for breeders to develop robust breeding germplasm that can improve the profitability and resilience of wheat production. Stripe (yellow) rust, caused by Puccinia striiformis (Pst), is a devastating disease of wheat worldwide. In commercial production, stripe rust reduces forage yield, grain yield and grain quality. Yield losses caused by stripe rust range from 10% to 70%. This study was conducted using two different wheat reference genomes (IWGSC v2.1('Chinese Spring') and 'Jagger') to identify quantitative trait loci (QTL) associated with adult plant field resistance to stripe rust in the hard winter wheat RIL population, 'Overley' x 'Overland' to provide breeders with identified genomic regions associated with quantitative field resistance. Our QTL analysis identified genomic regions on 2AS (2N[superscript v]S translocation), 2BS, 2BL, and 2DL using two different mapping methods (Inclusive Composite Interval Mapping and Multi-Environmental Trial Analysis) that were associated with stripe rust resistance for both infection type and severity using genetic maps from both genomes. There were no meaningful differences in interpreting the data from the two maps. Overley contributed the resistance alleles at the 2AS and 2BL QTLs. Overland contributed the resistance alleles at the 2BS and 2DL QTLs. The 2DL QTL (QYr.hwwg-2D) will be further refined due its environmental stability for resistance. We designed PCR-based SNP marker assays to efficiently identify these genomic regions in breeding populations. Yield improvement can be facilitated by a deeper understanding of the underlying infrastructure of components that determine grain yield. We performed a QTL analysis on the population Overley x Overland, to identify genomic regions in our population can explain the variation in yield and its components in field trials using the two different reference maps. We identified major QTLs in both reference maps for yield (3BL), single kernel weight (4AL), plant height (6AS), grains per spike (4AL), thousand grain weight (4AL), and physiological maturity (2BS). The Jagger map identified more QTLs (40 QTLs) than the IWGSCv2.1 map (30 QTLs). The future direction of these studies will be marker development to utilize these QTLs in yield-improvement breeding programs.

Genetic Analysis of End-use Quality Traits in Soft White Wheat (Triticum Aestivum L.)

Author : Kendra Lyn Gregory Jernigan
Publisher :
ISBN 13 :
Total Pages : 158 pages
Book Rating : 4.:/5 (956 download)

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Book Synopsis Genetic Analysis of End-use Quality Traits in Soft White Wheat (Triticum Aestivum L.) by : Kendra Lyn Gregory Jernigan

Download or read book Genetic Analysis of End-use Quality Traits in Soft White Wheat (Triticum Aestivum L.) written by Kendra Lyn Gregory Jernigan and published by . This book was released on 2015 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat (Triticum aestivum L.) is used in diverse baked products that require specific end use quality traits. Kernel texture, flour water absorption capacity, gluten strength, starch composition, and other flour constituents all influence overall flour functionality and dough rheology, specifying both wheat market class and intended end product. Wheat breeders need to develop cultivars with superior end-use quality traits, while also optimizing important agronomic traits. Our first objective was to use a genetic linkage map and 207 recombinant inbred lines (RIL) from a soft white 'Coda' by 'Brundage' cross to identify quantitative trait loci (QTL) for grain, milling, and baking traits. The linkage map was developed using 570 single nucleotide polymorphisms (SNP) and 136 simple sequence repeat markers. The RILs were grown in five locations in Idaho and Washington from 2006 to 2013. We detected three QTL on chromosomes 2D, 4B, and 6B that were consistently associated with multiple end-use quality traits. Our second objective was to use a genetic linkage map and 131 RILs from a soft white 'Louise' by 'Alpowa' cross to identify QTL associated with arabinoxylan content and milling traits. The linkage map consisted of 924 SNPs and 41 linkage groups. This population was grown in three Washington locations from 2011 to 2012. We detected 28 QTL associated with seven arabinoxylan content and milling traits. Our third objective was to use 480 advanced breeding lines and Pacific Northwest cultivars to identify molecular markers associated with 21 end-use quality traits. Genotypic data from the iSelect 90K SNP chip was combined with best linear unbiased predictions of historic phenotypic data from the USDA-ARS Western Wheat Quality Laboratory. Genome-wide association mapping in the R package, genome association and prediction integrated tool (GAPIT), detected significant markers for multiple end-use quality traits on chromosomes1B, 1D, 2D, 5A, 5B, and 7A. An improved understanding of the genetic architecture underlying end-use quality traits in wheat may assist breeders with cultivar development for superior end-use quality, particularly by increasing frequencies of favorable alleles in breeding populations. Cultivars with superior end-use quality will allow US wheat producers to maintain domestic and international markets.

Use of Barley and Wheat Reference Sequences: Downstream Applications in Breeding, Gene Isolation, GWAS and Evolution, Volume II

Author : Dragan Perovic
Publisher : Frontiers Media SA
ISBN 13 : 2832507832
Total Pages : 260 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Use of Barley and Wheat Reference Sequences: Downstream Applications in Breeding, Gene Isolation, GWAS and Evolution, Volume II by : Dragan Perovic

Download or read book Use of Barley and Wheat Reference Sequences: Downstream Applications in Breeding, Gene Isolation, GWAS and Evolution, Volume II written by Dragan Perovic and published by Frontiers Media SA. This book was released on 2022-11-30 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146'

Download Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146' PDF Online Free

Author : Izabela L. Ciechanowska
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)

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Book Synopsis Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146' by : Izabela L. Ciechanowska

Download or read book Quantitative Trait Loci Mapping of Rust Resistance and Agronomic Traits in the Doubled Haploid Spring Wheat Population 'HYAYT12-10' × 'GP146' written by Izabela L. Ciechanowska and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Marker-assisted selection requires the identification of molecular markers associated with major genes and quantitative trait loci (QTL) using linkage analysis. In this study, we used 167 doubled haploid (DH) lines derived from two unregistered spring wheat (Triticum aestivum L.) parental lines that belong to the Canada Western Special Purpose (CWSP) class to map QTLs associated with five traits using inclusive composite interval mapping (ICIM). Using ICIM, least square means phenotype data across 3-4 environments, and a genetic map of 2,676 SNPs out of the wheat 90K SNP array, we identified ten QTLs associated with maturity (4A and 5B), plant lodging (4B, 5A, 5D, and 7D), grain yield (2D), leaf rust (4A) and stem rust (1A and 2B). Each QTL individually accounted for 6.0-22.3% of the phenotypic variance and together accounted for 8.6-38.2% of each trait. QTLs identified for rusts using ICIM had a minor effect (6.0-9.0%) or a major effect (22.3%). Our major effect QTL at 22.3% was discovered on chromosome 2B and contributed to stem rust response. Its physical location has been associated with disease response in previous studies. Results from this study provide additional valuable information to wheat researchers, in particular that the area on chromosome 2B should be considered for future analyses.

The Discovery, Validation, and Fine Mapping of Stripe Rust Resistance QTL in a Hexaploid Wheat Mapping Population

Author : Iago Zebediah Lowe
Publisher :
ISBN 13 : 9781267029041
Total Pages : pages
Book Rating : 4.0/5 (29 download)

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Book Synopsis The Discovery, Validation, and Fine Mapping of Stripe Rust Resistance QTL in a Hexaploid Wheat Mapping Population by : Iago Zebediah Lowe

Download or read book The Discovery, Validation, and Fine Mapping of Stripe Rust Resistance QTL in a Hexaploid Wheat Mapping Population written by Iago Zebediah Lowe and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The bane of wheat-based agricultural societies for millennia, the rust diseases continue to pose the single largest biotic threat to global wheat production despite more than a century of systematic scientific work on this important pathosystem. Recent breaches of numerous widely deployed sources of resistance have brought the wheat rusts once more to the center of global agricultural concerns and urge us to consider how, aided by new tools in molecular genetics and genomics, we may one day develop truly durable strategies of resistance to these sophisticated and evolving pathogens. Chapter 1 provides an historical overview of wheat rust resistance and argues that a new conceptual framework, one prioritizing the functional classification of quantitative resistance genes, is needed if the objective of developing deployable strategies of durable rust resistance is to be met. Chapter 2 details the identification and validation of stripe rust resistance QTL segregating in a hexaploid wheat mapping population. By focusing on sources of partial resistance, phenotyping under pressure from a broadly-virulent pathogen population in the field, characterizing the mapping population over multiple seasons, and validating the discovered QTL in independent populations, this study models the approach to rust resistance QTL identification and validation outlined in the first chapter. Chapter 3 presents the reasons for prioritizing continued investment in Yr48, a non-major resistance gene identified in the described mapping population. This discussion is followed by further phenotypic characterization of Yr48 and dissection of its validated QTL region, work which sets the stage for the construction of a physical map of the Yr48 region and the positional cloning of this novel source of resistance. The final section of this dissertation outlines the likely future research activities motivated by the results presented in the previous chapters. While it may be several years before Yr48 is cloned, this dissertation provides a solid justification and foundation for that work. And through its eventual identification and functional characterization, Yr48 promises to advance our understanding of the mechanisms of quantitative resistance to wheat stripe rust and thereby facilitate the long-term goal of engineering strategies of durable resistance to this historic agricultural pathogen.

Comparative QTL Mapping of Hagberg Falling Number, Pre-harvest Sprouting, and Late Maturity Alpha-amylase in UI Platinum by SY Capstone Derived Population

Download Comparative QTL Mapping of Hagberg Falling Number, Pre-harvest Sprouting, and Late Maturity Alpha-amylase in UI Platinum by SY Capstone Derived Population PDF Online Free

Author : Jacob G. Bevan
Publisher :
ISBN 13 :
Total Pages : 118 pages
Book Rating : 4.6/5 (721 download)

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Book Synopsis Comparative QTL Mapping of Hagberg Falling Number, Pre-harvest Sprouting, and Late Maturity Alpha-amylase in UI Platinum by SY Capstone Derived Population by : Jacob G. Bevan

Download or read book Comparative QTL Mapping of Hagberg Falling Number, Pre-harvest Sprouting, and Late Maturity Alpha-amylase in UI Platinum by SY Capstone Derived Population written by Jacob G. Bevan and published by . This book was released on 2020 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Hagberg falling number (FN) is an important quality trait in common wheat being used in grain trading business and in end-use quality due to starch damage. Measuring FN can is conducted using flour sample after grain is harvested. Therefore, assessment of FN is very expensive and affected by environments where the grains were produced. Pre-harvest sprouting (PHS) and late maturity Îł-Amylase (LMA) are the two main sources that cause wheat grain samples with low FN. QTL and tightly linked molecular markers for FN, PHS, and LMA is the best alternative that can be used in early generation selection and improvement of selection efficiency. The present study conducted a comparative QTL mapping for the three related traits in a doubled haploid population derived from two hard white spring wheat cultivars UI Platinum (UIP) and SY Capstone (SYC). FN data from nine trials, PHS data from eight datasets in two experiments, and LMA data from two treatment trials were used in QTL analysis. A total of sixteen QTL for FN were detected on seven chromosome regions and five of them (QFN.UIA-1B-1, QFN.UIA-2B, QFN.UIA-3B-1, QFN.UIA-5A-1, QFN.UIA-7A-1) were detected in three or more data sets, explaining 10 to 34% of phenotypic variation. Nine QTL for PHS were identified in three or more data sets on six chromosome regions, explaining 10 to 25% of phenotypic variation. QTL for LMA were identified on chromosomes 4A (UIP HFN), 5A (UIP HFN), and 7A-1 (SYC HFN). QTL on 5A was associated with all three traits and UIP contributed to higher FN, resistance to PHS and LMA. QTL on 7A-1 was associated with FN and LMA and UIP contributed to lower FN and higher PHS score. QTL on 1B-1 was associated with FN and PHS and UIP contributed to the lower FN and higher PHS score. This study suggests that the three traits may share a little genetic information in common, therefore, selecting one trait may be able to indirectly select other related traits. This study also suggests that resistant lines to FN, PHS, and LMA can be selected through genetic recombination for QTL from the two parents. Compared to the physical map of Chinese Spring, the 5A QTL is the flanking region of Amy 3 gene. This suggests that it is necessary to study the Amy 3 gene in order to understand the genetic control of the three traits assessed in the present study.

Molecular Mapping of Rust Resistant Qtls in a Synthetic Hexaploid Wheat Population

Author : Jeffrey David Kovach
Publisher :
ISBN 13 : 9780355408058
Total Pages : 106 pages
Book Rating : 4.4/5 (8 download)

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Book Synopsis Molecular Mapping of Rust Resistant Qtls in a Synthetic Hexaploid Wheat Population by : Jeffrey David Kovach

Download or read book Molecular Mapping of Rust Resistant Qtls in a Synthetic Hexaploid Wheat Population written by Jeffrey David Kovach and published by . This book was released on 2017 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Characterization of Durable Yellow and Leaf Rust Resistance in Two Wheat Populations

Author : Bhoja Basnet
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (813 download)

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Book Synopsis Molecular Characterization of Durable Yellow and Leaf Rust Resistance in Two Wheat Populations by : Bhoja Basnet

Download or read book Molecular Characterization of Durable Yellow and Leaf Rust Resistance in Two Wheat Populations written by Bhoja Basnet and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat (Triticum aestivum L.) is one of the most important food crops, comprising the largest source of daily calorie and protein intake of human beings worldwide. Among the several diseases of wheat, Yellow Rust (YR; caused by Puccinia striiformis Westend. f. sp. tritici) and Leaf Rust (LR; caused by Puccinia triticina Erikss. & Henn.) have always been major production constraints since the domestication of wheat. For the last few decades, scientists have invested large efforts to identify, characterize and utilize Adult Plant Resistance (APR), a.k.a. slow rusting resistance, in wheat germplasm to promote durability of resistance against rust. The objectives of this study were to 1) understand the genetics of APR to YR and/or LR present in two potential wheat lines 'Quaiu 3' and 'TAM 111', and 2) map the putative Quantitative Trait Loci (QTL) associated with YR and LR resistance using DNA-based molecular markers. Two Recombinant Inbred Line (RIL) populations were subjected to YR and LR disease evaluation experiments in multiple years and locations. Visual evaluation of Disease severity (DS) and Infection Type (IT) score in both RIL populations showed that APR to YR and LR were highly heritable quantitative traits with significant correlation among experiments. In spring wheat population, composite interval mapping consistently detected four and three large effect QTL for YR and LR resistance, respectively. Among those QTLs, 1B, 3B and 1D QTL were found to be associated with previously characterized genes Lr46/Yr29, Sr2/Yr30 and Lr42, respectively. However, QTLs QYr.tam-3D and QYr.tam-2D were potentially novel. The largest YR QTL QYr.tam-2D was located on long arm of chromosome 2D explaining about 48 to 61% of the total phenotypic variation. Similarly, in winter wheat population, apart from three environment-specific QTL on chromosomes 1A, 2A and 7D, the QTL on chromosome 2B (QYr.tam-2B) was found to express consistently in multiple environments explaining about 23 to 63% of total phenotypic variation. This study has further elucidated the inheritance mechanism of APR to YR and LR present in two different wheat lines, Quaiu 3 and TAM 111, and resulted in the successful mapping and characterization of the genetic loci associated with corresponding disease resistance traits. These findings should be very useful to isolate the novel APR genes and/or directly use in wheat breeding programs to enhance durable rust resistance in diverse wheat germplasm and cultivars in the future.

Development of DNA Markers for Wheat Quality Traits in CPS White Wheat Using Quantitative Trait Loci (QTL) Analysis : Final Report

Author : Stephen L. (Stephen Lindsay) Fox
Publisher : [Regina] : Saskatchewan Agriculture Development Fund
ISBN 13 :
Total Pages : 17 pages
Book Rating : 4.:/5 (174 download)

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Book Synopsis Development of DNA Markers for Wheat Quality Traits in CPS White Wheat Using Quantitative Trait Loci (QTL) Analysis : Final Report by : Stephen L. (Stephen Lindsay) Fox

Download or read book Development of DNA Markers for Wheat Quality Traits in CPS White Wheat Using Quantitative Trait Loci (QTL) Analysis : Final Report written by Stephen L. (Stephen Lindsay) Fox and published by [Regina] : Saskatchewan Agriculture Development Fund. This book was released on 2003 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Genetic Mapping of Quantitative Trait Loci for Slow-rusting Traits in Wheat

Author : Yue Lu
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (95 download)

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Book Synopsis Genetic Mapping of Quantitative Trait Loci for Slow-rusting Traits in Wheat by : Yue Lu

Download or read book Genetic Mapping of Quantitative Trait Loci for Slow-rusting Traits in Wheat written by Yue Lu and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Wheat leaf rust, caused by Puccinia triticina, is an important fungal disease worldwide. Growing resistant cultivars is an effective practice to reduce the losses caused by the disease, and using slow-rusting resistance genes can improve the durability of rust resistance in the cultivars. CI13227 is a winter wheat line that shows a high level of slow-rusting resistance to leaf rust and has been studied extensively. In this research, two recombinant inbreed line (RIL) populations derived from CI13227 x Suwon (104 RILs) and CI13227 x Everest (184 RILs) and one doubled haploid (DH) population derived from CI13227 x Lakin with 181 lines were used to identify quantitative trait loci (QTLs) for slow leaf rusting resistance. Each population and its parents were evaluated for slow-rusting traits in two greenhouse experiments. A selected set of 384 simple sequence repeat markers (SSRs), single nucleotide polymorphism markers (SNPs) derived from genotyping-by-sequencing (GBS-SNPs) or 90K-SNP chip (90K-SNPs) were analyzed in the three populations. Six QTLs for slow-rusting resistance, QLr.hwwgru-2DS, QLr.hwwgru-7BL, QLr.hwwgru-7AL, QLr.hwwgru-3B_1, QLr.hwwgru-3B_2, and QLr.hwwgru-1D were detected in the three populations with three stable QTLs, QLr.hwwgru-2DS, QLr.hwwgru-7BL and QLr.hwwgru-7AL. These were detected and validated by Kompetitive Allele-Specific PCR (KASP) markers converted from GBS-SNPs and 90K-SNPs in at least two populations. Another three QTLs were detected only in a single population, and either showed a minor effect or came from the susceptible parents. The KASP markers tightly linked to QLr.hwwgru-2DS (IWB34642, IWB8545 and GBS_snpj2228), QLr.hwwgru-7BL (GBS_snp1637 and IWB24039) and QLr.hwwgru-7AL (IWB73053 and IWB42182) are ready to be used in marker-assisted selection (MAS) to transfer these QTLs into wheat varieties to improve slow-rusting resistance in wheat.

Genetics of Resistance to Leaf and Stripe Rust Diseases in the Spring Wheat 'Amadina'

Author : Peter Michael Bulli Nyori
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (656 download)

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Book Synopsis Genetics of Resistance to Leaf and Stripe Rust Diseases in the Spring Wheat 'Amadina' by : Peter Michael Bulli Nyori

Download or read book Genetics of Resistance to Leaf and Stripe Rust Diseases in the Spring Wheat 'Amadina' written by Peter Michael Bulli Nyori and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In this research, a recombinant inbred line (RIL) population derived from cross between a leaf rust- and stripe rust-susceptible spring wheat 'Avocet S' and a slow leaf- and stripe-rusting resistant spring wheat 'Amadina' was used to postulate and map leaf rust seedling resistance genes, identify quantitative trait loci (QTL) for slow-rusting resistance against leaf and stripe rust, and study slow leaf-rusting components, latent period and infection frequency. Two known Lr genes (Lr23, and Lr26) were identified to be present in 'Amadina' through gene postulation, pedigree, cytogenetic, and polymerase chain reaction analyses. One unknown gene associated with seedling resistance was also mapped on chromosome 1BL. In greenhouse experiment, it was estimated that at least five genes conditioning final disease severity (FS) and latent period (LP), and four genes conditioning infection frequency (IF), segregated in the population. Correlations between LP and FS, and LP and IF were moderately negative, and that between IF and FS was moderately positive, indicating inter-dependence of the traits. Two QTL on chromosomes 1BL and 6BL were associated with LP and FS, and three QTL on chromosomes 1BL, 6BL and 2DS were associated with IF. Segregation of the RIL population in field experiment indicated that there were at least four and three adult plant resistance (APR) genes involved in resistance for leaf and stripe rust. Six QTL on chromosomes 3AL, 4AL, 1BL, 5BL, and 7BL were associated with APR for leaf rust, and seven QTL on chromosome 4AL, 5AL, 1BL, 2BL, 4BL, 5BL, 2DL, and 4D were associated with APR for stripe rust. Our results indicated that the major portion of genetic variability for slow-rusting resistance was additive gene action, and, to some extent, epistasis. In this research, we also explored the utility of remote sensing and geographic information systems (GIS) and analytical operations to discriminate leaf rust pustules from other parts of leaf and to accurately determine pustule size in 'Amadina' and 'Avocet S'.

QTL Mapping of Stem Rust Resistance Loci in Durum Wheat Populations

Author : Shitaye Homma Megerssa
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (14 download)

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Book Synopsis QTL Mapping of Stem Rust Resistance Loci in Durum Wheat Populations by : Shitaye Homma Megerssa

Download or read book QTL Mapping of Stem Rust Resistance Loci in Durum Wheat Populations written by Shitaye Homma Megerssa and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem rust caused by Puccinia graminis f. sp. tritici Eriks. & Henn is the most destructive disease of durum and common wheat. The main focus of this study is to identify loci associated with stem rust resistance in durum wheat using association mapping and linkage mapping. A panel of 283 lines and 224 recombinant inbred lines (RILs) from a cross between 'Reichenbachii' and 'DAKIYE' developed by the durum wheat breeding program of the International Maize and Wheat Improvement Center (CIMMYT) were used for the study. The panel was evaluated against races TTKSK, TKTTF, JRCQC and TTRTF at the seedling stage and TKTTF and JRCQC in the field in Ethiopia from 2018 to 2019 for two seasons. The same panel was evaluated against bulk of multiple stem rust races prevalent in Ethiopia and Kenya from 2018 to 2019 in five environments. Genome-wide association study (GWAS) was conducted using 26,439 single nucleotide polymorphism (SNP) markers for seedling response (280 lines) and field response (283 lines) to stem rust. The RILs along with the two parents were evaluated for response to bulk of multiple stem rust races in Ethiopia and Kenya for two seasons from 2019 to 2020. Linkage analyses were conducted using 843 SNP markers for 175 lines. For GWAS of seedling response, a mixed linear model (MLM) identified 17 quantitative trait loci (QTL) of which eight were putatively novel while FarmCPU identified 20 QTL and 12 were likely novel. For field resistance to races TKTTF and JRCQC, MLM detected 19 QTL of which 12 were likely novel while FarmCPU detected 16 QTL and seven were putatively novel. For resistance to multiple Pgt races in East Africa, 160 significant marker-trait associations (MTAs) grouped into 42 QTL were identified using MLM and FarmCPU and 21 QTL were likely novel. From previously reported Sr genes, the regions of Sr7a, Sr8a, Sr8155B1, Sr11, Sr12, alleles of Sr13, Sr17, Sr22/Sr25, and Sr49 were identified. For the biparental population, composite interval mapping (CIM) identified three QTL on chromosomes 3B (QSr.cnl-3B), 4B (QSr.cnl-4B) and 7B (QSr.cnl-7B). These three QTL contributed by the resistant parent explained 4.7% to 15.3% of the phenotypic variation and all match previously reported loci. Lines with multiple-race stem rust resistance can be used as parents in durum wheat resistance breeding to stem rust and markers identified in the GWAS can be used in marker-assisted selection (MAS) once validated in a different population. Further study on the validation of allele specific markers and allelism tests in the Sr13 region of chromosome 6A is needed. Future evaluation of large numbers of durum wheat lines and searching for durable adult plant resistance gene is crucial in resistance breeding of durum wheat.

Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest

Download Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest PDF Online Free

Author : Lu Liu
Publisher :
ISBN 13 :
Total Pages : 193 pages
Book Rating : 4.:/5 (111 download)

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Book Synopsis Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest by : Lu Liu

Download or read book Molecular Mapping and Characterization of Stripe Rust Resistance Genes in Important Winter Wheat Cultivars in the U.S. Pacific Northwest written by Lu Liu and published by . This book was released on 2018 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), is one of the most devastating and economically important diseases across major wheat growing regions in the world. Growing resistant cultivars is the most effective and environmentally sound method for controlling the disease. This study was conducted to identify resistance genes/QTL in three winter wheat cultivars Madsen, Skiles and Eltan and develop molecular markers for marker-assisted selection. Madsen has shown high-level and durable resistance since its release in 1988 and was identified to have both all-stage resistance (ASR) and high-temperature adult-plant (HTAP) resistance. Two major effect ASR QTL on chromosome arms 1AS and 2AS, one minor ASR QTL on 1BS and two HTAP resistance QTL on 3BS and 6BS were identified in Madsen. Skiles is susceptible to all tested Pst races at seedling stage but shows strong resistance at adult-plant stage with higher resistance level expressed at high temperature, indicating that Skiles has only HTAP resistance against the tested Pst races. Six HTAP resistance QTL were mapped in Skiles, with two major QTL mapped on 3BS and 4BL and four minor QTL on 1BL, 5AL, 6B and 7DL. Markers linked to the QTL were used to select highly resistant breeding lines derived from crosses with Skiles. Eltan has shown variable reactions from moderate susceptibility to resistance in different years since its release in 1990. Five QTL were detected in Eltan, with two major ASR QTL on 2BS and 4BL, one minor ASR on 5BS and two minor HTAP resistance QTL on 6AS and 7BL. The two major ASR QTL became less effective due to the fact that the Pst population has changed to virulent, leading to the resistance reduction in Eltan. By studying these three wheat cultivars with different genes for different types of resistance, it is concluded that the best strategy to achieve high-level and durable resistance is to combine several genes for effective ASR and adequate HTAP resistance. These studies provide the information of useful genes and molecular markers for improving resistance to stripe rust in wheat cultivars.