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Protocols And Applications In Enzymology
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Book Synopsis Protocols and Applications in Enzymology by : Seema Anil Belorkar
Download or read book Protocols and Applications in Enzymology written by Seema Anil Belorkar and published by Academic Press. This book was released on 2021-11-19 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards Presents easy to read, reproducible protocols for researchers and students across academia and industry Includes color diagrams that illustrate key concepts
Book Synopsis Enzyme Engineering by : James Samuelson
Download or read book Enzyme Engineering written by James Samuelson and published by Humana Press. This book was released on 2016-05-01 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.
Book Synopsis Single Molecule Enzymology by : Gregory I. Mashanov
Download or read book Single Molecule Enzymology written by Gregory I. Mashanov and published by Humana Press. This book was released on 2016-08-23 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
Book Synopsis Enzyme Stabilization and Immobilization: Methods and Protocols by : Shelley D. Minteer
Download or read book Enzyme Stabilization and Immobilization: Methods and Protocols written by Shelley D. Minteer and published by Methods in Molecular Biology. This book was released on 2018-11-05 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Non-Aqueous Enzymology by : Munishwar N. Gupta
Download or read book Methods in Non-Aqueous Enzymology written by Munishwar N. Gupta and published by Birkhäuser. This book was released on 2013-12-01 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.
Book Synopsis Enzyme Stabilization and Immobilization by : Shelley D. Minteer
Download or read book Enzyme Stabilization and Immobilization written by Shelley D. Minteer and published by Humana Press. This book was released on 2011-04-06 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.
Book Synopsis Practical Enzymology by : Hans Bisswanger
Download or read book Practical Enzymology written by Hans Bisswanger and published by John Wiley & Sons. This book was released on 2019-11-06 with total page 533 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Book Synopsis Multienzymatic Assemblies by : Haralambos Stamatis
Download or read book Multienzymatic Assemblies written by Haralambos Stamatis and published by Humana. This book was released on 2023-06-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
Book Synopsis Laboratory Methods in Enzymology: Protein Part B by :
Download or read book Laboratory Methods in Enzymology: Protein Part B written by and published by Academic Press. This book was released on 2014-03-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.
Book Synopsis Laboratory Methods in Enzymology by : Jon Lorsch
Download or read book Laboratory Methods in Enzymology written by Jon Lorsch and published by . This book was released on 2013 with total page 413 pages. Available in PDF, EPUB and Kindle. Book excerpt: These volumes of Methods in Enzymology contain the protocols that made up the on-line Methods Navigator. Our philosophy when we selected the protocols to include in the Navigator was that they should be for techniques useful in any biomedical laboratory, regardless of the system the lab studies. Each protocol was written by researchers who use the technique routinely, and in many cases by the people who actually developed the procedure in the first place. The protocols are very detailed and contain recipes for the necessary buffers and reagents, as well as flow-charts outlining the steps involved. Many of the chapters have accompanying videos demonstrating key parts of the procedures. In a few cases, detailed protocols for certain important approaches could not be generated either because they are instrument-specific (e.g., next-generation sequencing) or because they are proprietary (e.g., column-based nucleic acid purifications). In these cases we have included "explanatory chapters" that outline the theoretical basis for each technique without giving a detailed protocol. The volumes are broken into distinct areas: DNA methods; Cell-based methods; lipid, carbohydrate and miscellaneous methods; RNA methods; protein methods. Our goal is that these protocols will be useful for everyone in the lab, from undergraduates and rotation students to seasoned post-doctoral fellows. We hope that these volumes will become dog-eared and well-worn in your laboratory, either physically or electronically
Download or read book Enzymes written by Selim Kermasha and published by Academic Press. This book was released on 2020-11-27 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes: Novel Biotechnological Approaches for the Food Industry provides an in-depth background of the most up-to-date scientific research and information related to food biotechnology and offers a wide spectrum of biological applications. This book addresses novel biotechnological approaches for the use of enzymes in the food industry to help readers understand the potential uses of biological applications to advance research. This is an essential resource to researchers and both undergraduate and graduate students in the biotechnological industries. Provides fundamental and rigorous scientific information on enzymes Illustrates enzymes as tools to achieve value and quality to a product, either in vitro or in vivo Presents the most updated knowledge in the area of food biotechnology Demonstrates novel horizons and potential for the use of enzymes in industrial applications
Book Synopsis Immobilization of Enzymes and Cells by : Jose M. Guisan
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana Press. This book was released on 2013-08-11 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Book Synopsis Immobilization of Enzymes and Cells by : Jose M. Guisan
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2021-01-29 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Book Synopsis Lipases and Phospholipases by : Georgina Sandoval
Download or read book Lipases and Phospholipases written by Georgina Sandoval and published by Humana. This book was released on 2019-12-10 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
Book Synopsis Microbial Enzymes and Biotransformations by : Jose Luis Barredo
Download or read book Microbial Enzymes and Biotransformations written by Jose Luis Barredo and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.
Book Synopsis Biocatalysis for Practitioners by : Gonzalo de Gonzalo
Download or read book Biocatalysis for Practitioners written by Gonzalo de Gonzalo and published by John Wiley & Sons. This book was released on 2021-07-19 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Book Synopsis Understanding Enzymes by : Trevor Palmer
Download or read book Understanding Enzymes written by Trevor Palmer and published by . This book was released on 1985 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: This clear and lucid book helps towards an understanding of the principles of enzymology, a subject with a somewhat undeserved reputation for being "difficult".
